DK0988378T4 - En fremgangsmåde til in vitro molekylær udvikling af proteinfunktion - Google Patents
En fremgangsmåde til in vitro molekylær udvikling af proteinfunktionInfo
- Publication number
- DK0988378T4 DK0988378T4 DK98901380T DK98901380T DK0988378T4 DK 0988378 T4 DK0988378 T4 DK 0988378T4 DK 98901380 T DK98901380 T DK 98901380T DK 98901380 T DK98901380 T DK 98901380T DK 0988378 T4 DK0988378 T4 DK 0988378T4
- Authority
- DK
- Denmark
- Prior art keywords
- protein function
- desired characteristics
- variant
- protein
- scaffold
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Computational Biology (AREA)
- Ecology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9701425.2A GB9701425D0 (en) | 1997-01-24 | 1997-01-24 | A method for in vitro molecular evolution of protein function |
PCT/GB1998/000219 WO1998032845A1 (en) | 1997-01-24 | 1998-01-26 | A method for in vitro molecular evolution of protein function |
Publications (2)
Publication Number | Publication Date |
---|---|
DK0988378T3 DK0988378T3 (da) | 2004-01-26 |
DK0988378T4 true DK0988378T4 (da) | 2009-12-14 |
Family
ID=10806508
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK98901380T DK0988378T4 (da) | 1997-01-24 | 1998-01-26 | En fremgangsmåde til in vitro molekylær udvikling af proteinfunktion |
Country Status (12)
Country | Link |
---|---|
US (4) | US6989250B2 (es) |
EP (2) | EP0988378B9 (es) |
JP (1) | JP3703499B2 (es) |
AT (1) | ATE255163T1 (es) |
AU (1) | AU745615B2 (es) |
CA (1) | CA2278585C (es) |
DE (1) | DE69820054T3 (es) |
DK (1) | DK0988378T4 (es) |
ES (1) | ES2212260T5 (es) |
GB (1) | GB9701425D0 (es) |
PT (1) | PT988378E (es) |
WO (1) | WO1998032845A1 (es) |
Families Citing this family (127)
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1997
- 1997-01-24 GB GBGB9701425.2A patent/GB9701425D0/en active Pending
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1998
- 1998-01-26 EP EP98901380A patent/EP0988378B9/en not_active Expired - Lifetime
- 1998-01-26 EP EP03076606A patent/EP1352959A1/en not_active Withdrawn
- 1998-01-26 DE DE69820054T patent/DE69820054T3/de not_active Expired - Lifetime
- 1998-01-26 DK DK98901380T patent/DK0988378T4/da active
- 1998-01-26 ES ES98901380T patent/ES2212260T5/es not_active Expired - Lifetime
- 1998-01-26 CA CA2278585A patent/CA2278585C/en not_active Expired - Lifetime
- 1998-01-26 JP JP53174298A patent/JP3703499B2/ja not_active Expired - Lifetime
- 1998-01-26 AT AT98901380T patent/ATE255163T1/de active
- 1998-01-26 WO PCT/GB1998/000219 patent/WO1998032845A1/en active IP Right Grant
- 1998-01-26 PT PT98901380T patent/PT988378E/pt unknown
- 1998-01-26 AU AU57718/98A patent/AU745615B2/en not_active Expired
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2002
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2005
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2007
- 2007-04-13 US US11/735,213 patent/US20080108507A1/en not_active Abandoned
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2011
- 2011-05-18 US US13/110,412 patent/US20110287486A1/en not_active Abandoned
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DE69820054T2 (de) | 2004-09-09 |
US6989250B2 (en) | 2006-01-24 |
CA2278585A1 (en) | 1998-07-30 |
EP0988378B1 (en) | 2003-11-26 |
CA2278585C (en) | 2011-09-27 |
JP3703499B2 (ja) | 2005-10-05 |
GB9701425D0 (en) | 1997-03-12 |
ES2212260T5 (es) | 2010-04-05 |
US7432083B2 (en) | 2008-10-07 |
US20030077613A1 (en) | 2003-04-24 |
PT988378E (pt) | 2004-04-30 |
EP0988378B9 (en) | 2010-08-04 |
US20050266451A1 (en) | 2005-12-01 |
US20110287486A1 (en) | 2011-11-24 |
JP2001508660A (ja) | 2001-07-03 |
ES2212260T3 (es) | 2004-07-16 |
EP0988378A1 (en) | 2000-03-29 |
EP1352959A1 (en) | 2003-10-15 |
US20080108507A1 (en) | 2008-05-08 |
AU5771898A (en) | 1998-08-18 |
WO1998032845A1 (en) | 1998-07-30 |
DK0988378T3 (da) | 2004-01-26 |
DE69820054T3 (de) | 2010-05-06 |
ATE255163T1 (de) | 2003-12-15 |
EP0988378B2 (en) | 2009-11-18 |
DE69820054D1 (de) | 2004-01-08 |
AU745615B2 (en) | 2002-03-28 |
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