DE69918216D1 - Verfahren zur Herstellung transgener Gurken mit hohem Superoxid-dismutasegehalt - Google Patents

Verfahren zur Herstellung transgener Gurken mit hohem Superoxid-dismutasegehalt

Info

Publication number
DE69918216D1
DE69918216D1 DE69918216T DE69918216T DE69918216D1 DE 69918216 D1 DE69918216 D1 DE 69918216D1 DE 69918216 T DE69918216 T DE 69918216T DE 69918216 T DE69918216 T DE 69918216T DE 69918216 D1 DE69918216 D1 DE 69918216D1
Authority
DE
Germany
Prior art keywords
cucumbers
transgenic
production
superoxide dismutase
dismutase content
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69918216T
Other languages
English (en)
Inventor
Jae-Whune Kim
Haeng-Soon Lee
Suk Yoon Kwon
Sang Soo Kwak
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Institute of Science and Technology KIST
Original Assignee
Korea Institute of Science and Technology KIST
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1019980013205A external-priority patent/KR100270910B1/ko
Priority claimed from KR1019980033947A external-priority patent/KR100311609B1/ko
Priority claimed from KR1019990011848A external-priority patent/KR100360339B1/ko
Application filed by Korea Institute of Science and Technology KIST filed Critical Korea Institute of Science and Technology KIST
Application granted granted Critical
Publication of DE69918216D1 publication Critical patent/DE69918216D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8235—Fruit-specific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Pregnancy & Childbirth (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Reproductive Health (AREA)
  • Dermatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE69918216T 1998-04-14 1999-04-14 Verfahren zur Herstellung transgener Gurken mit hohem Superoxid-dismutasegehalt Expired - Lifetime DE69918216D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1019980013205A KR100270910B1 (ko) 1998-04-14 1998-04-14 수퍼옥사이드 디스뮤타제를 대량생산하는 형질전환된 식물체, 그의 제조방법 및 용도
KR1019980033947A KR100311609B1 (ko) 1998-08-21 1998-08-21 종자식물 유식물체의 하배축 절단면에서 부정아를 고빈도로 유도하는 방법 및 이로부터 재분화 식물체와 형질전환 식물체를 제조하는 방법
KR1019990011848A KR100360339B1 (ko) 1999-04-06 1999-04-06 수퍼옥사이드 디스뮤타제를 대량 생산하는 형질전환된 오이의 제조방법

Publications (1)

Publication Number Publication Date
DE69918216D1 true DE69918216D1 (de) 2004-07-29

Family

ID=27349712

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69918216T Expired - Lifetime DE69918216D1 (de) 1998-04-14 1999-04-14 Verfahren zur Herstellung transgener Gurken mit hohem Superoxid-dismutasegehalt

Country Status (4)

Country Link
US (1) US6084152A (de)
EP (1) EP0952224B1 (de)
JP (1) JP3307604B2 (de)
DE (1) DE69918216D1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6544399B1 (en) 1999-01-11 2003-04-08 Applied Materials, Inc. Electrodeposition chemistry for filling apertures with reflective metal
KR100447920B1 (ko) * 2002-01-09 2004-09-08 (주)넥스젠 오이 형질전환체의 제조방법 및 형질전환 오이
NL1022270C2 (nl) 2002-12-24 2004-06-25 Nunhems Zaden Bv Volledige echte meeldauwresistentie en volledige vrijheid van necrose in komkommer, Cucumis sativus L.
KR101696504B1 (ko) * 2009-09-02 2017-01-13 액세스 비지니스 그룹 인터내셔날 엘엘씨 피부 복구용 조성물 및 방법
CN102870674B (zh) * 2012-09-05 2013-12-25 广州白云华南生物科技有限公司 一种红葱组培快速繁殖方法
US8847022B2 (en) 2012-10-19 2014-09-30 Nunhems B.V. Cucumber variety NUN 55507 CUP
US9234207B2 (en) 2012-11-05 2016-01-12 Nunhems B.V. Cucumber variety NUN 5545 CUP
FR2999386B1 (fr) * 2012-12-17 2015-04-03 Bionov Composition pour stimuler la vitalite des plantes
US10201145B2 (en) 2015-03-10 2019-02-12 Nunhems B.V. Cucumber variety NUN 52007 CUP
US10172315B2 (en) 2015-03-10 2019-01-08 Nunhems B.V. Cucumber variety NUN 55513 CUP
US10172316B2 (en) 2015-03-10 2019-01-08 Nunhems B.V. Cucumber variety NUN 53016 CUP
US10098311B2 (en) 2015-09-15 2018-10-16 Nunhems B.V. Cucumber variety NUN 43003 CUL
US10064352B2 (en) 2015-10-07 2018-09-04 Nunhems B.V. Cucumber variety NUN 53019 CUP
US10492409B2 (en) 2016-12-01 2019-12-03 Nunhems B.V. Cucumber variety NUN 55516 CUP
US10264753B2 (en) 2016-12-07 2019-04-23 Nunhems B.V. Cucumber variety NUN 53025 CUP
US10271504B2 (en) 2016-12-07 2019-04-30 Nunhems B.V. Cucumber variety NUN 53031 CUP
US10448592B2 (en) 2017-03-21 2019-10-22 Nunhems B.V. Cucumber variety NUN 52010 CUP
US10701888B2 (en) 2017-06-01 2020-07-07 Nunhems B.V. Tomato variety NUN 09196 TOF
US10716272B2 (en) 2017-08-04 2020-07-21 Nunhems B.V. Cucumber variety NUN 51024 CUP
WO2020048836A1 (en) * 2018-09-03 2020-03-12 Arterra Bioscience S.R.L. Industrial applications of plant cell extracts comprising sod enzymes of extremophilic micro-organisms
CN110583411A (zh) * 2019-10-30 2019-12-20 怀化市九天界生态农业科技有限公司 一种黄精育苗方法
US11690340B2 (en) 2020-02-28 2023-07-04 Nunhems B.V. Cucumber variety NUN 53050 CUP
CN111909953B (zh) * 2020-06-05 2023-07-07 东北林业大学 用于桑黄遗传表达的重组载体及构建方法和遗传转化方法
US11445673B2 (en) 2020-07-02 2022-09-20 Nunhems B.V. Cucumber variety NUN 83067 CUL
CN111926033A (zh) * 2020-07-31 2020-11-13 上海交通大学 一种基于草铵膦为筛选标记的甜瓜遗传转化方法
CA3124484A1 (en) 2021-07-12 2023-01-12 Nunhems B.V. Cucumber variety nun 32361 cus
CN113462663A (zh) * 2021-08-05 2021-10-01 贵州百穗农特产品有限公司 一种超氧化物歧化酶的生产方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4943674A (en) * 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
US5538878A (en) * 1988-08-08 1996-07-23 Calgene, Inc. Superoxide dismutase expression in plants
EP0359617A3 (de) * 1988-09-02 1990-04-04 Plant Genetic Systems, N.V. Stress-tolerante Pflanzen
FR2651504B1 (fr) * 1989-08-11 1993-09-10 Biosem Plantes transgeniques appartenant a l'espece cucumis melo.
FR2716884B1 (fr) * 1994-03-03 1996-10-04 Bio Obtention Sc Extrait protéique de cucumis melo à activité antioxydante et procédé de préparation, composition cosmétique ou pharmaceutique ou composition alimentaire contenant un tel extrait.

Also Published As

Publication number Publication date
EP0952224B1 (de) 2004-06-23
JPH11341929A (ja) 1999-12-14
JP3307604B2 (ja) 2002-07-24
US6084152A (en) 2000-07-04
EP0952224A3 (de) 2001-04-04
EP0952224A2 (de) 1999-10-27

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