DK2337837T4 - Alpha-amylase blandinger og fremgangsmåder til anvendelse deraf - Google Patents

Alpha-amylase blandinger og fremgangsmåder til anvendelse deraf Download PDF

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DK2337837T4
DK2337837T4 DK09792446.8T DK09792446T DK2337837T4 DK 2337837 T4 DK2337837 T4 DK 2337837T4 DK 09792446 T DK09792446 T DK 09792446T DK 2337837 T4 DK2337837 T4 DK 2337837T4
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amylase
alpha
starch
amino acid
amys
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DK2337837T3 (da
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Bradley A Paulson
Vivek Sharma
Jayarama Shetty
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Danisco Us Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681—Chemically modified or immobilised enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)

Claims (17)

  1. ALPHA-AMYLASE BLANDINGER OG FREMGANGSMÅDER TIL ANVENDELSE DERAF PATENTKRAV
    1. Fremgangsmåde til flydendegørelse af stivelse, hvilken fremgangsmåde omfatter: tilføjelse af en amylase-blanding til en opløsning omfattende stivelse og flydendegørelse af opløsningen, der omfatter stivelse, for at danne en opslæmning, hvor amylase-blandingen omfatter: (i) en B. stearothermophilus alpha-amylase (AmyS), der omfatter en amino syre sekvens med mindst 85 % sekvensidentitet med AmyS ifølge SEQ ID NO: 1, hvor AmyS har alpha-amylase-aktivitet, og hvor aminosyren ved position S242 er substitueret, ved anvendelse af aminosyretalsystemet ifølge SEQ ID NO: 2, hvor substitutionen er S242Q eller S242A; og (ii) en IS. licheniformis alpha-amylase, der omfatter en aminosyresekvens: ANLNGTLMQY FEWYTPNDGQ HWKRLQN DS A YLAEHGITAV WIPPAYKGTS QADVGYGAYD LYDLGEFHQK GTVRTKYGTK GELQSAIKSL HSRDINVYGD VV1NHKGGAD ATEDVTAVEV DPADRNRVIS GEYLIKAWI H FHFPGRGSTY SDFKWHWYHF DGTDWDESRK LNRIYKFQGK AWDWEVSSEN GNYDYLMYAD IDYDHPDVVA E1KRWGTWYA NELQLDGFRL DAVKHIKFSF LRDWVNHVRE KTGKEMFTVA EYWQNDLGAL ENYLNKTNFN HSVFDVPLHY QFHAASTQGG GYDMRKLLNG TVVSKHPLKS VTFVDNHDTQ PGQSLESTVQ TWFKPLAYAF ILTRESGYPQ VFYGDMYGTK GDSQREIPAL KHKIEPILKA RKQYAYGAQH DYFDHHDIVG WTREGDSSVA NSGLAALITD GPGGAKRMYV GRQNAGETWH DITGNRSEPV VINSEGWGEF HVNGGSVSIY VQR.
  2. 2. Fremgangsmåde ifølge krav 1, hvor stivelsen opnås fra plantemateriale, majs, milokom, mg, byg, hvede, durra, havre, ris, klid, kassava, hirse, kartoffel, sød kartoffel eller tapioka, og eventuelt hvor stivelsen er granulær stivelse fra enten en tør eller våd formalingsmetode.
  3. 3. Fremgangsmåde ifølge krav 1 eller krav 2, hvilken fremgangsmåde endvidere omfatter: (i) et primært og/'eller sekundært flydendegørelsestrin, herunder tilføjelse af yderligere substrat til opslæmningen i det primære og/'eller sekundære flydendegørelsestrin; og/eller (ii) tilføjelse af et fytinsyrehydrolyserende enzym; og/eller (iii) saccharifrcering af den flydende stivelse for at opnå gæringsdygtigt sukker, og eventuelt gæring af det gæringsdygtige sukker under egnede gæringsforhold for at opnå alkoholiske slutprodukter.
  4. 4. Fremgangsmåde ifølge krav 3 (iii), hvor de alkoholiske slutprodukter er ethanol eller butanol.
  5. 5. Fremgangsmåde ifølge krav 1, hvor: (i) flydendegørelsens pH ligger i intervallet fra pH 4,5 til 5,4, og syreneutraliserende kemikalier ikke tilføjes i trinnet med flydendegørelsesprocessen; eller (ii) flydendegørelsens pH ligger i intervallet fra pH 4,8 til 5.8, og syreneutraliserende kemikalier ikke tilføjes i trinnet med flydendegørelsesprocessen.
  6. 6. Fremgangsmåde til opnåelse af et gæringsdygtigt substrat, hvilken fremgangsmåde omfatter: etablering af kontakt mellem en opslæmning af malet kom, der indeholder granulær stivelse, med både et fytinsyrehydrolyserende enzym og en blanding af alpha-amylaser ved en temperatur på 0-30 °C lavere end stivelsens gelatineringstemperatur, hævelse af temperaturen til over gelatineringstemperaturen, hydrolysering af den gelatinerede stivelse og opnåelse af et gæringsdygtigt substrat, hvor blandingen af alpha-amylaser omfatter: (i) en B. stearothermophilus alpha-amylase (AmyS), der omfatter en aminosyresekvens med mindst 85 % sekvensidentitet med AmyS ifølge SEQ ID NO: 1, hvor AmyS har alpha-amylase-aktivitet, og hvor aminosyren ved position S242 er substitueret, ved anvendelse af aminosyretalsystemet ifølge SEQ ID NO: 2, hvor substitutionen er S242Q eller S242A; og (ii) en B. licheniformis alpha-amylase, der omfatter en aminosyresekvens: ANLNGTLMQY FEWYTPNDGQ HWKRLQNDSA YLAEHGITAV WIPPAYKGTS QADVGYGAYD LYDLGEFHQK GTVRTKYGTK GELQSAIKSL HSRDINVYGD VVINHKGGAD ATEDVTAVEV DPADRNRVIS GEYLIKAWTH FHFPGRGSTY SDFKWHWYHF DGTDWDESRK LNRIYKFQGK AWDWEVSSEN GNYDYLMYAD 1DYDHPDVVA EIKRWGTWYA NELQLDGFRL DAVKHIKFSF LRDWVNHVRE KTGKEMFTVA EYWQNDLGAL ENYLNKTNFN HSVFDVPLHY QFHAASTQGG GYDMRKLLNG TWSKHPLKS VTFVDNHDTQ PGQSLESTVQ TWFKPLAYAF ILTRESGYPQ VFYGDMYGTK. GDSQREIPAL K.HKIEPILKA RKQYAYGAQH DYFDHHDIVG WTREGDSSVA NSGLAAL1TD GPGGAKRMYV GRQNAGETWH DITGNRSEPV VINSEGWGEF HVNGGSVSIY VQR.
  7. 7. Fremgangsmåde til fremstilling af et gæringsdygtigt sukker, hvilken fremgangsmåde omfatter: (a) blanding af malet stivelsesholdigt materiale med vand og bærme, hvor bærmen ligger i intervallet fra 10 til 70 % volumen/volumen, og opnåelse af en opslæmning, der omfatter stivelse og har et tørstofindhold (ds) på 20 til 50 % vægt/vægt, (b) behandling af opslæmningen med en fytase før eller samtidig med flydendegørelse af stivelsen, (c) flydendegørelse af stivelsen, (d) tilføjelse en alpha-amylase-blanding til stivelsen enten under trin (b) og/eller samtidig med trinnet med flydendegørelse, og (e) saccharificering af den flydende stivelse for at opnå gæringsdygtige sukkere, hvor pH’et ikke er justeret under nogen som helst af trinnene (a), (b), (c), (d) eller (e), hvor alpha-amylase-blandingen omfatter: (i) en B. stearothermophilus alpha-amylase (AmyS), der omfatter en aminosyresekvens med mindst 85 % sekvensidentitet med AmyS ifølge SEQ ID NO: 1, hvor AmyS har alpha-amylase-aktivitet, og hvor aminosyren ved position S242 er substitueret, ved anvendelse af aminosyretalsystemet ifølge SEQ ID NO: 2, hvor substitutionen er S242Q eller S242A; og (ii) en B. licheniformis alpha-amylase, der omfatter en aminosyresekvens: ANLNGTLMQY FEWYTPNDGQ HWKRLQNDSA YLAEHGITAV WIPPAYKGTS QADVGYGAYD LYDLGEFHQK GTVRTKYGTK GELQSAIKSL HSRDINVYGD VVINHKGGAD ATEDVTAVEV DPADRNRVIS GEYL1XAWTH FHFPGRGSTY SDFKWHWYHF DGTDWDESRK LNR1YKFQGK AWDWEVSSEN GNYDYLMYAD IDYDFIPDWA E1KRWGTWYA NELQLDGFRL DAVKHIKFSF LRDWVNHVRE KTGKEMFTVA EYWQNDLGAL ENYLNKTNFN HSVFDVPLHY QFHAASTQGG GYDMRKLLNG TVVSKIIPLKS VTFVDNHDTQ PGQSLESTVQ TWFKPLAYAF ILTRESGYPQ VFYGDMYGTK GDSQREIPAL KHKIEPILKA RKQYAYGAQH DYFDHHDIVG WTREGDSSVA NSGLAALITD GPGGAKRMYV GRQNAGETWH D1TGNRSEPV VINSEGWGEF HVNGGSVSTY VQR.
  8. 8. Fremgangsmåde ifølge krav 7, hvor: (i) det gæringsdygtige sukker er fjernet og oprenset eller isomeriseret; og/eller (ii) fytasen tilsættes før trinnet med flydendegørelse; og/eller (iii) alpha-amylase-blandingen tilsættes sammen med fytasen; og/eller (iv) en anden dosis af alpha-amylase-blandingen tilsættes under trinnet med flydendegørelse eller tilsættes under trinnet med saccharificering.
  9. 9. Fremgangsmåde til fremstilling af glucose, hvilken fremgangsmåde omfatter; etablering af kontakt mellem et stivelsessubstrat med en amylase-blanding, flydendegørelse af stivelsessubstratet, og inkubation af substratet ved høj temperatur for at fremstille et produkt, der omfatter glucose, hvor amylase-blandingen omfatter: (i) en B. stearothermophilus alpha-amylase (AmyS), der omfatter en aminosyresekvens med mindst 85 % sekvensidentitet med AmyS ifølge SEQ ID NO: 1, hvor AmyS har alpha-amylase-aktivitet, og hvor aminosyren ved position S242 er substitueret, ved anvendelse af aminosyretalsystemet ifølge SEQ ID NO: 2, hvor substitutionen er S242Q eller S242A; og (ii) en B. licheniformis alpha-amylase, der omfatter en aminosyresekvens: ANLNGTLMQY FEWYTPNDGQ HWKRLQNDSA YLAEHGITAV WIPPAYKGTS QADVGYGAYD LYDLGEFHQK GTVRTKYGTK GELQSAIKSL HSRDINVYGD VVINHKGGAD ATEDVTAVEV DPADRNRVIS GEYLIKAWTH FHFPGRGSTY SDFKWHWYHF DGTDWDESRK LNR1YKFQGK AWDWEVSSEN GNYDYLMYAD 1DYDHPDVVA ElKRWGTWYA NELQLDGFRL DAVKHIKFSF LRDWVNHVRE KTGKEMFTVA EYWQNDLGAL ENYLNKTNFN HSVFDVPLHY QFHAASTQGG GYDMRKLLNG TVVSKHPLKS VTFVDNHDTQ PGQSLESTVQ TWFKPLAYAF ILTRESGYPQ VFYGDMYGTK GDSQREIPAL KHKIEPILKA RKQYAYGAQH DYFDHHDIVG WTREGDSSVA NSGLAALITD GPGGAKRMYV GRQNAGETWH DITGNRSEPV VINSEGWGEF HVNGGSVSIY VQR.
  10. 10. Fremgangsmåde ifølge krav 9, hvor: (i) inkubationstrinnet er et sekundært trin med flydendegørelse; og/eller (ii) inkubationstrinnet udføres ved en temperatur på ca. 90-100 °C; og/eller (iii) inkubationen udføres i tilstrækkelig tid, til at produktet omfatter ca. 2-14 DE glucose, og eventuelt hvor inkubationen udføres i tilstrækkelig tid, til at produktet omfatter ca. 10 DE glucose.
  11. 11. Fremgangsmåde ifølge krav 9, hvilken fremgangsmåde endvidere omfatter saccharificcring af produktet for at fremstille en glucose-rig opløsning.
  12. 12. Fremgangsmåde ifølge krav 11, hvor den glueose-rige opløsning omfatter 80-99 % glucose, fortrinsvis hvor den glueose-rige opløsning omfatter ca. 93-96 % glucose.
  13. 13. Fremgangsmåde ifølge krav 11, hvor saccharificeringen omfatter etablering af kontakt mellem glucoseproduktet og glueoamylase eller en enzymblanding, der omfatter en glueoamylase og en pullulanase.
  14. 14. Alpha-amylase-blanding, der omfatter: (i) en B. stearothermophilus alpha-amylase (AmyS), der omfatter en aminosyresekvens med mindst 85 % sekvensidentitet med AmyS ifølge SEQ ID NO: 1, hvor AmyS har alpha-amylase-aktivitet, og hvor aminosyren ved position S242 er substitueret, ved anvendelse af aminosyretalsystemet ifølge SEQ ID NO: 2, hvor substitutionen er S242Q eller S242A; og (ii) en B licheniformis alpha-amylase, der omfatter en aminosyresekvens: ANLNGTLMQY FEWYTPNDGQ HWKRLQNDSA YLAEHG1TAV WfPPAYKGTS QADVGYGAYD LYDLGEFHQK GTVRTKYGTK GELQSAIKSL HSRDINVYGD WINHKGGAD ATEDVTAVEV DPADRNRVIS GEYLIKAWTH FHFPGRGSTY SDFKWFIWYHF DGTDWDESRK LNR1YKFQGK AWDWEVSSEN GNYDYLMYAD IDYDHPDVVA EIKRWGTWYA NELQLDGFRL DAVKH1KFSF LRDWVNHVRE KTGKEM.FTVA EYWQNDLGAL ENYLNKTNFN HSVFDVPLHY QFHAASTQGG GYDMRKLLNG TVVSKHPLKS VTFVDNHDTQ PGQSLESTVQ TWFKPLAYAF ILTRESGYPQ VFYGDMYGTK GDSQREIPAL KHKIEP1LKA RKQYAYGAQH DYFDHHD1VG WTREGDSSVA NSGLAALITD GPGGAKRMYV GRQNAGETWH DITGNRSEPV V1NSEGWGEF HVNGGSVS1Y VQR.
  15. 15. Alpha-amylaseblanding ifølge krav 14, hvilken blanding endvidere omfatter en fytase.
  16. 16. Alpha-amylaseblanding ifølge krav 14 eller krav 15, hvor blandingen omfatter et vægtforhold på ca. 40 % af AmyS med S242-substitutionen og ca. 60 % B. licheniformis alpha-amylase.
  17. 17. Alpha-amylaseblanding ifølge krav 14 eller krav 15, hvor vægtforholdet mellem AmyS og S242-substitutionen og /1. licheniformis alpha-amylase er 10:90.
DK09792446.8T 2008-09-25 2009-09-11 Alpha-amylase blandinger og fremgangsmåder til anvendelse deraf DK2337837T4 (da)

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PCT/US2009/056613 WO2010036515A1 (en) 2008-09-25 2009-09-11 Alpha-amylase blends and methods for using said blends

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Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI1010238A2 (pt) 2009-04-01 2015-08-25 Danisco Us Inc Composições e métodos que comprendem variantes de alfa-amilase com propriedades alteradas
ES2565060T3 (es) 2010-04-14 2016-03-31 Novozymes A/S Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos
CO6480105A1 (es) * 2011-01-04 2012-07-16 Promotora De Innovacion En Biotecnologia S A S Promitec Santander S A S Proceso con adicion dosificada de materia prima y enzima para la produccion de malto dextrinas y jarabes de glucosa altamente concentrados a partir de almidones.
EP2761013A1 (en) * 2011-09-29 2014-08-06 Danisco US Inc. Liquefaction and saccharification of granular starch at high concentration
US20160017305A1 (en) 2013-03-11 2016-01-21 Danisco Us Inc. Alpha-amylase combinatorial variants
WO2019113413A1 (en) 2017-12-08 2019-06-13 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
DK3720956T3 (da) 2017-12-08 2023-09-04 Novozymes As Alfa-amylasevarianter og polynukleotider, der koder for dem
WO2020086430A2 (en) * 2018-10-22 2020-04-30 Dupont Nutrition Biosciences Aps Enzymes for infusion mashing in adjunct brewing technical field
CA3159662A1 (en) 2019-11-22 2021-05-27 Novozymes A/S Method for obtaining an oat-based product
CA3202322A1 (en) 2020-12-14 2022-06-23 Anthony Newton Method of starch processing
WO2023170628A1 (en) * 2022-03-09 2023-09-14 Lallemand Hungary Liquidity Management Llc Bacterial and archaeal alpha-amylases
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2024137246A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
WO2024137250A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
WO2024137248A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization
WO2024137704A2 (en) 2022-12-19 2024-06-27 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast

Family Cites Families (144)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1296839A (da) 1969-05-29 1972-11-22
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
US3912590A (en) 1973-01-03 1975-10-14 Novo Industri As Procedure for liquefying starch
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
US4316956A (en) 1980-02-06 1982-02-23 Novo Industri A/S Fermentation process
US4335208A (en) 1980-03-11 1982-06-15 Novo Industri A/S Saccharification of starch hydrolysates
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
FR2498783B1 (fr) 1981-01-23 1988-03-04 Decis Mario Dispositif de controle automatique de presence
JPS57174089A (en) 1981-04-20 1982-10-26 Novo Industri As Chain dividing enzyme product
FR2543181B1 (fr) 1983-03-22 1985-07-26 Ugine Kuhlmann Procede ameliore de desencollage-blanchiment simultane des tissus
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
US4689297A (en) 1985-03-05 1987-08-25 Miles Laboratories, Inc. Dust free particulate enzyme formulation
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0218272B1 (en) 1985-08-09 1992-03-18 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5240632A (en) 1986-03-26 1993-08-31 Amway Corporation Machine dishwasher water spot control composition
DK311186D0 (da) 1986-06-30 1986-06-30 Novo Industri As Enzymer
US4933279A (en) 1986-07-09 1990-06-12 Novo Industri A/S Starch liquefaction with alpha amylase mixtures
CA1283376C (en) * 1986-07-09 1991-04-23 John O. Carroll .alpha.-AMYLASE MIXTURES FOR STARCH LIQUEFACTION
US4753748A (en) 1986-08-28 1988-06-28 Colgate-Palmolive Company Nonaqueous liquid automatic dishwashing detergent composition with improved rinse properties and method of use
DE3750450T2 (de) 1986-08-29 1995-01-05 Novo Industri As Enzymhaltiger Reinigungsmittelzusatz.
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
GB8629538D0 (en) 1986-12-10 1987-01-21 Unilever Plc Enzymatic dishwashing & rinsing composition
GB8629537D0 (en) 1986-12-10 1987-01-21 Unilever Plc Enzymatic dishwashing composition
GB2200132B (en) 1986-12-15 1991-09-18 Colgate Palmolive Co Stabilised enzyme systems and compositions containing them
EP0305216B1 (en) 1987-08-28 1995-08-02 Novo Nordisk A/S Recombinant Humicola lipase and process for the production of recombinant humicola lipases
GB8727135D0 (en) 1987-11-19 1987-12-23 Unilever Plc Machine dishwashing composition
GB8727659D0 (en) 1987-11-26 1987-12-31 Unilever Plc Machine dishwashing compositions
US5141664A (en) 1987-12-30 1992-08-25 Lever Brothers Company, A Division Of Conopco, Inc. Clear detergent gel compositions having opaque particles dispersed therein
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
DE68924654T2 (de) 1988-01-07 1996-04-04 Novonordisk As Spezifische Protease.
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
US5648263A (en) 1988-03-24 1997-07-15 Novo Nordisk A/S Methods for reducing the harshness of a cotton-containing fabric
JP2728531B2 (ja) 1988-03-24 1998-03-18 ノボ ノルディスク アクティーゼルスカブ セルラーゼ調製品
GB8813688D0 (en) 1988-06-09 1988-07-13 Unilever Plc Enzymatic dishwashing composition
GB8813687D0 (en) 1988-06-09 1988-07-13 Unilever Plc Enzymatic dishwashing & rinsing composition
DE3833047C2 (de) 1988-09-29 1993-12-16 Henkel Kgaa Saure, maschinell anwendbare Geschirreinigungsmittel
US4973419A (en) 1988-12-30 1990-11-27 Lever Brothers Company, Division Of Conopco, Inc. Hydrated alkali metal phosphate and silicated salt compositions
GB8905439D0 (en) 1989-03-09 1989-04-19 Unilever Plc Enzymatic dishwashing compositions
BR9006818A (pt) 1989-06-29 1991-08-06 Gist Brocades Nv Amilases microbianas mutantes com maior estabilidade termica,acida e/ou alcalina
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
GB2234980A (en) 1989-07-25 1991-02-20 Unilever Plc Detergent composition for machine dishwashers
CA2023529A1 (en) 1989-08-22 1991-02-23 Guido Waschenbach Dish-washer detergent composition
EP0429124A1 (en) 1989-11-21 1991-05-29 The Procter & Gamble Company Chlorine-free liquid automatic dishwashing compositions
JP3112937B2 (ja) 1990-04-14 2000-11-27 カリ―ヒエミー アクチエンゲゼルシヤフト アルカリ性バチルスーリパーゼ、これをコード化するｄｎａ配列およびこのリパーゼを生産するバチルス
ES2068586T5 (es) 1990-05-09 2004-12-01 Novozymes A/S Una preparacion de celulasa que comprende una enzima endoglucanasa.
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
US5814501A (en) 1990-06-04 1998-09-29 Genencor International, Inc. Process for making dust-free enzyme-containing particles from an enzyme-containing fermentation broth
ATE169671T1 (de) 1990-09-13 1998-08-15 Novo Nordisk As Lipase-varianten
WO1992006221A1 (en) 1990-10-05 1992-04-16 Genencor International, Inc. Methods for treating cotton-containing fabrics with cellulase
US5133892A (en) 1990-10-17 1992-07-28 Lever Brothers Company, Division Of Conopco, Inc. Machine dishwashing detergent tablets
EP0495258A1 (en) 1991-01-16 1992-07-22 The Procter & Gamble Company Detergent compositions with high activity cellulase and softening clays
ATE136055T1 (de) 1991-04-30 1996-04-15 Procter & Gamble Gerüstsubstanzhaltige flüssigwaschmittel mit borsäure-polyolkomplex zur ptoteolytischen enzyminhibierung
EP0511456A1 (en) 1991-04-30 1992-11-04 The Procter & Gamble Company Liquid detergents with aromatic borate ester to inhibit proteolytic enzyme
WO1992019729A1 (en) 1991-05-01 1992-11-12 Novo Nordisk A/S Stabilized enzymes and detergent compositions
US5231017A (en) 1991-05-17 1993-07-27 Solvay Enzymes, Inc. Process for producing ethanol
US5240633A (en) 1991-05-31 1993-08-31 Colgate-Palmolive Company Liquid automatic dishwashing composition containing enzymes
AU654184B2 (en) 1991-05-31 1994-10-27 Colgate-Palmolive Company, The Improved phosphate-containing powder automatic dishwashing composition with enzymes
AU656375B2 (en) 1991-05-31 1995-02-02 Colgate-Palmolive Company, The Powdered automatic dishwashing composition containing enzymes
AU654009B2 (en) 1991-05-31 1994-10-20 Colgate-Palmolive Company, The Phosphate-containing powder automatic dishwashing composition with enzymes
AU655274B2 (en) 1991-05-31 1994-12-15 Colgate-Palmolive Company, The Nonaqueous liquid, phosphate-free, improved automatic dishwashing composition containing enzymes
DK0518720T3 (da) 1991-05-31 1996-01-29 Colgate Palmolive Co Ikke-vandigt flydende maskinopvaskemiddel indeholdende enzymer
DE69133633D1 (de) 1991-06-11 2010-07-08 Genencor Int Cellulasezusammensetzungen mit einem Defizit an Komponenten des Typs-CBH I enthaltende Reinigungsmittelzusammensetzungen
GB9118242D0 (en) 1991-08-23 1991-10-09 Unilever Plc Machine dishwashing composition
ES2100985T3 (es) 1991-08-29 1997-07-01 Benckiser Gmbh Joh A Agente neutro para el lavado mecanico de vajillas.
GB9119936D0 (en) 1991-09-17 1991-10-30 Unilever Plc Aqueous liquid cleaning compositions
US5879920A (en) 1991-10-07 1999-03-09 Genencor International, Inc. Coated enzyme-containing granule
US5324649A (en) 1991-10-07 1994-06-28 Genencor International, Inc. Enzyme-containing granules coated with hydrolyzed polyvinyl alcohol or copolymer thereof
CA2088230A1 (en) 1992-02-03 1993-08-04 James Gordon Detergent composition
GB9205894D0 (en) 1992-03-17 1992-04-29 Unilever Plc Detergent compositions
EP0561452A1 (en) 1992-03-20 1993-09-22 Unilever N.V. Machine dishwashing composition containing polyaminoacids as builders
DK72992D0 (da) 1992-06-01 1992-06-01 Novo Nordisk As Enzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
KR100294361B1 (ko) 1992-07-23 2001-09-17 피아 스타르 돌연변이체알파-아밀라제,세정제,접시세척제,및액화제
US5281526A (en) 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
PL310326A1 (en) 1993-02-11 1995-12-11 Genencor Int Novel oxidation-stable mutants of alpha-amylase as well as detergent and starch liquefaction compositions containing them
CA2138519C (en) 1993-04-27 2007-06-12 Jan Metske Van Der Laan New lipase variants for use in detergent applications
DK52393D0 (da) 1993-05-05 1993-05-05 Novo Nordisk As
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
CA2173946A1 (en) 1993-10-13 1995-04-20 Anders Hjelholt Pedersen H2o2-stable peroxidase variants
DK131193D0 (da) 1993-11-23 1993-11-23 Novo Nordisk As
JPH07143883A (ja) 1993-11-24 1995-06-06 Showa Denko Kk リパーゼ遺伝子及び変異体リパーゼ
MY111537A (en) 1994-02-02 2000-07-31 Novo Nordisk As A combined desizing and bleaching process.
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
ATE222604T1 (de) 1994-02-22 2002-09-15 Novozymes As Methode zur herstellung einer variante eines lipolytischen enzymes
US5824531A (en) 1994-03-29 1998-10-20 Novid Nordisk Alkaline bacilus amylase
JP3851656B2 (ja) 1994-05-04 2006-11-29 ジェネンコア インターナショナル インコーポレーテッド 改善された界面活性剤耐性を有するリパーゼ
AU2884595A (en) 1994-06-20 1996-01-15 Unilever Plc Modified pseudomonas lipases and their use
WO1996000292A1 (en) 1994-06-23 1996-01-04 Unilever N.V. Modified pseudomonas lipases and their use
BE1008998A3 (fr) 1994-10-14 1996-10-01 Solvay Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci.
EP0785994A1 (en) 1994-10-26 1997-07-30 Novo Nordisk A/S An enzyme with lipolytic activity
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
KR19980702782A (ko) 1995-03-09 1998-08-05 혼 마가렛 에이. 녹말 액화 방법
US5736499A (en) 1995-06-06 1998-04-07 Genencor International, Inc. Mutant A-amylase
JP3025627B2 (ja) 1995-06-14 2000-03-27 花王株式会社 液化型アルカリα−アミラーゼ遺伝子
EP0839186B1 (en) 1995-07-14 2004-11-10 Novozymes A/S A modified enzyme with lipolytic activity
AU6513096A (en) 1995-07-19 1997-02-18 Novo Nordisk A/S Treatment of fabrics
DE69632538T2 (de) 1995-08-11 2005-05-19 Novozymes A/S Neuartige lipolytische enzyme
WO1997007205A1 (en) 1995-08-11 1997-02-27 Novo Nordisk A/S Method for preparing polypeptide variants
WO1997041213A1 (en) 1996-04-30 1997-11-06 Novo Nordisk A/S α-AMYLASE MUTANTS
US5763385A (en) 1996-05-14 1998-06-09 Genencor International, Inc. Modified α-amylases having altered calcium binding properties
US5958739A (en) 1996-06-06 1999-09-28 Genencor International Inc. Mutant α-amylase
DE69718351T2 (de) 1996-10-08 2003-11-20 Novozymes As Diaminobenzoesäure derivate als farbstoffvorläufer
EP1001736A1 (en) 1996-10-11 2000-05-24 Novo Nordisk A/S STARCH BINDING DOMAINS (SBDs) FOR ORAL CARE PRODUCTS
CA2270593C (en) 1996-11-04 2005-06-07 Novo Nordisk A/S Subtilase variants and compositions
KR100591553B1 (ko) 1996-11-04 2006-06-19 노보자임스 에이/에스 섭틸라제 변종과 조성물
CA2763810C (en) 1996-11-26 2013-04-02 The Governing Council Of The University Of Toronto Chemically modified subtilisin mutants
US6159731A (en) 1997-02-12 2000-12-12 Massachusetts Institute Of Technology Daxx, a Fas-binding protein that activates JNK and apoptosis
US6361989B1 (en) 1997-10-13 2002-03-26 Novozymes A/S α-amylase and α-amylase variants
JP4358431B2 (ja) 1997-10-13 2009-11-04 ノボザイムス アクティーゼルスカブ α−アミラーゼ変異体
MA25044A1 (fr) 1997-10-23 2000-10-01 Procter & Gamble Compositions de lavage contenant des variants de proteases multisubstituees.
US6562612B2 (en) 1997-11-19 2003-05-13 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
AU748986B2 (en) 1998-04-01 2002-06-13 Dsm N.V. Application of phytase in feed having low content of phytate
US6017867A (en) 1998-06-05 2000-01-25 The Procter & Gamble Company Detergent compositions containing percarbonate and making processes thereof
US6403355B1 (en) 1998-12-21 2002-06-11 Kao Corporation Amylases
JP4745503B2 (ja) 1999-03-31 2011-08-10 ノボザイムス アクティーゼルスカブ アルカリα−アミラーゼ活性を有するポリペプチド及びそれらをコードする核酸
WO2001012792A1 (en) 1999-08-13 2001-02-22 The Victoria University Of Manchester Phytase enzymes, nucleic acids encoding phytase enzymes and vectors and host cells incorporating same
US20020155574A1 (en) 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
EP1309677B2 (en) 2000-08-11 2012-04-11 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
JP4426716B2 (ja) 2000-10-11 2010-03-03 花王株式会社 高生産性α−アミラーゼ
EP2159279A3 (en) 2001-05-15 2010-05-12 Novozymes A/S Alpha-amylase variant with altered properties
ES2245621T3 (es) * 2003-03-10 2010-05-19 Novozymes A/S Procesos para un producto alcoholico.
ATE510925T1 (de) 2003-06-25 2011-06-15 Novozymes As Stärkehydrolyseverfahren
WO2005056783A1 (en) 2003-12-05 2005-06-23 Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Catalytic domains of beta(1,4)-galactosyltransferase i having altered metal ion specificity
BRPI0509671A (pt) 2004-04-08 2007-10-30 Genencor Internation Inc alfa-amilases mutante
CA2854912A1 (en) 2004-07-05 2006-01-12 Novozymes A/S Alpha-amylase variants with altered properties
DE102004047776B4 (de) 2004-10-01 2018-05-09 Basf Se Gegen Di- und/oder Multimerisierung stabilisierte Alpha-Amylase-Varianten, Verfahren zu deren Herstellung sowie deren Verwendung
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
RU2433182C2 (ru) 2005-10-12 2011-11-10 Джененкор Интернэшнл, Инк. Применение и получение стабильной при хранении нейтральной металлопротеиназы
DE102006038448A1 (de) 2005-12-28 2008-02-21 Henkel Kgaa Enzym-haltiges Reinigungsmittel
US20080220498A1 (en) 2007-03-06 2008-09-11 Cervin Marguerite A Variant Buttiauxella sp. phytases having altered properties
MX2009009378A (es) * 2007-03-09 2009-09-22 Danisco Us Inc Genencor Div Variantes de alfa-amilasa de especies de bacillus alcalifilico, composiciones que comprenden las variantes de alfa-amilasa, y metodos de uso.
US20100233780A1 (en) 2007-06-06 2010-09-16 Wolfgang Aehle Methods for Improving Protein Properties
JP2011505121A (ja) 2007-11-05 2011-02-24 ダニスコ・ユーエス・インク 改変された性質を有するアルファ−アミラーゼ
BRPI0820500A2 (pt) 2007-11-05 2015-06-16 Danisco Us Inc Variantes de alfa-amilase de bacillus sp. Ts-23 com propriedades alteradas
EP2212410B1 (en) 2007-11-05 2016-08-10 Danisco US Inc. Variants of bacillus licheniformis alpha-amylase with increased thermostability and/or decreased calcium dependence
KR20100109945A (ko) 2008-02-04 2010-10-11 다니스코 유에스 인크. 변경된 특성을 지닌 ｔｓ23 알파-아밀라아제 변이체
DK2447361T3 (da) 2008-06-06 2015-01-05 Danisco Us Inc Alpha-amylase- (AMYS) varianter af geobacillus stearothermophilus med forbedrede egenskaber

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US8507243B2 (en) 2013-08-13
MX2011003178A (es) 2011-04-21
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