ATE238414T1 - Verfahren zur angiostatinrückfaltung - Google Patents

Verfahren zur angiostatinrückfaltung

Info

Publication number
ATE238414T1
ATE238414T1 AT99901279T AT99901279T ATE238414T1 AT E238414 T1 ATE238414 T1 AT E238414T1 AT 99901279 T AT99901279 T AT 99901279T AT 99901279 T AT99901279 T AT 99901279T AT E238414 T1 ATE238414 T1 AT E238414T1
Authority
AT
Austria
Prior art keywords
angiostatin
refolding
disclosed
subfragments
solubilizing
Prior art date
Application number
AT99901279T
Other languages
English (en)
Inventor
Bernard N Violand
Joseph O Polazzi
Gerald F Casperson
Original Assignee
Searle & Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Searle & Co filed Critical Searle & Co
Application granted granted Critical
Publication of ATE238414T1 publication Critical patent/ATE238414T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT99901279T 1998-01-12 1999-01-11 Verfahren zur angiostatinrückfaltung ATE238414T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7124798P 1998-01-12 1998-01-12
PCT/US1999/000048 WO1999035248A2 (en) 1998-01-12 1999-01-11 Method of refolding angiostatin

Publications (1)

Publication Number Publication Date
ATE238414T1 true ATE238414T1 (de) 2003-05-15

Family

ID=22100170

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99901279T ATE238414T1 (de) 1998-01-12 1999-01-11 Verfahren zur angiostatinrückfaltung

Country Status (8)

Country Link
EP (1) EP1047771B1 (de)
AT (1) ATE238414T1 (de)
AU (1) AU2101599A (de)
DE (1) DE69907139T2 (de)
DK (1) DK1047771T3 (de)
ES (1) ES2198879T3 (de)
PT (1) PT1047771E (de)
WO (1) WO1999035248A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1096945A4 (de) * 1998-07-14 2003-01-02 Bristol Myers Squibb Co Angiostatinfragmente, welche an lysin binden
US6723536B2 (en) 1999-12-03 2004-04-20 Entremed, Inc. Method of producing and purifying angiostatin
AU781204B2 (en) * 1999-12-03 2005-05-12 Children's Medical Center Corporation Method of producing and purifying angiostatin protein
IL152804A0 (en) 2000-05-16 2003-06-24 Bolder Biotechnology Inc Methods for refolding proteins containing free cysteine residues
JP2004508048A (ja) 2000-09-05 2004-03-18 カロリンスカ　イノベーションズ　アクティーエボラーグ 内皮細胞増殖阻害物質に関する材料と方法
CN101730740A (zh) * 2006-09-29 2010-06-09 蛋白质组技术公司 用于生产重组纤溶酶原和纤溶酶多肽的方法
EP2102355B1 (de) 2006-12-14 2016-03-02 Bolder Biotechnology, Inc. Proteine und peptide mit langer wirkung sowie verfahren zu ihrer herstellung und verwendung

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2047063A1 (en) * 1991-07-15 1993-01-16 Warren C. Mackellar Method for folding tissue plasminogen activators and erivatives thereof
BR9708911A (pt) * 1996-05-03 1999-08-03 Abbott Lab Composto fragmento de peptídeo processo par tratar uma doença em um paciente em necessidade de terapia antioangiogênese composição polinucleotídeo único ou de duplo-filamento isolado vetor e processo para produzir um fragmento de peptídeo de kringle 5 ou proteína de fusão de kringle 5 solúvel

Also Published As

Publication number Publication date
PT1047771E (pt) 2003-08-29
EP1047771B1 (de) 2003-04-23
DE69907139T2 (de) 2004-01-29
WO1999035248A2 (en) 1999-07-15
DK1047771T3 (da) 2003-08-11
DE69907139D1 (de) 2003-05-28
AU2101599A (en) 1999-07-26
EP1047771A2 (de) 2000-11-02
WO1999035248A3 (en) 1999-11-11
ES2198879T3 (es) 2004-02-01

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