DK2751261T3 - Dehydrogenasevarianter og polynukleotider kodende herfor - Google Patents

Dehydrogenasevarianter og polynukleotider kodende herfor Download PDF

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Publication number
DK2751261T3
DK2751261T3 DK12772176.9T DK12772176T DK2751261T3 DK 2751261 T3 DK2751261 T3 DK 2751261T3 DK 12772176 T DK12772176 T DK 12772176T DK 2751261 T3 DK2751261 T3 DK 2751261T3
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DK12772176.9T
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Monica Tassone
Maria Leonardo De
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Novozymes Inc
Novozymes As
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Publication of DK2751261T3 publication Critical patent/DK2751261T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01059—3-Hydroxypropionate dehydrogenase (1.1.1.59)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01276—Serine 3-dehydrogenase (1.1.1.276)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01298—3-Hydroxypropionate dehydrogenase (NADP+) (1.1.1.298)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (6)

1. Værtscelle, som omfatter en aktiv 3-hydroxypropionat-vej og et heterologt polynukleotid, der koder for en 3-hydroxypropionat-dehydrogenasevariant med 3-hydroxypropionat-dehydrogenaseaktivitet, hvor varianten omfatter eller består af SEQ ID NO: 10, 12, 20, 21,22, 23, 24, 25, 26, 27, 29, 30, 32 eller 81.
2. Værtscelle ifølge krav 1, hvor varianten har forhøjet specificitet for NAD(H) sammenlignet med NADP(H) (f.eks. større end 2 gange, 5 gange, 10 gange, 20 gange, 50 gange, 100 gange, 200 gange, 500 gange eller 1000-gange specificitet for NAD(H) sammenlignet med NADP(H)).
3. Værtscelle ifølge krav 1 eller 2, hvor cellen er en gærcelle {f.eks. en Issatchenkia orientalis-gærcelle).
4. Værtscelle ifølge ethvert af kravene 1-3, hvor cellen omfatter: PEP-carboxylaseaktivitet eller pyruvatcarboxylaseaktivitet; aspartat-aminotransferaseaktivitet; aspartat-decarboxylaseaktivitet; og beta-alanin/alpha-ketoglutarat-aminotransferase (BAAT)-aktivitet.
5. Værtscelle ifølge ethvert af kravene 1-4, hvor værtscellen omfatter et eller flere heterologe polynukleotider valgt fra: et heterologt polynukleotid, der koder for en PEP-carboxylase, et heterologt polynukleotid, der koder for en pyruvatcarboxylase, et heterologt polynukleotid, der koder for en aspartat-aminotransferase, et heterologt polynukleotid, der koder for en aspartat-decarboxylase, og et heterologt polynukleotid, der koder for en BAAT.
6. Fremgangsmåde til fremstilling af 3-hydroxypropionat, omfattende: a) dyrkning af værtscellen ifølge ethvert af kravene 1-5 under betingelser, der er befordrende for fremstilling af 3-hydroxypropionat; og b) indvinding af 3-hydroxypropionat. -1 -
DK12772176.9T 2011-09-30 2012-09-25 Dehydrogenasevarianter og polynukleotider kodende herfor DK2751261T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161541363P 2011-09-30 2011-09-30
PCT/US2012/057134 WO2013049073A1 (en) 2011-09-30 2012-09-25 Dehydrogenase variants and polynucleotides encoding same

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DK2751261T3 true DK2751261T3 (da) 2018-12-10

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US (3) US8728788B1 (da)
EP (1) EP2751261B1 (da)
JP (3) JP2014528726A (da)
CN (1) CN103857791B (da)
AU (1) AU2012316246B2 (da)
BR (1) BR112014007647A2 (da)
CA (1) CA2849303C (da)
DK (1) DK2751261T3 (da)
IN (1) IN2014CN03187A (da)
WO (1) WO2013049073A1 (da)

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US20180273915A1 (en) * 2014-12-19 2018-09-27 Novozymes A/S Recombinant Host Cells For The Production Of 3-Hydroxypropionic Acid
WO2016154046A2 (en) 2015-03-20 2016-09-29 Verdezyne, Inc. Biological methods for preparing 3-hydroxypropionic acid
WO2017035270A1 (en) 2015-08-24 2017-03-02 Novozymes A/S Beta-alanine aminotransferases for the production of 3-hydroxypropionic acid
WO2023168244A1 (en) * 2022-03-03 2023-09-07 Cargill, Incorporated Genetically modified yeast and fermentation processes for the production of 3-hydroxypropionate
WO2023168233A1 (en) * 2022-03-03 2023-09-07 Cargill, Incorporated Genetically modified yeast and fermentation processes for the production of 3-hydroxypropionate

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AU2012316246A1 (en) 2014-03-20
US8728788B1 (en) 2014-05-20
IN2014CN03187A (da) 2015-07-03
CN103857791A (zh) 2014-06-11
EP2751261A1 (en) 2014-07-09
EP2751261B1 (en) 2018-08-29
JP2019110916A (ja) 2019-07-11
US20160017296A1 (en) 2016-01-21
BR112014007647A2 (pt) 2017-04-18
CA2849303C (en) 2019-09-17
JP2014528726A (ja) 2014-10-30
JP2018033457A (ja) 2018-03-08
US20140212938A1 (en) 2014-07-31
CA2849303A1 (en) 2013-04-04
AU2012316246B2 (en) 2017-10-26
CN103857791B (zh) 2016-11-23
WO2013049073A1 (en) 2013-04-04
US9404091B2 (en) 2016-08-02
US9163220B2 (en) 2015-10-20

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