DK0675969T3 - Fremgangsmåde til mangfoldiggørelse af målrettede nucleinsyresegmenter under anvendelse af grupperet polymerase-kædereaktion - Google Patents

Fremgangsmåde til mangfoldiggørelse af målrettede nucleinsyresegmenter under anvendelse af grupperet polymerase-kædereaktion

Info

Publication number
DK0675969T3
DK0675969T3 DK94903404.5T DK94903404T DK0675969T3 DK 0675969 T3 DK0675969 T3 DK 0675969T3 DK 94903404 T DK94903404 T DK 94903404T DK 0675969 T3 DK0675969 T3 DK 0675969T3
Authority
DK
Denmark
Prior art keywords
polymerase chain
chain reaction
grouped
multiplying
nucleic acid
Prior art date
Application number
DK94903404.5T
Other languages
English (en)
Inventor
Mark Anton Jensen
Ron Grosz
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Application granted granted Critical
Publication of DK0675969T3 publication Critical patent/DK0675969T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK94903404.5T 1992-12-09 1993-12-08 Fremgangsmåde til mangfoldiggørelse af målrettede nucleinsyresegmenter under anvendelse af grupperet polymerase-kædereaktion DK0675969T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/990,297 US5340728A (en) 1992-12-09 1992-12-09 Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction

Publications (1)

Publication Number Publication Date
DK0675969T3 true DK0675969T3 (da) 1997-04-28

Family

ID=25535998

Family Applications (1)

Application Number Title Priority Date Filing Date
DK94903404.5T DK0675969T3 (da) 1992-12-09 1993-12-08 Fremgangsmåde til mangfoldiggørelse af målrettede nucleinsyresegmenter under anvendelse af grupperet polymerase-kædereaktion

Country Status (13)

Country Link
US (1) US5340728A (da)
EP (1) EP0675969B1 (da)
JP (1) JPH08504332A (da)
AT (1) ATE148925T1 (da)
AU (1) AU5736194A (da)
CA (1) CA2151036A1 (da)
DE (1) DE69308162T2 (da)
DK (1) DK0675969T3 (da)
ES (1) ES2097633T3 (da)
GR (1) GR3023325T3 (da)
IL (1) IL107935A0 (da)
MX (1) MX9307788A (da)
WO (1) WO1994013832A1 (da)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5817797A (en) * 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
US20020127548A1 (en) * 1992-12-11 2002-09-12 Christine Siedman Methods for detecting mutations associated with hypertrophic cardiomyopathy
US5912121A (en) * 1992-12-11 1999-06-15 Bringham And Women's Hospital Methods for detecting mutations associated with hypertrophic cardiomyopathy
US5556773A (en) * 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5928905A (en) * 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5660981A (en) * 1994-06-06 1997-08-26 E. I. Du Pont De Nemours And Company Selection of diagnostic genetic markers in microorganisms and use of a specific marker for detection of salmonella
US5559013A (en) * 1994-06-22 1996-09-24 Johnson & Johnson Clinical Diagnostics, Inc. Method of amplification using intermediate renaturation step
US5705366A (en) * 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
ATE407207T1 (de) 1994-11-02 2008-09-15 Trophix Pharm Inc Natrium-ionenkanäle spezifisch für das periphäre nervensystem, dna welche diese kodiert, wirkstoff-suche und methoden zu deren herstellung und verwendung
US6110665A (en) * 1995-02-14 2000-08-29 University Of Kentucky Research Foundation Sarcocystis neuronadiagnostic primer and its use in methods of equine protozoal myeloencephalitis diagnosis
GB9509112D0 (en) * 1995-05-04 1995-06-28 Scottish Crop Research Inst Polynucleotide
GB9509410D0 (en) * 1995-05-10 1995-07-05 Imperial College Molecular imaging
US5693467A (en) * 1995-05-19 1997-12-02 The American Type Culture Collection Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers
DE69620596T2 (de) * 1995-05-19 2002-11-21 Abbott Laboratories, Abbott Park Nachweis von nukleinsäuren mit breitem dynamikbereich unter verwendung von aggregat-primer-reihen
CN1151269C (zh) * 1995-06-06 2004-05-26 扬·维吉 用于诊断性dna检测的方法
EP0832280A2 (en) * 1995-06-07 1998-04-01 Abbott Laboratories Probe masking method of reducing background in an amplification reaction
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
EP0785281B1 (en) * 1995-07-28 2004-05-19 Sapporo Breweries Ltd. Genetic variety identifying method in hop
US5747257A (en) * 1996-02-29 1998-05-05 E. I. Du Pont De Nemours And Company Genetic markers and methods for the detection of escherichia coli serotype-0157:H7
US6458530B1 (en) * 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
CA2257109C (en) * 1996-06-04 2009-10-06 University Of Utah Research Foundation Monitoring hybridization during pcr
US5989873A (en) * 1996-09-24 1999-11-23 Vinayagamoorthy; Thuraiayah Method of detecting gene expression and/or of preventing such expression in cells
US5922538A (en) * 1996-11-08 1999-07-13 E.I. Du Pont De Nemours And Company Genetic markers and methods for the detection of Listeria monocytogenes and Listeria spp
IL120339A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
US6103468A (en) * 1997-10-07 2000-08-15 Labatt Brewing Company Limited Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer
US6468743B1 (en) * 1998-05-18 2002-10-22 Conagra Grocery Products Company PCR techniques for detecting microbial contaminants in foodstuffs
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
ES2189631B1 (es) * 2001-03-12 2005-01-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Metodo para la amplificacion de adn mediante semi-nested pcr en un solo paso y su empleo en la identificacion de especies de plasmodium.
JP3979024B2 (ja) * 2001-03-29 2007-09-19 日本製紙株式会社 Ｒａｐｄ法による個体識別方法
MXPA03012075A (es) * 2001-06-22 2005-07-01 Marshfield Clinic Metodos y oligonucleotidos para la deteccion de salmonela sp., e. coli o157:h7 y listeria monocitogenes.
WO2003100019A2 (en) * 2002-05-24 2003-12-04 Invitrogen Corporation Nested pcr employing degradable primers
US8323897B2 (en) 2002-12-04 2012-12-04 Applied Biosystems, Llc Multiplex amplification of polynucleotides
WO2005028629A2 (en) * 2003-09-19 2005-03-31 Applera Corporation Whole genome expression analysis system
US20060024690A1 (en) * 2003-09-19 2006-02-02 Kao H P Normalization of data using controls
NZ546903A (en) * 2003-10-13 2009-06-26 Genaco Biomedical Products Inc Method and kit for primer based amplification of nucleic acids
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
US20060003337A1 (en) * 2004-06-30 2006-01-05 John Brandis Detection of small RNAS
KR100671501B1 (ko) * 2005-02-28 2007-01-19 삼성에버랜드 주식회사 식중독 검출용 프라이머 및 이를 이용한 세균성 식중독 검출방법
US20060240442A1 (en) * 2005-04-20 2006-10-26 Vevea Dirk N Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes
JP2009531464A (ja) 2006-03-29 2009-09-03 メリアル リミテッド 連鎖球菌に対するワクチン
FR2926367B1 (fr) * 2008-01-10 2013-01-04 Novaleads Procede fluorimetrique pour evaluer l'influence d'une condition sur un echantillon biologique et ses applications.
EP2438184B1 (en) * 2009-06-02 2014-06-04 Monoquant PTY LTD A method of nucleic acid amplification
US9388473B2 (en) 2011-11-09 2016-07-12 E I Du Pont De Nemours And Company Sequences and their use for detection of salmonella
US20230366038A1 (en) * 2022-05-16 2023-11-16 Bioo Scientific Corporation Compositions and methods relating to loop mediated isothermal amplification (lamp)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
JPH0638759B2 (ja) * 1988-07-29 1994-05-25 株式会社島津製作所 サルモネラ菌検査に用いるｄｎａプローブ及びそれを用いたサルモネラ菌の検出法
AU636875B2 (en) * 1989-03-17 1993-05-13 Abbott Laboratories Method and device for improved reaction kinetics in nucleic acid hybridizations
DE69003104T2 (de) * 1989-06-12 1994-03-17 Cis Bio International Saclay Verfahren zum nachweis von spezifischen sequenzen von nukleinsäuren und ihre verwendungen.
EP0409159B1 (en) * 1989-07-18 1998-11-25 Shimadzu Corporation Method for testing causative microorganism of food poisoning and reagents therefor
US5126239A (en) * 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
JPH0491790A (ja) * 1990-08-02 1992-03-25 Shionogi & Co Ltd ２段階ｐｃｒ法
GB9107124D0 (en) * 1991-04-05 1991-05-22 Dynal As Chemical process

Also Published As

Publication number Publication date
CA2151036A1 (en) 1994-06-23
DE69308162D1 (de) 1997-03-27
IL107935A0 (en) 1994-04-12
EP0675969A1 (en) 1995-10-11
MX9307788A (es) 1994-07-29
DE69308162T2 (de) 1997-06-26
GR3023325T3 (en) 1997-08-29
JPH08504332A (ja) 1996-05-14
WO1994013832A1 (en) 1994-06-23
ES2097633T3 (es) 1997-04-01
US5340728A (en) 1994-08-23
ATE148925T1 (de) 1997-02-15
EP0675969B1 (en) 1997-02-12
AU5736194A (en) 1994-07-04

Similar Documents

Publication Publication Date Title
DK0675969T3 (da) Fremgangsmåde til mangfoldiggørelse af målrettede nucleinsyresegmenter under anvendelse af grupperet polymerase-kædereaktion
IL131966A0 (en) An in vitro method for construction of a dna library
ATE342356T1 (de) Prostata-spezifisches membranantigen
ATE188743T1 (de) Oligonukleotiden komplementär zu mycobakterieller nukleinsäure
DK0726324T3 (da) Anvendelse af exonuklease og/eller glycosylase som supplementer til antipolymeraseantistof til forøgelse af specification i polymerasekædereaktion
DK0669401T3 (da) Amplificering af lange nukleinsyresekvenser ved PCR
PT87769A (pt) Transcripton-based nucleic acid amplification detection systems
EA200300991A1 (ru) Продукт, содержащий иммобилизованную нуклеиновую кислоту, полученный способом с участием химерного олигонуклеотидного праймера, днк-полимеразы и эндонуклеазы
DK1300466T3 (da) En kaskade nukleinsyre amplifikationsreaktion
DK0401037T3 (da) Fremgangsmåde til styring af kontaminationen af nukleinsyreamplifikationsreaktioner
DK153289A (da) Selektiv forstaerkning af maal-polynucleotidsekvenser
ATE161291T1 (de) Verfahren und reagenzien zum nachweis von hepatitis c
NO20003058D0 (no) Massespektrometriske metoder for sekvensering av nukleinsyrer
NO962062L (no) Fremgangsmåte for polymerasekjede-reaksjons preamplifikasjons sterilisering ved eksonukleaser i nærvær av fosforotioaterte primere
ATE361377T1 (de) Leistungstarke verknüpfung von nukleinsäuresegmenten
DK1026261T3 (da) Forögelse af specificiteten af nukleinsyreamplifikation med bærernukleinsyre
DE69427876D1 (de) Methode zur detektion einer gezielten nukleinsäure
DE69804334D1 (de) Steuerung von nukleinsäure-amplifikationsverfahren einschlie lich der polymerase-kettenreaktion
DE50214183D1 (de) Verfahren zum nachweis von in einer polymerase-kettenreaktion amplifizierten nukleinsäuremolekülen
ATE197481T1 (de) Verfahren und reagenzien zur typisierung von hla- genen der klasse i
HUP0000500A2 (hu) In vitro eljárás DNS-könyvtár készítésére
ES2129365B1 (es) Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno.