DK2031366T3 - Automatiseret proteinanalysator - Google Patents

Automatiseret proteinanalysator Download PDF

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Publication number
DK2031366T3
DK2031366T3 DK08163213.5T DK08163213T DK2031366T3 DK 2031366 T3 DK2031366 T3 DK 2031366T3 DK 08163213 T DK08163213 T DK 08163213T DK 2031366 T3 DK2031366 T3 DK 2031366T3
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DK
Denmark
Prior art keywords
protein
dye
colorimeter
sample
reaction vessel
Prior art date
Application number
DK08163213.5T
Other languages
English (en)
Inventor
Timothy A Zawatsky
David L Herman
Michael J Collins Sr
Joseph L Lambert
Original Assignee
Cem Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cem Corp filed Critical Cem Corp
Application granted granted Critical
Publication of DK2031366T3 publication Critical patent/DK2031366T3/da

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G01N33/12—Meat; fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/105831—Protein or peptide standard or control [e.g., hemoglobin, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Claims (5)

1. Automatiseret proteinanalysator omfattende: en homogenisator (11) til at reducere proteinprøver til små partikler; en reaktionsbeholder (14) i materialeoverføringskontakt med homogenisatoren; et reservoir (16) til bindingsfarvesammensætning i fluid kontakt med reaktionsbeholderen (14); en målepumpe (20) i fluid kontakt med reservoiret (16) og reaktionsbeholderen (14) til fordeling af diskrete forudbestemte mængder afen bindingsfarvesammensætning til reaktionsbeholderen (14); et filter (21) i fluid kontakt med reaktionsbeholderen (14) til at adskille faste stoffer fra filtrat efter en farvebindingsreaktion har fundet sted i reaktionskedlen; et kolorimeter (22) i fluid kontakt med filteret (21) og reaktionsbeholderen (14) til måling af absorbansen af filtratet fra reaktionsbeholderen (14) og filteret (21); og en pumpe (25), opstrøms for kolorimeteret (22) i forhold til reaktionsbeholderen (14), til at overføre filtrat fra reaktionsbeholderen (14) til kolorimeteret (22), hvor den automatiserede proteinanalysator yderligere omfatter: en ventil (23) mellem reservoiret (16) og reaktionsbeholderen (14); og en fluidlinje (24) mellem ventilen (23) og til kolorimeteret (22); så at bindingsfarveopløsning fra reservoiret (16) kan ledes til reaktionsbeholderen (14) eller til kolorimeteret (22) baseret på positionen af ventilen (23) til automatisk at tage referencemålinger; og en processor (31) i signalkontakt med kolorimeteret (21); og hukommelse (33) i signalkontakt med processoren (31) til lagring af absorbansresultaterne fra referencetests lavet affarve ledt direkte fra reservoiret (16) til kolorimeteret (22) og fra prøvetests lavet af farvefiltrat tilbageværende efter en reaktion med en proteinprøve, til sammenligning af absorbansen af reference- og prøvetest, og til beregning af proteindholdet afen prøve baseret på sammenligningerne.
2. Proteinanalysator ifølge krav 1, hvor homogenisatoren (11) er valgt fra gruppen bestående af blandere, møller, og kombinationer deraf.
3. Proteinanalysator ifølge kravene 1 eller 2 yderligere omfattende et display (34) i signalkontakt med processoren (31) til visning af dele valgt fra gruppen bestående af absorbans- og proteinindhold.
4. Proteinanalysator ifølge kravene 1 til 3 yderligere omfattende et vaskereservoir (35) i kontakt med homogenisatoren (11) til at forsyne homogenisatoren (11) med et vaskefluid.
5. Proteinanalysator ifølge kravene 1 til 4 yderligere omfattende et dræn (37) fra homogenisatoren (11) til at bære affaldsvaskefluid fra homogenisatoren (11).
DK08163213.5T 2007-08-29 2008-08-28 Automatiseret proteinanalysator DK2031366T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US11/846,598 US8147759B2 (en) 2007-08-29 2007-08-29 Automated protein analyzer

Publications (1)

Publication Number Publication Date
DK2031366T3 true DK2031366T3 (da) 2018-09-17

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Family Applications (4)

Application Number Title Priority Date Filing Date
DK08163213.5T DK2031366T3 (da) 2007-08-29 2008-08-28 Automatiseret proteinanalysator
DK08163211T DK2031399T3 (da) 2007-08-29 2008-08-28 Automatiseret protein-analysefremgangsmåde
DK08171715T DK2053404T3 (da) 2007-08-29 2008-08-28 Automatiseret proteinanalysekit
DK08163212T DK2031400T3 (da) 2007-08-29 2008-08-28 Automatisk proteinanalysefremgangsmåde

Family Applications After (3)

Application Number Title Priority Date Filing Date
DK08163211T DK2031399T3 (da) 2007-08-29 2008-08-28 Automatiseret protein-analysefremgangsmåde
DK08171715T DK2053404T3 (da) 2007-08-29 2008-08-28 Automatiseret proteinanalysekit
DK08163212T DK2031400T3 (da) 2007-08-29 2008-08-28 Automatisk proteinanalysefremgangsmåde

Country Status (4)

Country Link
US (5) US8147759B2 (da)
EP (5) EP2031399B1 (da)
AT (1) ATE514949T1 (da)
DK (4) DK2031366T3 (da)

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CN104165886A (zh) * 2014-08-01 2014-11-26 湖北富邦科技股份有限公司 一种谷物蛋白质的测定方法
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Also Published As

Publication number Publication date
DK2031399T3 (da) 2013-07-08
DK2053404T3 (da) 2013-04-22
EP2053404B1 (en) 2013-03-06
DK2031400T3 (da) 2011-08-22
US20100291686A1 (en) 2010-11-18
US7968344B2 (en) 2011-06-28
EP3435086A2 (en) 2019-01-30
US20150301061A1 (en) 2015-10-22
EP2031399B1 (en) 2013-06-05
EP2031399A1 (en) 2009-03-04
US8147759B2 (en) 2012-04-03
EP2031400A1 (en) 2009-03-04
EP2031366A3 (en) 2010-02-24
EP2031400B1 (en) 2011-06-29
US20090061522A1 (en) 2009-03-05
EP3435086A3 (en) 2019-05-08
US20140024120A1 (en) 2014-01-23
EP2031366A2 (en) 2009-03-04
EP2053404A1 (en) 2009-04-29
US20100290949A1 (en) 2010-11-18
US9091632B2 (en) 2015-07-28
US8663993B2 (en) 2014-03-04
ATE514949T1 (de) 2011-07-15
EP2031366B1 (en) 2018-07-11

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