DK2668284T3 - Screening af mikrokoloni, der er indkapslet i gel - Google Patents

Screening af mikrokoloni, der er indkapslet i gel Download PDF

Info

Publication number
DK2668284T3
DK2668284T3 DK12705186.0T DK12705186T DK2668284T3 DK 2668284 T3 DK2668284 T3 DK 2668284T3 DK 12705186 T DK12705186 T DK 12705186T DK 2668284 T3 DK2668284 T3 DK 2668284T3
Authority
DK
Denmark
Prior art keywords
cell
hydrogel
cells
hydrogel particle
particle
Prior art date
Application number
DK12705186.0T
Other languages
English (en)
Inventor
Jeremy Agresti
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Application granted granted Critical
Publication of DK2668284T3 publication Critical patent/DK2668284T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (24)

1. Fremgangsmåde til detektering af en forbindelse, der er produceret af en rekombinant celle og ikke kan blandes med vand, hvilken fremgangsmåde omfatter: (a) at indkapsle cellen i en hydrogelpartikel; (b) at dyrke cellen i hydrogelpartiklen, hvorved der dannes en cellepopulation i partiklen; og (c) at detektere den rekombinant producerede forbindelse, der ikke kan blandes med vand, i hydrogelpartiklen, hvor detekteringen omfatter at bringe hydrogelpartiklen i kontakt med en fluorescerende solvatokrom farve og normalisere mængden af forbindelse, der ikke kan blandes med vand, i hydrogelpartiklen i forhold til mængden af cellebiomasse i hydrogelpartiklen.
2. Fremgangsmåde ifølge krav 1, hvor indkapslingen omfatter at bringe cellen i kontakt med en vandig hydrogelsuspension under betingelser, der er tilstrækkelige til dannelse af en hydrogelpartikel, der omfatter cellen.
3. Fremgangsmåde ifølge krav 2, hvor betingelserne omfatter at bringe den vandige hydrogelsuspension, der omfatter cellen, i kontakt med en fluorcar-bonolie, der omfatter et fluorineret overfladeaktivt stof.
4. Fremgangsmåde ifølge krav 3, hvor kontaktningen med en fluorcarbonolie omfatter at lade den vandige hydrogelsuspension, der omfatter cellen, på en mikrofluidindretning, der omfatter fluorcarbonolien, hvor hydrogelsuspensio-nen kommer i kontakt med fluorcarbonolien i en T-forgrening af mikrofluidind-retningen, hvor kontaktningen med fluorcarbonolie resulterer i dannelse af en ikke-vandig hydrogelpartikel, der omfatter cellen.
5. Fremgangsmåde ifølge krav 4, endvidere omfattende trinnet med at separere hydrogelpartiklen fra fluorcarbonolien.
6. Fremgangsmåde ifølge krav 1, endvidere omfattende trinnet med at dyrke cellen i hydrogelpartiklen før detekteringen.
7. Fremgangsmåde ifølge krav 6, hvor dyrkningen er i et tidsrum på 12 til 24 timer.
8. Fremgangsmåde til screening af et bibliotek af celler for en celle, der re-kombinant producerer en forbindelse, der ikke kan blandes med vand, hvilken fremgangsmåde omfatter: (a) at indkapsle hver celle i biblioteket i en hydrogelpartikel; (b) at dyrke cellen i hydrogelpartiklen, hvorved der dannes en cellepopulation i partiklen; (c) at detektere den rekombinant producerede forbindelse, der ikke kan blandes med vand, i hver hydrogelpartikel, hvor detekteringen omfatter at bringe hydrogelpartiklen i kontakt med en fluorescerende solvatokrom farve og normalisere mængden af forbindelse, der ikke kan blandes med vand, i hydrogelpartiklen i forhold til mængden af cellebiomasse i hydrogelpartiklen; og (d) at vælge en celle, der producerer den rekombinant producerede forbindelse, der ikke kan blandes med vand.
9. Fremgangsmåde til at berige en population af celler for celler, der rekombinant producerer en forbindelse, der ikke kan blandes med vand, hvilken fremgangsmåde omfatter: (a) at tilvejebringe en population af hydrogelpartikler, hvor populationen omfatter hydrogelpartikler, der indkapsler en celle eller en klonal population af celler, der er genmodificeret til at producere en forbindelse, der ikke kan blandes med vand; (b) at dyrke cellen eller den klonale population af celler i hver hydrogelpartikel; (c) at detektere en hydrogelpartikel, der omfatter den rekombinant producerede forbindelse, der ikke kan blandes med vand, hvor detekteringen omfatter at bringe hydrogelpartiklen i kontakt med en fluorescerende solvatokrom farve og normalisere mængden af forbindelse, der ikke kan blandes med vand, i hydrogelpartiklen i forhold til mængden af cellebiomasse i hydrogelpartiklen; (d) at udvinde cellen eller den klonale population af celler fra hydrogelpartiklen af trin (b); (e) at genindkapsle cellen eller den klonale population af celler fra trin (c); og (f) at gentage trin (a) - (d).
10. Hydrogelindkapslet celle eller klonal cellepopulation, der omfatter en re-kombinant produceret forbindelse, der ikke kan blandes med vand, og en fluorescerende solvatokrom farve, der muliggør normalisering af mængden af forbindelse, der ikke kan blandes med vand, i hydrogelen i forhold til mængden af cellebiomasse i hydrogelen.
11. Hydrogelpartikel, der omfatter en celle eller klonal cellepopulation, en rekombinant produceret forbindelse, der ikke kan blandes med vand, og en fluorescerende solvatokrom farve, der muliggør normalisering af mængden af forbindelse, der ikke kan blandes med vand, i hydrogelen i forhold til mængden af cellebiomasse i hydrogelen.
12. Fremgangsmåde ifølge krav 1, hydrogelindkapslet celle eller klonal cellepopulation ifølge krav 10 eller hydrogelpartikel ifølge krav 11, hvor den fluorescerende solvatokrome farve er Nile Red.
13. Fremgangsmåde ifølge krav 1, hydrogelindkapslet celle eller klonal cellepopulation ifølge krav 10, eller hydrogelpartikel ifølge krav 11, hvor cellen er vælges fra gruppen bestående af en gærcelle, en bakteriecelle, en pattedyrscelle, en svampecelle, en insektcelle og en plantecelle.
14. Fremgangsmåde ifølge krav 1, hydrogelindkapslet celle eller klonal cellepopulation ifølge krav 10 eller hydrogelpartikel ifølge krav 11, hvor cellen er en gærcelle.
15. Fremgangsmåde ifølge krav 1, hydrogelindkapslet celle eller klonal cellepopulation ifølge krav 10 eller hydrogelpartikel ifølge krav 11, hvor den rekombinant producerede forbindelse, der ikke kan blandes med vand, vælges fra gruppen bestående af en isoprenoid, et polyketid og en fedtsyre.
16. Fremgangsmåde ifølge krav 1 eller hydrogelpartikel ifølge krav 11, hvor hydrogelpartiklen kan fastholde en forbindelse, der ikke kan blandes med vand.
17. Fremgangsmåde ifølge krav 1 eller hydrogelpartikel ifølge krav 11, hvor hydrogelpartiklen omfatter agarose.
18. Fremgangsmåde ifølge krav 1 eller hydrogelpartikel ifølge krav 11, hvor hydrogelpartiklen er mindre end ca. 1 millimeter i diameter.
19. Fremgangsmåde ifølge krav 1 eller hydrogelpartikel ifølge krav 11, hvor hydrogelpartiklen er mindre end ca. 500 mikrometer i diameter.
20. Fremgangsmåde ifølge krav 1 eller hydrogelpartikel ifølge krav 11, hvor hydrogelpartiklen er mindre end ca. 100 mikrometer i diameter.
21. Fremgangsmåde ifølge krav 1 eller hydrogelpartikel ifølge krav 11, hvor hydrogelpartiklen er mindre end ca. 50 mikrometer i diameter.
22. Fremgangsmåde ifølge krav 1 eller hydrogelpartikel ifølge krav 11, hvor hydrogelpartiklen er ca. 50, 45, 40, 35, 30 eller 25 mikrometer i diameter.
23. Fremgangsmåde ifølge krav 15, hydrogelindkapslet celle eller klonal cellepopulation ifølge krav 15 eller hydrogelpartikel ifølge krav 15, hvor isopre-noidet er et C5-C30-isoprenoid.
24. Fremgangsmåde ifølge krav 15, hydrogelindkapslet celle eller klonal cellepopulation ifølge krav 15 eller hydrogelpartikel ifølge krav 15, hvor isopre-noidet vælges fra gruppen bestående af abietadien, amorphadien, caren, a-farnesen, β-farnesen, farnesol, geraniol, geranylgeraniol, isopren, linalool, limonen, myrcen, nerolidol, ocimen, patchoulol, β-pinen, sabinen, y-terpinen, terpinolen og valencen.
DK12705186.0T 2011-01-28 2012-01-27 Screening af mikrokoloni, der er indkapslet i gel DK2668284T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161437214P 2011-01-28 2011-01-28
US201161486211P 2011-05-13 2011-05-13
PCT/US2012/023024 WO2012103516A1 (en) 2011-01-28 2012-01-27 Gel-encapsulated microcolony screening

Publications (1)

Publication Number Publication Date
DK2668284T3 true DK2668284T3 (da) 2014-12-15

Family

ID=45722697

Family Applications (1)

Application Number Title Priority Date Filing Date
DK12705186.0T DK2668284T3 (da) 2011-01-28 2012-01-27 Screening af mikrokoloni, der er indkapslet i gel

Country Status (14)

Country Link
US (1) US20120196770A1 (da)
EP (1) EP2668284B1 (da)
JP (1) JP2014508516A (da)
KR (1) KR20140012072A (da)
CN (1) CN103459610A (da)
AU (1) AU2012211052A1 (da)
BR (1) BR112013017884A2 (da)
CA (1) CA2824420A1 (da)
DK (1) DK2668284T3 (da)
HK (1) HK1186498A1 (da)
MX (1) MX2013008062A (da)
SG (1) SG192107A1 (da)
WO (1) WO2012103516A1 (da)
ZA (1) ZA201304895B (da)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150177115A1 (en) 2012-04-06 2015-06-25 Slingshot Biosciences Hydrogel particles with tunable optical properties
KR101663556B1 (ko) * 2014-06-13 2016-10-07 한국생명공학연구원 세포를 함유하는 오일 내 하이드로겔 에멀젼, 세포외 구획화 방법 및 신규효소 초고속 스크리닝법
US10538732B2 (en) 2014-10-31 2020-01-21 National University Corporation Tokyo University Of Agriculture And Technology Cell isolation method and cell trapping filter
DE102015106870B3 (de) 2014-11-10 2016-02-25 Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut System zur Inkubation von mikrofluidischen Tropfen und Verfahren zur Bereitstellung von homogenen Inkubationsbedingungen in einer Tropfeninkubationseinheit
KR20230141834A (ko) * 2015-02-09 2023-10-10 슬링샷 바이오사이언시즈 인코포레이티드 튜닝가능한 광 특성을 갖는 하이드로겔 입자 및 이를 사용하기 위한 방법
US11046828B2 (en) * 2015-04-21 2021-06-29 Trustees Of Tufts College Fabrication of macroporous monodisperse hydrogel microspheres
JP6728238B2 (ja) * 2015-05-19 2020-07-22 バイオ−ラッド・イノヴァシオン 試料中の分析物を検出するためのデバイスにおける低密度不混和性化合物の使用
US10233487B2 (en) * 2016-03-24 2019-03-19 Bio-Rad Laboratories, Inc. Use of gel beads to control droplet dispersion
CN105907601B (zh) * 2016-05-12 2018-08-21 大连理工大学 一种基于固态双凝胶的微生物三维群落结构分析芯片及应用
WO2018017845A1 (en) * 2016-07-21 2018-01-25 Massachusetts Intitute Of Technology Materials and devices containing hydrogel-encapsulated cells
EP3421131A1 (en) * 2017-06-30 2019-01-02 Blink AG A sample cartridge for incubating and/or analyzing a dispersion of particles, cells or droplets
KR102187682B1 (ko) * 2018-06-08 2020-12-07 한국과학기술원 타입 iii 폴리케타이드 합성 효소 기반 신규 말로닐-코에이 바이오센서 및 그 용도
US11154863B2 (en) 2018-10-08 2021-10-26 Bioelectronica Corporation Systems and methods for optically processing samples
TW202141019A (zh) 2020-01-24 2021-11-01 美商史林夏特生化公司 類細胞校正粒子之組合物與方法
CN115485556A (zh) 2020-05-04 2022-12-16 弹弓生物科学公司 用于多路复用测定的被动光学条形码化的组合物和方法
CN111650898B (zh) * 2020-05-13 2023-10-20 大唐七台河发电有限责任公司 具备高容错性能的集散控制系统及方法
BR112023020542A2 (pt) * 2021-04-05 2023-12-05 Ab Mauri Cepa de levedura saccharomyces, derivada de uma cepa de levedura saccharomyces, métodos de produção da derivada de uma cepa de levedura saccharomyces, da levedura mutante, da levedura evoluída e de um produto de fermentação a partir de um substrato, leveduras mutante, evoluída, geneticamente modificada e recombinante de uma cepa de levedura, processos para a produção de etanóis a partir de um substrato e de uma biomassa, e, composição
CN112941007B (zh) * 2021-04-19 2022-06-17 广西大学 一种香蕉枯萎病菌的单孢分离方法
KR20230106910A (ko) * 2022-01-07 2023-07-14 인하대학교 산학협력단 비색형 바이오센서, 이의 제조방법 및 이를 이용한 항생제 감수성 검사방법

Family Cites Families (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3477936D1 (en) 1983-03-08 1989-06-01 Merck & Co Inc Recombinant dna cloning vector pve1, deletion and hybrid mutants, and recombinant derivatives thereof, products and processes
US4703009A (en) 1983-03-08 1987-10-27 Merck & Co., Inc. RDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes
US5272065A (en) 1983-10-20 1993-12-21 Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
SE8405105L (sv) * 1984-05-24 1985-11-25 Damon Biotech Inc Sett for screening av celler
US4801529A (en) * 1985-06-18 1989-01-31 Brandeis University Methods for isolating mutant microoganisms using microcapsules coated with indicator material
IL81892A (en) 1986-03-21 1995-10-31 Lilly Co Eli Recombinant AND molecule containing a sequence encoding O-methyltransfer macrocin
US5672497A (en) 1986-03-21 1997-09-30 Eli Lilly And Company Method for increasing the antibiotic-producing ability of antibiotic-producing microorganisms
US5098837A (en) 1988-06-07 1992-03-24 Eli Lilly And Company Macrolide biosynthetic genes for use in streptomyces and other organisms
US5149638A (en) 1988-09-29 1992-09-22 Eli Lilly And Company Tylosin biosynthetic genes tylA, tylB and tylI
US5252474A (en) 1989-03-31 1993-10-12 Merck & Co., Inc. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use
US5523162A (en) 1990-04-03 1996-06-04 Ppg Industries, Inc. Water repellent surface treatment for plastic and coated plastic substrates
US5824513A (en) 1991-01-17 1998-10-20 Abbott Laboratories Recombinant DNA method for producing erythromycin analogs
WO1993013663A1 (en) 1992-01-17 1993-07-22 Abbott Laboratories Method of directing biosynthesis of specific polyketides
US6060234A (en) 1991-01-17 2000-05-09 Abbott Laboratories Polyketide derivatives and recombinant methods for making same
US5658767A (en) * 1991-01-24 1997-08-19 Martek Corporation Arachidonic acid and methods for the production and use thereof
WO1995012661A1 (en) 1993-11-02 1995-05-11 Merck & Co., Inc. Dna encoding triol polyketide synthase
US5716849A (en) 1994-06-08 1998-02-10 Novartis Finance Corporation Genes for the biosynthesis of soraphen
DE4426396A1 (de) * 1994-07-26 1996-02-01 Ulrich Prof Dr Zimmermann Verfahren zur Herstellung konzentrierter Lösungen von mikroverkapselten Zellen oder von suspendierten Wirkstoffen in mikroverkapselter Form
CA2197160C (en) 1996-02-22 2007-05-01 Stanley Gene Burgett Platenolide synthase gene
CA2197524A1 (en) 1996-02-22 1997-08-22 Bradley Stuart Dehoff Polyketide synthase genes
DE69736673T2 (de) 1996-08-20 2007-09-13 Novartis Ag Rifamycin biosynthese genkluster
AU6571598A (en) * 1997-03-18 1998-10-12 Chromaxome Corporation Methods for screening compounds using encapsulated cells
EP1032679A2 (fr) 1997-07-25 2000-09-06 Hoechst Marion Roussel Genes de biosynthese et de transfert des 6-desoxyhexoses chez saccharopolyspora erythraea et chez streptomyces antibioticus et leur utilisation
US6090601A (en) 1998-01-23 2000-07-18 Kosan Bioscience Sorangium polyketide synthase
US6143526A (en) 1998-03-09 2000-11-07 Baltz; Richard H. Biosynthetic genes for spinosyn insecticide production
NZ509006A (en) 1998-05-28 2003-09-26 Kosan Biosciences Inc Recombinant narbonolide polyketide synthase
NZ508326A (en) 1998-06-18 2003-10-31 Novartis Ag A polyketide synthase and non ribosomal peptide synthase genes, isolated from a myxobacterium, necessary for synthesis of epothiones A and B
US6265202B1 (en) 1998-06-26 2001-07-24 Regents Of The University Of Minnesota DNA encoding methymycin and pikromycin
US6485703B1 (en) * 1998-07-31 2002-11-26 The Texas A&M University System Compositions and methods for analyte detection
ATE346938T1 (de) 1998-10-02 2006-12-15 Kosan Biosciences Inc Polyketid synthase enzyme und rekombinante dna konstrukte dafür, zur herstellung von mit fk-506 und fk-520 verwandten verbindungen
EP1124969A2 (en) 1998-10-29 2001-08-22 Kosan Biosciences, Inc. Recombinant oleandolide polyketide synthase
AU768220B2 (en) 1998-11-20 2003-12-04 Kosan Biosciences, Inc. Recombinant methods and materials for producing epothilone and epothilone derivatives
JP4257958B2 (ja) * 1999-01-07 2009-04-30 味の素株式会社 微生物の単離方法
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
US7192751B2 (en) 2001-12-06 2007-03-20 The Regents Of The University Of California Biosynthesis of amorpha-4,11-diene
CN101080497B (zh) * 2003-12-16 2010-12-29 金伯利-克拉克环球有限公司 微生物检测与定量
WO2005072334A2 (en) * 2004-01-23 2005-08-11 Arizona Board Of Regents For And On Behalf Of Arizona State University Photoresponsive hydrogels
EP2371967B1 (en) * 2005-03-18 2015-06-03 DSM IP Assets B.V. Production of carotenoids in oleaginous yeast and fungi
WO2006122147A2 (en) * 2005-05-10 2006-11-16 Rutgers, The State University Of New Jersey Alginate poly-l-lysine encapsulation as a technology for controlled differentiation of embryonic stem cells
US7846222B2 (en) * 2006-10-10 2010-12-07 Amyris Biotechnologies, Inc. Fuel compositions comprising farnesane and farnesane derivatives and method of making and using same
US20080102276A1 (en) * 2006-10-27 2008-05-01 Ali Khademhosseini Controlled Release of a Curing Agent for the Generation of Microstructures
US20100227767A1 (en) * 2007-07-26 2010-09-09 Boedicker James Q Stochastic confinement to detect, manipulate, and utilize molecules and organisms
CA2700211C (en) * 2007-09-20 2019-07-30 Amyris Biotechnologies, Inc. Production of isoprenoids
US20110190165A1 (en) * 2008-05-16 2011-08-04 Randall Weselake High throughput methods of identifying neutral lipid synthases
ES2464740T3 (es) 2009-06-01 2014-06-03 Amyris, Inc. Método para generar un microbio modificado genéticamente
BRPI1013896A2 (pt) 2009-06-01 2015-08-25 Amyris Inc Célula de levedura, e, métodos para produzir um composto isoprenóide e para detectar em uma amostra biológica a presença ou ausência de uma célula microbiana geneticamente.
CN101762572B (zh) * 2010-01-28 2011-04-20 北京交通大学 用于pH值传感的比率荧光纳米水凝胶及其制备方法

Also Published As

Publication number Publication date
JP2014508516A (ja) 2014-04-10
SG192107A1 (en) 2013-08-30
MX2013008062A (es) 2013-08-09
BR112013017884A2 (pt) 2019-09-24
HK1186498A1 (en) 2014-03-14
KR20140012072A (ko) 2014-01-29
WO2012103516A1 (en) 2012-08-02
ZA201304895B (en) 2014-09-25
CN103459610A (zh) 2013-12-18
AU2012211052A1 (en) 2013-09-05
EP2668284A1 (en) 2013-12-04
CA2824420A1 (en) 2012-08-02
EP2668284B1 (en) 2014-10-15
US20120196770A1 (en) 2012-08-02

Similar Documents

Publication Publication Date Title
DK2668284T3 (da) Screening af mikrokoloni, der er indkapslet i gel
US11230716B2 (en) Methods for stabilizing production of acetyl-coenzyme A derived compounds
US20210155939A1 (en) Methods for stabilizing production of acetyl-coenzyme a derived compounds
JP5989098B2 (ja) アセチル−補酵素ａ誘導化合物の産生
US20140057314A1 (en) Methods and compositions for detecting microbial production of water-immiscible compounds
WO2012016172A2 (en) Genetically modified microbes producing increased levels of acetyl-coa derived compounds
AU2012256195A1 (en) Methods and compositions for detecting microbial production of water-immiscible compounds