DE69941269D1 - Verbesserte verfahren zur einschleusung von nukleinsäuren in ringförmige vektoren - Google Patents
Verbesserte verfahren zur einschleusung von nukleinsäuren in ringförmige vektorenInfo
- Publication number
- DE69941269D1 DE69941269D1 DE69941269T DE69941269T DE69941269D1 DE 69941269 D1 DE69941269 D1 DE 69941269D1 DE 69941269 T DE69941269 T DE 69941269T DE 69941269 T DE69941269 T DE 69941269T DE 69941269 D1 DE69941269 D1 DE 69941269D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- present
- vectors
- circularization
- inserts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 7
- 102000039446 nucleic acids Human genes 0.000 title abstract 7
- 108020004707 nucleic acids Proteins 0.000 title abstract 7
- 239000013598 vector Substances 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 5
- 238000003780 insertion Methods 0.000 abstract 4
- 230000037431 insertion Effects 0.000 abstract 4
- 238000010367 cloning Methods 0.000 abstract 2
- 101710183280 Topoisomerase Proteins 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/213,834 US6825011B1 (en) | 1998-12-17 | 1998-12-17 | Methods for insertion of nucleic acids into circular vectors |
| PCT/US1999/030277 WO2000036088A1 (en) | 1998-12-17 | 1999-12-17 | Improved methods for insertion of nucleic acids into circular vectors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE69941269D1 true DE69941269D1 (de) | 2009-09-24 |
Family
ID=22796685
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69941269T Expired - Fee Related DE69941269D1 (de) | 1998-12-17 | 1999-12-17 | Verbesserte verfahren zur einschleusung von nukleinsäuren in ringförmige vektoren |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6825011B1 (de) |
| EP (1) | EP1141239B8 (de) |
| JP (1) | JP2002532085A (de) |
| AT (1) | ATE439428T1 (de) |
| AU (1) | AU773922B2 (de) |
| CA (1) | CA2355349A1 (de) |
| DE (1) | DE69941269D1 (de) |
| WO (1) | WO2000036088A1 (de) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1504119A4 (de) * | 2000-02-25 | 2005-02-09 | Stratagene California | Verfahren zur ligation von nukleinsäuren und molekularer klonierung |
| US7517688B2 (en) | 2000-02-25 | 2009-04-14 | Stratagene California | Method for ligating nucleic acids and molecular cloning |
| US6312913B1 (en) * | 2000-07-21 | 2001-11-06 | Incyte Genomics, Inc. | Method for isolating and characterizing nucleic acid sequences |
| WO2002008408A2 (en) * | 2000-07-21 | 2002-01-31 | Trustees Of Boston University | Modular vector systems |
| JP5043277B2 (ja) | 2000-08-21 | 2012-10-10 | ライフ テクノロジーズ コーポレーション | 分子クローニング法および使用試薬 |
| WO2003040324A2 (en) * | 2001-11-02 | 2003-05-15 | Athersys, Inc. | Methods for mutating genes in cells and animals using insertional mutagenesis |
| AU2004311882A1 (en) * | 2003-12-29 | 2005-07-21 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
| JP2008505905A (ja) | 2004-07-07 | 2008-02-28 | メルク エンド カムパニー インコーポレーテッド | ピラゾールアミド誘導体、こうした化合物を含有する組成物および使用方法 |
| WO2008141514A1 (en) * | 2007-05-17 | 2008-11-27 | Wondergen Bio-Medicine Technology Co., Ltd. | System substitute pcr |
| US7947446B2 (en) * | 2007-05-29 | 2011-05-24 | Ming-Sheng Lee | High throughput mutation screening methods and kits using a universalized approach—differential sequence fill-in (DSF)-enabled sequential adapter ligation and amplification |
| CN102911961B (zh) * | 2011-08-01 | 2014-07-16 | 尚玉栓 | 一种线性化表达载体构建方法及用于线性化表达载体构建的类载体片段 |
| US9206418B2 (en) | 2011-10-19 | 2015-12-08 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
| CN105861487B (zh) | 2012-01-26 | 2020-05-05 | 纽亘技术公司 | 用于靶向核酸序列富集和高效文库产生的组合物和方法 |
| SG11201408478QA (en) | 2012-06-18 | 2015-02-27 | Nugen Technologies Inc | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| US9822408B2 (en) | 2013-03-15 | 2017-11-21 | Nugen Technologies, Inc. | Sequential sequencing |
| CA2929596C (en) | 2013-11-13 | 2022-07-05 | Nugen Technologies, Inc. | Compositions and methods for identification of a duplicate sequencing read |
| US9745614B2 (en) | 2014-02-28 | 2017-08-29 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| CN108410895A (zh) * | 2018-01-23 | 2018-08-17 | 南华大学 | 一种提高重组dna片段转化大肠杆菌效率的方法 |
| EP3911750A4 (de) * | 2019-01-15 | 2022-09-28 | University of Maryland, Baltimore County | Verfahren zum geordneten zusammenbau von zirkulären und linearen dns-molekülen |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0484440A4 (en) | 1989-07-28 | 1993-01-07 | Us Health | Efficient directional genetic cloning system |
| GB9302798D0 (en) * | 1993-02-12 | 1993-03-31 | Medical Res Council | Improvements in or relating to cloning dna |
| US5633149A (en) * | 1994-12-07 | 1997-05-27 | Incyte Pharmaceuticals, Inc. | Polynucleotide encoding novel chemokine expressed in inflamed adenoid |
| IL120337A0 (en) | 1997-02-27 | 1997-06-10 | Gesher Israel Advanced Biotecs | Method for joining DNA fragments |
| IL120338A0 (en) | 1997-02-27 | 1997-06-10 | Gesher Israel Advanced Biotecs | Single step DNA fragments assembly |
| IL120339A0 (en) | 1997-02-27 | 1997-06-10 | Gesher Israel Advanced Biotecs | Improved DNA assembly method |
-
1998
- 1998-12-17 US US09/213,834 patent/US6825011B1/en not_active Expired - Fee Related
-
1999
- 1999-12-17 WO PCT/US1999/030277 patent/WO2000036088A1/en active IP Right Grant
- 1999-12-17 AU AU23707/00A patent/AU773922B2/en not_active Ceased
- 1999-12-17 JP JP2000588337A patent/JP2002532085A/ja active Pending
- 1999-12-17 CA CA002355349A patent/CA2355349A1/en not_active Abandoned
- 1999-12-17 EP EP99967431A patent/EP1141239B8/de not_active Expired - Lifetime
- 1999-12-17 AT AT99967431T patent/ATE439428T1/de not_active IP Right Cessation
- 1999-12-17 DE DE69941269T patent/DE69941269D1/de not_active Expired - Fee Related
-
2004
- 2004-09-15 US US10/942,251 patent/US20050069524A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU2370700A (en) | 2000-07-03 |
| CA2355349A1 (en) | 2000-06-22 |
| JP2002532085A (ja) | 2002-10-02 |
| EP1141239B8 (de) | 2009-10-07 |
| US20050069524A1 (en) | 2005-03-31 |
| AU773922B2 (en) | 2004-06-10 |
| ATE439428T1 (de) | 2009-08-15 |
| EP1141239A4 (de) | 2005-04-27 |
| WO2000036088A1 (en) | 2000-06-22 |
| EP1141239A1 (de) | 2001-10-10 |
| EP1141239B1 (de) | 2009-08-12 |
| US6825011B1 (en) | 2004-11-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8327 | Change in the person/name/address of the patent owner |
Owner name: ROMANTCHIKOV, YURI, HAGERSTOWN, MD., US |
|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |