DK2296672T3 - Konditioneret medium af leverforløberceller - Google Patents
Konditioneret medium af leverforløberceller Download PDFInfo
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- DK2296672T3 DK2296672T3 DK09761761.7T DK09761761T DK2296672T3 DK 2296672 T3 DK2296672 T3 DK 2296672T3 DK 09761761 T DK09761761 T DK 09761761T DK 2296672 T3 DK2296672 T3 DK 2296672T3
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Claims (15)
1. Fremgangsmåde til fremstilling af et konditioneret medium, omfattende trinene: (i) dyrkning af fuldt udviklede leverafledede humane modne hepatocytter i et celledyrkningsmedium indtil død af modne hepatocytter og udvælgelse af en population af overlevende celler med epiteloid morfologi, (ii) ekspandering af populationen af overlevende celler med epiteloid morfologi ved dyrkning i et serumholdigt, glukoseholdigt dyrkningsmedium suppleret med hEGF (human epitelial vækstfaktor) og bFGF (basal fibroblast vækstfaktor), og omfattende almindelige uorganiske salte, aminosyrer og vitaminer nødvendige for væksten af pattedyrceller, og (iii) udskillelse af cellerne fra celledyrkningsmediet.
2. Fremgangsmåde ifølge krav 1, hvori de modne hepatocytter fryses i et serumholdigt dyrkningsmedium under tilstedeværelse af et kuldebeskyttende middel og derefter optøs før dyrkning ifølge trin (i).
3. Fremgangsmåde ifølge krav 1 eller 2, hvori dyrkningsmediet omfatter alpha-MEM suppleret med humanalbumin af GMP-kvalitet.
4. Konditioneret medium, der kan fremskaffes ved dyrkning af en pluripotent forløbercellelinie af human lever, kendetegnet ved, at det er frit for celler og yderligere kendetegnet ved, at det omfatter en blanding af mindst hepatocytvækstfak-tor (FHGF), interleukin 6 (IL-6), interleukin 8 (IL-8) og vaskulær endotelial vækstfaktor (VEGF).
5. Konditioneret mediumsammensætning ifølge krav 4, som kan fremskaffes ved fremgangsmåden ifølge ethvert af krav 1 til 3.
6. Farmaceutisk sammensætning omfattende en farmaceutisk effektiv mængde af det konditionerede medium ifølge krav 4 eller 5.
7. Farmaceutisk sammensætning omfattende en farmaceutisk effektiv mængde af en blanding af mindst hepatocytvækstfaktor (FIGF), interleukin 6 (IL-6), interleukin 8 (IL-8) og vaskulær endotelial vækstfaktor (VEGF) i en koncentration i området fra 10 til 400 ng/ml.
8. Farmaceutisk sammensætning ifølge krav 7, hvori blandingen yderligere omfatter makrofagstimulerende protein (MSP) i en farmaceutisk aktiv mængde.
9. Farmaceutisk sammensætning ifølge krav 7 eller 8, hvori hepatocytvækstfakto-ren (HGF) er i en koncentration fra 1 til 100 ng/ml, interleukin 6 (IL-6) er i en koncentration fra 10 til 200 ng/ml, interleukin 8 (IL-8) er i en koncentration lig med eller højere end 35 ng/ml.
10. Farmaceutisk sammensætning ifølge krav 8 eller 9, hvori det makrofagstimulerende protein (MSP) er i en koncentration i området fra 1 til 100 pg/ml.
11. Koncentreret farmaceutisk sammensætning bestående af den farmaceutiske sammensætning ifølge krav 9 eller 10 koncentreret mindst 5 gange, fortrinsvis mindst 10 gange, mere foretrukket mindst 20 gange, endda mere foretrukket mindst 25 gange.
12. Fortyndet farmaceutisk sammensætning bestående af den farmaceutiske sammensætning ifølge krav 9 eller 10 fortyndet mindst 5 gange, fortrinsvis mindst 10 gange, mere foretrukket mindst 20 gange, endda mere foretrukket mindst 25 gange.
13. Farmaceutisk sammensætning ifølge ethvert af krav 6 til 12 til anvendelse ved behandling af organskade eller -svigt.
14. Farmaceutisk sammensætning til anvendelse ifølge krav 13, hvori organet er lever eller nyre.
15. Farmaceutisk sammensætning til anvendelse ifølge krav 13 eller 14, som er fremstillet som et medikament egnet til at indgive en dosis hepatocytvækstfaktor (HGF) i området 0,01 til 1 mg/kg, en dosis interleukin 6 (IL-6) i området 0,01 til 1 mg/kg, en dosis interleukin 9 (IL-8) i området 0,01 til 1 mg/kg og valgfrit en dosis vaskulær endotelial vækstfaktor (VEGF) i området mellem 0,01 til 1 mg/kg.
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| EP08010651 | 2008-06-11 | ||
| PCT/EP2009/057232 WO2009150199A1 (en) | 2008-06-11 | 2009-06-11 | Conditioned medium of liver progenitor cells |
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| DK2296672T3 true DK2296672T3 (da) | 2016-01-04 |
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| CN (2) | CN103800895B (da) |
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| CA (1) | CA2727392C (da) |
| DK (1) | DK2296672T3 (da) |
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| HU (1) | HUE028676T2 (da) |
| PL (1) | PL2296672T3 (da) |
| PT (1) | PT2296672E (da) |
| WO (1) | WO2009150199A1 (da) |
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| EP2333047A1 (en) * | 2009-12-09 | 2011-06-15 | Fresenius Medical Care Deutschland GmbH | Adult stem cell derived conditioned medium and/or adult stem cells for use in the therapeutic treatment of a tumor disease |
| US8603470B1 (en) | 2012-08-07 | 2013-12-10 | National Cheng Kung University | Use of IL-20 antagonists for treating liver diseases |
| JP6474394B2 (ja) * | 2013-07-05 | 2019-02-27 | ユニベルシテ カトリック ドゥ ルーベン | ヒト成体肝臓幹細胞由来の馴化培地および肝臓障害の治療におけるその使用 |
| AR099641A1 (es) | 2013-09-05 | 2016-08-10 | Consejo Nac De Investig Científicas Y Técnicas (Conicet) | OSTEONECTINA (SPARC) COMO BLANCO TERAPÉUTICO PARA EL TRATAMIENTO Y PREVENCIÓN DEL FALLO HEPÁTICO AGUDO, COMPOSICIÓN FARMACEUTICA, CONSTRUCTO GENÉTICO, ARNsi, AGENTE QUE INHIBE LA EXPRESIÓN, USO |
| RU2644650C2 (ru) * | 2014-12-01 | 2018-02-13 | Общество с ограниченной ответственностью "Т-Хелпер Клеточные Технологии" | Материал стволовых клеток и способ его получения |
| CN104818245B (zh) * | 2015-05-29 | 2020-02-04 | 广州赛莱拉干细胞科技股份有限公司 | 一种肝脏干细胞的培养基及培养方法 |
| KR20180108789A (ko) | 2016-02-10 | 2018-10-04 | 웨이크 포리스트 유니버시티 헬스 사이언시즈 | 간 섬유화의 모델 시스템 및 그의 제조 및 사용 방법 |
| RU2708329C2 (ru) | 2016-05-31 | 2019-12-05 | Общество с ограниченной ответственностью "Т-Хелпер Клеточные Технологии" | Материал стволовых клеток, композиции и способы применения |
| CN109628377A (zh) * | 2019-01-02 | 2019-04-16 | 贵州省人民医院 | 一种小鼠原代肝细胞灌注式分离及离体培养方法 |
| CN111607556B (zh) * | 2019-01-25 | 2022-06-07 | 中国科学院广州生物医药与健康研究院 | 一种培养扩增人肝祖细胞的培养基及其应用 |
| TW202106875A (zh) * | 2019-04-30 | 2021-02-16 | 比利時商普羅米修亞生物科技股份有限公司 | 人類同種異體肝衍生前驅細胞的製備(二) |
| CN111394391B (zh) * | 2019-07-11 | 2022-12-06 | 上海赛立维生物科技有限公司 | 肝祖细胞样细胞库的构建方法及其制备的细胞株与应用 |
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| JPH093099A (ja) * | 1995-06-20 | 1997-01-07 | Toyobo Co Ltd | マクロファージ刺激蛋白質改変体およびその製造法 |
| US6475490B1 (en) * | 1998-10-19 | 2002-11-05 | Fordham University | Compositions and methods for promoting tissue repair using heat shock proteins |
| US6372494B1 (en) * | 1999-05-14 | 2002-04-16 | Advanced Tissue Sciences, Inc. | Methods of making conditioned cell culture medium compositions |
| US6719969B1 (en) * | 1999-08-09 | 2004-04-13 | The Regents Of The University Of Michigan | Treatment of liver disease and injury with CXC chemokines |
| CA2290240C (en) | 1999-11-24 | 2008-03-11 | Stergios Stergiopoulos | High resolution 3d ultrasound imaging system deploying a multi-dimensional array of sensors and method for multi-dimensional beamforming sensor signals |
| US7547674B2 (en) * | 2001-06-06 | 2009-06-16 | New York Medical College | Methods and compositions for the repair and/or regeneration of damaged myocardium |
| US6916770B2 (en) | 2001-04-27 | 2005-07-12 | Polyone Corporation | Multi-functional color concentrate compositions |
| WO2002098365A2 (en) * | 2001-06-07 | 2002-12-12 | Skinmedica, Inc. | Conditioned cell culture media and uses thereof |
| WO2003033697A1 (en) * | 2001-10-18 | 2003-04-24 | Ixion Biotechnology, Inc. | Conversion of liver stem and progenitor cells to pancreatic functional cells |
| WO2006126219A1 (en) * | 2005-05-26 | 2006-11-30 | Fresenius Medical Care Deutschland G.M.B.H. | Liver progenitor cells |
| WO2007097993A2 (en) * | 2006-02-16 | 2007-08-30 | The Burnham Institute Of Medical Research | Media conditioned by human embryonic stem cells or other progenitor cells and uses therefor |
| KR101505202B1 (ko) * | 2006-03-07 | 2015-03-27 | 지타 쉬로프 | 인간 배아 줄기 세포 및 그의 유도체를 포함하는 조성물, 그의 사용 방법 및 제조 방법 |
| FR2912916B1 (fr) * | 2007-02-26 | 2009-05-22 | Oreal | Composition cosmetique ou dermatologique comprenant un milieu de culture cellulaire |
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- 2009-06-11 EP EP09761761.7A patent/EP2296672B1/en active Active
- 2009-06-11 US US12/737,143 patent/US10456425B2/en active Active
- 2009-06-11 WO PCT/EP2009/057232 patent/WO2009150199A1/en active Application Filing
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Also Published As
| Publication number | Publication date |
|---|---|
| CA2727392C (en) | 2017-08-22 |
| US20170182100A1 (en) | 2017-06-29 |
| CN103800895A (zh) | 2014-05-21 |
| ES2556630T3 (es) | 2016-01-19 |
| US10357519B2 (en) | 2019-07-23 |
| EP2296672B1 (en) | 2015-09-16 |
| HUE028676T2 (en) | 2016-12-28 |
| EP2296672A1 (en) | 2011-03-23 |
| BRPI0909866A2 (pt) | 2015-08-11 |
| BRPI0909866B1 (pt) | 2021-08-24 |
| PL2296672T3 (pl) | 2016-03-31 |
| PT2296672E (pt) | 2016-01-22 |
| US20110129439A1 (en) | 2011-06-02 |
| US10456425B2 (en) | 2019-10-29 |
| CN102119031A (zh) | 2011-07-06 |
| JP5632833B2 (ja) | 2014-11-26 |
| CN102119031B (zh) | 2014-09-10 |
| JP2011522553A (ja) | 2011-08-04 |
| CA2727392A1 (en) | 2009-12-17 |
| CN103800895B (zh) | 2016-12-28 |
| WO2009150199A1 (en) | 2009-12-17 |
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