DK1078081T3 - Fremgangsmåde til fremstilling af heterologe polypeptider i trichothecen deficiente filamentöse svampe mutantceller - Google Patents

Fremgangsmåde til fremstilling af heterologe polypeptider i trichothecen deficiente filamentöse svampe mutantceller

Info

Publication number
DK1078081T3
DK1078081T3 DK99925717T DK99925717T DK1078081T3 DK 1078081 T3 DK1078081 T3 DK 1078081T3 DK 99925717 T DK99925717 T DK 99925717T DK 99925717 T DK99925717 T DK 99925717T DK 1078081 T3 DK1078081 T3 DK 1078081T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
filamentous fungal
mutant
cells
trichothecene
Prior art date
Application number
DK99925717T
Other languages
Danish (da)
English (en)
Inventor
John C Royer
Lynne M Christianson
Gregory A Gambetta
Howard Brody
Suzanne M Otani
Wendy T Yoder
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of DK1078081T3 publication Critical patent/DK1078081T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03006—Trichodiene synthase (4.2.3.6)
DK99925717T 1998-05-20 1999-05-20 Fremgangsmåde til fremstilling af heterologe polypeptider i trichothecen deficiente filamentöse svampe mutantceller DK1078081T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8221798A 1998-05-20 1998-05-20
PCT/US1999/011223 WO1999060137A2 (fr) 1998-05-20 1999-05-20 Procedes de production de polypeptides heterologues dans des cellules mutantes de champignons filamenteuxdeficientes en trichothecene

Publications (1)

Publication Number Publication Date
DK1078081T3 true DK1078081T3 (da) 2008-08-04

Family

ID=22169802

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99925717T DK1078081T3 (da) 1998-05-20 1999-05-20 Fremgangsmåde til fremstilling af heterologe polypeptider i trichothecen deficiente filamentöse svampe mutantceller

Country Status (9)

Country Link
US (3) US6180366B1 (fr)
EP (1) EP1078081B1 (fr)
JP (2) JP2002515253A (fr)
CN (2) CN1190495C (fr)
AT (1) ATE395424T1 (fr)
AU (1) AU4195099A (fr)
DE (1) DE69938722D1 (fr)
DK (1) DK1078081T3 (fr)
WO (1) WO1999060137A2 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
EP1174157B1 (fr) * 1998-04-27 2005-06-29 Surmodics Inc. Revêtement destiné à libérer des agents bioactifs
JP2002515253A (ja) * 1998-05-20 2002-05-28 ノボザイムス バイオテック，インコーポレイティド トリコテセン−欠失糸状菌変異体細胞において異種ポリベプチドを生成するための方法
FR2826978B1 (fr) * 2001-07-06 2004-09-17 Agronomique Inst Nat Rech Procede de determination de la capacite de fusarium a produire des toxines trichotecenes
US8148155B2 (en) * 2002-04-22 2012-04-03 Novozymes, Inc. Methods for increasing homologous recombination of a nucleic acid sequence
US20060240509A1 (en) * 2002-08-30 2006-10-26 Jean-Luc Jonniaux Myrothecium sp transformation and expression system
DE60328802D1 (de) 2002-08-30 2009-09-24 Novozymes Inc Verfahren zur herstellung von säugetier-trypsinen
US7368262B2 (en) 2002-11-18 2008-05-06 Novozymes, Inc. Promoter variants for expressing genes in a fungal cell
WO2007122249A1 (fr) * 2006-04-25 2007-11-01 Dsm Ip Assets B.V. Production de composé dans un hôte recombiné
BRPI0711709A2 (pt) * 2006-05-18 2011-11-29 Koninkl Philips Electronics Nv dispositivo para tostar alimento
WO2010039840A1 (fr) * 2008-09-30 2010-04-08 Novozymes, Inc. Procédés de production de polypeptides chez des mutants de fusarium venenatum déficients en enzymes
US20100316764A1 (en) * 2009-06-10 2010-12-16 Engrain, LLC Flour supplement compositions and methods for preparing wheat flour
US20120184008A1 (en) * 2009-08-05 2012-07-19 Novozymes A/S Filamentous fungi and methods for producing trichodiene from lignocellulosic feedstocks
WO2011075677A2 (fr) 2009-12-18 2011-06-23 Novozymes, Inc. Procédés de production de polypeptides dans des mutants de trichoderma présentant une carence en protéase
CN108588060B (zh) * 2017-03-07 2020-12-01 武汉康复得生物科技股份有限公司 一种用丝状真菌宿主细胞表达的重组草酸脱羧酶
JP2021078354A (ja) * 2018-03-08 2021-05-27 有限会社アルティザイム・インターナショナル フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質
CN112522231B (zh) * 2020-12-14 2023-03-14 广东省微生物研究所（广东省微生物分析检测中心） 一种酰基转移酶及其编码基因和应用
WO2023088791A1 (fr) 2021-11-22 2023-05-25 Basf Se Souches de paenibacillus produisant de faibles quantités de composés comprenant du dab

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8904762D0 (en) * 1989-03-02 1989-04-12 Glaxo Group Ltd Biological process
AU2705895A (en) * 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5594119A (en) * 1994-09-20 1997-01-14 Novo Nordisk A/S Gene encoding carboxypeptidase of aspergillus niger
TW555853B (en) * 1996-03-11 2003-10-01 Meiji Seika Kaisha Recombinant beta-fructofuranosidase, its gene and preparation
JP2002515253A (ja) * 1998-05-20 2002-05-28 ノボザイムス バイオテック，インコーポレイティド トリコテセン−欠失糸状菌変異体細胞において異種ポリベプチドを生成するための方法
US6383781B1 (en) * 1998-12-23 2002-05-07 Novozymes A/S Methods for producing polypeptides in aspergillus mutant cells

Also Published As

Publication number Publication date
CN1500882A (zh) 2004-06-02
CN1309711A (zh) 2001-08-22
WO1999060137A2 (fr) 1999-11-25
US20080213901A1 (en) 2008-09-04
US7332341B2 (en) 2008-02-19
WO1999060137A3 (fr) 2000-05-18
EP1078081A2 (fr) 2001-02-28
CN1231579C (zh) 2005-12-14
EP1078081B1 (fr) 2008-05-14
ATE395424T1 (de) 2008-05-15
US6180366B1 (en) 2001-01-30
US20050181480A1 (en) 2005-08-18
AU4195099A (en) 1999-12-06
JP2009291207A (ja) 2009-12-17
CN1190495C (zh) 2005-02-23
JP2002515253A (ja) 2002-05-28
DE69938722D1 (de) 2008-06-26

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