DK181286A - Mutanter af human vaevplasminogenaktivator (t-pa), dna-sekvenser, som koder for disse, udtrykkelsesvektorer, som kan udtrykke dna-sekvenserne, mikroorganismer og cellekulturer transformeret med vektorerne og farmaceutiske praeparater af t-pa-mutanterne - Google Patents

Mutanter af human vaevplasminogenaktivator (t-pa), dna-sekvenser, som koder for disse, udtrykkelsesvektorer, som kan udtrykke dna-sekvenserne, mikroorganismer og cellekulturer transformeret med vektorerne og farmaceutiske praeparater af t-pa-mutanterne Download PDF

Info

Publication number
DK181286A
DK181286A DK181286A DK181286A DK181286A DK 181286 A DK181286 A DK 181286A DK 181286 A DK181286 A DK 181286A DK 181286 A DK181286 A DK 181286A DK 181286 A DK181286 A DK 181286A
Authority
DK
Denmark
Prior art keywords
dna sequences
expression vectors
microorganisms
express
plasminogen activator
Prior art date
Application number
DK181286A
Other languages
English (en)
Other versions
DK175380B1 (da
DK181286D0 (da
Inventor
Herbert Louis Heyneker
Gordon Allen Vehar
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of DK181286D0 publication Critical patent/DK181286D0/da
Publication of DK181286A publication Critical patent/DK181286A/da
Application granted granted Critical
Publication of DK175380B1 publication Critical patent/DK175380B1/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Description

Figure DK181286AD00031
DK198601812A 1985-04-22 1986-04-21 Mutant af human vævsplasminogenaktivator, DNA-sekvens som koder herfor, replicerbar udtrykkelsesvektor til at udtrykke denne DNA-sekvens, mikroorganisme transformeret med denne vektor, cellekulturer transformeret med denne.... DK175380B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US72546885A 1985-04-22 1985-04-22
US72546885 1985-04-22
US84669786A 1986-04-01 1986-04-01
US84669786 1986-04-01

Publications (3)

Publication Number Publication Date
DK181286D0 DK181286D0 (da) 1986-04-21
DK181286A true DK181286A (da) 1986-10-23
DK175380B1 DK175380B1 (da) 2004-09-20

Family

ID=27111150

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198601812A DK175380B1 (da) 1985-04-22 1986-04-21 Mutant af human vævsplasminogenaktivator, DNA-sekvens som koder herfor, replicerbar udtrykkelsesvektor til at udtrykke denne DNA-sekvens, mikroorganisme transformeret med denne vektor, cellekulturer transformeret med denne....

Country Status (21)

Country Link
US (1) US5147643A (da)
EP (1) EP0199574B1 (da)
JP (2) JPH0740940B2 (da)
AT (1) ATE68825T1 (da)
AU (1) AU596356B2 (da)
CA (1) CA1341580C (da)
DD (1) DD258026A5 (da)
DE (2) DE3613390C2 (da)
DK (1) DK175380B1 (da)
ES (2) ES8802184A1 (da)
FI (1) FI98930C (da)
FR (1) FR2581652B1 (da)
GB (1) GB2173804B (da)
GR (1) GR861037B (da)
HU (1) HU206520B (da)
IE (1) IE58574B1 (da)
IL (1) IL78571A (da)
MY (1) MY102550A (da)
NO (1) NO175216C (da)
NZ (1) NZ215920A (da)
PT (1) PT82429B (da)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58149871A (ja) * 1982-02-27 1983-09-06 本田技研工業株式会社 二，三輪車の盗難防止装置
JPS6087751A (ja) * 1983-10-20 1985-05-17 House Food Ind Co Ltd レトルト豆腐の製造法
EP0201153A3 (en) * 1985-02-09 1987-10-07 Beecham Group Plc Modified enzyme and process for its preparation
HUT41632A (en) * 1985-03-06 1987-05-28 Survival Technology Process for preparing agents for the enhancement of protein absorption
US5736134A (en) * 1985-04-22 1998-04-07 Genentech, In.C Tissue plasminogen activator variants
DE3613390C2 (de) * 1985-04-22 2001-10-11 Genentech Inc Einkettige Human-Gewebeplasminogen-Aktivator (t-PA)-Mutanten, kodierende DNA, Expressionsvektor, Mikroorganismus, Zellkultur und pharmazeutische Zusammensetzung
US5756093A (en) * 1985-04-22 1998-05-26 Genentech, Inc. Tissue plasminogen activator variants
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
US5501853A (en) * 1985-12-23 1996-03-26 Chiron Corporation Peptide plasminogen activators
DK43387A (da) * 1986-01-29 1987-07-30 Beecham Group Plc Fibrinolytisk enzym
US5837518A (en) * 1986-01-31 1998-11-17 Genetics Institute, Inc. Thrombolytic proteins
US5071972A (en) * 1986-01-31 1991-12-10 Genetics Institute, Inc. DNA sequences encoding novel thrombolytic proteins
EP0293394B2 (en) * 1986-01-31 2003-10-29 Genetics Institute, LLC Novel thrombolytic proteins
US5258298A (en) * 1986-01-31 1993-11-02 Genetics Institute, Inc. Deletion and glycosylation mutant of human tissue plasminogen activator
IE81073B1 (en) * 1986-03-18 2000-01-12 Genentech Inc Modified human tissue-type plasminogen activator and its preparation
US5589361A (en) * 1986-03-18 1996-12-31 Genentech, Inc. Human tissue-type plasminogen activator variant
PT84589B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
PT84588B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
US5169763A (en) * 1986-04-08 1992-12-08 Transgene S.A., Institut Pasteur Viral vector coding glycoprotein of HIV-1
GB8701811D0 (en) * 1987-01-28 1987-03-04 Beecham Group Plc Compounds
MY103358A (en) * 1987-04-15 1993-06-30 Novartis Ag Process for the production of protiens.
DK275788A (da) * 1987-05-22 1989-02-03 Zymogenetics Inc Fibrinolytiske proteiner
US5200340A (en) * 1987-05-22 1993-04-06 Zymogenetics, Inc. Thrombin-activated tissue plasminogen activators
NO179874C (no) * 1987-06-04 1997-01-02 Zymogenetics Inc Fremgangsmåte for fremstilling av en t-PA-analog, DNA-sekvens som koder for t-PA-analogen, samt ekspresjonsvektor omfattende DNA-sekvensen og vertcelle omfattende denne
DK302288A (da) * 1987-06-04 1989-02-03 Zymogenetics Inc Vaevsplasminogenaktivator med modificeret kringledomaene
JPH02504102A (ja) * 1987-06-30 1990-11-29 ジェネンテク，インコーポレイテッド 血管障害治療のための改良法
US5302390A (en) * 1987-07-01 1994-04-12 Beecham Group Plc Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
IL87276A (en) * 1987-08-03 1995-07-31 Fujisawa Pharmaceutical Co Analog of a tissue plasminogen activator containing the Pringle 2 and protease regions only, DNA encoding it, processes for its preparation, and pharmaceutical preparations containing it
JP2708749B2 (ja) * 1987-08-10 1998-02-04 エーザイ株式会社 修飾型ｔＰＡ含有注射用組成物
US5244676A (en) * 1987-10-09 1993-09-14 Monsanto Company Modified tissue plasminogen activator with modified glycosylation site
US4963357A (en) * 1987-10-09 1990-10-16 Monsanto Company Tissue plasminogen activator modified by the substitution of Arg for Cys at position 73, methods and pharmaceutical compositions therefor
US5037752A (en) * 1987-10-09 1991-08-06 Monsanto Company Modified tissue plasminogen activator substituted at cysteine-73 and lysine-277
EP0312346B1 (en) * 1987-10-16 1994-08-17 Genencor International, Inc. E. coli sequence specific acid protease
DE3854195T2 (de) * 1987-10-29 1995-12-21 Yamanouchi Pharma Co Ltd Polypeptid-verbindungen.
US5504001A (en) * 1987-11-25 1996-04-02 Zymogenetics, Inc. Hybrid plasminogen activator
US5094953A (en) * 1988-03-21 1992-03-10 Genentech, Inc. Human tissue plasminogen activator variants
JPH04501204A (ja) * 1988-08-11 1992-03-05 サイオス　ノバ　インコーポレイテッド 異種タンパク発現を安定させる方法およびそれに使用するベクター
US5262170A (en) * 1988-09-02 1993-11-16 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
US5108901A (en) * 1988-09-02 1992-04-28 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5258180A (en) * 1988-09-02 1993-11-02 Genetech, Inc. Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment
US5714145A (en) * 1988-09-02 1998-02-03 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5190756A (en) * 1989-12-01 1993-03-02 Genentech, Inc. Methods and materials for expression of human plasminogen variant
US5087572A (en) * 1989-12-01 1992-02-11 Genentech, Inc. Dna encoding human plasminogen modified at the cleavage site
DE3942142A1 (de) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh Stabilisierung von glykosyliertem t-pa
NZ246432A (en) * 1991-12-16 1996-06-25 Genentech Inc Fibrin-specific t-pa with a point mutation at position 276, vector cell cultures
EP0618973B1 (en) * 1991-12-16 1996-11-27 Genentech, Inc. t-PA SUBSTITUTION VARIANTS WITH IMPROVED FIBRIN-SPECIFICITY
ES2107041T3 (es) * 1992-06-03 1997-11-16 Genentech Inc Variantes de activador de plasminogeno tisular obtenidos por glicosilacion, que presentan propiedades terapeuticas.
GB2298821A (en) * 1995-03-15 1996-09-18 Prestek Ltd A ribbon winding mechanism
DK0827751T3 (da) * 1996-09-06 2003-03-31 Chemo Sero Therapeut Res Inst Medicinsk præparat indeholdende vævsplasminogenaktivator og nicotinamid
WO1998021320A2 (en) * 1996-11-12 1998-05-22 The Scripps Research Institute TISSUE TYPE PLASMINOGEN ACTIVATOR (t-PA) VARIANTS: COMPOSITIONS AND METHODS OF USE
US6706504B1 (en) 1996-11-12 2004-03-16 The Scripps Research Institute Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
US5837688A (en) * 1996-11-27 1998-11-17 Gelfand; Mathew I. Use of thrombolytic reagents for prevention of vascular disease
AU2000268170A1 (en) * 2000-09-04 2002-03-22 Hunan Royal Biotech A cell line expressing mutant human tissue-type plasminogen activator, constructing strategy and method of expressed protein preparation
US7597895B2 (en) * 2001-02-20 2009-10-06 The Governing Council Of The University Of Toronto Signal peptides, nucleic acid molecules and methods for treatment of caries
US7556807B2 (en) 2001-02-20 2009-07-07 Kane Biotech, Inc. Signal peptides, nucleic acid molecules and methods for treatment of caries

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
ZA833174B (en) * 1982-05-05 1984-08-29 Genentech Inc Human tissue plasminogen activator
IL90047A (en) * 1982-10-19 1992-12-01 Cetus Oncology Corp Cysteine-depleted biologically active muteins other than interferon - '
US4753879A (en) * 1984-08-27 1988-06-28 Biogen N.V. Modified tissue plasminogen activators
EP0201153A3 (en) * 1985-02-09 1987-10-07 Beecham Group Plc Modified enzyme and process for its preparation
DE3613390C2 (de) * 1985-04-22 2001-10-11 Genentech Inc Einkettige Human-Gewebeplasminogen-Aktivator (t-PA)-Mutanten, kodierende DNA, Expressionsvektor, Mikroorganismus, Zellkultur und pharmazeutische Zusammensetzung
DK43387A (da) * 1986-01-29 1987-07-30 Beecham Group Plc Fibrinolytisk enzym
EP0293394B2 (en) * 1986-01-31 2003-10-29 Genetics Institute, LLC Novel thrombolytic proteins

Also Published As

Publication number Publication date
FI861673A (fi) 1986-10-23
IL78571A (en) 1991-06-30
HU206520B (en) 1992-11-30
GR861037B (en) 1986-07-16
IE861055L (en) 1986-10-22
MY102550A (en) 1992-07-31
ES554251A0 (es) 1988-04-16
FI98930C (fi) 1997-09-10
NO175216B (da) 1994-06-06
DE3682104D1 (de) 1991-11-28
JPS6224A (ja) 1987-01-06
PT82429B (pt) 1988-03-03
GB2173804A (en) 1986-10-22
HUT40703A (en) 1987-01-28
FR2581652B1 (fr) 1989-12-22
CA1341580C (en) 2008-09-30
JP2529816B2 (ja) 1996-09-04
FR2581652A1 (fr) 1986-11-14
GB2173804B (en) 1989-07-05
EP0199574B1 (en) 1991-10-23
DK175380B1 (da) 2004-09-20
EP0199574A3 (en) 1987-08-05
JPH06277071A (ja) 1994-10-04
AU596356B2 (en) 1990-05-03
PT82429A (en) 1986-05-01
AU5641686A (en) 1986-10-30
ES8802184A1 (es) 1988-04-16
ES557800A0 (es) 1990-03-01
EP0199574A2 (en) 1986-10-29
ATE68825T1 (de) 1991-11-15
ES9000011A1 (es) 1990-03-01
FI98930B (fi) 1997-05-30
DE3613390C2 (de) 2001-10-11
FI861673A0 (fi) 1986-04-21
JPH0740940B2 (ja) 1995-05-10
NO861559L (no) 1986-10-23
IL78571A0 (en) 1986-08-31
GB8609683D0 (en) 1986-05-29
DK181286D0 (da) 1986-04-21
IE58574B1 (en) 1993-10-06
NO175216C (no) 1994-09-14
DD258026A5 (de) 1988-07-06
US5147643A (en) 1992-09-15
NZ215920A (en) 1989-07-27
DE3613390A1 (de) 1986-10-30

Similar Documents

Publication Publication Date Title
DK181286A (da) Mutanter af human vaevplasminogenaktivator (t-pa), dna-sekvenser, som koder for disse, udtrykkelsesvektorer, som kan udtrykke dna-sekvenserne, mikroorganismer og cellekulturer transformeret med vektorerne og farmaceutiske praeparater af t-pa-mutanterne
US5330971A (en) Growth hormone fusion proteins, methods of production, and methods of treatment
ZA84375B (en) Human tpa production using vectors coding for dhfr protein
CA1341449C (en) Poly-kringle plasminogen activator
KR970000808B1 (en) Human proapolipoprotein a-1 expression
NZ207746A (en) Recombinant immunoglobulin preparation
EP0370036A1 (en) FACTOR VII / VIIa MODIFIED.
KR900702019A (ko) 효소원 또는 피브린 특이성을 갖는 조직 플라스미노겐 활성제
DE3682891D1 (de) Gewebeplasminogen-aktivator-mutant, dafuer kodierende rekombinante genetische information und verfahren zur herstellung dieser mutanten, deren verwendung und pharmazeutische zusammensetzungen.
GB2176489A (en) Polypeptide and polypeptide composition
HU906046D0 (en) Process for producing protein having urate-oxidase activity, gene coding that, vector of exprossion, micro organisms and transformed cells
Kinsella et al. Nucleotide sequence of a cDNA coding for mitochondrial fumarase from human liver
GB9215232D0 (en) Vaccines
ATE120491T1 (de) Ausgeglichenes, konstitutives, induzierbares transkriptionssystem.
EP0741189A4 (en) PROCESS FOR PRODUCING HUMAN GROWTH HORMONE
Jang et al. Characterization of an alkaline serine protease from an alkaline-resistant Pseudomonas sp.: cloning and expression of the protease gene in Escherichia coli
KR100391884B1 (en) Random codon based mutagenesis using transposon
WO1992019737A1 (en) Process for producing a monocyte chemotactic factor polypeptide and a strain for the production thereof
PT96134A (pt) Processo de obtencao de mutantes da oncostatina m

Legal Events

Date Code Title Description
PUP Patent expired