DK1394173T3 - Forbedret fremgangsmåde til bisulfitbehandling - Google Patents

Forbedret fremgangsmåde til bisulfitbehandling

Info

Publication number
DK1394173T3
DK1394173T3 DK03019321T DK03019321T DK1394173T3 DK 1394173 T3 DK1394173 T3 DK 1394173T3 DK 03019321 T DK03019321 T DK 03019321T DK 03019321 T DK03019321 T DK 03019321T DK 1394173 T3 DK1394173 T3 DK 1394173T3
Authority
DK
Denmark
Prior art keywords
solid phase
glass
nucleic acid
bisulfite
improved method
Prior art date
Application number
DK03019321T
Other languages
Danish (da)
English (en)
Inventor
Christine Dr Markert-Hahn
Dirk Dr Block
Original Assignee
Hoffmann La Roche
Epigenomics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32071078&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1394173(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from EP02019097A external-priority patent/EP1394172A1/de
Application filed by Hoffmann La Roche, Epigenomics Ag filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK1394173T3 publication Critical patent/DK1394173T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Compounds Of Iron (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
DK03019321T 2002-08-29 2003-08-27 Forbedret fremgangsmåde til bisulfitbehandling DK1394173T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02019097A EP1394172A1 (de) 2002-08-29 2002-08-29 Verbessertes Verfahren Zur Bisulfit-Behandlung
EP02028114 2002-12-18

Publications (1)

Publication Number Publication Date
DK1394173T3 true DK1394173T3 (da) 2008-02-04

Family

ID=32071078

Family Applications (2)

Application Number Title Priority Date Filing Date
DK03019321T DK1394173T3 (da) 2002-08-29 2003-08-27 Forbedret fremgangsmåde til bisulfitbehandling
DK07012188.4T DK1829886T3 (en) 2002-08-29 2003-08-27 IMPROVED BISULPHITE TREATMENT PROCEDURE

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK07012188.4T DK1829886T3 (en) 2002-08-29 2003-08-27 IMPROVED BISULPHITE TREATMENT PROCEDURE

Country Status (15)

Country Link
US (2) US9394332B2 (de)
EP (2) EP1829886B1 (de)
JP (2) JP5153048B2 (de)
CN (1) CN1271081C (de)
AT (1) ATE374782T1 (de)
AU (1) AU2003236461B2 (de)
CA (1) CA2438327C (de)
CY (1) CY1107118T1 (de)
DE (1) DE60316642T2 (de)
DK (2) DK1394173T3 (de)
ES (2) ES2545577T3 (de)
HK (1) HK1062688A1 (de)
HU (1) HUE027240T2 (de)
PT (1) PT1394173E (de)
SI (2) SI1829886T1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10154317B4 (de) * 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
US9394332B2 (en) * 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
US7262013B2 (en) * 2003-08-29 2007-08-28 Applera Corporation Bisulfite method
US7368239B2 (en) * 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
US7534873B2 (en) * 2003-08-29 2009-05-19 Applied Biosystems, Llc Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
US7371526B2 (en) * 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
CA2540310A1 (en) 2003-10-09 2005-04-28 Epigenomics Ag Improved bisulfite conversion of dna
US20080070240A2 (en) * 2004-04-08 2008-03-20 Toyo Boseki Kabushiki Kaisha Composition for deaminating dna and method of detecting methylated dna
EP1632578A1 (de) * 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
CA2581140A1 (en) * 2004-09-21 2006-03-30 Applera Corporation Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated dna analysis
US7658288B2 (en) 2004-11-08 2010-02-09 Applied Biosystems, Llc Bisulfite conversion reagent
EP2385136A1 (de) 2005-04-01 2011-11-09 Epigenomics AG Verbesserte Bisulfit-Umwandlung von DNA
EP1963352B1 (de) * 2005-12-14 2010-09-08 Roche Diagnostics GmbH Neues verfahren für die bisulfitbehandlung
US7820385B2 (en) * 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
WO2009088987A2 (en) * 2008-01-03 2009-07-16 The Johns Hopkins University Compositions and methods for polynucleotide extraction and methylation detection
US9115386B2 (en) 2008-09-26 2015-08-25 Children's Medical Center Corporation Selective oxidation of 5-methylcytosine by TET-family proteins
FR2940805B1 (fr) 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
EP3141616B1 (de) 2011-07-29 2020-05-13 Cambridge Epigenetix Limited Methoden zum nachweis der nukleotidmodifikation
ES2669512T3 (es) 2012-11-30 2018-05-28 Cambridge Epigenetix Limited Agente oxidante para nucleótidos modificados
EP3782731A1 (de) * 2015-06-10 2021-02-24 Biocartis N.V. Verbesserte detektion von methylierter dna
US11603555B2 (en) 2015-06-15 2023-03-14 Cepheid Integrated purification and measurement of DNA methylation and co-measurement of mutations and/or MRNA expression levels in an automated reaction cartridge
MX2019006628A (es) 2016-12-12 2019-11-12 Cepheid Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado.
CN108265050A (zh) * 2018-03-13 2018-07-10 普迈德（北京）科技有限公司 一种血浆直接亚硫酸氢盐转化的方法及其应用
CA3111887A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
DE102019118332B4 (de) 2019-07-07 2022-04-07 Ist Innuscreen Gmbh Verfahren und testkit zur bisulfitmodifizierung von dna
CN114317761A (zh) * 2022-02-23 2022-04-12 厦门飞朔生物技术有限公司 一种宫颈癌相关基因甲基化检测方法

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK171161B1 (da) 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
DE3724442A1 (de) 1987-07-23 1989-02-02 Europ Lab Molekularbiolog Verfahren und vorrichtung zur reinigung von m-13-phagen-dna
IE61148B1 (en) 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
EP0425563B1 (de) 1988-07-20 1996-05-15 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
CA2003508C (en) 1988-11-21 2000-01-25 Erik Hornes Monodisperse superparamagnetic particles for use in nucleic acid analyses
US5639611A (en) 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5137806A (en) 1989-12-11 1992-08-11 Board Of Regents, The University Of Texas System Methods and compositions for the detection of sequences in selected DNA molecules
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
CA2102264C (en) 1992-11-13 2000-08-01 Daniel Lee Woodard Boron silicates, aluminum silicates, phosphosilicates and purification of dna
US5438127A (en) 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane
US5503816A (en) 1993-09-27 1996-04-02 Becton Dickinson And Company Silicate compounds for DNA purification
US5552277A (en) 1994-07-19 1996-09-03 The Johns Hopkins University School Of Medicine Genetic diagnosis of prostate cancer
DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
NZ502323A (en) 1996-06-04 2001-09-28 Univ Utah Res Found Monitoring a fluorescence energy transfer pair during hybridization of first probe labelled with fluorescein to second probe labelled with Cy5 or Cy5.5
WO1998046797A1 (en) * 1997-04-16 1998-10-22 Immunological Associates Of Denver Nucleic acid archiving
GB9714716D0 (en) * 1997-07-11 1997-09-17 Brax Genomics Ltd Characterising nucleic acids
DE19743518A1 (de) 1997-10-01 1999-04-15 Roche Diagnostics Gmbh Automatisierbare universell anwendbare Probenvorbereitungsmethode
DE59912604D1 (de) 1998-02-04 2005-11-03 Merck Patent Gmbh Verfahren zur isolierung und aufreinigung von nucleinsäuren
PT1144620E (pt) 1998-11-30 2004-01-30 Roche Diagnostics Gmbh Perticulas magneticas para a purificacao de acidos nucleicos
DE19856064C2 (de) 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US6423536B1 (en) 1999-08-02 2002-07-23 Molecular Dynamics, Inc. Low volume chemical and biochemical reaction system
PT1232502E (pt) * 1999-11-17 2006-05-31 Roche Diagnostics Gmbh Particulas de vidro magneticas, metodo para a sua preparacao e suas utilizacoes
DE19958042A1 (de) 1999-12-03 2001-06-21 Invitek Gmbh Oberflächenmodifizierte Trägermaterialien zur Bindung biologischer Materialien, Verfahren zu ihrer Herstellung und ihre Verwendung
DE10029915B4 (de) 2000-06-19 2005-06-16 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
DE10050942B4 (de) 2000-10-10 2005-11-17 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
US20030152950A1 (en) 2001-06-27 2003-08-14 Garner Harold R. Identification of chemically modified polymers
AU2002337660A1 (en) * 2001-07-31 2003-02-17 Claude Gagna Methods for immobilizing molecules to a solid phase and uses thereof
DE10154317B4 (de) 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
US9394332B2 (en) * 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
EP1394172A1 (de) 2002-08-29 2004-03-03 Boehringer Mannheim Gmbh Verbessertes Verfahren Zur Bisulfit-Behandlung
CA2547743C (en) * 2003-12-02 2011-06-28 F. Hoffmann-La Roche Ag Improved method for bisulfite treatment
EP1963352B1 (de) * 2005-12-14 2010-09-08 Roche Diagnostics GmbH Neues verfahren für die bisulfitbehandlung

Also Published As

Publication number Publication date
EP1829886B1 (de) 2015-07-29
ES2545577T3 (es) 2015-09-14
EP1394173A1 (de) 2004-03-03
JP2004089195A (ja) 2004-03-25
CN1271081C (zh) 2006-08-23
ES2294231T3 (es) 2008-04-01
CA2438327A1 (en) 2004-02-29
US20160068888A1 (en) 2016-03-10
HK1062688A1 (en) 2004-11-19
CA2438327C (en) 2008-04-15
SI1829886T1 (sl) 2015-12-31
US20040241704A1 (en) 2004-12-02
DE60316642D1 (de) 2007-11-15
AU2003236461B2 (en) 2009-05-28
EP1394173B1 (de) 2007-10-03
JP5310059B2 (ja) 2013-10-09
US9394332B2 (en) 2016-07-19
SI1394173T1 (sl) 2008-02-29
JP2009106304A (ja) 2009-05-21
ATE374782T1 (de) 2007-10-15
DK1829886T3 (en) 2015-08-17
PT1394173E (pt) 2007-11-28
CN1495192A (zh) 2004-05-12
EP1394173B9 (de) 2008-10-15
CY1107118T1 (el) 2012-10-24
HUE027240T2 (en) 2016-10-28
DE60316642T2 (de) 2008-01-31
AU2003236461A1 (en) 2004-03-18
US9868756B2 (en) 2018-01-16
JP5153048B2 (ja) 2013-02-27
EP1829886A2 (de) 2007-09-05
EP1829886A3 (de) 2008-10-15

Similar Documents

Publication Publication Date Title
DK1394173T3 (da) Forbedret fremgangsmåde til bisulfitbehandling
DE60238493D1 (de) Is von seltenen zellen verwendete markierte zellen
CA2631829A1 (en) New method for bisulfite treatment
EP1590362B8 (de) Verbessertes Verfahren zur Behandlung durch Bisulfit
EP2088210A3 (de) Verbesserte Bisulfit-Umwandlung von DNA
DE60230672D1 (de) Nukleinsäureanreicherung
AU785425C (en) Methods of genomic analysis
AU6380001A (en) Porous ferro- or ferrimagnetic glass particles for isolating molecules
IS6515A (is) Fósturstofnfrumurækt prímata, sem laus er við sermi
AU2003241311A1 (en) Simple catalytic dna biosensors for ions based on color changes
AU2001287396A1 (en) Nucleic acid molecules, methods and kits for selecting a nucleic acid having a desired feature
WO2006024541A3 (en) Dna decontamination method
AU2002363259A1 (en) Method for the detection of cytosine methylation in immobilised DNA samples
AU2003303588A1 (en) Methods and compositions for sequencing nucleic acid molecules
AU2001268232A1 (en) Compositions, methods, and kits relating to resistin-like molecules
WO2003016550A3 (en) Compositions and methods comprising control nucleic acid
AU1085401A (en) Genotyping reagents, kits and methods of use thereof
WO2003048332A3 (en) METHODS FOR ANALYZING METHYLATED CpG ISLANDS AND GC RICH REGIONS
AUPS064202A0 (en) Improved method for the analysis of nucleic acid samples
AU2002249148A1 (en) Analysis of methylation patterns in genomic dna using bisulfite treatment and fluorescence polarisation assay techniques
DE60135751D1 (de) Methode zum nachweis von nukleinsäurepolymorphismen
AU2002306860A1 (en) Isolated human nadph oxidase, nucleic acid molecules encoding said proteins, and uses thereof
WO2003035844A3 (en) Novel histone methyltransferase and methods of its use
AU2001286722A1 (en) Methods for reducing complexity of nucleic acid samples
AU2002312265A1 (en) Modified microporous membrane for non-specific and sequence-specific nucleic acid capture and methods of use