DK0849277T3 - Fremgangsmåde til udvinding af insulin ved höjtryksvæskekromatografi - Google Patents

Fremgangsmåde til udvinding af insulin ved höjtryksvæskekromatografi

Info

Publication number
DK0849277T3
DK0849277T3 DK97121847T DK97121847T DK0849277T3 DK 0849277 T3 DK0849277 T3 DK 0849277T3 DK 97121847 T DK97121847 T DK 97121847T DK 97121847 T DK97121847 T DK 97121847T DK 0849277 T3 DK0849277 T3 DK 0849277T3
Authority
DK
Denmark
Prior art keywords
liquid chromatography
pressure liquid
insulin
extracting insulin
extracting
Prior art date
Application number
DK97121847T
Other languages
English (en)
Inventor
Rainer Dr Obermeier
Juergen Ludwig
Walter Dl Sabel
Original Assignee
Aventis Pharma Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Gmbh filed Critical Aventis Pharma Gmbh
Application granted granted Critical
Publication of DK0849277T3 publication Critical patent/DK0849277T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK97121847T 1996-12-18 1997-12-11 Fremgangsmåde til udvinding af insulin ved höjtryksvæskekromatografi DK0849277T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19652713A DE19652713C2 (de) 1996-12-18 1996-12-18 Verfahren zur Reinigung von Insulin und Insulinderivaten durch Chromatographie an stark saurem Kationenaustauscher

Publications (1)

Publication Number Publication Date
DK0849277T3 true DK0849277T3 (da) 2005-04-11

Family

ID=7815158

Family Applications (1)

Application Number Title Priority Date Filing Date
DK97121847T DK0849277T3 (da) 1996-12-18 1997-12-11 Fremgangsmåde til udvinding af insulin ved höjtryksvæskekromatografi

Country Status (11)

Country Link
US (1) US5977297A (da)
EP (1) EP0849277B1 (da)
JP (1) JP4975895B2 (da)
KR (1) KR19980064213A (da)
AT (1) ATE284899T1 (da)
AU (1) AU724931B2 (da)
CA (1) CA2225178C (da)
DE (2) DE19652713C2 (da)
DK (1) DK0849277T3 (da)
ES (1) ES2233990T3 (da)
PT (1) PT849277E (da)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19838097A1 (de) * 1998-08-24 2000-03-02 Hoechst Marion Roussel De Gmbh Verfahren zur chromatographischen Reinigung von Insulinen
US6451987B1 (en) * 1999-03-15 2002-09-17 Novo Nordisk A/S Ion exchange chromatography of proteins and peptides
US6444788B1 (en) 1999-03-15 2002-09-03 Novo Nordisk A/S Ion exchange chromatography of GLP-1, analogs and derivatives thereof
US7608583B2 (en) * 2002-12-05 2009-10-27 Wockhardt Limited Process for the extraction and isolation of insulin from recombinant sources
CN1771080B (zh) * 2003-04-08 2010-12-15 诺沃挪第克公司 包括至少一个色谱处理步骤的生产治疗用多肽或其前体的方法
JP5274771B2 (ja) * 2003-04-08 2013-08-28 ノヴォ ノルディスク アー／エス クロマトグラフィー固定相の再生
RU2006103860A (ru) 2003-08-21 2006-07-27 Ново Нордиск А/С (DK) Способ разделения полипептидов, содержащих рацемическую аминокислоту
US20080108787A1 (en) * 2004-05-24 2008-05-08 Maharaj K Sahib Purification of Insulin-Like Material by Reverse Phase Chromatography
US7241586B2 (en) * 2005-02-17 2007-07-10 Medtronic Minimed, Inc. Polypeptide formulations and methods for making, using and characterizing them
US8343914B2 (en) * 2006-01-06 2013-01-01 Case Western Reserve University Fibrillation resistant proteins
ES2554773T3 (es) 2006-10-04 2015-12-23 Case Western Reserve University Insulina y análogos de la insulina resistentes a la fibrilación
US8993516B2 (en) * 2008-04-14 2015-03-31 Case Western Reserve University Meal-time insulin analogues of enhanced stability
RU2010147076A (ru) * 2008-04-22 2012-05-27 Кейз Вестерн Ризев Юнивесити (Us) Аналоги инсулина специфичные к изоформам
PL219335B1 (pl) 2008-07-04 2015-04-30 Inst Biotechnologii I Antybiotyków Pochodna insuliny lub jej farmaceutycznie dopuszczalna sól, jej zastosowanie oraz zawierająca ją kompozycja farmaceutyczna
US9200053B2 (en) 2008-07-31 2015-12-01 Case Western Reserve University Insulin analogues containing penta-fluoro-Phenylalanine at position B24
KR20120129875A (ko) 2008-07-31 2012-11-28 케이스 웨스턴 리저브 유니버시티 염소화 아미노산을 갖는 인슐린 유사체
US8399407B2 (en) * 2009-09-17 2013-03-19 Case Western Reserve University Non-standard insulin analogues
MX2012006568A (es) 2009-12-11 2012-12-17 Univ Case Western Reserve Analogos de insulina con aminoacidos clorados.
CN103827136B (zh) * 2011-09-30 2016-07-06 通用电气健康护理生物科学股份公司 纯化裂解的胰岛素原的方法
EP2931301B2 (en) * 2012-12-17 2021-09-15 Merck Sharp & Dohme Corp. Process for purifying insulin and analogues thereof
WO2015138548A1 (en) 2014-03-14 2015-09-17 Merck Sharp & Dohme Corp. Purifying insulin using cation exchange and reverse phase chromatography in the presence of an organic modifier and elevated temperature
US10155799B2 (en) 2014-07-21 2018-12-18 Merck Sharp & Dohme Corp. Chromatography process for purification of insulin and insulin analogs
JP6829928B2 (ja) 2014-10-06 2021-02-17 ケース ウェスタン リザーブ ユニバーシティＣａｓｅ Ｗｅｓｔｅｒｎ Ｒｅｓｅｒｖｅ Ｕｎｉｖｅｒｓｉｔｙ 二相性単鎖インスリン類似体
US20170121365A1 (en) * 2015-10-28 2017-05-04 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
EP3581578A4 (en) * 2017-02-10 2020-04-22 Mitsubishi Chemical Corporation RELEASE AGENT FOR PURIFYING HUMAN INSULIN AND METHOD FOR PURIFYING HUMAN INSULIN

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3147842A1 (de) * 1981-12-03 1983-06-23 Hoechst Ag, 6230 Frankfurt "verfahren zur trennung von gemischen aus insulin, insulinderivaten und gegebenenfalls verunreinigungen"
DE3726655A1 (de) * 1987-08-11 1989-02-23 Hoechst Ag Verfahren zur isolierung basischer proteine aus proteingemischen, welche solche basischen proteine enthalten
DE3837825A1 (de) * 1988-11-08 1990-05-10 Hoechst Ag Neue insulinderivate, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
DE4028120C2 (de) * 1990-09-05 1996-09-19 Hoechst Ag Verfahren zur Reinigung von Insulin und/oder Insulinderivaten
DK0547544T3 (da) * 1991-12-18 1997-09-08 Hoechst Ag Fremgangsmåde til fremstilling af insulinholdige opløsninger.

Also Published As

Publication number Publication date
EP0849277A3 (de) 2002-06-05
ATE284899T1 (de) 2005-01-15
EP0849277B1 (de) 2004-12-15
DE19652713A1 (de) 1998-06-25
AU4842197A (en) 1998-06-25
PT849277E (pt) 2005-03-31
CA2225178C (en) 2009-06-09
US5977297A (en) 1999-11-02
AU724931B2 (en) 2000-10-05
EP0849277A2 (de) 1998-06-24
DE19652713C2 (de) 2001-11-22
CA2225178A1 (en) 1998-06-18
DE59712121D1 (de) 2005-01-20
KR19980064213A (ko) 1998-10-07
ES2233990T3 (es) 2005-06-16
JPH10182700A (ja) 1998-07-07
JP4975895B2 (ja) 2012-07-11

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