DK1700917T3 - Endoglucanase stce og cellulasepræparat indeholdende samme - Google Patents

Endoglucanase stce og cellulasepræparat indeholdende samme Download PDF

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Publication number
DK1700917T3
DK1700917T3 DK04792875.9T DK04792875T DK1700917T3 DK 1700917 T3 DK1700917 T3 DK 1700917T3 DK 04792875 T DK04792875 T DK 04792875T DK 1700917 T3 DK1700917 T3 DK 1700917T3
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Denmark
Prior art keywords
protein
ser
stce1
gly
ala
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DK04792875.9T
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Jinichiro C/O Health & Bioscience Lab Koga
Yuko C/O Health & Bioscience Lab Baba
Akitaka C/O Health & Bioscience Lab Nakane
Satoshi C/O Health & Bioscience Lab Hanamura
Tomoko C/O Health & Bioscience Lab Nishimura
Shuichi C/O Microbio Resources & Tech Lab Gomi
Hidetoshi C/O Health & Bioscience Lab Kubota
Toshiaki C/O Health & Bioscience Lab Kono
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Meiji Seika Pharma Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H11/00—Pulp or paper, comprising cellulose or lignocellulose fibres of natural origin only
    • D21H11/16—Pulp or paper, comprising cellulose or lignocellulose fibres of natural origin only modified by a particular after-treatment
    • D21H11/20—Chemically or biochemically modified fibres
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H25/00—After-treatment of paper not provided for in groups D21H17/00 - D21H23/00
    • D21H25/18—After-treatment of paper not provided for in groups D21H17/00 - D21H23/00 of old paper as in books, documents, e.g. restoring
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
    • D06M2101/02—Natural fibres, other than mineral fibres
    • D06M2101/04—Vegetal fibres
    • D06M2101/06—Vegetal fibres cellulosic
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M2200/00—Functionality of the treatment composition and/or properties imparted to the textile material
    • D06M2200/35—Abrasion, pilling or fibrillation resistance
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M2200/00—Functionality of the treatment composition and/or properties imparted to the textile material
    • D06M2200/50—Modified hand or grip properties; Softening compositions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Textile Engineering (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Animal Husbandry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
  • Paper (AREA)

Claims (23)

1. Protein udvalgt fra gruppen bestående af: (a) et protein, der omfatter aminosyresekvensen i SEQ ID NO: 3, (b) et modificeret protein, der omfatter en aminosyresekvens, hvori 1 til 30 aminosyrer er deleteret, substitueret, insereret eller tilsat i aminosyresekvensen i SEQ ID NO: 3, og som har en endoglucanaseaktivitet, og (c) et homologt protein, der omfatter en aminosyresekvens med mindst 90 % identitet med sekvensen i SEQ ID NO: 3, og som har en endoglucanaseaktivitet.
2. Polynukleotid, der koder for proteinet ifølge krav 1.
3. Polynukleotid udvalgt fra gruppen bestående af: (i) et polynukleotid, der omfatter nukleotidsekvensen bestående af nukleoti-der 64-948 i SEQ ID NO: 2, og (ii) et polynukleotid, der omfatter en nukleotidsekvens, hvori 1 til 30 nukleoti-der er deleteret, substitueret, insereret eller tilsat i nukleotidsekvensen bestående af nukleotider 64-948 i SEQ ID NO: 2, og som koder for et protein med en endoglucanaseaktivitet.
4. Ekspressionsvektor omfattende polynukleotidet ifølge krav 2 eller 3.
5. Værtscelle, der er transformeret med ekspressionsvektoren ifølge krav 4.
6. Værtscelle ifølge krav 5, hvor værten er en gær eller en filamentøs svamp.
7. Værtscelle ifølge krav 6, hvor gæren er en mikroorganisme, der tilhører slægten Saccharomyces, Hansenula eller Pichia.
8. Værtscelle ifølge krav 6, hvor den filamentøse svamp er en mikroorganisme, der tilhører slægten Humicola, Trichoderma, Staphylotrichum, Aspergillus, Fusarium eller Acremonium.
9. Værtscelle ifølge krav 8, hvor den filamentøse svamp er Humicola insolens eller Trichoderma viride.
10. Fremgangsmåde til fremstilling af proteinet ifølge krav 1, hvilken fremgangsmåde omfatter trinnene: at dyrke værtscellerne ifølge et hvilket som helst af kravene 5 til 9 og at opsamle proteinet fra værtscellerne eller en kultur, der er opnået ved dyrkningen.
11. Protein fremstillet ved fremgangsmåden ifølge krav 10.
12. Cellulasepræparat omfattende proteinet ifølge krav 1 eller 11.
13. Vaskemiddelsammensætning omfattende proteinet ifølge krav 1 eller 11 eller cellulasepræparatet ifølge krav 12.
14. Fremgangsmåde til behandling af et celluloseholdigt tekstil, hvilken fremgangsmåde omfatter trinnet at bringe det celluloseholdige tekstil i kontakt med proteinet ifølge krav 1 eller 11, cellulasepræparatet ifølge krav 12 eller vaskemiddelsammensætningen ifølge krav 13.
15. Fremgangsmåde til reduktion af fnugning af et celluloseholdigt tekstil eller reduktion af en hastighed af dannelsen af fnug, omfattende trinnet at bringe det celluloseholdige tekstil i kontakt med proteinet ifølge krav 1 eller 11, cellulasepræparatet ifølge krav 12 eller vaskemiddelsammensætningen ifølge krav 13.
16. Fremgangsmåde til reduktion af vægt til forbedring af berøringsfornemmelsen og udseendet af et celluloseholdigt tekstil, omfattende trinnet at bringe det celluloseholdige tekstil i kontakt med proteinet ifølge krav 1 eller 11, cellulasepræparatet ifølge krav 12 eller vaskemiddelsammensætningen ifølge krav 13.
17. Fremgangsmåde til farveopfriskning af et farvet celluloseholdigt tekstil, omfattende trinnet at bringe det farvede celluloseholdige tekstil i kontakt med proteinet ifølge krav 1 eller 11, cellulasepræparatet ifølge krav 12 eller va- skemiddelsammensætningen ifølge krav 13.
18. Fremgangsmåde til tilvejebringelse af en lokal farveændring af farvet cel-luloseholdigt tekstil, omfattende trinnet at bringe det farvede celluloseholdige tekstil i kontakt med proteinet ifølge krav 1 eller 11, cellulasepræparatet ifølge krav 12 eller vaskemiddelsammensætningen ifølge krav 13.
19. Fremgangsmåde til reduktion af stivhed af et celluloseholdigt tekstil eller reduktion af en hastighed af dannelsen af stivhed, omfattende trinnet at bringe det celluloseholdige tekstil i kontakt med proteinet ifølge krav 1 eller 11, cellulasepræparatet ifølge krav 12 eller vaskemiddelsammensætningen ifølge krav 13.
20. Fremgangsmåde ifølge et hvilket som helst af kravene 14 til 19, hvor behandlingen af tekstilet udføres ved iblødsætning, vaskning eller skylning af tekstilet.
21. Fremgangsmåde til afsværtning af papiraffald, omfattende trinnet at behandle papiraffaldet med proteinet ifølge krav 1 eller 11 eller cellulasepræparatet ifølge krav 12 sammen med et afsværtningsmiddel.
22. Fremgangsmåde til øgning af fravær af vand i papirmasse, omfattende trinnet at behandle papirmassen med proteinet ifølge krav 1 eller 11 eller cellulasepræparatet ifølge krav 12.
23. Fremgangsmåde til forbedring af en fordøjelighed af dyrefoder, omfattende trinnet at behandle et celluloseholdigt tekstil med proteinet ifølge krav 1 eller 11 eller cellulasepræparatet ifølge krav 12.
DK04792875.9T 2003-12-03 2004-10-22 Endoglucanase stce og cellulasepræparat indeholdende samme DK1700917T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003404020 2003-12-03
PCT/JP2004/015733 WO2005054475A1 (ja) 2003-12-03 2004-10-22 エンドグルカナーゼｓｔｃｅおよびそれを含むセルラーゼ調製物

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DK1700917T3 true DK1700917T3 (da) 2016-07-25

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US (1) US7595182B2 (da)
EP (1) EP1700917B1 (da)
JP (1) JP4547335B2 (da)
CN (2) CN102766614A (da)
DK (1) DK1700917T3 (da)
ES (1) ES2575526T3 (da)
WO (1) WO2005054475A1 (da)

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