DK2054432T3 - Specifikke proteiner med høj bindingsaffinitet med modificerede SH3-domæner i FYN-kinase - Google Patents
Specifikke proteiner med høj bindingsaffinitet med modificerede SH3-domæner i FYN-kinase Download PDFInfo
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- DK2054432T3 DK2054432T3 DK07801764.7T DK07801764T DK2054432T3 DK 2054432 T3 DK2054432 T3 DK 2054432T3 DK 07801764 T DK07801764 T DK 07801764T DK 2054432 T3 DK2054432 T3 DK 2054432T3
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
Claims (14)
1. Rekombinant bindingsprotein med specifik bindingsaffinitet til et protein eller et peptid, hvor proteinet eller peptidet ikke er en naturlig SH3-bindende ligand, omfattende mindst ét derivat af Src-homologi-3-domænet (SH3) i FYN-kinase, hvori (a) mindst én aminosyre inde i eller placeret op til to aminosyrer nabostillet til src-sløjfen og (b) mindst én aminosyre inde i eller placeret op til to aminosyrer nabostillet til RT-sløjfen er erstattet, fjernet eller tilføjet, idet SH3-domæne-derivatet har en aminosyresekvens med mindst 70% sekvensidentitet med aminosyresekvensen SEQ ID NO: 1, og idet SH3-domæne-derivatet har mindst 85% identitet med aminosyren i SEQ ID NO: 1, som repræsenterer Src-homologi-3-domænet (SH3) i FYN-kinasen uden for src- og RT-sløjferne, og med det forbehold, at det rekombinante protein ikke er et naturligt SH3-domæne, der indeholder protein, som findes i naturen.
2. Bindingsprotein ifølge krav 1, som omfatter mindst to derivater af SH3-domænet, fortrinsvis et bivalent bindingsprotein.
3. Bindingsprotein ifølge krav 1 eller 2 omfattende én eller fortrinsvis to ændrede rester i stilling 37 og/eller 50 i SH3-domæne-derivatet, fortrinsvis to hydrofobe ændrede rester, mere foretrukket Trp37 og/eller Tyr50, idet Trp37 og Tyr50 især foretrækkes.
4. Bindingsprotein ifølge et hvilket som helst af kravene 1 til 3 med en specifik bindingsaffinitet på 10'7 til 10'12 M, fortrinsvis 10-8 til 10'12 M, til det ekstracellulære domæne i oncoføtalt fibronectin (ED-B).
5. Bindingsprotein ifølge et hvilket som helst af kravene 1 til 4 omfattende aminosyresekvensen SEQ ID NO: 3.
6. Fusionsprotein omfattende et bindingsprotein ifølge et hvilket som helst af kravene 1 til 5 fusioneret til en farmaceutisk og/eller diagnostisk aktiv komponent.
7. Fusionsprotein ifølge krav 6, hvori komponenten er valgt fra gruppen bestående af: (i) cytokiner, fortrinsvis cytokiner valgt fra gruppen bestående af IL-2, IL-12, TNF-α, IFN-a, IFN-β, IFN-γ, IL-10, IL-15, IL-24, GM-CSF, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-11, IL-13, LIF, CD80, B70,TNF-3, LT-β, CD-40-ligand, Fas-ligand, TGF-β, IL-1a og IL-Ιβ; (ii) toksiske forbindelser, fortrinsvis små organiske forbindelser eller polypeptider, fortrinsvis toksiske forbindelser valgt fra gruppen bestående calicheamicin, neocarzinostatin, esperamicin, dynemicin, kedarcidin, maduropeptin, doxorubicin, daunorubicin, auristatin, Ricin-A-kæde, modeccin, trunkeret Pseudomonas-exotoxin-A, diphtheritistoxin og rekombinant gelonin; (iii) chemokiner, fortrinsvis chemokiner valgt fra gruppen bestående af IL-8, GRO-a, GRO-β, GRO-y, ENA-78, LDGF-PBP, GCP-2, PF4, Mig, IP-10, SDF-Ια/β, BUNZO/STRC33, l-TAC, BLC/BCA-1, ΜΙΡ-1α, ΜΙΡ-1β, MDC, TECK, TARC, RANTES, HCC-1, HCC-4, DC-CK1, ΜΙΡ-3α, ΜΙΡ-3β, MCP-1-5, Eotaxin, Eotaxin-2, I-309, MPIF-1, 6Ckine, CTACK, MEC, Lymphotactin og Fractalkin; (iv) fluorescerende farvestoffer, fortrinsvis en komponent valgt blandt Alexa-fluor-eller Cy-farvestoffer; (v) fotosensibilisatorer, fortrinsvis bis(triethanolamin)Sn(IV)-chlorin-6 (SnChee); (vi) pro-coagulant-faktorer, fortrinsvis vævsfaktorer; (vii) enzymer til aktivering af promedikamenter, fortrinsvis enzymer valgt fra gruppen bestående af carboxypeptidaser, glucuronidaser og glucosidaser; (vii) radionuclider, enten fra gruppen af γ-emitterende isotoper, fortrinsvis 99mTc, 123l, 111 In, eller fra gruppen af positron-emittere, fortrinsvis 18F, 64Cu, 68Ga, 86Y, 124l, eller fra gruppen af β-emittere, fortrinsvis 1311,90Y, 177Lu, 67Cu, eller fra gruppen af a-emittere, fortrinsvis 213Bi, 211At; og (viii) funktionelle Fc-domæner, fortrinsvis humane funktionelle Fc-domæner.
8. Fusionsprotein ifølge krav 6 eller 7, som endvidere omfatter en komponent, der modulerer serum-halveringstiden, fortrinsvis en komponent valgt fra gruppen bestående af polyethylenglycol (PEG), immunoglobulin og albuminbindende peptider.
9. Fusionsprotein ifølge et hvilket som helst af kravene 6 til 8 omfattende bindingsproteinet ifølge krav 5 eller 6.
10. Polynukleotid, der koder for et bindingsprotein eller et fusionsprotein ifølge et hvilket som helst af kravene 1 til 9.
11. Vektor, der omfatter et polynukleotid ifølge krav 10.
12. Værtscelle, der omfatter et polynukleotid ifølge krav 10 og/eller en vektor ifølge krav 11.
13. Anvendelse af et bindings- eller fusionsprotein ifølge et hvilket som helst af kravene 1 til 9 til fremstilling af et medikament eller et diagnostisk middel, fortrinsvis et medikament til behandling af cancer eller et diagnostisk middel til diagnose af cancer.
14. Farmaceutisk eller diagnostisk sammensætning omfattende et bindings- eller fusionsprotein ifølge et hvilket som helst af kravene 1 til 9 og eventuelt et farmaceutisk acceptabelt excipiens.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06017336A EP1892248A1 (en) | 2006-08-21 | 2006-08-21 | Specific and high affinity binding proteins comprising modified SH3 domains of FYN kinase |
| PCT/EP2007/007324 WO2008022759A2 (en) | 2006-08-21 | 2007-08-20 | Specific and high affinity binding proteins comprising modified sh3 domains of fyn kinase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2054432T3 true DK2054432T3 (da) | 2015-09-14 |
Family
ID=37074627
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK07801764.7T DK2054432T3 (da) | 2006-08-21 | 2007-08-20 | Specifikke proteiner med høj bindingsaffinitet med modificerede SH3-domæner i FYN-kinase |
Country Status (19)
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|---|---|
| US (1) | US20100119446A1 (da) |
| EP (2) | EP1892248A1 (da) |
| JP (2) | JP2010500875A (da) |
| KR (1) | KR101159903B1 (da) |
| CN (2) | CN103087172A (da) |
| AU (1) | AU2007287807B2 (da) |
| BR (1) | BRPI0715818B8 (da) |
| CA (1) | CA2661160C (da) |
| CY (1) | CY1116952T1 (da) |
| DK (1) | DK2054432T3 (da) |
| ES (1) | ES2548438T3 (da) |
| HU (1) | HUE027941T2 (da) |
| MX (1) | MX2009001867A (da) |
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| PL (1) | PL2054432T3 (da) |
| PT (1) | PT2054432E (da) |
| RU (1) | RU2478707C2 (da) |
| SI (1) | SI2054432T1 (da) |
| WO (1) | WO2008022759A2 (da) |
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| BRPI0715818A2 (pt) | 2013-07-23 |
| BRPI0715818B1 (pt) | 2020-04-22 |
| RU2478707C2 (ru) | 2013-04-10 |
| SI2054432T1 (sl) | 2015-10-30 |
| PL2054432T3 (pl) | 2015-11-30 |
| WO2008022759A3 (en) | 2008-04-17 |
| BRPI0715818B8 (pt) | 2021-05-25 |
| CY1116952T1 (el) | 2017-04-05 |
| PT2054432E (pt) | 2015-10-15 |
| EP2054432B1 (en) | 2015-07-15 |
| US20100119446A1 (en) | 2010-05-13 |
| CA2661160C (en) | 2016-11-08 |
| CN101506230A (zh) | 2009-08-12 |
| MX2009001867A (es) | 2009-03-03 |
| EP2054432A2 (en) | 2009-05-06 |
| ES2548438T3 (es) | 2015-10-16 |
| EP1892248A1 (en) | 2008-02-27 |
| KR101159903B1 (ko) | 2012-06-26 |
| JP2013078316A (ja) | 2013-05-02 |
| AU2007287807B2 (en) | 2011-10-13 |
| JP5726837B2 (ja) | 2015-06-03 |
| CA2661160A1 (en) | 2008-02-28 |
| HUE027941T2 (en) | 2016-11-28 |
| RU2009110180A (ru) | 2010-09-27 |
| KR20090053926A (ko) | 2009-05-28 |
| NZ574889A (en) | 2011-11-25 |
| WO2008022759A2 (en) | 2008-02-28 |
| JP2010500875A (ja) | 2010-01-14 |
| AU2007287807A1 (en) | 2008-02-28 |
| CN103087172A (zh) | 2013-05-08 |
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