DK2097531T3 - Fremgangsmåder til opnåelse af genetisk kompetence hos bacillus-celler - Google Patents

Fremgangsmåder til opnåelse af genetisk kompetence hos bacillus-celler Download PDF

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Publication number
DK2097531T3
DK2097531T3 DK07865884.6T DK07865884T DK2097531T3 DK 2097531 T3 DK2097531 T3 DK 2097531T3 DK 07865884 T DK07865884 T DK 07865884T DK 2097531 T3 DK2097531 T3 DK 2097531T3
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DK
Denmark
Prior art keywords
getting
procedures
bacillus cells
genetic competence
competence
Prior art date
Application number
DK07865884.6T
Other languages
English (en)
Inventor
Randy Berka
Michelle Maranta
Maria Tang
Barbara Cherry
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of DK2097531T3 publication Critical patent/DK2097531T3/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
DK07865884.6T 2006-12-21 2007-12-19 Fremgangsmåder til opnåelse af genetisk kompetence hos bacillus-celler DK2097531T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US87705306P 2006-12-21 2006-12-21
PCT/US2007/088186 WO2008079895A2 (en) 2006-12-21 2007-12-19 Methods of obtaining genetic competence in bacillus cells

Publications (1)

Publication Number Publication Date
DK2097531T3 true DK2097531T3 (da) 2020-01-02

Family

ID=39563193

Family Applications (1)

Application Number Title Priority Date Filing Date
DK07865884.6T DK2097531T3 (da) 2006-12-21 2007-12-19 Fremgangsmåder til opnåelse af genetisk kompetence hos bacillus-celler

Country Status (5)

Country Link
US (2) US8389283B2 (da)
EP (1) EP2097531B1 (da)
CN (1) CN101657538A (da)
DK (1) DK2097531T3 (da)
WO (1) WO2008079895A2 (da)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2900689B1 (en) * 2012-09-27 2017-08-02 Novozymes, Inc. Bacterial mutants with improved transformation efficiency
DK3019607T4 (da) 2013-07-12 2022-10-17 Novozymes As Direkte overførsel af polynukleotider mellem genomer
WO2017083623A1 (en) 2015-11-12 2017-05-18 Novozymes Bioag A/S Wap genes and proteins that improve bacillus competitiveness
CN105861405A (zh) * 2016-05-06 2016-08-17 中国科学院上海高等研究院 一种高转化率的枯草芽孢杆菌及其构建方法
EP3571217B1 (en) 2017-01-23 2023-06-21 Danisco US Inc. Methods and compositions for obtaining natural competence in bacillus host cells
CN108359659B (zh) * 2018-02-06 2018-12-14 横琴仲泰生物医药有限公司 一种热稳定性高的碱性蛋白酶BmP突变体及其编码基因
CN112062821B (zh) * 2020-09-24 2022-02-01 江南大学 一种碳分解代谢调控蛋白CcpA突变体K31A
EP4225779A2 (en) 2020-10-07 2023-08-16 Basf Se Bacillus cell with reduced lipase and/or esterase side activities
CN112501143B (zh) * 2020-12-15 2022-03-08 安徽工程大学 一种类异戊二烯转移酶ComQ突变体、基因、载体、工程菌及制备方法和应用
CN112921048B (zh) * 2021-03-05 2022-03-08 安徽工程大学 一种纳豆激酶工程菌及提高纳豆激酶活力的方法
CN114292864B (zh) * 2021-12-31 2023-08-08 淮阴工学院 高产表面活性素的贝莱斯芽孢杆菌突变株及其构建方法和应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1309677B2 (en) 2000-08-11 2012-04-11 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
EP1412513B1 (en) * 2001-07-30 2010-04-07 Genencor International, Inc. Phosphate limited inducible promoter and a bacillus expression system

Also Published As

Publication number Publication date
WO2008079895A3 (en) 2008-10-02
US8685738B2 (en) 2014-04-01
EP2097531B1 (en) 2019-09-18
CN101657538A (zh) 2010-02-24
US8389283B2 (en) 2013-03-05
EP2097531A2 (en) 2009-09-09
WO2008079895A2 (en) 2008-07-03
US20100028944A1 (en) 2010-02-04
US20130157309A1 (en) 2013-06-20

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