DK2319921T3 - Ny protease og anvendelse af denne - Google Patents
Ny protease og anvendelse af denne Download PDFInfo
- Publication number
- DK2319921T3 DK2319921T3 DK09803009.1T DK09803009T DK2319921T3 DK 2319921 T3 DK2319921 T3 DK 2319921T3 DK 09803009 T DK09803009 T DK 09803009T DK 2319921 T3 DK2319921 T3 DK 2319921T3
- Authority
- DK
- Denmark
- Prior art keywords
- protease
- gly
- ala
- ser
- val
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
Claims (11)
1. Modent protein, der har proteaseaktivitet og består af aminosyresekven-sen med SEQ ID NO: 1 eller NO. 3.
2. Modent protein, der har proteaseaktivitet ifølge krav 1, hvor den optimale temperatur for proteasen er 100 °C eller højere i det tilfælde, hvor der udføres en reaktion af proteasen under anvendelse af azocasein som et substrat ved en pH-værdi på 7 i 20 minutter.
3. Modent protein, der har proteaseaktivitet ifølge krav 1, hvor proteasens restaktivitet er 40 % eller mere, efter at proteasen er behandlet i 50 mM Tris-HCI (pH 7) ved 100 °C i 90 minutter.
4. Modent protein, der har proteaseaktivitet ifølge krav 1, hvor proteasens restaktivitet er 80 % eller mere, efter at proteasen er behandlet i 20 mM Tris-HCI (pH 8) indeholdende 5 % natriumdodecylsulfat ved 55 °C i 60 minutter.
5. Modent protein, der har proteaseaktivitet ifølge krav 1, hvor proteasens Km-værdi er 0,1 til 1 nM i det tilfælde, hvor der udføres en reaktion af proteasen under anvendelse af Suc-AAPF-pNA som et substrat ved 80 °C.
6. Pro-form af det modne protein, der har proteaseaktivitet ifølge et af kravene 1 til 5, bestående af den følgende aminosyresekvens: aminosyresekvensen med SEQ ID NO: 5.
7. Polynukleotid, der koder det modne protein, der har proteaseaktivitet ifølge et af kravene 1 til 5, bestående af en hvilken som helst af basissekvenserne med SEQ ID NO: 2 eller 4.
8. Polynukleotid, der koder pro-formen ifølge krav 6, hvilket består af basissekvensen med SEQ ID NO:6.
9. Ekspressionsvektor omfattende polynukleotidet ifølge et af krav 7 eller 8.
10. Transformant, til hvilken ekspressionsvektoren ifølge krav 9 er overført.
11. Rensemiddel omfattende det modne protein, der har proteaseaktivitet ifølge et af kravene 1 til 5.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008197467 | 2008-07-31 | ||
| PCT/JP2009/063547 WO2010013767A1 (ja) | 2008-07-31 | 2009-07-30 | 新規なプロテアーゼおよびその利用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2319921T3 true DK2319921T3 (da) | 2016-06-27 |
Family
ID=41610466
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK09803009.1T DK2319921T3 (da) | 2008-07-31 | 2009-07-30 | Ny protease og anvendelse af denne |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8535928B2 (da) |
| EP (1) | EP2319921B1 (da) |
| JP (1) | JP5339543B2 (da) |
| CN (1) | CN102112611B (da) |
| DK (1) | DK2319921T3 (da) |
| WO (1) | WO2010013767A1 (da) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2744900B1 (en) * | 2011-08-19 | 2017-07-19 | Novozymes A/S | Polypeptides having protease activity |
| CN104884598B (zh) * | 2012-12-20 | 2019-04-09 | 荷兰联合利华有限公司 | 酶处理组合物 |
| WO2016196202A1 (en) * | 2015-05-29 | 2016-12-08 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5643777A (en) * | 1990-06-15 | 1997-07-01 | Novo Nordisk A/S | Thermostable protease from thermococcus |
| CA2238296C (en) | 1995-12-12 | 2008-07-22 | Takara Shuzo Co., Ltd. | Ultrathermostable protease genes |
| DE69833652T2 (de) * | 1997-06-10 | 2006-09-21 | Takara Bio Inc., Otsu | System zum exprimieren einer hyperthermostabilen protease |
| CA2497338A1 (en) | 2002-08-30 | 2004-03-18 | Japan Science And Technology Corporation | Method of targeted gene disruption, genome of hyperthermostable bacterium and genome chip using the same |
| JP2007006846A (ja) * | 2005-07-01 | 2007-01-18 | Kyoto Univ | 耐熱性プロテアーゼ |
-
2009
- 2009-07-30 US US13/055,828 patent/US8535928B2/en active Active
- 2009-07-30 DK DK09803009.1T patent/DK2319921T3/da active
- 2009-07-30 WO PCT/JP2009/063547 patent/WO2010013767A1/ja active Application Filing
- 2009-07-30 CN CN2009801304904A patent/CN102112611B/zh not_active Expired - Fee Related
- 2009-07-30 JP JP2010522747A patent/JP5339543B2/ja active Active
- 2009-07-30 EP EP09803009.1A patent/EP2319921B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010013767A1 (ja) | 2010-02-04 |
| US20110143421A1 (en) | 2011-06-16 |
| EP2319921A4 (en) | 2012-05-16 |
| CN102112611A (zh) | 2011-06-29 |
| US8535928B2 (en) | 2013-09-17 |
| EP2319921A1 (en) | 2011-05-11 |
| JPWO2010013767A1 (ja) | 2012-01-12 |
| JP5339543B2 (ja) | 2013-11-13 |
| EP2319921B1 (en) | 2016-04-20 |
| CN102112611B (zh) | 2013-07-24 |
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