DK2393919T3 - Ny permanent human cellelinje - Google Patents
Ny permanent human cellelinje Download PDFInfo
- Publication number
- DK2393919T3 DK2393919T3 DK10721938.8T DK10721938T DK2393919T3 DK 2393919 T3 DK2393919 T3 DK 2393919T3 DK 10721938 T DK10721938 T DK 10721938T DK 2393919 T3 DK2393919 T3 DK 2393919T3
- Authority
- DK
- Denmark
- Prior art keywords
- nucleic acid
- cells
- expression
- cell
- antigen
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/04—Immortalised cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/24—Vectors characterised by the absence of particular element, e.g. selectable marker, viral origin of replication
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Pregnancy & Childbirth (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Claims (5)
1. Fremgangsmåde til fremstilling af en permanent human amniocytcellelinje omfattende: a) transfektion af primære humane amniocytceller med et nukleinsyremolekyle, der omfatter en nukleinsyresekvens, som koder for de adenovirale genprodukter E1A og E1B, og b) efterfølgende transfektion af den i trin a) opnåede permanente humane amniocytcellelinje med et nukleinsyremolekyle, der omfatter en nukleinsyresekvens, som koder for det store T-antigen fra SV40 eller det nukleare antigen-1 (EBNA-1)fra Epstein-Barr-virus (EBV).
2. Fremgangsmåde ifølge krav 1, hvor den nukleinsyresekvens, som koder for de adenovirale genfunktioner E1A og E1B, stammer fra et humant adenovirus, især fra humant adenovirus serotype 5.
3. Fremgangsmåde ifølge krav 2, hvor den nukleinsyresekvens, som koder for de adenovirale genprodukter E1A og E1B, omfatter nukleotiderne 1 til 4344, 505 til 3522 eller nukleotiderne 505 til 4079 af det humane adenovirus serotype 5.
4. Fremgangsmåde ifølge krav 1, hvor den nukleinsyresekvens, som koder for det store T-antigen fra SV40, desuden omfatter nukleinsyresekvensen for en promotor, som er valgt fra gruppen CMV-promotor, CAG-promotor og RSV-promotor, nukleinsyresekvensen for SV40 SD/SA (intran) og nukleinsyresekvensen for SV40 polyA, og nukleinsyresekvensen for det nukleare antigen-1 (EBNA-1) fra Epstein-Barr-virus (EBV) desuden omfatter nukleinsyresekvensen for en promotor, som er valgt fra gruppen CMV-promotor, CAG-promotor og RSV-promotor, nukleinsyresekvensen for SV40 SD/SA (intran) og nukleinsyresekvensen for SV40 polyA.
5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor der i stedet for trin a) anvendes humane amniocytceller, som allerede er immortaliseret med et nukleinsyremolekyle, der omfatter en nukleinsyresekvens, som koder for de adenovirale genprodukter E1A og E1B.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102009003439A DE102009003439A1 (de) | 2009-02-05 | 2009-02-05 | Neue permanente humane Zelllinie |
| PCT/DE2010/075012 WO2010094280A1 (de) | 2009-02-05 | 2010-02-05 | Neue permanente humane zelllinie |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2393919T3 true DK2393919T3 (da) | 2015-09-21 |
Family
ID=42316055
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10721938.8T DK2393919T3 (da) | 2009-02-05 | 2010-02-05 | Ny permanent human cellelinje |
| DK15000822.5T DK2913395T3 (da) | 2009-02-05 | 2010-02-05 | Hidtil ukendt permanent human cellelinje |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15000822.5T DK2913395T3 (da) | 2009-02-05 | 2010-02-05 | Hidtil ukendt permanent human cellelinje |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US9371512B2 (da) |
| EP (2) | EP2913395B1 (da) |
| JP (1) | JP2012516690A (da) |
| KR (1) | KR101695349B1 (da) |
| CN (1) | CN102369279A (da) |
| AU (1) | AU2010214951B2 (da) |
| BR (1) | BRPI1008083B8 (da) |
| CA (1) | CA2751497C (da) |
| DE (2) | DE102009003439A1 (da) |
| DK (2) | DK2393919T3 (da) |
| ES (2) | ES2547700T3 (da) |
| HU (1) | HUE036707T2 (da) |
| IL (1) | IL214421A (da) |
| LT (1) | LT2913395T (da) |
| PL (2) | PL2393919T3 (da) |
| PT (2) | PT2393919E (da) |
| RU (1) | RU2569181C2 (da) |
| SG (1) | SG173484A1 (da) |
| TW (1) | TW201033363A (da) |
| WO (1) | WO2010094280A1 (da) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2784155B1 (en) * | 2011-11-24 | 2018-06-13 | Viromed Co. Ltd. | Adenovirus producing novel cell line and the use thereof |
| WO2014115087A2 (en) * | 2013-01-22 | 2014-07-31 | Cencor Biotech Ltd. | A method for the preparation of recombinant human prothrombin and fibrinogen |
| EP3205719A1 (en) * | 2016-02-15 | 2017-08-16 | CEVEC Pharmaceuticals GmbH | Cell lines for producing recombinant glycoproteins with di-antennary n-glycans, methods using the same, and recombinant glycoproteins |
| EP3382014A1 (en) | 2017-03-29 | 2018-10-03 | CEVEC Pharmaceuticals GmbH | Recombinant glycoproteins with reduced antennary fucosylation |
| EP3736286A1 (en) | 2019-05-09 | 2020-11-11 | Biotest AG | Single chain factor viii molecule |
| AU2020342349A1 (en) | 2019-09-02 | 2022-03-03 | Biotest Ag | Factor VIII protein with increased half-life |
| EP3785726A1 (en) | 2019-09-02 | 2021-03-03 | Biotest AG | Factor viii protein with increased half-life |
| CN114929261A (zh) | 2020-02-17 | 2022-08-19 | 生物测试股份公司 | 凝血因子viii的皮下施用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5733782A (en) * | 1993-10-25 | 1998-03-31 | Creative Biomolecules, Inc. | Methods and compositions for high protein production from non-native DNA |
| US7410798B2 (en) * | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
| US20050170463A1 (en) * | 1999-04-15 | 2005-08-04 | Abraham Bout | Recombinant protein production in permanent amniocytic cells that comprise nucleic acid encoding adenovirus E1A and E1B proteins |
| DE19955558C2 (de) | 1999-11-18 | 2003-03-20 | Stefan Kochanek | Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren |
| US6558948B1 (en) * | 1999-11-23 | 2003-05-06 | Stefan Kochanek | Permanent amniocytic cell line, its production and use for the production of gene transfer vectors |
| RU2267538C2 (ru) * | 2000-09-08 | 2006-01-10 | Айова Стейт Юниверсити Рисерч Фаундейшн, Инк. | Способ скрининга животных с повышенным уровнем численности приплода и/или предпочтительными признаками качества мяса, белок prkag3 (варианты) и кодирующая его нуклеотидная последовательность (варианты) |
| DE102005054628A1 (de) * | 2005-11-16 | 2007-05-24 | Cevec Pharmaceuticals Gmbh | Verfahren zur Herstellung von permanenten humanen Zelllinien |
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2009
- 2009-02-05 DE DE102009003439A patent/DE102009003439A1/de not_active Ceased
-
2010
- 2010-02-05 PL PL10721938T patent/PL2393919T3/pl unknown
- 2010-02-05 EP EP15000822.5A patent/EP2913395B1/de active Active
- 2010-02-05 HU HUE15000822A patent/HUE036707T2/hu unknown
- 2010-02-05 CN CN201080014527XA patent/CN102369279A/zh active Pending
- 2010-02-05 EP EP10721938.8A patent/EP2393919B1/de active Active
- 2010-02-05 JP JP2011548530A patent/JP2012516690A/ja active Pending
- 2010-02-05 ES ES10721938.8T patent/ES2547700T3/es active Active
- 2010-02-05 RU RU2011136451/10A patent/RU2569181C2/ru active IP Right Revival
- 2010-02-05 DE DE112010000285T patent/DE112010000285A5/de not_active Withdrawn
- 2010-02-05 CA CA2751497A patent/CA2751497C/en active Active
- 2010-02-05 PT PT107219388T patent/PT2393919E/pt unknown
- 2010-02-05 PL PL15000822T patent/PL2913395T4/pl unknown
- 2010-02-05 AU AU2010214951A patent/AU2010214951B2/en active Active
- 2010-02-05 SG SG2011055282A patent/SG173484A1/en unknown
- 2010-02-05 LT LTEP15000822.5T patent/LT2913395T/lt unknown
- 2010-02-05 TW TW099103626A patent/TW201033363A/zh unknown
- 2010-02-05 ES ES15000822.5T patent/ES2642837T3/es active Active
- 2010-02-05 WO PCT/DE2010/075012 patent/WO2010094280A1/de active Application Filing
- 2010-02-05 KR KR1020117020450A patent/KR101695349B1/ko active IP Right Grant
- 2010-02-05 DK DK10721938.8T patent/DK2393919T3/da active
- 2010-02-05 BR BRPI1008083A patent/BRPI1008083B8/pt active IP Right Grant
- 2010-02-05 US US13/147,775 patent/US9371512B2/en active Active
- 2010-02-05 PT PT150008225T patent/PT2913395T/pt unknown
- 2010-02-05 DK DK15000822.5T patent/DK2913395T3/da active
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2011
- 2011-08-02 IL IL214421A patent/IL214421A/en active IP Right Grant
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