DK2785829T3 - Automatiseret system til fremstilling af inducerede pluripotente stamceller eller differentierede celler - Google Patents

Automatiseret system til fremstilling af inducerede pluripotente stamceller eller differentierede celler Download PDF

Info

Publication number
DK2785829T3
DK2785829T3 DK12852680.3T DK12852680T DK2785829T3 DK 2785829 T3 DK2785829 T3 DK 2785829T3 DK 12852680 T DK12852680 T DK 12852680T DK 2785829 T3 DK2785829 T3 DK 2785829T3
Authority
DK
Denmark
Prior art keywords
pluripotent stem
automated system
induced pluripotent
cells
stem cells
Prior art date
Application number
DK12852680.3T
Other languages
Danish (da)
English (en)
Inventor
Stephen Chang
Scott Noggle
Kevin Eggan
Susan L Solomon
Original Assignee
New York Stem Cell Found Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New York Stem Cell Found Inc filed Critical New York Stem Cell Found Inc
Application granted granted Critical
Publication of DK2785829T3 publication Critical patent/DK2785829T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0696Artificially induced pluripotent stem cells, e.g. iPS
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/04Flat or tray type, drawers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04Cell isolation or sorting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0607Non-embryonic pluripotent stem cells, e.g. MASC
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Clinical Laboratory Science (AREA)
  • Electromagnetism (AREA)
  • Physics & Mathematics (AREA)
  • Physiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Analytical Chemistry (AREA)
  • Computer Hardware Design (AREA)
DK12852680.3T 2011-12-01 2012-11-30 Automatiseret system til fremstilling af inducerede pluripotente stamceller eller differentierede celler DK2785829T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161565818P 2011-12-01 2011-12-01
US201161580007P 2011-12-23 2011-12-23
US201261700792P 2012-09-13 2012-09-13
PCT/US2012/067417 WO2013082509A1 (en) 2011-12-01 2012-11-30 Automated system for producing induced pluripotent stem cells or differentiated cells

Publications (1)

Publication Number Publication Date
DK2785829T3 true DK2785829T3 (da) 2020-01-27

Family

ID=48536128

Family Applications (2)

Application Number Title Priority Date Filing Date
DK12852680.3T DK2785829T3 (da) 2011-12-01 2012-11-30 Automatiseret system til fremstilling af inducerede pluripotente stamceller eller differentierede celler
DK19203379.3T DK3653698T3 (da) 2011-12-01 2012-11-30 Automatiseret system til fremstilling af inducerede pluripotente stamceller eller differentierede celler

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK19203379.3T DK3653698T3 (da) 2011-12-01 2012-11-30 Automatiseret system til fremstilling af inducerede pluripotente stamceller eller differentierede celler

Country Status (14)

Country Link
US (4) US10273459B2 (https=)
EP (2) EP3653698B1 (https=)
JP (1) JP6257520B2 (https=)
KR (5) KR102631374B1 (https=)
CN (2) CN110724636A (https=)
AU (4) AU2012345676B2 (https=)
BR (2) BR122020005735B1 (https=)
CA (2) CA2857295C (https=)
DK (2) DK2785829T3 (https=)
ES (2) ES3038941T3 (https=)
IL (3) IL299477A (https=)
IN (1) IN2014CN04920A (https=)
WO (1) WO2013082509A1 (https=)
ZA (3) ZA201403945B (https=)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10428309B2 (en) * 2011-12-01 2019-10-01 New York Stem Cell Foundation, Inc. Systems and methods for producing stem cells and differentiated cells
WO2013082509A1 (en) 2011-12-01 2013-06-06 The New York Stem Cell Foundation Automated system for producing induced pluripotent stem cells or differentiated cells
WO2014201412A1 (en) * 2013-06-13 2014-12-18 The New York Stem Cell Foundation Inc. Pluripotent stem cells derived from non-cryoprotected frozen tissue and methods for making and using the same
EP3013941B1 (en) * 2013-06-27 2018-10-31 New York Stem Cell Foundation, Inc. Improved systems and methods for producing stem cells and differentiated cells
US10202568B2 (en) * 2013-08-12 2019-02-12 Invivosciences Inc. Automated cell culture system and method
US20160222355A1 (en) * 2014-12-26 2016-08-04 New York Stem Cell Foundation, Inc. Systems and methods for producing stem cells differentiated cells, and genetically edited cells
WO2016118824A1 (en) 2015-01-22 2016-07-28 Regenerative Medical Solutions, Inc. Markers for differentiation of stem cells into differentiated cell populations
CN108138130A (zh) 2015-08-31 2018-06-08 爱平世股份有限公司 多能干细胞制造系统和生产诱导多能干细胞的方法
CN105567642B (zh) * 2016-02-01 2019-07-12 中国科学院生物物理研究所 一种着色性干皮病人多能干细胞的制备方法
EP3423564A4 (en) 2016-03-03 2019-08-21 New York Stem Cell Foundation, Inc. MICROGLIA DERIVED FROM PLURIPOTENTIAL STEM CELLS AND METHOD FOR THE PRODUCTION AND USE THEREOF
JP2019521715A (ja) * 2016-07-21 2019-08-08 セリアドCelyad 細胞を自動的に独立並列してバッチ処理するための方法および装置
US10373109B2 (en) 2016-08-04 2019-08-06 Fanuc Corporation System and method for iPS cell bank using media
US11259520B2 (en) * 2016-08-04 2022-03-01 Fanuc Corporation Stem cell manufacturing system, stem cell information management system, cell transport apparatus, and stem cell frozen storage apparatus
US10354218B2 (en) 2016-08-04 2019-07-16 Fanuc Corporation System and method for iPS cell bank using internet technology
EP3541922A4 (en) 2016-11-18 2020-06-03 New York Stem Cell Foundation, Inc. MICROFLUIDIC SYSTEM AND METHOD FOR USE THEREOF
EP3556842A4 (en) * 2017-01-20 2020-01-15 FUJIFILM Corporation CELL CULTURE APPARATUS AND METHOD
CA3054119A1 (en) * 2017-02-24 2018-08-30 I Peace, Inc. Cell treatment device, suspension culture vessel, and stem cell induction method
CN115247129A (zh) * 2017-02-27 2022-10-28 田边刚士 细胞处理装置
US20190382706A1 (en) * 2017-02-27 2019-12-19 Koji Tanabe Somatic cell production system
MX2019015505A (es) * 2017-06-30 2020-07-28 Inscripta Inc Metodos, modulos, instrumentos y sistemas de procesamiento de celulas automatizados.
GB2564437B (en) * 2017-07-10 2020-11-04 Univ Brunel Method of testing a gene therapy vector using induced pluripotent stem cells
CA3248436A1 (en) 2017-09-01 2025-02-21 Lonza Walkersville, Inc. END-TO-END AUTOMATION OF CELL THERAPY
EP3687621A4 (en) * 2017-09-30 2021-08-11 Inscripta, Inc. Flow through electroporation instrumentation
CN112292445A (zh) * 2018-06-13 2021-01-29 富士胶片株式会社 信息处理装置、导出方法及导出程序
JP6530577B1 (ja) * 2019-03-20 2019-06-12 剛士 田邊 細胞処理システム及び細胞処理装置
JP7090337B2 (ja) * 2019-03-20 2022-06-24 アイ ピース,インコーポレイテッド 細胞処理システム及び細胞処理装置
JP6530874B1 (ja) * 2019-03-20 2019-06-12 剛士 田邊 細胞処理システム及び細胞処理装置
AU2020358866A1 (en) * 2019-10-04 2022-06-02 New York Stem Cell Foundation, Inc. Imaging system and method of use thereof
WO2021104453A1 (en) * 2019-11-28 2021-06-03 The University Of Hong Kong Mesenchymal stromal cells as a reprogramming source for ipsc induction
WO2022266661A1 (en) * 2021-06-17 2022-12-22 Genentech, Inc. Automated cell culture system
US12385002B1 (en) * 2022-01-21 2025-08-12 Sahar M. H. Jaffal Automated primary cells' triturator
CN114606198A (zh) * 2022-03-25 2022-06-10 深圳临研医学有限公司 成骨发育不全诱导多能干细胞及其构建方法
WO2024020572A1 (en) 2022-07-22 2024-01-25 New York Stem Cell Foundation, Inc. Methods and compositions for the treatment of ptsd
AU2024208789A1 (en) * 2023-01-11 2025-07-24 Regeneron Pharmaceuticals, Inc. Systems and methods for a regulatory-compliant automated assay
WO2024228384A1 (ja) * 2023-04-30 2024-11-07 国立大学法人北海道大学 免疫寛容誘導用組成物
JP2025064611A (ja) * 2023-10-06 2025-04-17 住友化学株式会社 細胞チップ

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5994619A (en) 1996-04-01 1999-11-30 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of chimeric bovine or porcine animals using cultured inner cell mass cells
US5945577A (en) 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
US6114113A (en) 1997-08-11 2000-09-05 Chiron Corporation High efficiency genetic modification method
US8026096B1 (en) 1998-10-08 2011-09-27 Protein Sciences Corporation In vivo active erythropoietin produced in insect cells
US20110171185A1 (en) * 1999-06-30 2011-07-14 Klimanskaya Irina V Genetically intact induced pluripotent cells or transdifferentiated cells and methods for the production thereof
US6429013B1 (en) 1999-08-19 2002-08-06 Artecel Science, Inc. Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair
US6959438B2 (en) 2000-12-06 2005-10-25 Microsoft Corporation Interface and related methods for dynamically generating a filter graph in a development system
WO2003087292A2 (en) * 2002-04-08 2003-10-23 Millenium Biologix Inc. Automated tissue engineering system
EP1445320A1 (en) 2003-02-05 2004-08-11 ARTEMIS Pharmaceuticals GmbH Automated gene-targeting using non-toxic detectable markers
EP1743021B1 (en) * 2004-03-19 2014-03-26 Cytori Therapeutics, Inc. Method of preparing regenerative cells on a cell carrier and cell carrier containment device
US20070238175A1 (en) 2006-04-06 2007-10-11 Chi Alfred L Standardization of processes for culturing primary cells
GB0704406D0 (en) 2007-03-07 2007-04-18 Stem Cell Sciences Uk Ltd Automated culture of stem cells
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
US8815585B2 (en) 2007-06-29 2014-08-26 Cellular Dynamics International, Inc. Automated method and apparatus for embryonic stem cell culture
US20100196923A1 (en) * 2007-08-14 2010-08-05 Anthony Atala Pluripotent adult stem cells
US10047346B2 (en) 2008-08-08 2018-08-14 Mayo Foundation For Medical Education And Research Method of treating heart tissue using induced pluripotent stem cells
US9045737B2 (en) * 2008-12-13 2015-06-02 Dnamicroarray, Inc. Artificial three-dimensional microenvironment niche culture
BRPI0922572A2 (pt) * 2008-12-17 2019-09-24 Scripps Research Inst método para cultivar células pluripotentes, cultura de células de mamífero pluripotentes, meio de cultura de célula, célula animal pluripotente isolada, e, método para aumentar a pluripotência de uma célula de mamífero.
EP2398897B1 (en) * 2009-02-20 2017-06-28 Cellular Dynamics International, Inc. Methods and compositions for the differentiation of stem cells
KR101720961B1 (ko) 2009-02-27 2017-03-29 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 다능성 세포의 분화
CN101580816B (zh) 2009-04-23 2012-02-29 中国科学院广州生物医药与健康研究院 诱导多能性干细胞快速高效产生的新型无血清培养基以及使用其的方法
US20110020814A1 (en) * 2009-06-05 2011-01-27 Ipierian, Inc. Methods and compositions for selection of stem cells
DK2438160T3 (en) * 2009-06-05 2016-01-11 Cellular Dynamics Int Inc Reprogramming of T cells and hematopoietic cells
US20120252697A1 (en) 2009-09-01 2012-10-04 Mcmaster University Transformed human pluripotent stem cells and associated methods
WO2011037301A1 (ko) * 2009-09-22 2011-03-31 서울대학교병원 성체세포로부터 만능줄기세포를 유도하는 방법 및 그 방법에 의해 제조된 만능줄기세포
CA2796464C (en) 2010-04-16 2021-08-03 Immune Disease Institute, Inc. Sustained polypeptide expression from synthetic, modified rnas and uses thereof
US20110306516A1 (en) 2010-06-15 2011-12-15 The New York Stem Cell Foundation Methods for producing induced pluripotent stem cells
WO2012087965A2 (en) 2010-12-22 2012-06-28 Fate Therapauetics, Inc. Cell culture platform for single cell sorting and enhanced reprogramming of ipscs
EP2481795A1 (en) 2011-01-28 2012-08-01 Universität für Bodenkultur Wien Method of generating induced pluripotent stem cells and differentiated cells
WO2013082509A1 (en) * 2011-12-01 2013-06-06 The New York Stem Cell Foundation Automated system for producing induced pluripotent stem cells or differentiated cells

Also Published As

Publication number Publication date
KR20140101393A (ko) 2014-08-19
EP3653698A1 (en) 2020-05-20
CA2857295C (en) 2021-06-29
AU2012345676B2 (en) 2018-10-04
US10273459B2 (en) 2019-04-30
US20210332330A1 (en) 2021-10-28
JP6257520B2 (ja) 2018-01-10
US11732242B2 (en) 2023-08-22
CA3118842A1 (en) 2013-06-06
JP2015502747A (ja) 2015-01-29
BR122020005735B1 (pt) 2021-12-28
EP2785829A4 (en) 2015-04-29
CN110724636A (zh) 2020-01-24
ZA201803502B (en) 2022-12-21
IL289822B2 (en) 2023-06-01
IL232869A0 (en) 2014-07-31
KR20190124326A (ko) 2019-11-04
US20130345094A1 (en) 2013-12-26
US20240191203A1 (en) 2024-06-13
WO2013082509A1 (en) 2013-06-06
ES2773863T3 (es) 2020-07-15
KR20220141823A (ko) 2022-10-20
KR102039202B1 (ko) 2019-10-31
AU2021212081B2 (en) 2024-02-29
KR102631374B1 (ko) 2024-01-29
US10968435B2 (en) 2021-04-06
IL232869B (en) 2022-02-01
ES3038941T3 (en) 2025-10-16
KR102439238B1 (ko) 2022-08-31
ZA202205833B (en) 2024-09-25
AU2018286561A1 (en) 2019-01-24
BR112014013152A2 (pt) 2017-06-13
KR20210096700A (ko) 2021-08-05
IL289822A (en) 2022-03-01
CA2857295A1 (en) 2013-06-06
KR20240019378A (ko) 2024-02-14
CN104080906A (zh) 2014-10-01
AU2012345676A1 (en) 2014-06-19
US20190352613A1 (en) 2019-11-21
IL299477A (en) 2023-02-01
CA3118842C (en) 2023-12-12
IN2014CN04920A (https=) 2015-09-18
EP2785829A1 (en) 2014-10-08
EP3653698B1 (en) 2025-04-02
AU2024203546A1 (en) 2024-06-20
AU2021212081A1 (en) 2021-08-26
ZA201403945B (en) 2018-08-29
DK3653698T3 (da) 2025-07-07
BR112014013152B1 (pt) 2021-08-31
EP2785829B1 (en) 2019-10-16

Similar Documents

Publication Publication Date Title
DK2785829T3 (da) Automatiseret system til fremstilling af inducerede pluripotente stamceller eller differentierede celler
PT2718422T (pt) Meios de cultura para células estaminais
DK2893001T3 (da) Fremgangsmåde til dyrkning af mesenkymale stamceller
DK3543333T3 (da) Fremgangsmåde til screening af alfa-amylaser
EP2681306A4 (en) CELL CULTURE SYSTEM
DK3512197T3 (da) Fremgangsmåde til kortlægning af intraprædikationsmodus
DK3824991T3 (da) Fremgangsmåde til forberedelse af lithiumsulfat
PL2721708T3 (pl) Zautomatyzowany układ zarządzania energią w odpowiedzi na zapotrzebowanie
DK2775928T3 (da) Systemer og fremgangsmåder til forarbejdning af celler
DK2880151T3 (da) Fremgangsmåder til frembringelse af mesenkymale stemceller, som udskiller neurotrofiske faktorer
DK2882850T3 (da) System for preparation of adipose-derived stem cells
DK2658965T3 (da) Fremgangsmåde til fremstilling af inducerede pluripotente stamceller og differentierede celler
DK3050972T3 (da) Fremgangsmåder til fremstilling af eicosapentaensyre i thraustochytrider
DK2573571T3 (da) Sporbarhed til automatiseret farvningssystem
EP2739794A4 (en) AUTOMATIC WATER TUBES
DK2472055T3 (da) Værktøj til tilvejebringelse af kunstigt løft
DK3050961T3 (da) Fremgangsmåde til forbedring af effektiviteten ved inducering af pluripotente stamceller
GB201214704D0 (en) Stem cell culture method
DK3064573T3 (da) Fremgangsmåde til differentiering af pluripotent stamcelle induceret fra mesenkymal stamcelle til neuron
DK3064574T3 (da) Fremgangsmåde til differentiering af pluripotente stamceller induceret fra mesenkymstamceller til chondrocyt
PT2641833T (pt) Um método de ocultação solar
ZA201504388B (en) Method for culturing pluripotent stem cells
EP2800182A4 (en) FUEL CELL SYSTEM
DK2888591T3 (da) Fremgangsmåde til forbedret celleidentifikation
PL2742538T3 (pl) Sposób wytwarzania ogniwa słonecznego