DK2500436T3 - A method, probe and kit for in situ hybridization of DNA and use thereof - Google Patents

A method, probe and kit for in situ hybridization of DNA and use thereof Download PDF

Info

Publication number
DK2500436T3
DK2500436T3 DK11290139.2T DK11290139T DK2500436T3 DK 2500436 T3 DK2500436 T3 DK 2500436T3 DK 11290139 T DK11290139 T DK 11290139T DK 2500436 T3 DK2500436 T3 DK 2500436T3
Authority
DK
Denmark
Prior art keywords
mitochondrial
dna
probe
nucleic acid
seq
Prior art date
Application number
DK11290139.2T
Other languages
Danish (da)
English (en)
Inventor
Laurent Arnaud Chatre
Miria Ricchetti
Original Assignee
Pasteur Institut
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut, Centre Nat Rech Scient filed Critical Pasteur Institut
Application granted granted Critical
Publication of DK2500436T3 publication Critical patent/DK2500436T3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK11290139.2T 2011-03-17 2011-03-17 A method, probe and kit for in situ hybridization of DNA and use thereof DK2500436T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP11290139.2A EP2500436B1 (en) 2011-03-17 2011-03-17 Method, probe and kit for DNA in situ hybridation and use thereof

Publications (1)

Publication Number Publication Date
DK2500436T3 true DK2500436T3 (en) 2016-08-29

Family

ID=45833435

Family Applications (1)

Application Number Title Priority Date Filing Date
DK11290139.2T DK2500436T3 (en) 2011-03-17 2011-03-17 A method, probe and kit for in situ hybridization of DNA and use thereof

Country Status (5)

Country Link
US (2) US9957555B2 (enExample)
EP (2) EP2500436B1 (enExample)
JP (1) JP6262533B2 (enExample)
DK (1) DK2500436T3 (enExample)
WO (1) WO2012123588A1 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3604555B1 (en) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequencing by structure assembly
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
EP4491741B1 (en) 2011-12-22 2025-10-15 President And Fellows Of Harvard College Compositions and methods for analyte detection
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
SG10201710030QA (en) 2013-06-04 2018-01-30 Harvard College Rna-guided transcriptional regulation
US10179932B2 (en) 2014-07-11 2019-01-15 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
WO2017079406A1 (en) * 2015-11-03 2017-05-11 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
WO2017100343A1 (en) 2015-12-07 2017-06-15 Arc Bio, Llc Methods and compositions for the making and using of guide nucleic acids
AU2017257624B2 (en) 2016-04-25 2023-05-25 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
JP7239465B2 (ja) 2016-08-31 2023-03-14 プレジデント アンド フェローズ オブ ハーバード カレッジ 蛍光in situ配列決定による検出のための核酸配列ライブラリの作製法
US11185568B2 (en) 2017-04-14 2021-11-30 President And Fellows Of Harvard College Methods for generation of cell-derived microfilament network
EP4741512A2 (en) 2017-09-22 2026-05-13 University of Washington In situ combinatorial labeling of cellular molecules
EP3836967A4 (en) 2018-07-30 2022-06-15 ReadCoor, LLC METHODS AND SYSTEMS FOR SAMPLE PROCESSING OR ANALYSIS
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
CN115362264A (zh) 2020-01-29 2022-11-18 10X基因组学有限公司 用于分析物检测的组合物和方法
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
CN114891861B (zh) * 2022-05-05 2024-07-26 江苏省食品药品监督检验研究院 一种检测抗体中cho细胞残留dna的raa试剂盒及其检测方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
WO2000061819A2 (en) * 1999-04-14 2000-10-19 California Institute Of Technology Methods of detection and modulation of age-related mutations
US20070190534A1 (en) 2001-06-11 2007-08-16 Genesis Genomics Inc. Mitochondrial sites and genes associated with prostate cancer
USH2220H1 (en) * 2001-08-10 2008-07-01 Snp Consortium Identification and mapping of single nucleotide polymorphisms in the human genome
US20070203083A1 (en) * 2003-06-13 2007-08-30 Mootha Vamsi K Methods Of Regulating Metabolism And Mitochondrial Function
JP2005107404A (ja) 2003-10-01 2005-04-21 Matsushita Electric Ind Co Ltd 広角撮像光学系、並びにそれを備えた広角撮像装置、監視用撮像装置、車載用撮像装置及び投写装置

Also Published As

Publication number Publication date
EP2500436B1 (en) 2016-05-25
US9957555B2 (en) 2018-05-01
JP2014509862A (ja) 2014-04-24
JP6262533B2 (ja) 2018-01-17
US20140087378A1 (en) 2014-03-27
US20190330691A1 (en) 2019-10-31
EP2500436A1 (en) 2012-09-19
EP2686440A1 (en) 2014-01-22
EP2686440B1 (en) 2017-12-20
WO2012123588A1 (en) 2012-09-20
US11566280B2 (en) 2023-01-31

Similar Documents

Publication Publication Date Title
EP2500436B1 (en) Method, probe and kit for DNA in situ hybridation and use thereof
US20250263784A1 (en) Multiplex labeling of molecules by sequential hybridization barcoding
US20220026433A1 (en) Cleavable fluorescent tyramide for sensitive and multiplexed analysis of biological samples
US20210115504A1 (en) Multiplex labeling of molecules by sequential hybridization barcoding with rapid switching and rehybridization of probes
AU2014268710B2 (en) Transposition into native chromatin for personal epigenomics
Nuovo et al. A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with their putative targets
CN105612261B (zh) 作为生物标记物的miRNA-124
Dodel et al. TREX reveals proteins that bind to specific RNA regions in living cells
CA2840558C (en) Methods of detecting gene fusions using first and second nucleic acid probes
JP2011511949A5 (enExample)
JP6247934B2 (ja) 単一細胞において染色体構造および遺伝子発現を同時に検出するための方法
CN102732619B (zh) 一种miRNA的原位杂交探针及其检测试剂盒和应用
CN114945681A (zh) 用于连续核酸检测的方法
Ling et al. Technical innovations of signal amplification in fluorescence in situ hybridization
KR20220035958A (ko) 표적 분석에 관한 방법 및 조성물
Class et al. Patent application title: METHOD, PROBE AND KIT FOR DNA IN SITU HYBRIDIZATION AND USE THEREOF
CN113025690B (zh) 一种核酸探针组及其应用
US20160145686A1 (en) Biomarker for senescence and use thereof
Hansen et al. Transcription arrest induces formation of protective RNA granules in mitochondria
Hansen et al. Transcription arrest induces formation of RNA granules in mitochondria
KR102224910B1 (ko) Rna 은 제자리 혼성화 방법을 통한 aimp2-dx2 신호 자동화 검출 방법
Li et al. Integration of Demethylation-Switchable Deoxyribozyme with Controllable Self-Assembly of Branched DNA Nanostructures Enables In Situ Monitoring of FTO Demethylation in Living Cells
WO2022248889A1 (en) Assays
Smolina Application of PNA Openers for Fluorescence-Based Detection of Bacterial DNA
Bäumer Functional genetic analysis of motor neuron disease