DK2634195T3 - Separation af levende uberørte neuroner - Google Patents
Separation af levende uberørte neuroner Download PDFInfo
- Publication number
- DK2634195T3 DK2634195T3 DK12157671.4T DK12157671T DK2634195T3 DK 2634195 T3 DK2634195 T3 DK 2634195T3 DK 12157671 T DK12157671 T DK 12157671T DK 2634195 T3 DK2634195 T3 DK 2634195T3
- Authority
- DK
- Denmark
- Prior art keywords
- cells
- antigen
- specific
- neuronal
- antibody
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0619—Neurons
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2839—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily
- C07K16/2848—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily against integrin beta3-subunit-containing molecules, e.g. CD41, CD51, CD61
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
Claims (15)
1) Anvendelse af celleoverflademarkøren CD51 som en negativ selektionsmarkør for neuronale celler.
2) Fremgangsmåde til berigelse, isolation og/eller detektering af neuronale celler, der omfatter trinnene a) etablering af kontakt mellem en prøve, der indeholder neuronale celler, og et antigen-bindende fragment, der er specifikt for CD51 -antigenet koblet til en fast fase, hvorved prøvens CD51 positive celler mærkes, b) isolering af prøvens ikke-mærkede celler.
3) Fremgangsmåde ifølge krav 2, hvor prøven i trin a) samtidig bringes i kontakt med et antigenbindende fragment, der er specifikt for CD51 -antigenet koblet til en fast fase og med et antigen-bindende fragment, der er specifikt for en astrocytspecifik celleoverflademarkør koblet til en fast overflade, hvorved prøvens CD51 positive celler og de celler, der udtrykker en astrocytspecifik overflademarkør, mærkes.
4) Fremgangsmåde ifølge krav 2, hvor fremgangsmåden omfatter følgende supplerende trin efter trin a) og b): c) etablering af kontakt mellem de ikke-mærkede celler fra trin b) og et antigen-bindende fragment, der er specifikt for en astrocytspecifik celleoverflademarkør koblet til en fast overflade, hvorved de celler, der udtrykker en astrocytspecifik overflademarkør, mærkes, d) isolering af de ikke-mærkede celler.
5) Fremgangsmåde ifølge et hvilket som helst af kravene 2 til 4, hvor de antigenbindende fragmenter er antistoffer eller antistoffragmenter.
6) Fremgangsmåde ifølge et hvilket som helst af kravene 2 til 5, hvor den faste fase er en magnetisk partikel.
7) Fremgangsmåde ifølge et hvilket som helst af kravene 2 til 6, hvor det antigenbindende fragment, der er specifikt for en astrocytspecifik celleoverflademarkør, er udvalgt fra gruppen bestående af Anti-ACSA-2, Anti-GLAST (ACSA-1).
8) Fremgangsmåde ifølge et hvilket som helst af kravene 2 til 7, hvor det antigenbindende fragment, der er specifikt for en astrocytspecifik celleoverflademarkør, er ACSA-2.
9) Fremgangsmåde ifølge et hvilket som helst af kravene 2 til 8, hvor de neuronale celler er pattedyreceller.
10) Fremgangsmåde ifølge krav 9, hvor pattedyrecelleme er muse- eller humane celler.
11) Neuronalcellesammensætning, der kan opnås ved fremgangsmåden ifølge et hvilket som helst af kravene 2 til 10, hvor neuronalcellesammensætningen indeholder mindst 80 % CD51 negative neuronale celler.
12) Farmaceutisk sammensætning omfattende en neuronalcellesammensætning, der kan opnås ved fremgangsmåden ifølge et hvilket som helst af kravene 2 til 10, hvor neuronalcellesammensætningen indeholder mindst 80 % CD51 negative neuronale celler.
13) Kit til berigelse, isolation og/eller detektering af neuronale celler omfattende a) et anti-CD51 -antistof eller antistoffragment deraf koblet til en fast fase; og, b) et antistof eller antistoffragment deraf, der er specifikt for en astrocytspecifik celleoverflademarkør koblet til en fast fase.
14) Kit ifølge krav 13, hvor den faste fase er en magnetisk partikel.
15) Kit ifølge krav 13 eller 14, hvor antistoffet eller antistofffagmentet deraf, der er specifikt for en astrocytspecifik celleoverflademarkør, er et Anti-ACSA-2- eller Anti-GLAST- (ACSA-1) antistof eller antistoffragment deraf.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12157671.4A EP2634195B1 (en) | 2012-03-01 | 2012-03-01 | Separation of living untouched neurons |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2634195T3 true DK2634195T3 (da) | 2017-02-27 |
Family
ID=45774070
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12157671.4T DK2634195T3 (da) | 2012-03-01 | 2012-03-01 | Separation af levende uberørte neuroner |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9080149B2 (da) |
| EP (1) | EP2634195B1 (da) |
| CN (1) | CN103289955B (da) |
| CA (1) | CA2801350A1 (da) |
| DK (1) | DK2634195T3 (da) |
| ES (1) | ES2616289T3 (da) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3097187B1 (en) * | 2014-01-21 | 2018-07-04 | Stemcell Technologies Inc. | Method for separating target entities from a sample using a composition of mono-specific tetrameric antibody complexes coupled to a surface |
| CN104977414B (zh) * | 2014-04-09 | 2016-09-28 | 上海交通大学医学院 | 鉴定骨髓中是否存在保护白血病干细胞微环境的试剂盒及其应用 |
| US20170191032A1 (en) * | 2014-05-06 | 2017-07-06 | The Board Of Regents Of The University Of Texas System | Identification and isolation of neural stem cells and neurosphere initiating cells |
| EP3184548A1 (en) * | 2015-12-23 | 2017-06-28 | Miltenyi Biotec GmbH | Chimeric antigen receptor with cytokine receptor activating or blocking domain |
| CN115667491A (zh) | 2020-03-10 | 2023-01-31 | 赛阿瑞斯公司 | 用于细胞处理的系统、装置及方法 |
| WO2022266431A1 (en) * | 2021-06-18 | 2022-12-22 | The Texas A&M University System | Methods of processing adult neural cells from mammals and assays thereof |
| CN114276993A (zh) * | 2021-11-11 | 2022-04-05 | 上海昆盟生物科技有限公司 | 一种用于分离大鼠下丘脑弓状核神经元的方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE68919715T2 (de) | 1988-12-28 | 1995-04-06 | Stefan Miltenyi | Verfahren sowie materialien zur hochgraduierten magnetischen abspaltung biologischer materialien. |
| US6020210A (en) | 1988-12-28 | 2000-02-01 | Miltenvi Biotech Gmbh | Methods and materials for high gradient magnetic separation of biological materials |
| GB2414995B (en) * | 2004-06-09 | 2006-11-15 | Univ Edinburgh | Neural stem cells |
| ES2313805B1 (es) * | 2004-10-04 | 2009-12-23 | Cellerix, S.L. | Identificacion y aislamiento de celulas multipotentes de tejido mesenquimal no osteocondral. |
| US20080213232A1 (en) * | 2004-10-25 | 2008-09-04 | Rutgers, The State University Of New Jersey | Radial Glial Cells Promote Nerve Regeneration and Functional Recovery Following Spinal Cord Injury |
| EP2307050A4 (en) * | 2008-07-04 | 2012-07-25 | Ono Pharmaceutical Co | USE OF A EFFECTIVENESS MARKER TO OPTIMIZE THE THERAPEUTIC EFFECTIVENESS OF AN ANTI-HUMAN PD-1 ANTIBODY ON CANCERS |
-
2012
- 2012-03-01 EP EP12157671.4A patent/EP2634195B1/en active Active
- 2012-03-01 ES ES12157671.4T patent/ES2616289T3/es active Active
- 2012-03-01 DK DK12157671.4T patent/DK2634195T3/da active
-
2013
- 2013-01-09 CA CA 2801350 patent/CA2801350A1/en not_active Abandoned
- 2013-02-27 CN CN201310067771.8A patent/CN103289955B/zh active Active
- 2013-02-27 US US13/779,660 patent/US9080149B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| ES2616289T3 (es) | 2017-06-12 |
| CA2801350A1 (en) | 2013-09-01 |
| US9080149B2 (en) | 2015-07-14 |
| EP2634195B1 (en) | 2017-02-01 |
| EP2634195A1 (en) | 2013-09-04 |
| US20130316373A1 (en) | 2013-11-28 |
| CN103289955A (zh) | 2013-09-11 |
| CN103289955B (zh) | 2017-10-24 |
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