DK2748313T3 - Fremgangsmåder til hydrolyse af acetyleret celluloseholdigt materiale - Google Patents

Fremgangsmåder til hydrolyse af acetyleret celluloseholdigt materiale Download PDF

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DK2748313T3
DK2748313T3 DK12768989.1T DK12768989T DK2748313T3 DK 2748313 T3 DK2748313 T3 DK 2748313T3 DK 12768989 T DK12768989 T DK 12768989T DK 2748313 T3 DK2748313 T3 DK 2748313T3
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cellulosic material
ala
acetylated
acid
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Prashant Iyer
Armindo Ribeiro Gaspar
James Croonenberghs
Thomas P Binder
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Novozymes As
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/385—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Penicillium
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
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    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01072—Acetylxylan esterase (3.1.1.72)
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
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    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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    • Y02E50/10—Biofuels, e.g. bio-diesel

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Claims (9)

  1. Fremgangsmåder til hydrolyse af acetyleret celluloseholdigt materiale
    1. Fremgangsmåde til hydrolyse af acetyleret celluloseholdigt materiale, hvilken fremgangsmåde omfatter udsættelse af det acetylerede celluloseholdige materiale for et cellulolytisk præparat og en acetylxylanesterase (AXE), hvor nævnte acetylxylanesterase (AXE) stammer fra en Th/e/av/a-stamme og udviser mindst 80% identitet med SEQ ID NO: 1 heri.
  2. 2. Fremgangsmåde ifølge krav 1, hvor acetyleret celluloseholdigt materiale er forbehandlet celluloseholdigt materiale.
  3. 3. Fremgangsmåde ifølge krav 1 eller 2, hvor det acetylerede celluloseholdige materiale er plantemateriale, som er termomekanisk forarbejdet til pulp, såsom acetyleret pulp af majsblade og -stilke.
  4. 4. Fremgangsmåde ifølge et hvilket som helst af kravene 1-3, hvor nævnte acetylxylanesterase (AXE) stammer fra en stamme af slægten Thielavia, såsom en Thielavia terrestris-stamme, såsom en ifølge SEQ ID NO: 1 heri eller en acetylxylanesterase, som udviser mindst 85%, såsom mindst 90%, fortrinsvis mindst 95%, såsom mindst 96%, såsom mindst 97%, såsom mindst 98%, såsom mindst 99% identitet med SEQ ID NO: 1 heri.
  5. 5. Fremgangsmåde ifølge et hvilket som helst af kravene 1-4, hvor det cellulolytiske præparat er et cellulolytisk Trichoderma reesei-præparat, som endvidere omfatter Penicillium emerson/'/'-GH61 A-polypeptid med cellulolytisk forbedrende aktivitet, Aspergillus fumigatus-beta-glucosidasevariant og beta-xylosidase, som stammer fra en Aspergillus fumigatus- sta m m e.
  6. 6. Fremgangsmåde ifølge et hvilket som helst af kravene 1-5, hvor vægtforholdet mellem cellulolytisk præparat og acetylxylanesterase (AXE) er et forhold, som ligger i intervallet mellem 500:1 og 1:1, såsom mellem 50:1 og 2:1, såsom ca. 4:1.
  7. 7. Fremgangsmåde til fremstilling af et fermenteringsprodukt ud fra acetyleret celluloseholdigt materiale, hvilken fremgangsmåde omfatter: (a) hydrolyse af det acetylerede celluloseholdige materiale ifølge et hvilket som helst af kravene 1-6; (b) fermentering ved anvendelse af en fermenterende organisme og (c) eventuelt indvinding af fermenteringsproduktet.
  8. 8. Fremgangsmåde ifølge krav 7, hvor fermenteringsproduktet er ethanol.
  9. 9. Fremgangsmåde ifølge krav 7 eller 8, hvor det acetylerede celluloseholdige materiale er pulp af majsblade og -stilke.
DK12768989.1T 2011-09-23 2012-09-21 Fremgangsmåder til hydrolyse af acetyleret celluloseholdigt materiale DK2748313T3 (da)

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US201161538211P 2011-09-23 2011-09-23
PCT/US2012/056502 WO2013043981A1 (en) 2011-09-23 2012-09-21 Cellulolytic enzyme compositions and uses thereof

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EP (1) EP2748313B1 (da)
CN (2) CN108373498A (da)
BR (1) BR112014006853A2 (da)
CA (1) CA2849885A1 (da)
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WO (1) WO2013043981A1 (da)

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EP3344761A1 (en) * 2015-09-04 2018-07-11 Novozymes A/S Methods of inhibiting aa9 lytic polysaccharide monooxygenase catalyzed inactivation of enzyme compositions
CN108883400B (zh) 2016-02-19 2021-09-17 洲际大品牌有限责任公司 由生物质源形成多值料流的方法
US20190276809A1 (en) * 2016-11-24 2019-09-12 Dsm Ip Assets B.V. Enzyme composition
CN110965382A (zh) * 2019-12-11 2020-04-07 江西吉润花炮新材料科技有限公司 一种生物型竹纤维素提取方法

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EP2748313A1 (en) 2014-07-02
US20170342391A1 (en) 2017-11-30
BR112014006853A2 (pt) 2017-04-04
US20140234915A1 (en) 2014-08-21
WO2013043981A1 (en) 2013-03-28
EP2748313B1 (en) 2016-04-13
US9714416B2 (en) 2017-07-25
CN108373498A (zh) 2018-08-07
CA2849885A1 (en) 2013-03-28
CN104039958A (zh) 2014-09-10

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