DK0880536T4 - Fremgangsmåde til fremstilling af renset nukleinsyre og anvendelse deraf - Google Patents

Fremgangsmåde til fremstilling af renset nukleinsyre og anvendelse deraf Download PDF

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Publication number
DK0880536T4
DK0880536T4 DK97901080.8T DK97901080T DK0880536T4 DK 0880536 T4 DK0880536 T4 DK 0880536T4 DK 97901080 T DK97901080 T DK 97901080T DK 0880536 T4 DK0880536 T4 DK 0880536T4
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DK
Denmark
Prior art keywords
nucleic acid
dna
plasmid dna
buffer
mmol
Prior art date
Application number
DK97901080.8T
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English (en)
Other versions
DK0880536T3 (da
Inventor
Wolfgang Kuhne
Original Assignee
Roche Diagnostics Gmbh
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8222465&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK0880536(T4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Application granted granted Critical
Publication of DK0880536T3 publication Critical patent/DK0880536T3/da
Publication of DK0880536T4 publication Critical patent/DK0880536T4/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091—Purification or manufacturing processes for gene therapy compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/16—Central respiratory analeptics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Manufacturing & Machinery (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pulmonology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK97901080.8T 1996-02-06 1997-01-24 Fremgangsmåde til fremstilling af renset nukleinsyre og anvendelse deraf DK0880536T4 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP96101628 1996-02-06
PCT/EP1997/000321 WO1997029113A2 (de) 1996-02-06 1997-01-24 Verfahren zur herstellung von gereinigter nukleinsäure und deren verwendung

Publications (2)

Publication Number Publication Date
DK0880536T3 DK0880536T3 (da) 2003-08-11
DK0880536T4 true DK0880536T4 (da) 2015-09-07

Family

ID=8222465

Family Applications (1)

Application Number Title Priority Date Filing Date
DK97901080.8T DK0880536T4 (da) 1996-02-06 1997-01-24 Fremgangsmåde til fremstilling af renset nukleinsyre og anvendelse deraf

Country Status (12)

Country Link
US (8) US6750333B1 (da)
EP (1) EP0880536B2 (da)
JP (1) JP3442788B2 (da)
KR (1) KR100318808B1 (da)
CN (1) CN1121408C (da)
AT (1) ATE238321T1 (da)
AU (1) AU1444997A (da)
CA (1) CA2245576C (da)
DE (1) DE59709915D1 (da)
DK (1) DK0880536T4 (da)
ES (1) ES2195112T5 (da)
WO (1) WO1997029113A2 (da)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2746412B1 (fr) 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
DE19859703B4 (de) 1998-12-23 2009-10-29 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens
DE19903507A1 (de) 1999-01-29 2000-08-10 Roche Diagnostics Gmbh Verfahren zur Herstellung endotoxinfreier oder an Endotoxin abgereicherter Nukleinsäuren und deren Verwendung
EP1125943B1 (de) * 2000-02-16 2004-10-06 Macherey, Nagel GmbH & Co. Handelsgesellschaft Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens
KR100356738B1 (ko) * 2000-09-07 2002-10-18 주식회사 삼양제넥스 염기성 단백질로부터 엔도톡신을 제거하는 방법
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
GB0217963D0 (en) * 2002-08-02 2002-09-11 Cyclops Genome Sciences Ltd Purification of nucleic acids
DE10238630A1 (de) * 2002-08-19 2004-03-04 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Isolierung biologischer Makromoleküle sowie Vorrichtung zur Durchführung dieses Verfahrens
JP2005538717A (ja) * 2002-09-13 2005-12-22 ヴァレンティス，インコーポレイテッド 核酸のプレパラティブスケールの精製のための装置および方法
US20080154029A1 (en) * 2004-04-02 2008-06-26 Nextec Gmbh Process For Manufacturing a Composite Sorbent Material For Chromatographical Separation of Biopolymers
DK2027272T3 (da) * 2006-05-24 2016-03-14 Scarab Genomics Llc Plasmid-dna-præparater og fremgangsmåder til fremstilling af samme
WO2009092014A1 (en) 2008-01-18 2009-07-23 Gagnon Peter S Enhanced purification of antibodies and antibody fragments by apatite chromatography
SG2014015010A (en) * 2008-04-30 2014-05-29 Gradalis Inc Highly pure plasmid dna preparations and processes for preparing the same
PT2435569T (pt) 2009-05-26 2017-02-03 Eurogentec Sa Método e dispositivo de produção e/ou purificação de polinucleótidos e produtos assim obtidos
WO2011031397A1 (en) 2009-08-06 2011-03-17 Genentech, Inc. Method to improve virus removal in protein purification
CN108148831A (zh) * 2018-01-15 2018-06-12 南京驯鹿医疗技术有限公司 一种无内毒素质粒大量制备工艺

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
JPH01135792A (ja) 1987-11-20 1989-05-29 Mitsui Toatsu Chem Inc 核酸の分離・精製方法
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
DE59505786D1 (de) * 1994-02-07 1999-06-02 Qiagen Gmbh Verfahren zur abreicherung oder entfernung von endotoxinen
US5990301A (en) * 1994-02-07 1999-11-23 Qiagen Gmbh Process for the separation and purification of nucleic acids from biological sources
JPH10503086A (ja) 1994-07-15 1998-03-24 メルク エンド カンパニー インコーポレーテッド 大規模プラスミド精製方法
US5576196A (en) 1995-01-13 1996-11-19 Vical Incorporated Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
US6759394B2 (en) * 2001-07-26 2004-07-06 Board Of Supervisors Of La. State Un. & Agricultural And Mechanical College Cancer gene therapy based on translational control of a suicide gene

Also Published As

Publication number Publication date
US20100074946A1 (en) 2010-03-25
ES2195112T5 (es) 2015-10-15
WO1997029113A3 (de) 1997-10-02
US20060165660A1 (en) 2006-07-27
AU1444997A (en) 1997-08-28
ES2195112T3 (es) 2003-12-01
US20130004562A1 (en) 2013-01-03
US6750333B1 (en) 2004-06-15
CN1210541A (zh) 1999-03-10
US20140287026A1 (en) 2014-09-25
JP3442788B2 (ja) 2003-09-02
CA2245576C (en) 2004-03-23
DE59709915D1 (de) 2003-05-28
CN1121408C (zh) 2003-09-17
US20130315985A1 (en) 2013-11-28
EP0880536B1 (de) 2003-04-23
US20040209835A1 (en) 2004-10-21
US9421279B2 (en) 2016-08-23
ATE238321T1 (de) 2003-05-15
US20110293708A1 (en) 2011-12-01
EP0880536A2 (de) 1998-12-02
KR19990082308A (ko) 1999-11-25
WO1997029113A2 (de) 1997-08-14
CA2245576A1 (en) 1997-08-14
JPH11509554A (ja) 1999-08-24
KR100318808B1 (ko) 2004-05-20
EP0880536B2 (de) 2015-08-05
DK0880536T3 (da) 2003-08-11

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