DE69907139T2 - Verfahren zur angiostatinrückfaltung - Google Patents

Verfahren zur angiostatinrückfaltung Download PDF

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Publication number
DE69907139T2
DE69907139T2 DE69907139T DE69907139T DE69907139T2 DE 69907139 T2 DE69907139 T2 DE 69907139T2 DE 69907139 T DE69907139 T DE 69907139T DE 69907139 T DE69907139 T DE 69907139T DE 69907139 T2 DE69907139 T2 DE 69907139T2
Authority
DE
Germany
Prior art keywords
angiostatin
refolding method
disclosed
refolding
subfragments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69907139T
Other languages
English (en)
Other versions
DE69907139D1 (de
Inventor
N Violand
O Polazzi
F Casperson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GD Searle LLC
Original Assignee
GD Searle LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GD Searle LLC filed Critical GD Searle LLC
Publication of DE69907139D1 publication Critical patent/DE69907139D1/de
Application granted granted Critical
Publication of DE69907139T2 publication Critical patent/DE69907139T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69907139T 1998-01-12 1999-01-11 Verfahren zur angiostatinrückfaltung Expired - Fee Related DE69907139T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7124798P 1998-01-12 1998-01-12
PCT/US1999/000048 WO1999035248A2 (en) 1998-01-12 1999-01-11 Method of refolding angiostatin

Publications (2)

Publication Number Publication Date
DE69907139D1 DE69907139D1 (de) 2003-05-28
DE69907139T2 true DE69907139T2 (de) 2004-01-29

Family

ID=22100170

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69907139T Expired - Fee Related DE69907139T2 (de) 1998-01-12 1999-01-11 Verfahren zur angiostatinrückfaltung

Country Status (8)

Country Link
EP (1) EP1047771B1 (de)
AT (1) ATE238414T1 (de)
AU (1) AU2101599A (de)
DE (1) DE69907139T2 (de)
DK (1) DK1047771T3 (de)
ES (1) ES2198879T3 (de)
PT (1) PT1047771E (de)
WO (1) WO1999035248A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1096945A4 (de) * 1998-07-14 2003-01-02 Bristol Myers Squibb Co Angiostatinfragmente, welche an lysin binden
US6723536B2 (en) 1999-12-03 2004-04-20 Entremed, Inc. Method of producing and purifying angiostatin
CA2367198A1 (en) * 1999-12-03 2001-06-07 Entremed, Inc. Method of producing and purifying angiostatin protein
IL152804A0 (en) 2000-05-16 2003-06-24 Bolder Biotechnology Inc Methods for refolding proteins containing free cysteine residues
AU2001285919B2 (en) 2000-09-05 2007-08-23 Jiangsu Simcere Pharmaceutical R&D Co., Ltd Recombinant endothelial cell growth inhibitors derived from a mammalian plasminogen
CN101730740A (zh) * 2006-09-29 2010-06-09 蛋白质组技术公司 用于生产重组纤溶酶原和纤溶酶多肽的方法
US8617531B2 (en) 2006-12-14 2013-12-31 Bolder Biotechnology, Inc. Methods of making proteins and peptides containing a single free cysteine

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2047063A1 (en) * 1991-07-15 1993-01-16 Warren C. Mackellar Method for folding tissue plasminogen activators and erivatives thereof
EP0910571B1 (de) * 1996-05-03 2005-07-20 Abbott Laboratories Antiangiogenische peptiden, dafür kodierende polynukleotide und verfahren zur hemmung der angiogenesis

Also Published As

Publication number Publication date
DE69907139D1 (de) 2003-05-28
EP1047771A2 (de) 2000-11-02
EP1047771B1 (de) 2003-04-23
ATE238414T1 (de) 2003-05-15
PT1047771E (pt) 2003-08-29
WO1999035248A3 (en) 1999-11-11
DK1047771T3 (da) 2003-08-11
AU2101599A (en) 1999-07-26
WO1999035248A2 (en) 1999-07-15
ES2198879T3 (es) 2004-02-01

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee