DK181386D0 - Proteaseresistente derivater af enkelt-kaede-urokinase, fremgangsmaade til fremstilling deraf, nucleinsyrer, replicerbare vektorer og vaertsceller til anvendelse ved fremgangsmaaden og farmaceutiske praeparater af urokinase-derivaterne - Google Patents

Proteaseresistente derivater af enkelt-kaede-urokinase, fremgangsmaade til fremstilling deraf, nucleinsyrer, replicerbare vektorer og vaertsceller til anvendelse ved fremgangsmaaden og farmaceutiske praeparater af urokinase-derivaterne

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Publication number
DK181386D0
DK181386D0 DK181386A DK181386A DK181386D0 DK 181386 D0 DK181386 D0 DK 181386D0 DK 181386 A DK181386 A DK 181386A DK 181386 A DK181386 A DK 181386A DK 181386 D0 DK181386 D0 DK 181386D0
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Prior art keywords
urokinase
pharmaceutical
sites
chain
variant
Prior art date
Application number
DK181386A
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English (en)
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DK175304B1 (da
DK181386A (da
Inventor
Michael Blaber
Herbert Louis Heyneker
Gordon Allen Vehar
Original Assignee
Genentech Inc
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Publication of DK181386D0 publication Critical patent/DK181386D0/da
Publication of DK181386A publication Critical patent/DK181386A/da
Application granted granted Critical
Publication of DK175304B1 publication Critical patent/DK175304B1/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK198601813A 1985-04-22 1986-04-21 Præparat der omfatter enkelt-kæde-urokinase, nukleinsyre der koder for enkelt-kæde-urokinase, replicerbar vektor der omfatter en sådan nukleinsyre, rekombinant værtscelle der er transformeret med en sådan vektor, fremgangsmåde til fremstilling..... DK175304B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US72546885A 1985-04-22 1985-04-22
US72546885 1985-04-22
US76685885 1985-08-16
US06/766,858 US5219569A (en) 1985-04-22 1985-08-16 Protease resistant urokinase

Publications (3)

Publication Number Publication Date
DK181386D0 true DK181386D0 (da) 1986-04-21
DK181386A DK181386A (da) 1986-10-23
DK175304B1 DK175304B1 (da) 2004-08-16

Family

ID=27111148

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198601813A DK175304B1 (da) 1985-04-22 1986-04-21 Præparat der omfatter enkelt-kæde-urokinase, nukleinsyre der koder for enkelt-kæde-urokinase, replicerbar vektor der omfatter en sådan nukleinsyre, rekombinant værtscelle der er transformeret med en sådan vektor, fremgangsmåde til fremstilling.....

Country Status (10)

Country Link
US (1) US5219569A (da)
EP (1) EP0200451B1 (da)
AT (1) ATE98686T1 (da)
DD (2) DD264939A5 (da)
DE (1) DE3689386T2 (da)
DK (1) DK175304B1 (da)
ES (1) ES8800347A1 (da)
GR (1) GR861036B (da)
HU (1) HU202586B (da)
PT (1) PT82428B (da)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5204255A (en) * 1986-01-31 1993-04-20 Sagami Chemical Research Center Hybrid tissue plasminogen activator/urokinase polypeptides
EP0236040A3 (en) * 1986-02-26 1989-05-03 Collaborative Research Inc. Amino acid modified prourokinase and method of preparation
JPH0761266B2 (ja) * 1986-07-03 1995-07-05 株式会社ミドリ十字 変異ヒトプロウロキナ−ゼ
WO1988005081A2 (en) * 1986-12-30 1988-07-14 Cetus Corporation Novel plasminogen activator
US5009888A (en) * 1987-04-13 1991-04-23 Genzyme Corporation Therapeutic enzyme-antibody complexes
MY103358A (en) * 1987-04-15 1993-06-30 Novartis Ag Process for the production of protiens.
WO1989001513A1 (en) * 1987-08-19 1989-02-23 Sagami Chemical Research Center Fast-acting prourokinase
DK450187D0 (da) * 1987-08-28 1987-08-28 Novo Industri As Fremgangsmaade til fremstilling af proteiner
US5591603A (en) * 1987-08-28 1997-01-07 Novo Nordisk A/S Process for preparing aprotinin and aprotinin analogs in yeast cells
DE3735917A1 (de) * 1987-10-23 1989-05-03 Basf Ag Neue polypeptide, ihre herstellung und verwendung
DE3735916A1 (de) * 1987-10-23 1989-05-03 Basf Ag Neue polypeptide, ihre herstellung und verwendung
JPH0322979A (ja) * 1989-06-19 1991-01-31 Kyowa Hakko Kogyo Co Ltd 新規プラスミノーゲン活性化因子
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
GB8915658D0 (en) * 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
US5240845A (en) * 1989-07-11 1993-08-31 Otsuka Pharmaceutical Factory, Ltd. Mutated streptokinase proteins
US5087572A (en) * 1989-12-01 1992-02-11 Genentech, Inc. Dna encoding human plasminogen modified at the cleavage site
CA2162855A1 (en) 1993-06-01 1994-12-08 Steven Rosenberg Expression of urokinase plasminogen activator inhibitors
US6248715B1 (en) 1993-06-01 2001-06-19 Chiron Corporation Method of treating a urokinase-type plasminogen activator-mediated disorder
DE69528614T2 (de) * 1994-07-07 2003-06-18 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno, Delft Modifizierte Proenzyme als Substrate für proteolische Enzyme
WO1997033984A1 (en) * 1996-03-12 1997-09-18 Novo Nordisk A/S Novel achromobacter lyticus protease variants
CN1311076C (zh) * 2003-04-16 2007-04-18 普罗特奥姆技术公司 生产重组尿激酶的方法
US7361493B1 (en) 2004-05-26 2008-04-22 The United States Of America As Represented By The Secretary Of The Department Of Veterans Affairs Production of urokinase in a three-dimensional cell culture
GB2446550B (en) * 2005-11-28 2010-12-08 Proteomtech Inc Methods for production of recombinant alpha1-antitrypsin
US20160146834A1 (en) 2013-07-12 2016-05-26 Emory University Diagnostic assay to predict cardiovascular risk
US9783587B1 (en) 2014-05-09 2017-10-10 Florida State University Research Foundation Synthetic foldable proteins generated from peptide segments of folding nuclei of reference proteins

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4381346A (en) * 1979-11-13 1983-04-26 Huasin Syed S Isolation of plasminogen activators useful as therapeutic and diagnostic agents
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
IL68366A (en) * 1982-04-15 1990-08-31 Genentech Inc Process for expressing a gene encoding a protein comprising the enzymatic portion of human urokinase in a microorganism or cell culture
AU5390286A (en) * 1985-01-25 1986-08-13 Sagami Chemical Research Center Stabilized human prourokinase
EP0236040A3 (en) * 1986-02-26 1989-05-03 Collaborative Research Inc. Amino acid modified prourokinase and method of preparation

Also Published As

Publication number Publication date
ES554249A0 (es) 1987-11-16
DE3689386T2 (de) 1994-05-19
HU202586B (en) 1991-03-28
DK175304B1 (da) 2004-08-16
DD264939A5 (de) 1989-02-15
GR861036B (en) 1986-07-16
DK181386A (da) 1986-10-23
PT82428A (en) 1986-05-01
ATE98686T1 (de) 1994-01-15
US5219569A (en) 1993-06-15
EP0200451B1 (en) 1993-12-15
PT82428B (pt) 1988-03-03
ES8800347A1 (es) 1987-11-16
DD251791A5 (de) 1987-11-25
DE3689386D1 (de) 1994-01-27
HUT40696A (en) 1987-01-28
EP0200451A1 (en) 1986-11-05

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