DK2450438T3 - Cellulase-præparat indeholdende endoglucanaser fra to forskellige typer mikroorganismer - Google Patents

Cellulase-præparat indeholdende endoglucanaser fra to forskellige typer mikroorganismer Download PDF

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Publication number
DK2450438T3
DK2450438T3 DK10794231.0T DK10794231T DK2450438T3 DK 2450438 T3 DK2450438 T3 DK 2450438T3 DK 10794231 T DK10794231 T DK 10794231T DK 2450438 T3 DK2450438 T3 DK 2450438T3
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Denmark
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ser
gly
thr
ala
gin
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DK10794231.0T
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Kaoru Okakura
Koichiro Murashima
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Meiji Seika Pharma Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • DTEXTILES; PAPER
    • D06TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06MTREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Textile Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)

Claims (8)

1. Cellulase-præparat omfattende endoglucanaser, der er afledt af to forskellige typer mikroorganismer, hvor præparatet er en kombination af en hvilken som helst af følgende (a) og (b): (a) en kombination af en endoglucanase, der er klassificeret i GH-familie 5, og en endoglucanase, der er klassificeret i GH-familie 12, og (b) en kombination af en endoglucanase, der er klassificeret i GH-familie 12, og en endoglucanase, der er klassificeret i GH-familie 45, hvor endoglucanasen, der er klassificeret i GH-familie 5, er et protein med en af aminosyresekvensen ifølge SEQ ID NO: 2 og aminosyresekvensen, hvori 10 eller færre af aminosyrerne er deleteret, substitueret, indføjet eller tilsat, hvor endoglucanasen, der er klassificeret i GH-familie 12, er et protein med en af aminosyresekvensen ifølge SEQ ID NO: 4 og aminosyresekvensen, hvori 10 eller færre af aminosyrerne er deleteret, substitueret, indføjet eller tilsat, hvor endoglucanasen, der er klassificeret i GH-familie 45, er et protein med en af aminosyresekvensen ifølge SEQ ID NO: 6 og aminosyresekvensen, hvori 10 eller færre af aminosyrerne er deleteret, substitueret, indføjet eller tilsat.
2. Cellulase-præparat ifølge krav 1, hvor endoglucanaserne er rekombinante proteiner.
3. Cellulase-præparat ifølge krav 1 eller 2, hvor endoglucansaserne af kombination (a) og (b) hver især er indeholdt i en mængde på mindst 10 vægt-% af de samlede cellulaser.
4. Cellulase-præparat ifølge krav 3, hvor endoglucansaserne af kombination (a) og (b) hver især er indeholdt i en mængde på mindst 20 vægt-% af de samlede cellulaser.
5. Fremgangsmåde til fremstilling af cellulase-præparatet ifølge krav 1, hvilken fremgangsmåde omfatter trinnet med at dyrke en transformant, der er opnået ved at indføre DNAer, der koder for to typer af endoglucanaserne, i en enkelt værtscelle.
6. Fremgangsmåde til fremstilling af cellulase-præparatet ifølge krav 1, hvor værtscellen er en filamentøs svamp.
7. Fremgangsmåde til fremstilling af en forbedret cellulose-holdig fiber, hvilken fremgangsmåde omfatter trinnet med at bringe en cellulose-holdig fiber i kontakt med cellulase-præparatet ifølge et af kravene 1 til 4.
8. Fremgangsmåde til fremstilling af sukker fra biomasse, hvilken fremgangsmåde omfatter trinnet med at bringe en cellulose-holdig biomasse i kontakt med cellulase-præparatet ifølge et af kravene 1 til 4.
DK10794231.0T 2009-07-03 2010-07-01 Cellulase-præparat indeholdende endoglucanaser fra to forskellige typer mikroorganismer DK2450438T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2009159109 2009-07-03
PCT/JP2010/061263 WO2011002063A1 (ja) 2009-07-03 2010-07-01 2種類の異なる微生物に由来するエンドグルカナーゼを含んでなるセルラーゼ調製物

Publications (1)

Publication Number Publication Date
DK2450438T3 true DK2450438T3 (da) 2015-12-07

Family

ID=43411131

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DK10794231.0T DK2450438T3 (da) 2009-07-03 2010-07-01 Cellulase-præparat indeholdende endoglucanaser fra to forskellige typer mikroorganismer

Country Status (9)

Country Link
US (1) US9181537B2 (da)
EP (1) EP2450438B1 (da)
JP (1) JP5745404B2 (da)
CN (1) CN102471764B (da)
BR (1) BRPI1016260A2 (da)
CA (1) CA2766025A1 (da)
DK (1) DK2450438T3 (da)
ES (1) ES2553223T3 (da)
WO (1) WO2011002063A1 (da)

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4832864A (en) 1987-09-15 1989-05-23 Ecolab Inc. Compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
JPH09500667A (ja) * 1993-07-12 1997-01-21 ノボ ノルディスク アクティーゼルスカブ 2つのセルラーゼ成分を含んでなる洗剤組成物
JP3626203B2 (ja) 1995-11-15 2005-03-02 ノボザイムス アクティーゼルスカブ 染色されたデニムの複合糊抜及び「ストーンウォッシュ」方法
WO1998003667A1 (fr) 1996-07-24 1998-01-29 Meiji Seika Kaisha, Ltd. SYSTEMES DE PRODUCTION DE GRANDES QUANTITES DE PROTEINES OU DE PEPTIDES A L'AIDE DE MICRO-ORGANISMES DU GENRE $i(HUMICOLA)
WO1998011239A1 (fr) 1996-09-13 1998-03-19 Meiji Seika Kaisha, Ltd. Sequence de regulation des genes de la cellulase cbh1 provenant de trichoderma viride et systeme de production en serie de proteines ou de peptides utilisant une telle sequence
US5989899A (en) * 1996-12-23 1999-11-23 Genencor International, Inc. Oversized cellulase compositions for use in detergent compositions and in the treatment of textiles
AU7452898A (en) * 1997-05-27 1998-12-30 Meiji Seika Kaisha Ltd. Cellulase preparation containing highly active cellulase sce3
AU2001268603B2 (en) * 2000-06-26 2006-11-09 University Of Florida Research Foundation, Inc. Methods and compositions for simultaneous saccharification and fermentation
US7364890B2 (en) * 2001-07-28 2008-04-29 Midwest Research Institute Thermal tolerant avicelase from Acidothermus cellulolyticus
ES2575526T3 (es) * 2003-12-03 2016-06-29 Meiji Seika Pharma Co., Ltd. Endoglucanasa STCE y preparación de celulasa que contiene la misma
WO2005056787A1 (ja) 2003-12-08 2005-06-23 Meiji Seika Kaisha, Ltd. 界面活性剤に耐性なセルラーゼ及びその変換方法
CN103494661B (zh) 2006-07-20 2016-03-30 奥巴斯尼茨医学公司 可生物吸收的聚合物医疗器械
CA2680656C (en) 2007-03-12 2016-02-02 Meiji Seika Kaisha, Ltd. Endoglucanase ppce and cellulase preparation containing the same
DK2069491T3 (da) * 2007-05-10 2018-04-09 Novozymes Inc Sammensætninger og fremgangsmåder til forbedring af nedbrydningen eller omdannelsen af celluloseholdigt materiale
BRPI0812920A2 (pt) * 2007-06-08 2014-10-14 Danisco Us Inc Genencor Div Sistema de expressão de celulase heteróloga e homóloga
WO2009132008A2 (en) * 2008-04-21 2009-10-29 Chromatin, Inc. Methods and materials for processing a feedstock
JP2011182675A (ja) * 2010-03-05 2011-09-22 Toyota Central R&D Labs Inc セルラーゼ組成物及びその利用

Also Published As

Publication number Publication date
US20120100578A1 (en) 2012-04-26
WO2011002063A8 (ja) 2011-02-24
EP2450438B1 (en) 2015-09-30
CN102471764A (zh) 2012-05-23
WO2011002063A1 (ja) 2011-01-06
US9181537B2 (en) 2015-11-10
EP2450438A9 (en) 2012-10-24
JPWO2011002063A1 (ja) 2012-12-13
EP2450438A1 (en) 2012-05-09
CA2766025A1 (en) 2011-01-06
BRPI1016260A2 (pt) 2019-09-24
CN102471764B (zh) 2014-07-09
JP5745404B2 (ja) 2015-07-08
ES2553223T3 (es) 2015-12-07
EP2450438A4 (en) 2013-01-09

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