DK2404996T3 - Transkriptionsregulatoriske faktorer til mannaser eller cellulaser, og gener til transkriptionsregulatoriske faktorer - Google Patents

Transkriptionsregulatoriske faktorer til mannaser eller cellulaser, og gener til transkriptionsregulatoriske faktorer Download PDF

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Publication number
DK2404996T3
DK2404996T3 DK10748724.1T DK10748724T DK2404996T3 DK 2404996 T3 DK2404996 T3 DK 2404996T3 DK 10748724 T DK10748724 T DK 10748724T DK 2404996 T3 DK2404996 T3 DK 2404996T3
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Denmark
Prior art keywords
hydrolases
manr
seq
gene
dna
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DK10748724.1T
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English (en)
Inventor
Masahiro Ogawa
Yasuji Koyama
Masayuki Machida
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Noda Inst For Scientific Res
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Publication of DK2404996T3 publication Critical patent/DK2404996T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Claims (9)

1. Protein: (a) omfattende en aminosyresekvens repræsenteret ved SEQ ID NO: 2; eller (b) omfattende aminosyresekvensen repræsenteret ved SEQ ID NO: 2 hvor en til fem aminosyrer er fjernet eller SEQ ID NO: 2 hvor en til fem aminosyrer er tilføjet, og der har en positiv reguleringsevne på transkriptionen af gener for mannan-hydrolaser eller cellulosehydrolaser.
2. Gen der koder for et protein: (a) omfattende en aminosyresekvens repræsenteret ved SEQ ID NO: 2; eller (b) omfattende aminosyresekvensen repræsenteret ved SEQ ID NO: 2 hvor en til fem aminosyrer er fjernet eller SEQ ID NO: 2 hvor en til fem aminosyrer er tilføjet og der har en positiv reguleringsevne på transkriptionen af gener for mannan-hydrolaser eller cellulosehydrolaser.
3. Genet ifølge krav 2, hvilket gen består af et DNA omfattende basesekvensen repræsenteret ved SEQ ID NO: 1.
4. Genet ifølge krav 3, hvor genet er et cDNA.
5. Rekombinant vektor omfattende genet ifølge et hvilket som helst af kravene 2 til 4.
6. Ikke-human transformant omfattende den rekombinante vektor ifølge krav 5.
7. Fremgangsmåde til fremstillingen af transkriptionsregulatoriske faktorer, omfattende at dyrke af transformanten ifølge krav 6 og at genvinde de transkriptionsregulatoriske faktorer fra det resulterende dyrkningsprodukt.
8. Fremgangsmåde til fremstillingen af mannan-hydrolaser eller cellulose-hydrolaser, omfattende at dyrke en transformant omfattende en rekombinant vektor og at genvinde mannan-hydrolaserne eller cellulose-hydrolaserne fra det resulerende dyrkningsprodukt, hvor den rekombinante vektor omfatter: (i) et gen der koder for et protein: (a) omfattende en aminosyresekvens repræsenteret ved SEQ ID NO: 2; (b) omfattende aminosyresekvensen repræsenteret ved SEQ ID NO: 2 hvor en til fem aminosyrer er fjernet, substitueret eller tilføjet og der har en positiv reguleringsevne på transkriptionen af gener for mannan-hydrolaser eller cellulose-hydrolaser; eller (c) omfattende en aminosyresekvens der har 70% eller derover identitet med aminosyresekvensen repræsenteret ved SEQ ID NO: 2 eller en aminosyresekvens med 70% eller derover identitet med et delvist fragment af SEQ ID NO:2, hvor sekvensen har en positiv reguleringsevne på transkriptionen af gener for mannan-hydrolaser eller cellulosehydrolaser; eller (ii) et gen bestående af et DNA: (a) omfattende en basesekvens repræsenteret ved SEQ ID NO: 1; eller (b) der har 70% eller derover identitet med DNA af basesekvensen repræsenteret ved SEQ ID NO: 1, og der koder for et protein der har en positiv reguleringsevne på transkriptionen af gener for mannan-hydrolaser eller cellulosehydrolaser.
9. Fremgangsmåde til forøgelse af produktionen af mannan-hydrolaser eller cellulose-hydrolaser i en værtscelle fra en art hvis mannan-hydrolase eller cellulose-hydrolase er reguleret af protein der har aminosyresekvensen af SEQ ID NO:2, hvilken fremgangsmåde omfatter tvungen ekspression afen rekombinant vektor ifølge krav 8 i værtscellen.
DK10748724.1T 2009-03-04 2010-03-02 Transkriptionsregulatoriske faktorer til mannaser eller cellulaser, og gener til transkriptionsregulatoriske faktorer DK2404996T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2009051323 2009-03-04
PCT/JP2010/053283 WO2010101129A1 (ja) 2009-03-04 2010-03-02 マンナン加水分解酵素群又はセルロース加水分解酵素群転写制御因子及び該転写制御因子遺伝子

Publications (1)

Publication Number Publication Date
DK2404996T3 true DK2404996T3 (da) 2016-09-19

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DK10748724.1T DK2404996T3 (da) 2009-03-04 2010-03-02 Transkriptionsregulatoriske faktorer til mannaser eller cellulaser, og gener til transkriptionsregulatoriske faktorer

Country Status (5)

Country Link
US (1) US8877915B2 (da)
EP (1) EP2404996B1 (da)
JP (1) JP5704609B2 (da)
DK (1) DK2404996T3 (da)
WO (1) WO2010101129A1 (da)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2734632A1 (en) * 2011-07-21 2014-05-28 The Regents of the University of California Transcription factors for the production of cellulose degrading enzymes
JP6368968B2 (ja) * 2014-01-22 2018-08-08 本田技研工業株式会社 形質転換体の製造方法、糖化酵素の生産方法、及び草木類バイオマスの糖化処理方法
JP6261039B2 (ja) * 2014-03-04 2018-01-17 キッコーマン株式会社 転写制御因子ｍａｎＲ及びｘｌｎＲを二重強制発現させた麹菌を用いる多糖類の分解方法
JP6360787B2 (ja) * 2014-12-04 2018-07-18 本田技研工業株式会社 麹菌変異株及び形質転換体
GB2589246A (en) 2018-05-16 2021-05-26 Synthego Corp Methods and systems for guide RNA design and use
US11970733B2 (en) 2018-11-01 2024-04-30 Synthego Corporation Methods for analyzing nucleic acid sequences
JP7412753B2 (ja) * 2019-06-27 2024-01-15 国立研究開発法人農業・食品産業技術総合研究機構 α－リノレン酸産生能が向上した微生物またはその培養物
JP7491542B2 (ja) * 2019-09-09 2024-05-28 株式会社フジワラテクノアート 基質培養物の製造方法及び基質培養物
CN114774392A (zh) * 2022-04-12 2022-07-22 昆明学院 一种甘露聚糖酶及其应用

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US5989870A (en) * 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
DK48693D0 (da) 1993-04-30 1993-04-30 Novo Nordisk As Enzym
US6489279B2 (en) * 1998-05-05 2002-12-03 The Procter & Gamble Company Laundry and cleaning compositions containing xyloglucanase enzymes
CA2331199C (en) * 1998-06-10 2012-10-23 Markus Sakari Kauppinen Isolated mannanases for use in treating cellulosic or synthetic fibers
JP4439641B2 (ja) 1999-11-19 2010-03-24 キリンホールディングス株式会社 新規マンナナーゼ、その製造法および用途
JP2003164286A (ja) 2001-11-30 2003-06-10 Noda Inst For Scient Res マンナナーゼおよびマンナナーゼ遺伝子
US7186540B2 (en) 2001-12-27 2007-03-06 National Institute of Advanced Indusrtial Science and Technology Thermostable glutaminase and thermostable glutaminase gene
JP2005176602A (ja) * 2001-12-27 2005-07-07 National Institute Of Advanced Industrial & Technology 麹菌遺伝子
NZ578309A (en) * 2007-01-30 2012-06-29 Verenium Corp Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
CN101952437B (zh) * 2007-10-03 2014-04-09 维莱尼姆公司 木聚糖酶、编码它们的核酸以及其制备和应用方法

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Publication number Publication date
US8877915B2 (en) 2014-11-04
EP2404996A1 (en) 2012-01-11
US20120142051A1 (en) 2012-06-07
EP2404996B1 (en) 2016-08-24
EP2404996A4 (en) 2013-02-27
JP5704609B2 (ja) 2015-04-22
WO2010101129A1 (ja) 2010-09-10
JPWO2010101129A1 (ja) 2012-09-10

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