DK1835022T3 - Celledyrkningsfremgangsmåde og udnyttelse af denne - Google Patents
Celledyrkningsfremgangsmåde og udnyttelse af denne Download PDFInfo
- Publication number
- DK1835022T3 DK1835022T3 DK05819639.5T DK05819639T DK1835022T3 DK 1835022 T3 DK1835022 T3 DK 1835022T3 DK 05819639 T DK05819639 T DK 05819639T DK 1835022 T3 DK1835022 T3 DK 1835022T3
- Authority
- DK
- Denmark
- Prior art keywords
- cell
- medium
- culture
- protein
- antibody
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0031—Serum-free culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/80—Undefined extracts from animals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (14)
1. Fremgangsmåde til dyrkning afen dyrecelle med undtagelse afen IgM-producerende celle omfattende starte dyrkning i et opstartsmedium indeholdende et enzymatisk nedbrydningsprodukt af fiskekød tilsat dertil og tilføre mindst én gang et fødemedium indeholdende et enzymatisk nedbrydningsprodukt af fiskekød til mediet under dyrkning.
2. Fremgangsmåden ifølge krav 1, hvor cellen dyrkes ved fed-batch-dyrkning.
3. Fremgangsmåden ifølge krav 1 eller 2, hvor cellen er en celle ind i hvilken et gen der koder for et protein af interesse er blevet overført.
4. Fremgangsmåden ifølge krav 3, hvor proteinet af interesse er et antistof.
5. Fremgangsmåden ifølge et hvilket som helst af kravene 2 til 4, hvor cellen er en pattedyrcelle.
6. Fremgangsmåden ifølge krav 5, hvor pattedyrcellen er en CHO-celle.
7. Fremgangsmåde til at producere et protein ved dyrkning af en dyrecelle med undtagelse af en IgM-producerende celle omfattende at starte dyrkning i et opstartsmedium indeholdende en enzymatisk nedbrydningsprodukt af fiskekød tilsat dertil og tilføre mindst én gang et fødemedium indeholdende et enzymatisk nedbrydningsprodukt af fiskekød til mediet under dyrkning.
8. Fremgangsmåden ifølge krav 7, hvor cellen dyrkes ved fed-batch-dyrkning.
9. Fremgangsmåden ifølge krav 7 eller 8, hvor cellen er en celle ind i hvilken et gen der koder for et protein af interesse er blevet overført.
10. Fremgangsmåden ifølge krav 9, hvor proteinet af interesse er et antistof.
11. Fremgangsmåden ifølge et hvilket som helst af kravene 7 til 10, hvor cellen er en pattedyrcelle.
12. Fremgangsmåden ifølge krav 11, hvor pattedyrcellen er en CHO-celle.
13. Fremgangsmåden ifølge krav 2 eller 8, hvor koncentrationen af det tilsatte enzymatiske nedbrydningsprodukt af fiskekød i opstartsmediet er 3 til 20 g/l og i fødemediet 5 til 150 g/l.
14. Fremgangsmåden ifølge krav 4 eller 10, hvor antistoffet er et IgG-antistof.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2005000747 | 2005-01-05 | ||
| PCT/JP2005/023709 WO2006073070A1 (ja) | 2005-01-05 | 2005-12-26 | 細胞の培養方法およびその利用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1835022T3 true DK1835022T3 (da) | 2015-02-23 |
Family
ID=36647552
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK05819639.5T DK1835022T3 (da) | 2005-01-05 | 2005-12-26 | Celledyrkningsfremgangsmåde og udnyttelse af denne |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US9714410B2 (da) |
| EP (2) | EP1835022B1 (da) |
| JP (1) | JP4818936B2 (da) |
| KR (2) | KR20130101161A (da) |
| CN (2) | CN104651295A (da) |
| AU (1) | AU2005323643B2 (da) |
| CA (1) | CA2593521C (da) |
| DK (1) | DK1835022T3 (da) |
| ES (1) | ES2535230T3 (da) |
| HK (1) | HK1207883A1 (da) |
| IL (1) | IL184262A (da) |
| MX (1) | MX2007007842A (da) |
| PL (1) | PL1835022T3 (da) |
| SI (1) | SI1835022T1 (da) |
| TW (1) | TWI409332B (da) |
| WO (1) | WO2006073070A1 (da) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2382488T3 (es) | 1997-03-21 | 2012-06-08 | Chugai Seiyaku Kabushiki Kaisha | Un agente preventivo o terapéutico para enfermedades mediadas por células t sensibilizadas que comprende un antagonista de il-6 como ingrediente activo |
| UA80091C2 (en) | 2001-04-02 | 2007-08-27 | Chugai Pharmaceutical Co Ltd | Remedies for infant chronic arthritis-relating diseases and still's disease which contain an interleukin-6 (il-6) antagonist |
| US20050118163A1 (en) * | 2002-02-14 | 2005-06-02 | Hidefumi Mizushima | Antibody-containing solution pharmaceuticals |
| GB2401040A (en) * | 2003-04-28 | 2004-11-03 | Chugai Pharmaceutical Co Ltd | Method for treating interleukin-6 related diseases |
| EP3736295A1 (en) * | 2004-03-24 | 2020-11-11 | Chugai Seiyaku Kabushiki Kaisha | Subtypes of humanized antibody against interleukin-6 receptor |
| KR101685246B1 (ko) * | 2006-07-14 | 2016-12-09 | 디피엑스 홀딩스 비.브이. | 개선된 세포 배양 방법 |
| PE20091174A1 (es) | 2007-12-27 | 2009-08-03 | Chugai Pharmaceutical Co Ltd | Formulacion liquida con contenido de alta concentracion de anticuerpo |
| DK2130906T3 (da) * | 2008-06-04 | 2014-05-12 | Biosilta Oy | Fremgangsmåde til tilførsel af vækstkomponenter til cellekulturer |
| PL2493922T3 (pl) | 2009-10-26 | 2017-07-31 | F.Hoffmann-La Roche Ag | Sposób wytwarzania glikozylowanych immunoglobulin |
| EP4029881A1 (en) | 2010-11-08 | 2022-07-20 | F. Hoffmann-La Roche AG | Subcutaneously administered anti-il-6 receptor antibody |
| BR112015032960B1 (pt) | 2013-07-04 | 2021-01-05 | F. Hoffmann-La Roche Ag | imunoensaio suprimido por interferência para detectar anticorpos anti-fármaco em amostras de soro |
| CN107208025A (zh) | 2014-11-25 | 2017-09-26 | 康宁股份有限公司 | 延续细胞培养基的材料和方法 |
| AR104050A1 (es) * | 2015-03-26 | 2017-06-21 | Chugai Pharmaceutical Co Ltd | Proceso de producción con iones de cobre controlados |
| US11484591B2 (en) | 2016-02-22 | 2022-11-01 | Ohio State Innovation Foundation | Chemoprevention using controlled-release formulations of anti-interleukin 6 agents, synthetic vitamin A analogues or metabolites, and estradiol metabolites |
| US11033496B2 (en) | 2017-03-17 | 2021-06-15 | The Regents Of The University Of Michigan | Nanoparticles for delivery of chemopreventive agents |
| US20220177978A1 (en) | 2019-04-02 | 2022-06-09 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods of predicting and preventing cancer in patients having premalignant lesions |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU632065B2 (en) | 1988-09-23 | 1992-12-17 | Novartis Vaccines And Diagnostics, Inc. | Cell culture medium for enhanced cell growth, culture longevity and product expression |
| AU668349B2 (en) | 1991-04-25 | 1996-05-02 | Chugai Seiyaku Kabushiki Kaisha | Reconstituted human antibody against human interleukin 6 receptor |
| US5426045A (en) | 1993-12-16 | 1995-06-20 | Sea Run Holdings, Inc. | Method for culturing mammalian cells in a medium containing fish serum |
| WO1995023212A1 (en) * | 1994-02-23 | 1995-08-31 | Sea Run Holdings, Inc. | Method for culturing mammalian cells and insect cells in a medium containing fish serum |
| US5498540A (en) | 1994-02-23 | 1996-03-12 | Sea Run Holdings, Inc. | Method for culturing insect cells in a medium containing fish serum |
| US5401653A (en) | 1994-02-23 | 1995-03-28 | Sea Run Holdings, Inc. | Method for culturing insect cells in a medium containing fish serum |
| US5443894A (en) | 1994-07-29 | 1995-08-22 | Ucar Carbon Technology Corporation | Fire retardant oriented strand board structure element |
| JP3630453B2 (ja) | 1994-09-30 | 2005-03-16 | 中外製薬株式会社 | Ｉｌ−６レセプター抗体を有効成分とする未熟型骨髄腫細胞治療剤 |
| US6537782B1 (en) | 1998-06-01 | 2003-03-25 | Chugai Seiyaku Kabushiki Kaisha | Media for culturing animal cells and process for producing protein by using the same |
| CA2417689C (en) | 2002-03-05 | 2006-05-09 | F. Hoffmann-La Roche Ag | Improved methods for growing mammalian cells in vitro |
| JP3822137B2 (ja) * | 2002-05-20 | 2006-09-13 | 中外製薬株式会社 | 動物細胞培養用培地の添加剤およびそれを用いたタンパク質の製造方法 |
| JP4865539B2 (ja) * | 2003-05-09 | 2012-02-01 | クルセル ホランド ベー ヴェー | Ｅ１不死化細胞の培養物及び該培養物を培養して該培養物から得られる産物の収量を増加させる方法 |
| JP4353731B2 (ja) | 2003-06-10 | 2009-10-28 | 前澤工業株式会社 | 溶液中のヒ素の除去方法 |
| TW200510532A (en) | 2003-07-15 | 2005-03-16 | Chugai Pharmaceutical Co Ltd | IgM production by transformed cell and method of quantifying the same |
| TW200530269A (en) | 2003-12-12 | 2005-09-16 | Chugai Pharmaceutical Co Ltd | Anti-Mpl antibodies |
-
2005
- 2005-12-26 AU AU2005323643A patent/AU2005323643B2/en active Active
- 2005-12-26 KR KR1020137022551A patent/KR20130101161A/ko not_active Application Discontinuation
- 2005-12-26 WO PCT/JP2005/023709 patent/WO2006073070A1/ja active Application Filing
- 2005-12-26 CA CA2593521A patent/CA2593521C/en active Active
- 2005-12-26 EP EP05819639.5A patent/EP1835022B1/en active Active
- 2005-12-26 PL PL05819639T patent/PL1835022T3/pl unknown
- 2005-12-26 SI SI200531955T patent/SI1835022T1/sl unknown
- 2005-12-26 MX MX2007007842A patent/MX2007007842A/es active IP Right Grant
- 2005-12-26 CN CN201510048283.1A patent/CN104651295A/zh active Pending
- 2005-12-26 ES ES05819639.5T patent/ES2535230T3/es active Active
- 2005-12-26 EP EP14189055.8A patent/EP2843040A1/en not_active Withdrawn
- 2005-12-26 KR KR1020077015339A patent/KR101573529B1/ko active IP Right Grant
- 2005-12-26 JP JP2006550737A patent/JP4818936B2/ja active Active
- 2005-12-26 DK DK05819639.5T patent/DK1835022T3/da active
- 2005-12-26 CN CNA2005800395433A patent/CN101061215A/zh active Pending
- 2005-12-26 US US11/793,784 patent/US9714410B2/en active Active
-
2006
- 2006-01-02 TW TW095100075A patent/TWI409332B/zh active
-
2007
- 2007-06-27 IL IL184262A patent/IL184262A/en active IP Right Grant
-
2015
- 2015-08-31 HK HK15108493.8A patent/HK1207883A1/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| KR20130101161A (ko) | 2013-09-12 |
| IL184262A (en) | 2015-09-24 |
| JPWO2006073070A1 (ja) | 2008-06-12 |
| SI1835022T1 (sl) | 2015-04-30 |
| TW200636068A (en) | 2006-10-16 |
| EP1835022A1 (en) | 2007-09-19 |
| AU2005323643B2 (en) | 2011-07-21 |
| TWI409332B (zh) | 2013-09-21 |
| AU2005323643A1 (en) | 2006-07-13 |
| CN104651295A (zh) | 2015-05-27 |
| MX2007007842A (es) | 2007-08-21 |
| KR101573529B1 (ko) | 2015-12-01 |
| CA2593521A1 (en) | 2006-07-13 |
| CN101061215A (zh) | 2007-10-24 |
| US9714410B2 (en) | 2017-07-25 |
| WO2006073070A1 (ja) | 2006-07-13 |
| EP2843040A1 (en) | 2015-03-04 |
| CA2593521C (en) | 2016-06-21 |
| ES2535230T3 (es) | 2015-05-06 |
| KR20070090998A (ko) | 2007-09-06 |
| IL184262A0 (en) | 2007-10-31 |
| PL1835022T3 (pl) | 2015-06-30 |
| JP4818936B2 (ja) | 2011-11-16 |
| US20080124761A1 (en) | 2008-05-29 |
| HK1207883A1 (en) | 2016-02-12 |
| EP1835022B1 (en) | 2015-02-11 |
| EP1835022A4 (en) | 2009-11-11 |
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