DK2115120T3 - Mikroorganisme der producerer inosin og fremgangsmåde til fremstilling af inosin under anvendelse af samme - Google Patents

Mikroorganisme der producerer inosin og fremgangsmåde til fremstilling af inosin under anvendelse af samme Download PDF

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Publication number
DK2115120T3
DK2115120T3 DK08704775.9T DK08704775T DK2115120T3 DK 2115120 T3 DK2115120 T3 DK 2115120T3 DK 08704775 T DK08704775 T DK 08704775T DK 2115120 T3 DK2115120 T3 DK 2115120T3
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DK
Denmark
Prior art keywords
gene
corynebacterium ammoniagenes
inosine
rih
seq
Prior art date
Application number
DK08704775.9T
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English (en)
Inventor
Young-Hoon Park
Hee-Jong Lee
Jin-Nam Lee
Kwang-Myung Cho
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Cj Cheiljedang Corp
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Publication of DK2115120T3 publication Critical patent/DK2115120T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B28—WORKING CEMENT, CLAY, OR STONE
    • B28D—WORKING STONE OR STONE-LIKE MATERIALS
    • B28D1/00—Working stone or stone-like materials, e.g. brick, concrete or glass, not provided for elsewhere; Machines, devices, tools therefor
    • B28D1/02—Working stone or stone-like materials, e.g. brick, concrete or glass, not provided for elsewhere; Machines, devices, tools therefor by sawing
    • B28D1/04—Working stone or stone-like materials, e.g. brick, concrete or glass, not provided for elsewhere; Machines, devices, tools therefor by sawing with circular or cylindrical saw-blades or saw-discs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B28—WORKING CEMENT, CLAY, OR STONE
    • B28D—WORKING STONE OR STONE-LIKE MATERIALS
    • B28D7/00—Accessories specially adapted for use with machines or devices of the preceding groups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C12P19/40—Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
    • C12Y302/02008—Ribosylpyrimidine nucleosidase (3.2.2.8)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Mechanical Engineering (AREA)
  • Mining & Mineral Resources (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (5)

1. Rekombinant Corynebacterium ammoniagenes der producerer inosin, hvori genet der koder for nukleosidhydrolase II med nukleotidsekvensen repræsenteret ved SEQ. ID. NO: 1 er inaktiveret og hvori genet der koder for 5'-nukleotidase med nukleotidsekvensen repræsenteret ved SEQ ID NO:2 er over-udtrykt.
2. Rekombinant Corynebacterium ammoniagenes ifølge krav 1, hvor inaktiveringen er blevet induceret ved transformation afen Corynebacterium ammoniagenes med en vektor omfattende en del af sekvensen repræsenteret ved SEQ. ID. NO: 1 og en antibiotisk markør.
3. Rekombinant Corynebacterium ammoniagenes ifølge krav 1 eller krav 2, hvor over-udtryk af genet er blevet induceret ved transformation af en Corynebacterium ammoniagenes med en vektor indeholdende sekvensen repræsenteret ved SEQ. ID. NO: 2.
4. Rekombinant Corynebacterium ammoniagenes ifølge et hvilket som helst af kravene 1 til 3, hvor den rekombinante Corynebacterium ammoniagenes er Corynebacterium ammoniagenes CJIG651 KCCM-10829P.
5. Fremgangsmåde til fremstilling af inosin ved at dyrke den rekombinante Corynebacterium ammoniagenes ifølge et hvilket som helst af kravene 1 til 4 og opnå det akkumulerede inosin fra dyrkningsopløsningen.
DK08704775.9T 2007-01-15 2008-01-15 Mikroorganisme der producerer inosin og fremgangsmåde til fremstilling af inosin under anvendelse af samme DK2115120T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020070004341A KR100882418B1 (ko) 2007-01-15 2007-01-15 이노신을 생산하는 미생물 및 그를 이용한 이노신 제조방법
PCT/KR2008/000236 WO2008088156A1 (en) 2007-01-15 2008-01-15 Microorganism producing inosine and method of producing inosine using the same

Publications (1)

Publication Number Publication Date
DK2115120T3 true DK2115120T3 (da) 2016-12-05

Family

ID=39636124

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08704775.9T DK2115120T3 (da) 2007-01-15 2008-01-15 Mikroorganisme der producerer inosin og fremgangsmåde til fremstilling af inosin under anvendelse af samme

Country Status (7)

Country Link
US (2) US8168422B2 (da)
EP (1) EP2115120B1 (da)
KR (1) KR100882418B1 (da)
CN (1) CN101583709B (da)
DK (1) DK2115120T3 (da)
ES (1) ES2601513T3 (da)
WO (1) WO2008088156A1 (da)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100882418B1 (ko) 2007-01-15 2009-02-05 씨제이제일제당 (주) 이노신을 생산하는 미생물 및 그를 이용한 이노신 제조방법
KR100959662B1 (ko) * 2008-01-04 2010-05-26 씨제이제일제당 (주) 이노신 생산능을 갖는 코리네박테리움속 미생물 및 이를이용한 이노신의 생산 방법
KR101166027B1 (ko) 2009-04-01 2012-07-19 씨제이제일제당 (주) 5'-이노신산 생산성이 향상된 코리네박테리움 속 미생물 및 이를 이용한 핵산의 생산방법
EP2689014B1 (en) 2011-03-24 2019-04-24 Butamax (TM) Advanced Biofuels LLC Host cells and methods for production of isobutanol
CN103150487B (zh) * 2012-11-29 2016-05-11 扬州大学 一种构建鸡肌肉肌苷酸合成途径相关关键酶基因网络调控方法
KR101904675B1 (ko) * 2017-12-15 2018-10-04 씨제이제일제당 (주) 5'-이노신산을 생산하는 미생물 및 이를 이용한 5'-이노신산의 생산 방법
KR101916611B1 (ko) * 2017-12-15 2018-11-07 씨제이제일제당 (주) 신규 폴리펩타이드 및 이를 이용한 imp 생산방법
KR101916622B1 (ko) * 2018-01-04 2018-11-07 씨제이제일제당 (주) 신규 폴리펩타이드 및 이를 이용한 imp 생산방법
CN113186176B (zh) * 2021-04-22 2023-04-04 华东理工大学 一种全合成培养基及发酵法生产米黑根毛霉脂肪酶的方法

Family Cites Families (10)

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GB1115114A (en) * 1965-07-20 1968-05-29 Kyowa Hakko Kogyo Kk Process for producing inosine
GB1175979A (en) * 1965-12-28 1970-01-01 Takeda Chemical Industries Ltd A method for the Production of 5'-Inosinic Acid and Inosine
KR910001786B1 (en) 1986-07-21 1991-03-23 Mitsumi Electric Co Ltd Circuit board
HU225541B1 (en) 1998-09-25 2007-03-28 Ajinomoto Kk Process for producing l-amino acids by fermentation and amino acid-producing bacterium strains
JP4352716B2 (ja) * 2003-02-17 2009-10-28 味の素株式会社 バチルス属に属するイノシン生産菌及びイノシンの製造法
RU2271391C2 (ru) * 2003-09-03 2006-03-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" СПОСОБ ПОЛУЧЕНИЯ ИНОЗИНА И 5'-ИНОЗИНОВОЙ КИСЛОТЫ МЕТОДОМ ФЕРМЕНТАЦИИ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ, ПРИНАДЛЕЖАЩИХ К РОДУ Escherichia
KR100542568B1 (ko) * 2003-12-10 2006-01-11 씨제이 주식회사 5'-크산틸산을 생산하는 미생물
KR100576341B1 (ko) * 2003-12-15 2006-05-03 씨제이 주식회사 5'-뉴클레오티다제를 코딩하는 유전자가 불활성화된 코리네박테리움 속 미생물 및 이를 이용한 5'-이노신산의 제조방법
KR100694427B1 (ko) * 2005-12-02 2007-03-12 씨제이 주식회사 코리네박테리움 속 미생물 및 이를 이용한 5'-이노신산의제조 방법
KR100882418B1 (ko) 2007-01-15 2009-02-05 씨제이제일제당 (주) 이노신을 생산하는 미생물 및 그를 이용한 이노신 제조방법

Also Published As

Publication number Publication date
KR20080067145A (ko) 2008-07-18
ES2601513T3 (es) 2017-02-15
KR100882418B1 (ko) 2009-02-05
CN101583709B (zh) 2012-07-04
EP2115120B1 (en) 2016-10-05
US20100081173A1 (en) 2010-04-01
EP2115120A1 (en) 2009-11-11
US8187840B2 (en) 2012-05-29
US20110318783A1 (en) 2011-12-29
CN101583709A (zh) 2009-11-18
EP2115120A4 (en) 2010-04-28
WO2008088156A1 (en) 2008-07-24
US8168422B2 (en) 2012-05-01

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