DK164794B - MEAT QUALITY PROVISION - Google Patents

MEAT QUALITY PROVISION Download PDF

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DK164794B
DK164794B DK388186A DK388186A DK164794B DK 164794 B DK164794 B DK 164794B DK 388186 A DK388186 A DK 388186A DK 388186 A DK388186 A DK 388186A DK 164794 B DK164794 B DK 164794B
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meat quality
meat
quality
animals
animal
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DK388186A (en
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Valeria Duschanek
Radomir Lasztity
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Goedoelloei Agrartudomanyi Egy
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K29/00Other apparatus for animal husbandry
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/02Breeding vertebrates
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/745Assays involving non-enzymic blood coagulation factors
    • G01N2333/75Fibrin; Fibrinogen

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  • Genetics & Genomics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Fodder In General (AREA)
  • Processing Of Meat And Fish (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)

Description

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DK 164794 BDK 164794 B

Den foreliggende opfindelse angår en fremgangsmåde til forudsigelse af kødkvalitet og til selektion på basis af kødkvalitet ved anvendelse af bestemmelse af den fibrinolytiske aktivitet.The present invention relates to a method for predicting meat quality and for selection on the basis of meat quality using determination of the fibrinolytic activity.

5 For øjeblikket anvendes i praksis halothantesten (jfr.5 At present, the halothane test is used in practice (cf.

Augustini et al., Die Fleischvirtschaft 57, 1977, s. 1028) og kreatinkinaseaktivitetstesten (Berek et al., Animal Breeding 29. 1980, s. 445) til at forudsige husdyrbestandens kødkvalitet, dvs. til at fastsætte henholdsvis stress-10 tilstanden eller den mangelfulde kødkvalitet (ved anvendelse af dyreavlseksperternes terminologi: PSE, dvs. bleg blød exsudativ; jfr. E.J. Briskey: Adv. Food Res. 13, 1964, s.Augustini et al., Die Fleischvirtschaft 57, 1977, p. 1028) and the creatine kinase activity test (Berek et al., Animal Breeding 29. 1980, p. 445) to predict the meat quality of the livestock, i.e. to determine the stress-state or the deficient meat quality, respectively (using the terminology of the animal breeding experts: PSE, i.e. pale soft exudative; cf. E.J. Briskey: Adv. Food Res. 13, 1964, p.

89 eller DFD, dvs. mørk, fast, tør, jfr. ibid 13, 1964, s.89 or DFD, i.e. dark, firm, dry, cf. ibid 13, 1964, p.

89.) 15 Princippet for halothantesten, som kun anvendes til at vurdere stresstilstanden hos svin, består i undersøgelse af, om svinet, som er bedøvet med halothan, sover i en afslappet eller i en spastisk tilstand. Dyrene, som er faldet i søvn i en spastisk tilstand, og som derfor er stressfølsom-20 me, udelukkes gradvis fra videre avl; denne metode er nyttig til undersøgelse af stressfølsomheden, hvorimod kødkvaliteten imidlertid ikke utvetydigt kan forudses ved denne metode. En yderligere væsentlig ulempe består i, at denne metode er farlig, eftersom 4 til 5 ud af 100 svin går til 25 inden for ét eksperiment.89.) 15 The principle of the halothane test, which is used only to assess the state of stress in pigs, consists in examining whether the pig, which is anesthetized with halothane, sleeps in a relaxed or in a spastic state. The animals which have fallen asleep in a spastic state, and which are therefore sensitive to stress, are gradually excluded from further breeding; this method is useful for the study of stress sensitivity, whereas the meat quality, however, can not be unambiguously predicted by this method. A further significant disadvantage is that this method is dangerous, since 4 to 5 out of 100 pigs go to 25 within one experiment.

Princippet i kreatinkinaseaktivitetstesten er at tage en blodprøve fra dyrets vene, bestemme aktiviteten af kreatin-kinase og derudfra drage en konklusion angående stressfølsomheden og derved indirekte angående dyrets kødkvalitet.The principle of the creatine kinase activity test is to take a blood sample from the animal's vein, determine the activity of creatine kinase and from that draw a conclusion regarding the stress sensitivity and thereby indirectly regarding the animal's meat quality.

30 Ifølge ophavsmændene til denne testmetode er niveauet for korrelationen mellem kreatinkinaseaktiviteten og kødkvaliteten ikke højere end 49% (dvs. den gode kødkvalitet kan kun forudsiges for 49 ud af 100 dyr) . Denne metode er således ikke tilstrækkeligt pålidelig.30 According to the authors of this test method, the level of the correlation between creatine kinase activity and meat quality is not higher than 49% (ie good meat quality can only be predicted for 49 out of 100 animals). Thus, this method is not sufficiently reliable.

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2 P. Sellier (Slaughter Animal and Flesh Production 10. 1980, s. 42) beskriver en metode til forudsigelse af dyrs stress-følsomhed. Princippet i denne metode er, at tilstedeværelsen af HAL3-genet påvises, hvilket er karakteristisk for 5 stressfølsomme dyr. Denne metode er nyttig til at verificere den biologiske arvelighed af stressfølsomhed, men kan ikke med god sandsynlighed anvendes til at forudsige kødkvaliteten.2 P. Sellier (Slaughter Animal and Flesh Production 10. 1980, p. 42) describes a method for predicting animal stress sensitivity. The principle of this method is that the presence of the HAL3 gene is detected, which is characteristic of 5 stress-sensitive animals. This method is useful for verifying the biological heritability of stress sensitivity, but cannot in all probability be used to predict meat quality.

For fuldstændighedens skyld skal det bemærkes, at i den 10 industrielle praksis anvendes pH-bestemmelsen i en defineret muskelgruppe til at kvalificere kødet fra de slagtede dyr. Denne metode er imidlertid kun nyttig til en teknologisk kødselektion og kan tydeligvis ikke anvendes til forudsigelse.For the sake of completeness, it should be noted that in industrial practice, the pH determination in a defined muscle group is used to qualify the meat from the slaughtered animals. However, this method is only useful for a technological meat selection and clearly cannot be used for prediction.

15 For øjeblikket anvendes bestemmelse af den fibrinolytiske aktivitet i medicinsk praksis til undersøgelse af meget sjældne (enestående) kliniske billeder, fx fravær af en blodfaktor.At present, determination of fibrinolytic activity is used in medical practice to examine very rare (unique) clinical pictures, such as the absence of a blood factor.

Opfindelsen sigter mod elaboration af en metode, ved anven-20 delse af hvilken kødkvaliteten af husdyrbestanden kan forudsiges, og hvorved de dyr, som er egnede til videre avl, kan selekteres fra de stressfølsomme dyr, som giver en mangelfuld kødkvalitet.The invention aims at elaboration of a method, using which the meat quality of the livestock can be predicted, and whereby the animals which are suitable for further breeding can be selected from the stress-sensitive animals which give a deficient meat quality.

Det har vist sig, at ved at måle den fibrinolytiske aktivi-25 tet i det blod, som er udtaget fra husdyrbestanden, og ved derfra at bestemme lyse-procenten forekommer der en tærskelværdi for lyse-procenten, som afhænger af arten og sorten af dyret, på basis af hvilken husdyrbestanden kan klassificeres ifølge de ovennævnte synspunkter, dvs. de dyr, 30 der giver en god kødkvalitet, kan selekteres fra dem, der giver en utilstrækkelig kvalitet, og på denne måde kan kvaliteten af kødet forudsiges.It has been found that by measuring the fibrinolytic activity in the blood taken from the livestock population and by determining the light percentage therefrom, there is a threshold value for the light percentage which depends on the species and variety of the animal. , on the basis of which the livestock population can be classified according to the above views, ie. the animals which give a good quality of meat can be selected from those which give an insufficient quality, and in this way the quality of the meat can be predicted.

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Opfindelsen angår således en metode til forudsigelse af kødkvaliteten hos husdyrbestande og til selektion på basis af kødkvaliteten.The invention thus relates to a method for predicting the meat quality of livestock and for selection on the basis of the meat quality.

I overensstemmelse med opfindelsen måles den fibrinolytiske 5 aktivitet i frisk blod udtaget fra husdyrbestanden, lyseprocenten bestemmes derfra, hvorpå de dyr, som viser en lyse-procent, der er lavere end en tærskelværdi, som afhænger af arten og sorten af dyret, videreavles, hvorimod de dyr, der udviser en lyse-procent, som er højere end denne 10 tærskelværdi, straks eller gradvis udelukkes fra avlen.In accordance with the invention, the fibrinolytic activity in fresh blood taken from the livestock population is measured, the light percentage being determined therefrom, whereupon the animals which show a light percentage lower than a threshold value depending on the species and variety of the animal are bred, whereas those animals that show a brightness percentage higher than this 10 threshold value are immediately or gradually excluded from breeding.

I praksis udføres fremgangsmåden ifølge opfindelsen på følgende måde:In practice, the method according to the invention is carried out as follows:

For det første bør det bemærkes, at i princippet kan metoden ifølge opfindelsen anvendes på en hvilken som helst 15 husdyrbestand, forudsat at det biokemiske grundlag for opfindelsen tages i betragtning. I praksis har opfindelsen imidlertid særlig betydning i avl af svin, kvæg, heste, fjerkræ, får og kaniner.First, it should be noted that, in principle, the method of the invention can be applied to any livestock population, provided that the biochemical basis of the invention is taken into account. In practice, however, the invention is of particular importance in the breeding of pigs, cattle, horses, poultry, sheep and rabbits.

Blodprøven tages hensigtsmæssigt fra øre- eller jugular-20 venen i husdyret under omstændigheder, som muliggør, at blodprøven ikke undergår nogen hæmolyse. For at udtage blodprøven anvendes hensigtsmæssigt en sprøjte, hvorimod et medium, som indeholder et antikoagulansmiddel, fx en tri-natriumcitratopløsning på 3,8%, anvendes til opsamling af 25 blodprøven.The blood sample is conveniently taken from the ear or jugular vein of the domestic animal under circumstances which allow the blood sample to undergo no hemolysis. To take the blood sample, a syringe is suitably used, whereas a medium containing an anticoagulant, eg a 3.8% trisodium citrate solution, is used to collect the blood sample.

Den fibrinolytiske aktivitet bestemmes i den udtagne blodprøve som beskrevet ovenfor ved anvendelse af en i og for sig kendt metode, fx ifølge Fearnley (Clin. Sci. 16. 1957, s. 646). Lyse-procenten, som er karakteristisk for aktivi-30 teten, opnås ved at sammenligne den målte værdi med en standardværdi.The fibrinolytic activity is determined in the blood sample taken as described above using a method known per se, for example according to Fearnley (Clin. Sci. 16. 1957, p. 646). The brightness percentage characteristic of the activity is obtained by comparing the measured value with a default value.

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4 I betragtning af variabiliteten i den biokemiske tilstand hos husdyrbestanden er det hensigtsmæssigt at bestemme lyse-procenten tre gange ud fra blodprøver, som gentagne gange udtages i 3 dage under en minimal stresstilstand.Given the variability in the biochemical status of the livestock population, it is appropriate to determine the light percentage three times from blood samples, which are repeatedly taken for 3 days under a minimal stress condition.

5 Tærskelværdien for lyse-procenten er ca. 30% for svin; det er karakteristisk for arten og sorten; den kan imidlertid variere i overensstemmelse med stammen.5 The threshold value for the light percentage is approx. 30% for pigs; it is characteristic of the species and variety; however, it may vary according to the strain.

I det følgende vil de områder inden for dyreavlen og den kødfremstillende industri, hvor fremgangsmåden ifølge op-10 findelsen kan anvendes effektivt, blive anført.In the following, the areas in animal husbandry and the meat-producing industry where the method according to the invention can be used effectively will be listed.

Under anvendelse af fremgangsmåden ifølge opfindelsen kan kødkvaliteten hos husdyrbestanden forudsiges. Dette betyder, at efter måling af den fibrinolytiske aktivitet ved fremgangsmåden ifølge opfindelsen og efter bestemmelse af 15 lyse-procenten derfra kan det forudsiges, om der efter slagtning af husdyrbestanden opnås kød af PSE-kvalitet (dvs. af dårlig kvalitet) eller kød af DFD-kvalitet (dvs. normalt kød af god kvalitet).Using the method according to the invention, the meat quality of the livestock population can be predicted. This means that after measuring the fibrinolytic activity by the method according to the invention and after determining the 15% of light therefrom, it can be predicted whether, after slaughter of the livestock, PSE-grade meat (i.e. of poor quality) or meat of DFD is obtained. quality (ie usually good quality meat).

Fremgangsmåden ifølge opfindelsen til forudsigelse af 20 kødkvaliteten viser for svin en korrelation på 71,1% med kødkvaliteten bestemt ved den traditionelle metode, som er baseret på pH og pH24-værdier i kødet fra slagtedyr (R.The method according to the invention for predicting the meat quality shows for pigs a correlation of 71.1% with the meat quality determined by the traditional method, which is based on pH and pH24 values in the meat from slaughter animals (R.

Hamm og R. Potthast: Die Fleischwirtschaft 52, 1972, s.Hamm and R. Potthast: Die Fleischwirtschaft 52, 1972, p.

200), hvorimod denne korrelation ikke er mere end 49% ved 25 anvendelse af kreatinkinaseaktivitetstesten, som er beskrevet ovenfor under den kendte teknik på området. Det er klart, at kødkvaliteten kan forudsiges signifikant på en mere pålidelig måde under anvendelse af fremgangsmåden ifølge opfindelsen, hvilket har den største betydning set 30 ud fra slagteripraksis, eftersom den gør det muligt at selektere de dyr, som giver kød af en mangelfuld (dårlig) kvalitet, og at oparbejde dyr i overensstemmelse med kødkvaliteten. Ved at anvende fremgangsmåden ifølge opfindel-200), whereas this correlation is not more than 49% using the creatine kinase activity test described above in the prior art. It is clear that the meat quality can be significantly predicted in a more reliable way using the method according to the invention, which has the greatest significance in terms of slaughterhouse practice, since it makes it possible to select the animals which give meat of a deficient (poor ) quality, and to process animals in accordance with the quality of the meat. By using the method according to the invention

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5 sen kan den procedure, hvorved der udvælges på grundlag af kødstykker efter slagtning, undlades.5 late, the procedure for selecting on the basis of pieces of meat after slaughter may be omitted.

Et andet område for anvendelse af fremgangsmåden ifølge opfindelsen er den fortsatte forbedring af kødkvaliteten i 5 en afgrænset population og i avlen af en stress-resistent population med normal kødkvalitet. Ved således at anvende fremgangsmåden ifølge opfindelsen til den fortsatte selektion af avlsdyr i en afgrænset population vil kødkvaliteten i afkommet fortsat forbedres. Halothantesten, som er nævnt 10 ovenfor i beskrivelsen af den kendte teknik på området, er kun nyttig til screening for stressfølsomhed, og ved anvendelse af denne test kan der ikke opnås en fraskillelse af dyr med en dårlig kødkvalitet, idet en dårlig kødkvalitet selv hos stress-resistente dyr kan opstå som følge af 15 enzyminduktionsegenskaber ved denne test.Another area of application of the method according to the invention is the continuous improvement of the meat quality in a limited population and in the breeding of a stress-resistant population with normal meat quality. Thus, by applying the method according to the invention to the continued selection of breeding animals in a limited population, the meat quality in the offspring will continue to be improved. The halothane test mentioned above in the description of the prior art is only useful for screening for stress sensitivity, and by using this test a separation of animals with a poor meat quality can not be achieved, as a poor meat quality even in stress -resistant animals can occur due to 15 enzyme induction properties in this test.

Hvis der ifølge fremgangsmåden ifølge opfindelsen etableres en population med forbedret kødkvalitet under anvendelse af den selektion, der er baseret på kødkvaliteten, kan det forventes, at andre betydningsfulde egenskaber hos denne 20 population såsom stress-resistensen, fødeudnyttelsesgraden og endog de generelle genetiske egenskaber vil forbedres.If, according to the method of the invention, a population with improved meat quality is established using the selection based on the meat quality, it can be expected that other important characteristics of this population such as stress resistance, food utilization rate and even the general genetic characteristics will be improved. .

Det ses således, at en forbedring af beskaffenheden som helhed tilvejebringes ved selektionen ved fremgangsmåden ifølge opfindelsen i tilgift til forudsigelsen af kødkvali-25 teten og til den øgede forbedring af kødkvaliteten inden for en afgrænset population.It is thus seen that an improvement of the condition as a whole is provided by the selection by the method according to the invention in addition to the prediction of the meat quality and to the increased improvement of the meat quality within a limited population.

Fremgangsmåden ifølge opfindelsen tilvejebringer hjælp til at løse op for modsætningen mellem kvantiteten og kvaliteten af kødet, hvilket er et problem i avlen af opfedede 30 dyr, eftersom denne metode gør det muligt at finde et økonomisk optimum i kødproduktionen.The method according to the invention provides help in solving the contradiction between the quantity and the quality of the meat, which is a problem in the breeding of fattened animals, since this method makes it possible to find an economical optimum in the meat production.

Andre fordele ved fremgangsmåden ifølge opfindelsen er indlysende på basis af de ovenfor beskrevne kendsgerninger.Other advantages of the method according to the invention are obvious on the basis of the facts described above.

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Fremgangsmåden ifølge opfindelsen belyses nærmere ved nedenstående eksempel:The process according to the invention is further illustrated by the following example:

EKSEMPELEXAMPLE

Fra grupper, som hver består af 100 svin med en hybridsam-5 mensætning fra "Hungarian great white" og "Dutch plain" bestemt til skinkeproduktion i slagteriet, udvælges vilkårligt 10-12 svin, og der tages på slagtningstidspunktet en blodprøve fra hvert dyr i en 3,8%'s trinatriumcitratopløs-ning. I de således indsamlede prøver bestemmes den fibrino-10 lytiske aktivitet under anvendelse af Fearnleys metode, som er beskrevet ovenfor, og samtidig udføres der efter slagtning pHi- og pH24-målinger på de udvalgte svin under anvendelse af den metode, der er beskrevet i den ovenfor nævnte litteratur; eller der udføres en G0F024-farvebestemmelse 15 (jfr. J. Dohy: Animal Breeding Genetics, 1979, s. 133, 153 og 200, red. Mezogazdasågi Kiadd, Budapest), og kødkvaliteten bestemmes på basis af disse målinger. Den fibrinolytiske aktivitet beregnes ved at sammenligne den opnåede værdi med standardværdien, og korrelationen mellem den således 20 opnåede lyse-procent og den kødkvalitet, der er bestemt under anvendelse af de traditionelle metoder, fastlægges. Kødkvaliteten bestemt på basis af lyse-procenten ifølge opfindelsen viser en korrelation på 71,1% med kødkvaliteten bestemt på basis af pH-L-værdien, hvorimod denne korrelation 25 er 67% med den kødkvalitet, der er målt under anvendelse af pH^- og pH24-værdierne, og 68,5% med kødkvaliteten bestemt ved anvendelse af G0F024-værdien. Korrelationen er 56% med kødkvaliteten bestemt ved anvendelse af kreatinkinaseakti-vitetstestmetoden. Det ses klart, at fremgangsmåden ifølge 30 opfindelsen på den ene side har god korrelation med kendte metoder, hvis ulemper er blevet beskrevet ovenfor; og på den anden måde er metoden ifølge opfindelsen per se mere nyttig til bestemmelse af kødkvaliteten.From groups each consisting of 100 pigs with a hybrid composition from "Hungarian great white" and "Dutch plain" intended for ham production in the slaughterhouse, 10-12 pigs are randomly selected and a blood sample is taken from each animal at the time of slaughter. a 3.8% trisodium citrate solution. In the samples thus collected, the fibrinolytic activity is determined using Fearnley's method described above, and at the same time, after slaughter, pH and pH24 measurements are performed on the selected pigs using the method described in the literature mentioned above; or a G0F024 color determination 15 is performed (cf. J. Dohy: Animal Breeding Genetics, 1979, pp. 133, 153 and 200, eds. Mezogazdasågi Kiadd, Budapest) and the meat quality is determined on the basis of these measurements. The fibrinolytic activity is calculated by comparing the value obtained with the standard value, and the correlation between the light percentage thus obtained and the meat quality determined using the traditional methods is determined. The meat quality determined on the basis of the light percentage according to the invention shows a correlation of 71.1% with the meat quality determined on the basis of the pH-L value, whereas this correlation is 67% with the meat quality measured using pH and the pH24 values, and 68.5% with the meat quality determined using the G0F024 value. The correlation is 56% with the meat quality determined using the creatine kinase activity test method. It is clear that the method according to the invention on the one hand has a good correlation with known methods, the disadvantages of which have been described above; and otherwise the method according to the invention is per se more useful for determining the quality of the meat.

Claims (2)

1. Fremgangsmåde til forudsigelse af en husdyrbestands kødkvalitet og til selektion baseret på kødkvaliteten, kendetegnet ved, at den fibrinolytiske aktivitet 5. frisk blod udtaget fra husdyrbestanden måles, lyse-procenten bestemmes derudfra, de dyr, der viser en lyse-procent lavere end en tærskelværdi, som er afhængig af arten og sorten af dyret, videreavles, hvorimod de dyr, der viser en lyse-procent, som er højere end denne tærskelværdi, 10 straks eller gradvis udelukkes fra avlen.Method for predicting the meat quality of a livestock population and for selection based on meat quality, characterized in that the fibrinolytic activity 5. fresh blood taken from the livestock population is measured, the light percentage is determined from there, the animals showing a light percentage lower than one threshold value, which depends on the species and variety of the animal, is further bred, whereas those animals which show a brightness percentage higher than this threshold are immediately or gradually excluded from breeding. 2. Fremgangsmåde ifølge krav 1, kendetegnet ved, at 30% anvendes som tærskelværdi for lyse-procenten for svin.Process according to Claim 1, characterized in that 30% is used as the threshold value for the light percentage for pigs.
DK388186A 1985-08-15 1986-08-14 MEAT QUALITY PROVISION DK164794C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HU314785 1985-08-15
HU853147A HU193126B (en) 1985-08-15 1985-08-15 Method for forecasting quality of flesh of the livestocks and for selecting livestocks on the basis of this

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DK388186D0 DK388186D0 (en) 1986-08-14
DK388186A DK388186A (en) 1987-02-16
DK164794B true DK164794B (en) 1992-08-17
DK164794C DK164794C (en) 1993-01-04

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CN105606778B (en) * 2016-03-07 2018-03-23 浙江工商大学 A kind of live pig white muscles (PSE meat) quick determination method

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JPS6269941A (en) 1987-03-31
FR2586165B1 (en) 1989-12-22
DK388186A (en) 1987-02-16
FI863323A (en) 1987-02-16
FI863323A0 (en) 1986-08-15
DK164794C (en) 1993-01-04
CN86105263A (en) 1987-07-01
SE464783B (en) 1991-06-10
FR2586165A1 (en) 1987-02-20
DK388186D0 (en) 1986-08-14
HU193126B (en) 1987-08-28
NO863280D0 (en) 1986-08-14
DE3624048A1 (en) 1987-02-19
GB2179150A (en) 1987-02-25
CH672027A5 (en) 1989-10-13
NO170907C (en) 1992-12-23
SE8603423L (en) 1987-02-16
BE905268A (en) 1986-12-01
NO170907B (en) 1992-09-14
ATA219786A (en) 1993-12-15
GB8619834D0 (en) 1986-09-24
NO863280L (en) 1987-02-16
NL8602064A (en) 1987-03-02
SE8603423D0 (en) 1986-08-14
GB2179150B (en) 1989-09-20

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