DK2709445T3 - Dyr, der er resistente over for porcin reproduktions- og respirationssygdom - Google Patents
Dyr, der er resistente over for porcin reproduktions- og respirationssygdom Download PDFInfo
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- DK2709445T3 DK2709445T3 DK12785983.3T DK12785983T DK2709445T3 DK 2709445 T3 DK2709445 T3 DK 2709445T3 DK 12785983 T DK12785983 T DK 12785983T DK 2709445 T3 DK2709445 T3 DK 2709445T3
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Classifications
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- A—HUMAN NECESSITIES
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- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0276—Knock-out vertebrates
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/108—Swine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/7056—Lectin superfamily, e.g. CD23, CD72
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
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- C12N2800/00—Nucleic acids vectors
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (17)
1. Genmodificeret svin, hvori mindst én allel af et SIGLECl-gen er blevet inaktiveret.
2. Genmodificeret svin, hvori mindst én allel af et CD163-gen er blevet inaktiveret.
3. Genmodificeret svin ifølge krav 1 eller 2, hvori begge alleler af et SIGLECl-gen er blevet inaktiveret.
4. Genmodificeret svin ifølge et hvilket som helst af kravene 1-3, hvori begge alleler af et CD163-gen er blevet inaktiveret.
5. Genmodificeret svin, hvori mindst én allel af et SIGLECl-gen er blevet inaktiveret, og mindst én allel af et CD163-gen er blevet inaktiveret.
6. Fremgangsmåde til fremstilling af et genmodificeret svin, hvori mindst én allel af et SIGLECl-gen er blevet inaktiveret, hvilken fremgangsmåde omfatter: enukleation afen svine-oocyt; fusionering af oocytten med en donor-svine-fibroblastcelle, hvor fibroblastcellens genom omfatter mindst én inaktiveret SIGLECl-allel, og aktivering af oocytten til fremstilling af et embryo.
7. Fremgangsmåde til fremstilling af et genmodificeret svin, hvori mindst én allel af et CD163-gen er blevet inaktiveret, hvilken fremgangsmåde omfatter: enukleation afen svine-oocyt; fusionering af oocytten med en donor-svine-fibroblastcelle, hvor fibroblastcellens genom omfatter mindst én inaktiveret CD163-allel, og aktivering af oocytten til fremstilling af et embryo.
8. Fremgangsmåde ifølge krav 6 eller 7, hvor: den mindst ene allel er blevet inaktiveret ved partiel eller komplet deletion; den mindst ene allel er blevet inaktiveret ved anvendelse af et Cre-lox-rekombinationssystem, og/eller SIGLECl-allelen er blevet inaktiveret ved deletion af en del af exon 1 og hele exon 2 og 3.
9. Fremgangsmåde ifølge et hvilket som helst af kravene 6, 7 og 8, hvor: enukleationen udføres med en mikropipette i et mikromanipulationsmedium; fusioneringen udføres i et fusionsmedium, og/eller aktivering af oocytten omfatter inkubation af oocytten i nærvær af thimerosal.
10. Afkom af et genmodificeret svin fremstillet ved fremgangsmåden ifølge et hvilket som helst af kravene 6, 7, 8 og 9, hvor i afkommet mindst én allel af et SIGLECl-gen er inaktiveret; mindst én allel af et CD163-gen er inaktiveret; mindst én allel af et SIGLECl-gen og mindst én allel af et CD163-gen er inaktiveret; eller begge alleler af et SIGLECl-gen og begge alleler af et CD163 gen er inaktiveret.
11. Afkom af et genmodificeret svin fremstillet ved fremgangsmåden ifølge krav 7, hvor mindst én allel af et CD163-gen er inaktiveret i afkommet.
12. Svin ifølge et hvilket som helst af kravene 1-5, hvor den mindst ene allel er blevet inaktiveret ved partiel deletion.
13. Fremgangsmåde ifølge krav 6 eller 7, hvor den mindst ene allel er blevet inaktiveret ved partiel deletion.
14. Svin ifølge et hvilket som helst af kravene 1-5, hvor den mindst ene allel er blevet inaktiveret ved komplet deletion.
15. Fremgangsmåde ifølge krav 6 eller 7, hvor den mindst ene allel er blevet inaktiveret ved komplet deletion.
16. Svin ifølge et hvilket som helst af kravene 1-5, hvor den mindst ene allel er blevet inaktiveret ved anvendelse af et Cre-lox-rekombinationssystem.
17. Svin ifølge et hvilket som helst af kravene 1, 3 og 5, hvor SIGLECl-allelen er blevet inaktiveret ved deletion af en del af exon 1 og hele exon 2 og 3.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161519076P | 2011-05-16 | 2011-05-16 | |
| PCT/US2012/038193 WO2012158828A1 (en) | 2011-05-16 | 2012-05-16 | Porcine reproductive and respiratory syndrome virus resistant animals |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2709445T3 true DK2709445T3 (da) | 2018-11-19 |
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| DK12785983.3T DK2709445T3 (da) | 2011-05-16 | 2012-05-16 | Dyr, der er resistente over for porcin reproduktions- og respirationssygdom |
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| SI (1) | SI2709445T1 (da) |
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| CA2836288A1 (en) * | 2011-05-16 | 2012-11-22 | The Curators Of The University Of Missouri | Porcine reproductive and respiratory syndrome virus resistant animals |
| GB201313235D0 (en) | 2013-07-24 | 2013-09-04 | Univ Edinburgh | Antiviral Compositions Methods and Animals |
| US20150275231A1 (en) * | 2014-03-31 | 2015-10-01 | Mice With Horns, Llc | Method of Preventing or Reducing Virus Transmission in Animals |
| CN104593422A (zh) * | 2015-01-08 | 2015-05-06 | 中国农业大学 | 一种抗蓝耳病克隆猪的制备方法 |
| WO2016140353A1 (ja) * | 2015-03-04 | 2016-09-09 | 株式会社 医療実験用大動物供給事業準備会社 | 安定した表現型を示す疾患モデルブタおよびその作製方法 |
| AU2015404563B2 (en) * | 2015-08-06 | 2022-11-17 | The Curators Of The University Of Missouri | Pathogen-resistant animals having modified CD163 genes |
| US10091975B2 (en) * | 2015-08-06 | 2018-10-09 | The Curators Of The University Of Missouri | Pathogen-resistant animals having modified CD163 genes |
| GB201617559D0 (en) | 2016-10-17 | 2016-11-30 | University Court Of The University Of Edinburgh The | Swine comprising modified cd163 and associated methods |
| CN107937345B (zh) * | 2017-11-16 | 2019-01-29 | 山东蓝思种业股份有限公司 | 一种制备同时敲除cd163基因和cd13基因的猪成纤维细胞的方法 |
| US11160260B2 (en) | 2018-04-17 | 2021-11-02 | The Curators Of The University Of Missouri | Methods for protecting porcine fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV) |
| BR112020021066A2 (pt) * | 2018-04-17 | 2021-02-17 | The Curators Of The University Of Missouri | método para proteger um feto suíno da infecção com o vírus da síndrome respiratória e reprodutiva suína (prrsv) |
| CA3096283A1 (en) * | 2018-04-27 | 2019-10-31 | The Curators Of The University Of Missouri | Pathogen-resistant animals having modified aminopeptidase n (anpep) genes |
| CN109197780A (zh) * | 2018-11-26 | 2019-01-15 | 湖北天牧畜禽有限公司 | 一种减少仔猪生病的养殖方法 |
| WO2021024123A1 (en) * | 2019-08-06 | 2021-02-11 | Nzeno Limited | Donor pigs for xenotransplantation |
| CA3179710A1 (en) | 2020-05-05 | 2021-11-11 | Andrew Mark CIGAN | Methods for improving the health of porcine species by targeted inactivation of cd163 |
| EP4276109A1 (en) * | 2021-01-05 | 2023-11-15 | Public University Corporation Yokohama City University | Biomarker for determining fertility, and determining method using same |
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| WO1995016670A1 (en) | 1993-12-16 | 1995-06-22 | The Procter & Gamble Company | Process for making sulfonated fatty acid alkyl ester surfactant |
| GB9417831D0 (en) | 1994-09-05 | 1994-10-26 | Biotech & Biolog Scien Res | Biological manipulation |
| EP0833606B1 (en) | 1995-06-22 | 2003-10-01 | Minnesota Mining And Manufacturing Company | Stable hydroalcoholic compositions |
| US5683993A (en) | 1995-06-22 | 1997-11-04 | Ciba Vision Corporation | Compositions and methods for stabilizing polymers |
| GB9517779D0 (en) | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| GB9517780D0 (en) | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| JPH09298981A (ja) * | 1996-05-16 | 1997-11-25 | Y S New Technol Kenkyusho:Kk | トランスジェニックブタ |
| US5945577A (en) | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| US5995577A (en) | 1997-02-10 | 1999-11-30 | General Electric Company | Optimized steam vent locations for a nuclear fuel bundle |
| WO1998057538A1 (en) | 1997-06-18 | 1998-12-23 | The Curators Of The University Of Missouri | Complete activation of mammalian oocytes |
| US6740490B2 (en) * | 2001-01-29 | 2004-05-25 | Kansas State University Research Foundation | Identification and applications of porcine reproductive and respiratory syndrome virus host susceptibility factor(s) for improved swine breeding |
| CA2471035C (en) | 2001-12-21 | 2014-05-13 | The Curators Of The University Of Missouri | Knockout swine and methods for making the same |
| CA2559720A1 (en) * | 2004-03-17 | 2005-09-29 | Revivicor, Inc. | Tissue products derived from animals lacking any expression of functional alpha 1,3 galactosyltransferase |
| NZ550106A (en) | 2004-04-22 | 2009-06-26 | Kyowa Hakko Kirin Co Ltd | Transgenic animals and uses thereof |
| US7998485B2 (en) | 2006-05-11 | 2011-08-16 | Universiteit Gent | Sialoadhesin-related compositions and methods |
| US8563313B2 (en) * | 2007-02-13 | 2013-10-22 | Washington State University Research Foundation | Macrophage cell-lines for propagation of porcine reproductive and respiratory syndrome virus |
| US20100122356A1 (en) * | 2007-03-07 | 2010-05-13 | Aarhus Universitet | Pig model for psoriasis |
| US8912386B2 (en) | 2007-03-28 | 2014-12-16 | University Of Iowa Research Foundation | Transgenic pig model of cystic fibrosis |
| DK2191271T3 (da) * | 2007-07-27 | 2016-04-11 | Univ Gent | Permissive celler og anvendelser deraf |
| US20110016543A1 (en) * | 2008-12-04 | 2011-01-20 | Sigma-Aldrich Co. | Genomic editing of genes involved in inflammation |
| US20110016546A1 (en) * | 2008-12-04 | 2011-01-20 | Sigma-Aldrich Co. | Porcine genome editing with zinc finger nucleases |
| KR20180036810A (ko) * | 2009-08-14 | 2018-04-09 | 레비비코르 인코포레이션 | 당뇨병 치료를 위한 복수 형질전환 돼지 |
| CA2836288A1 (en) * | 2011-05-16 | 2012-11-22 | The Curators Of The University Of Missouri | Porcine reproductive and respiratory syndrome virus resistant animals |
| US10091975B2 (en) | 2015-08-06 | 2018-10-09 | The Curators Of The University Of Missouri | Pathogen-resistant animals having modified CD163 genes |
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