DK2825631T3 - Fremstilling af docosahexaensyre og astaxanthin i mixotrof modus under anvendelse af schizochytrium - Google Patents
Fremstilling af docosahexaensyre og astaxanthin i mixotrof modus under anvendelse af schizochytrium Download PDFInfo
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- DK2825631T3 DK2825631T3 DK13715330.0T DK13715330T DK2825631T3 DK 2825631 T3 DK2825631 T3 DK 2825631T3 DK 13715330 T DK13715330 T DK 13715330T DK 2825631 T3 DK2825631 T3 DK 2825631T3
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- schizochytrium
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- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
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Claims (11)
1. Fremgangsmåde omfattende det følgende trin: a) dyrkning i mixotrof modus af en eller flere stammer fra slægten Schizochytrium under forhold af belysning, der er diskontinuert og/eller variabel over tid, hvor belysningen har variationer i intensitet mellem belyste faser og mørke faser, hvor amplituden ligger mellem 30 pmol-nr^s" 1 og 1000 pmol-nr^s'1, idet disse variationer finder sted mellem 2 og 3600 gange i timen.
2. Fremgangsmåde ifølge krav 1, kendetegnet ved at belysningen har variationer i intensitet, hvor amplituden ligger mellem 30 pmol-nr^s1 og 400 pmol-nr^s'1, idet disse variationer finder sted mellem 2 og 200 gange i timen.
3. Fremgangsmåde ifølge krav 1 eller 2, kendetegnet ved at dyrkningen udføres i tilstedeværelse af et organisk karbonholdigt substrat i en koncentration på fra 100 mM til 1,5 M, fortrinsvis fra 300 mM til 1,2 M, mere fortrinsvis fra 500 mM til 1 M, og endnu mere fortrinsvis fra 600 mM til 900 mM, hvor det organiske karbonholdige substrat er valgt blandt saccharose, glycerol, glucose og cellulosederivater og en blanding af disse molekyler.
4. Fremgangsmåde ifølge krav 3, kendetegnet ved at det organiske karbonholdige substrat, der er til stede i dyrkningsmediet, omfatter mindst 300 mM glucose og/eller glycerol.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1-4, kendetegnet ved at amplituden af variationerne i intensitet ligger mellem 30 og 1000 pmol-m^-s"1, fortrinsvis mellem 70 og 300 pmol-m'^s'1, og mere fortrinsvis mellem 100 og 200 pmol-nr^s'1.
6. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 5, kendetegnet ved at lystilførslen er i form af blitzglimt.
7. Fremgangsmåde ifølge krav 6, kendetegnet ved at et blitzglimt har en varighed liggende mellem 1/10 sekund og 10 minutter, fortrinsvis 5 sekunder og 10 minutter, mere fortrinsvis mellem 10 sekunder og 2 minutter, mere fortrinsvis mellem 20 sekunder og 1 minut.
8. Fremgangsmåde ifølge krav 6 eller krav 7, kendetegnet ved at antallet af blitzglimt er mellem 5 og 3600, fortrinsvis mellem 10 og 150, fortrinsvis mellem 15 og 100, og mere fortrinsvis mellem 20 og 50 gange i timen.
9. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 6, kendetegnet ved at den samlede lystilførsel per time i mikromol fotoner er mellem 2000 til 600000, fortrinsvis mellem 2000 til 200000 pmol-nr2.
10. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 9, kendetegnet ved at den yderligere omfatter de følgende trin: b) et trin til at fastholde den nævnte kultur over flere generationer i tilstedeværelse af et organisk karbonholdingt substrat i dyrkningsmediet, og eventuelt c) et trin til at udvinde de derved dyrkede protister, og eventuelt d) et trin at udvinde det hydrofobe materiale, og eventuelt e) ekstraktion af DHA (docosahexaensyre) og astaxanthin fra det udvundne hydrofobe materiale.
11. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 10, kendetegnet ved at protisten fra slægten Schizochytrium svarer til stammen FCC 1104, deponeret hos CCAP (Culture Collection of Algae and Protozoa), under nummer CCAP 4087/1.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1252380A FR2988100B1 (fr) | 2012-03-16 | 2012-03-16 | Production d'acide docosahexaenoique et d'astaxanthine en mode mixotrophe par schizochytrium |
| PCT/FR2013/050547 WO2013136028A1 (fr) | 2012-03-16 | 2013-03-15 | Production d'acide docosahexaénoïque et d'astaxanthine en mode mixotrophe par schizochytrium. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2825631T3 true DK2825631T3 (da) | 2017-12-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK13715330.0T DK2825631T3 (da) | 2012-03-16 | 2013-03-15 | Fremstilling af docosahexaensyre og astaxanthin i mixotrof modus under anvendelse af schizochytrium |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9506100B2 (da) |
| EP (1) | EP2825631B1 (da) |
| JP (1) | JP6352819B2 (da) |
| CN (1) | CN104185678A (da) |
| DK (1) | DK2825631T3 (da) |
| ES (1) | ES2650440T3 (da) |
| FR (1) | FR2988100B1 (da) |
| HU (1) | HUE035610T2 (da) |
| IN (1) | IN2014DN08166A (da) |
| PL (1) | PL2825631T3 (da) |
| WO (1) | WO2013136028A1 (da) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6298770B2 (ja) * | 2013-01-18 | 2018-03-20 | 協和発酵バイオ株式会社 | ドコサヘキサエン酸を生産する微生物及びその利用 |
| FR3008423B1 (fr) * | 2013-07-12 | 2017-12-22 | Fermentalg | Nouvelle souche de aurantiochytrium |
| FR3019559B1 (fr) * | 2014-04-03 | 2018-01-05 | Fermentalg | Procede de culture des microalgues du genre aurantiochytrium dans un milieu de culture a teneur reduite en sodium et en chlorure pour la production de dha |
| EP3385359A4 (en) | 2015-12-01 | 2019-07-24 | Nippon Suisan Kaisha, Ltd. | DOCOSAHEXAIC ACID-CONTAINING OIL AND METHOD FOR THE PRODUCTION THEREOF |
| FR3045069B1 (fr) * | 2015-12-14 | 2019-01-25 | Metabolium | Procede d'enrichissement de protistes en lipides riches en acides gras polyinsatures, plus particulierement de classe omega 3, et sa mise en oeuvre pour la production de ces lipides |
| KR20200019111A (ko) * | 2017-02-22 | 2020-02-21 | 카아길, 인코포레이팃드 | Dha 함유 오일의 정제 |
| FR3085825B1 (fr) | 2018-09-14 | 2021-07-16 | Fermentalg | Huile de microorganismes riche en acide docosahexaenoique |
| FR3085962B1 (fr) | 2018-09-14 | 2021-06-18 | Fermentalg | Procede d'extracton d'une huile riche en pufa |
| CN109913513B (zh) * | 2018-11-29 | 2020-10-09 | 厦门大学 | 一种驯化裂殖壶菌产油脂的方法 |
| CN114015575A (zh) * | 2021-11-30 | 2022-02-08 | 广西源藻生物科技有限公司 | 一种基于微藻闪光效应的微藻规模化培养方法 |
| CN114703238B (zh) * | 2022-04-08 | 2023-12-01 | 山东省食品发酵工业研究设计院 | 一种裂殖壶菌产二十二碳六烯酸发酵方法及其应用 |
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|---|---|---|---|---|
| US3444647A (en) * | 1961-08-08 | 1969-05-20 | Masahito Takahashi | Process of cultivating algae |
| US3316674A (en) * | 1964-09-11 | 1967-05-02 | Yakult Honsha Kk | Method of new industrial cultivation of unicellular green algae such as chlorella |
| US5381075A (en) * | 1992-03-20 | 1995-01-10 | Unisyn | Method and apparatus for driving a flashing light systems using substantially square power pulses |
| JPH0889234A (ja) * | 1994-09-30 | 1996-04-09 | Mitsubishi Heavy Ind Ltd | 光合成生物の培養方法 |
| AUPN060095A0 (en) * | 1995-01-13 | 1995-02-09 | Enviro Research Pty Ltd | Apparatus for biomass production |
| JP2003052357A (ja) * | 2001-08-10 | 2003-02-25 | Japan Science & Technology Corp | 海洋性微生物と、この微生物を用いたカロテノイド系色素および／または高度不飽和脂肪酸の製造方法 |
| AU2003903453A0 (en) * | 2003-07-07 | 2003-07-17 | The University Of Queensland | Production of hydrogen |
| ES2626018T3 (es) * | 2005-06-07 | 2017-07-21 | Dsm Nutritional Products Ag | Microorganismos eucariotas para la producción de lípidos y antioxidantes |
| JP2007043909A (ja) * | 2005-08-05 | 2007-02-22 | Yamaha Motor Co Ltd | 培養装置 |
| US8298548B2 (en) * | 2007-07-18 | 2012-10-30 | Solazyme, Inc. | Compositions for improving the health and appearance of skin |
| JP2010530757A (ja) * | 2007-06-22 | 2010-09-16 | アルゲダイン コーポレイション | バイオリアクター |
| EP2265724A4 (en) * | 2008-04-09 | 2013-01-23 | Solazyme Inc | Direct chemical modification of microbial biomass and microbial oils |
| MY143769A (en) * | 2008-04-30 | 2011-07-15 | Ho Tet Shin | An apparatus for mass cultivation of microalgae and a method for cultivating the same |
| CN101892160B (zh) * | 2010-01-06 | 2012-10-03 | 吉林省希玛生物科技有限公司 | 一种海洋真菌裂殖壶菌(Schizochytrium)LX0809及其工业应用 |
-
2012
- 2012-03-16 FR FR1252380A patent/FR2988100B1/fr not_active Expired - Fee Related
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2013
- 2013-03-15 JP JP2014561500A patent/JP6352819B2/ja active Active
- 2013-03-15 US US14/385,294 patent/US9506100B2/en active Active
- 2013-03-15 EP EP13715330.0A patent/EP2825631B1/fr active Active
- 2013-03-15 WO PCT/FR2013/050547 patent/WO2013136028A1/fr active Application Filing
- 2013-03-15 PL PL13715330T patent/PL2825631T3/pl unknown
- 2013-03-15 IN IN8166DEN2014 patent/IN2014DN08166A/en unknown
- 2013-03-15 CN CN201380014163.9A patent/CN104185678A/zh active Pending
- 2013-03-15 HU HUE13715330A patent/HUE035610T2/en unknown
- 2013-03-15 ES ES13715330.0T patent/ES2650440T3/es active Active
- 2013-03-15 DK DK13715330.0T patent/DK2825631T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2015509733A (ja) | 2015-04-02 |
| JP6352819B2 (ja) | 2018-07-04 |
| US20150037838A1 (en) | 2015-02-05 |
| HUE035610T2 (en) | 2018-05-28 |
| FR2988100B1 (fr) | 2016-02-05 |
| FR2988100A1 (fr) | 2013-09-20 |
| CN104185678A (zh) | 2014-12-03 |
| PL2825631T3 (pl) | 2018-02-28 |
| ES2650440T3 (es) | 2018-01-18 |
| EP2825631A1 (fr) | 2015-01-21 |
| WO2013136028A1 (fr) | 2013-09-19 |
| EP2825631B1 (fr) | 2017-09-06 |
| IN2014DN08166A (da) | 2015-05-01 |
| US9506100B2 (en) | 2016-11-29 |
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