WO2010013767A1 - 新規なプロテアーゼおよびその利用 - Google Patents

新規なプロテアーゼおよびその利用 Download PDF

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Publication number
WO2010013767A1
WO2010013767A1 PCT/JP2009/063547 JP2009063547W WO2010013767A1 WO 2010013767 A1 WO2010013767 A1 WO 2010013767A1 JP 2009063547 W JP2009063547 W JP 2009063547W WO 2010013767 A1 WO2010013767 A1 WO 2010013767A1
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
acid sequence
seq
sequence depicted
protease
Prior art date
Application number
PCT/JP2009/063547
Other languages
English (en)
French (fr)
Inventor
茂則 金谷
チタ フーパオ
和文 高野
古賀　雄一
Original Assignee
国立大学法人大阪大学
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 国立大学法人大阪大学 filed Critical 国立大学法人大阪大学
Priority to US13/055,828 priority Critical patent/US8535928B2/en
Priority to JP2010522747A priority patent/JP5339543B2/ja
Priority to DK09803009.1T priority patent/DK2319921T3/en
Priority to EP09803009.1A priority patent/EP2319921B1/en
Priority to CN2009801304904A priority patent/CN102112611B/zh
Publication of WO2010013767A1 publication Critical patent/WO2010013767A1/ja

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea

Abstract

　（ａ）配列番号１に示されるアミノ酸配列、（ｂ）配列番号１に示されるアミノ酸配列において１もしくは数個のアミノ酸が欠失、置換もしくは付加されたアミノ酸配列、（ｃ）配列番号３に示されるアミノ酸配列、または、（ｄ）配列番号３に示されるアミノ酸配列において１もしくは数個のアミノ酸が欠失、置換もしくは付加されたアミノ酸配列からなる新規なプロテアーゼは、高温かつ高アルカリ条件下で高い活性を有し、タンパク質変性剤や界面活性剤に対する安定性が高く、洗剤用プロテアーゼとして高い有用性を有する。
PCT/JP2009/063547 2008-07-31 2009-07-30 新規なプロテアーゼおよびその利用 WO2010013767A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US13/055,828 US8535928B2 (en) 2008-07-31 2009-07-30 Protease and use thereof
JP2010522747A JP5339543B2 (ja) 2008-07-31 2009-07-30 新規なプロテアーゼおよびその利用
DK09803009.1T DK2319921T3 (en) 2008-07-31 2009-07-30 New protease and use of this
EP09803009.1A EP2319921B1 (en) 2008-07-31 2009-07-30 Novel protease and use thereof
CN2009801304904A CN102112611B (zh) 2008-07-31 2009-07-30 新型蛋白酶及其应用

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2008197467 2008-07-31
JP2008-197467 2008-07-31

Publications (1)

Publication Number Publication Date
WO2010013767A1 true WO2010013767A1 (ja) 2010-02-04

Family

ID=41610466

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2009/063547 WO2010013767A1 (ja) 2008-07-31 2009-07-30 新規なプロテアーゼおよびその利用

Country Status (6)

Country Link
US (1) US8535928B2 (ja)
EP (1) EP2319921B1 (ja)
JP (1) JP5339543B2 (ja)
CN (1) CN102112611B (ja)
DK (1) DK2319921T3 (ja)
WO (1) WO2010013767A1 (ja)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2744900B1 (en) * 2011-08-19 2017-07-19 Novozymes A/S Polypeptides having protease activity
CN104884598B (zh) * 2012-12-20 2019-04-09 荷兰联合利华有限公司 酶处理组合物
WO2016196202A1 (en) * 2015-05-29 2016-12-08 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997021823A1 (fr) * 1995-12-12 1997-06-19 Takara Shuzo Co., Ltd. Genes de proteases ultrathermostables
WO1998056926A1 (fr) * 1997-06-10 1998-12-17 Takara Shuzo Co., Ltd. Systeme pour exprimer une proteine hyperthermostable
JP2007006846A (ja) * 2005-07-01 2007-01-18 Kyoto Univ 耐熱性プロテアーゼ

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5643777A (en) * 1990-06-15 1997-07-01 Novo Nordisk A/S Thermostable protease from thermococcus
CA2497338A1 (en) 2002-08-30 2004-03-18 Japan Science And Technology Corporation Method of targeted gene disruption, genome of hyperthermostable bacterium and genome chip using the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997021823A1 (fr) * 1995-12-12 1997-06-19 Takara Shuzo Co., Ltd. Genes de proteases ultrathermostables
WO1998056926A1 (fr) * 1997-06-10 1998-12-17 Takara Shuzo Co., Ltd. Systeme pour exprimer une proteine hyperthermostable
JP2007006846A (ja) * 2005-07-01 2007-01-18 Kyoto Univ 耐熱性プロテアーゼ

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK [online] 31 October 2006 (2006-10-31), FUKUI T. ET AL.: "Definition: Subtilisin-like serine protease.", XP008144087, Database accession no. Q5JIZ5 *
FUKUI T ET AL., GENOME RES, vol. 15, no. 3, March 2005 (2005-03-01), pages 352 - 63
HARLOW ET AL.: "Antibodies: a laboratory manual", 1988, COLD SPRING HARBOR LABORATORY
IWASAKI ET AL.: "Monoclonal antibody, hybridoma and ELISA", 1991, KODANSHA LTD.
KANNAN, Y.; KOGA, Y.; INOUE, Y.; HARUKI, M.; TAKAGI, M.; IMANAKA, T. ET AL.: "Active subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence", APPL. ENVIRON. MICROBIOL., vol. 67, 2001, pages 2445 - 2552
MORIKAWA M ET AL., APPL ENVIRON MICROBIOL, vol. 60, no. 12, December 1994 (1994-12-01), pages 4559 - 66
MORIKAWA. ET AL., APPL. ENVIRON. MICROBIOL., vol. 60, 1994, pages 4559 - 4566
PULIDO, M.; SAITO, K.; TANAKA, S.; KOGA, Y.; TAKANO, K.; KANAYA, S.: "Ca2+-dependent maturation of Tksubtilisin from a hyperthermophilic archaeon: propeptide is a potent inhibitor of the mature domain but is not required for its folding", APPL. ENVIRON. MICROBIOL., vol. 72, 2006, pages 4154 - 4162
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY
See also references of EP2319921A4
YUKI ISHIDA ET AL.: "Chokonetsu Kosaikin Yurai Tk-subtilisin no Seijukuka no Netsuyuhatsu Kiko", DAI 58 KAI ABSTRACTS OF THE ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, - 3 August 2006 (2006-08-03), pages 118, XP008144109 *
YUKI TAKEUCHI ET AL.: "Chokonetsu Kosaikin (Shigenkin) Yurai Subtilisin ni Ketsugo suru Calcium Ion no Yakuwari", DAI 7 KAI ANNUAL MEETING OF THE PROTEIN SCIENCE SOCIETY OF JAPAN PROGRAM-YOSHISHU, - 7 May 2007 (2007-05-07), pages 131, XP008144117 *

Also Published As

Publication number Publication date
US20110143421A1 (en) 2011-06-16
EP2319921A4 (en) 2012-05-16
CN102112611A (zh) 2011-06-29
US8535928B2 (en) 2013-09-17
EP2319921A1 (en) 2011-05-11
JPWO2010013767A1 (ja) 2012-01-12
DK2319921T3 (en) 2016-06-27
JP5339543B2 (ja) 2013-11-13
EP2319921B1 (en) 2016-04-20
CN102112611B (zh) 2013-07-24

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