DK0880536T3 - Fremgangsmåde til fremstilling af renset nukleinsyre og anvendelse deraf - Google Patents

Fremgangsmåde til fremstilling af renset nukleinsyre og anvendelse deraf

Info

Publication number
DK0880536T3
DK0880536T3 DK97901080T DK97901080T DK0880536T3 DK 0880536 T3 DK0880536 T3 DK 0880536T3 DK 97901080 T DK97901080 T DK 97901080T DK 97901080 T DK97901080 T DK 97901080T DK 0880536 T3 DK0880536 T3 DK 0880536T3
Authority
DK
Denmark
Prior art keywords
preparation
nucleic acid
purified nucleic
protein
content
Prior art date
Application number
DK97901080T
Other languages
English (en)
Other versions
DK0880536T4 (da
Inventor
Wolfgang Kuhne
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8222465&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK0880536(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of DK0880536T3 publication Critical patent/DK0880536T3/da
Application granted granted Critical
Publication of DK0880536T4 publication Critical patent/DK0880536T4/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091—Purification or manufacturing processes for gene therapy compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/16—Central respiratory analeptics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Epidemiology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Manufacturing & Machinery (AREA)
  • Pulmonology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DK97901080.8T 1996-02-06 1997-01-24 Fremgangsmåde til fremstilling af renset nukleinsyre og anvendelse deraf DK0880536T4 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP96101628 1996-02-06
PCT/EP1997/000321 WO1997029113A2 (de) 1996-02-06 1997-01-24 Verfahren zur herstellung von gereinigter nukleinsäure und deren verwendung

Publications (2)

Publication Number Publication Date
DK0880536T3 true DK0880536T3 (da) 2003-08-11
DK0880536T4 DK0880536T4 (da) 2015-09-07

Family

ID=8222465

Family Applications (1)

Application Number Title Priority Date Filing Date
DK97901080.8T DK0880536T4 (da) 1996-02-06 1997-01-24 Fremgangsmåde til fremstilling af renset nukleinsyre og anvendelse deraf

Country Status (12)

Country Link
US (8) US6750333B1 (da)
EP (1) EP0880536B2 (da)
JP (1) JP3442788B2 (da)
KR (1) KR100318808B1 (da)
CN (1) CN1121408C (da)
AT (1) ATE238321T1 (da)
AU (1) AU1444997A (da)
CA (1) CA2245576C (da)
DE (1) DE59709915D1 (da)
DK (1) DK0880536T4 (da)
ES (1) ES2195112T5 (da)
WO (1) WO1997029113A2 (da)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2746412B1 (fr) 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
DE19859703B4 (de) 1998-12-23 2009-10-29 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens
DE19903507A1 (de) 1999-01-29 2000-08-10 Roche Diagnostics Gmbh Verfahren zur Herstellung endotoxinfreier oder an Endotoxin abgereicherter Nukleinsäuren und deren Verwendung
EP1125943B1 (de) * 2000-02-16 2004-10-06 Macherey, Nagel GmbH & Co. Handelsgesellschaft Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens
KR100356738B1 (ko) * 2000-09-07 2002-10-18 주식회사 삼양제넥스 염기성 단백질로부터 엔도톡신을 제거하는 방법
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
GB0217963D0 (en) * 2002-08-02 2002-09-11 Cyclops Genome Sciences Ltd Purification of nucleic acids
DE10238630A1 (de) * 2002-08-19 2004-03-04 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Isolierung biologischer Makromoleküle sowie Vorrichtung zur Durchführung dieses Verfahrens
CA2498518A1 (en) * 2002-09-13 2004-03-25 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
ATE547442T1 (de) 2004-04-02 2012-03-15 Nexttec Gmbh Verfahren zur herstellung eines verbundsorptionsmaterials für die chromatographische trennung von biopolymeren
EP2027272B1 (en) * 2006-05-24 2016-01-06 Scarab Genomics, LLC Plasmid dna preparations and methods for producing same
EP2238154B1 (en) 2008-01-18 2015-09-16 Bio-Rad Laboratories, Inc. Enhanced purification of phosphorylated and non-phosphorylated biomolecules by apatite chromatography
EP2283131B1 (en) * 2008-04-30 2016-08-10 Strike Bio, Inc. Highly pure plasmid dna preparations and processes for preparing the same
WO2010136503A1 (en) 2009-05-26 2010-12-02 Eurogentec S.A. Method and device for producing and/or purifying polynucleotides and products obtainable thereof
BR112012002574A2 (pt) * 2009-08-06 2020-11-03 Genentech, Inc. método para aprimorar a remoção de vírus na purificação de proteínas
CN108148831A (zh) * 2018-01-15 2018-06-12 南京驯鹿医疗技术有限公司 一种无内毒素质粒大量制备工艺

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
JPH01135792A (ja) 1987-11-20 1989-05-29 Mitsui Toatsu Chem Inc 核酸の分離・精製方法
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
US5990301A (en) * 1994-02-07 1999-11-23 Qiagen Gmbh Process for the separation and purification of nucleic acids from biological sources
US5747663A (en) * 1994-02-07 1998-05-05 Qiagen Gmbh Process for the depletion or removal of endotoxins
ES2227557T3 (es) 1994-07-15 2005-04-01 MERCK & CO., INC. Procedimiento para la purificacion a gran escala de plasmidos.
US5576196A (en) 1995-01-13 1996-11-19 Vical Incorporated Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
US6759394B2 (en) * 2001-07-26 2004-07-06 Board Of Supervisors Of La. State Un. & Agricultural And Mechanical College Cancer gene therapy based on translational control of a suicide gene

Also Published As

Publication number Publication date
WO1997029113A2 (de) 1997-08-14
US20130004562A1 (en) 2013-01-03
CN1121408C (zh) 2003-09-17
AU1444997A (en) 1997-08-28
DE59709915D1 (de) 2003-05-28
US9421279B2 (en) 2016-08-23
US20130315985A1 (en) 2013-11-28
CN1210541A (zh) 1999-03-10
US20040209835A1 (en) 2004-10-21
EP0880536B2 (de) 2015-08-05
ATE238321T1 (de) 2003-05-15
JPH11509554A (ja) 1999-08-24
ES2195112T5 (es) 2015-10-15
CA2245576C (en) 2004-03-23
EP0880536B1 (de) 2003-04-23
US6750333B1 (en) 2004-06-15
KR100318808B1 (ko) 2004-05-20
US20100074946A1 (en) 2010-03-25
US20140287026A1 (en) 2014-09-25
ES2195112T3 (es) 2003-12-01
CA2245576A1 (en) 1997-08-14
KR19990082308A (ko) 1999-11-25
WO1997029113A3 (de) 1997-10-02
US20060165660A1 (en) 2006-07-27
DK0880536T4 (da) 2015-09-07
US20110293708A1 (en) 2011-12-01
JP3442788B2 (ja) 2003-09-02
EP0880536A2 (de) 1998-12-02

Similar Documents

Publication Publication Date Title
DK0880536T3 (da) Fremgangsmåde til fremstilling af renset nukleinsyre og anvendelse deraf
Lee et al. Effects of a hexameric deoxyriboguanosine run conjugation into CpG oligodeoxynucleotides on their immunostimulatory potentials
NO934235L (no) Anvendelse av nukleinsyreanaloger for diagnostiske og analytiske fremgangsmaater
IT1206311B (it) Agente terapeutico a base di oligonucleotide e metodi per laproduzione dello stesso.
ATE258434T1 (de) Sich selbst zusammensetzendes polynukleotid- abgabesystem, das dendrimer-polykationen enthält
ATE225369T1 (de) Doppelsträngige peptidenukleinsäuren
DE19975064I1 (de) Tumornekrosefaktor, Verfahren zu seiner Herstellung, denselben enthaltende Zusammensetzungen, daf}r kodierende DNS und diese DNS verwendendes Testfahren.
DE3781005D1 (de) Adsorptionsmittel fuer chromatographie, methode zur herstellung desselben und saeulensystem fuer chromatographie.
DE60113805D1 (de) Intratumorale verabreichung nackter il-12 codierender nukleinsäuremoleküle
TW261615B (da)
AU7247891A (en) Purified myeloblastin, nucleic acid molecule encoding same, and uses thereof
LTIP1753A (en) Dna sequences coding for proteins, biotechnological methods for the preparation of said proteins and pharmaceutical compositions containing said proteins
ES2194024T3 (es) Una proteina de de superficie celular, denominada proteina rib, que confiere inmunidad a muchas cepas de estreptococos del grupo b; procedimiento para purificar la proteina, kit de reactivos y composicion farmaceutica.
WO1998006841A3 (en) Two human nsp-like proteins
ATE342732T1 (de) Anti-allergische pharmazeutische zusammensetzung
AR033340A1 (es) Factor activador de la citoquina altamente purificado y metodos de uso
ES2170747T3 (es) Analogos superiores de trombomodulina para uso farmaceutico.
IT8919696A0 (it) Sistema di profilati, per la realizzazione di serramenti in genere.
DK0643719T3 (da) Nukleinsyresekvenser, som koder for eller er komplementære til nukleinsyresekvenser, som koder for interleukin 9-receptor
BR0007528A (pt) Métodos para clarificação de soluções de açúcar
EP1246678A4 (en) VEHICLES FOR SEPARATING PHYSIOLOGICAL FLUIDS HAVING INCREASED RESISTANCE TO INTERNAL MIGRATION OF THERAPEUTIC AGENTS
Shibata et al. Low molecular weight nuclear RNA. The 3′-terminal sequence of the U2 RNA
Thang et al. Interaction of interferon with tRNA
Patel et al. RNA associated with nonhistone chromosomal proteins of dog liver
BR9909576A (pt) Genes de receptor de transferrina de moraxella