DE69903337D1 - Ein screeningsystem auf zellbasis - Google Patents

Ein screeningsystem auf zellbasis

Info

Publication number
DE69903337D1
DE69903337D1 DE69903337T DE69903337T DE69903337D1 DE 69903337 D1 DE69903337 D1 DE 69903337D1 DE 69903337 T DE69903337 T DE 69903337T DE 69903337 T DE69903337 T DE 69903337T DE 69903337 D1 DE69903337 D1 DE 69903337D1
Authority
DE
Germany
Prior art keywords
cell
cells
screening system
based screening
kits
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69903337T
Other languages
English (en)
Other versions
DE69903337T2 (de
Inventor
A Guiliano
Gary Bright
Keith Olson
Sarah Burroughs-Tencza
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cellomics Inc
Original Assignee
Cellomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellomics Inc filed Critical Cellomics Inc
Publication of DE69903337D1 publication Critical patent/DE69903337D1/de
Application granted granted Critical
Publication of DE69903337T2 publication Critical patent/DE69903337T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C12N9/6475—Interleukin 1-beta convertase-like enzymes (3.4.22.10; 3.4.22.36; 3.4.22.63)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22056—Caspase-3 (3.4.22.56)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22059—Caspase-6 (3.4.22.59)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22061—Caspase-8 (3.4.22.61)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE69903337T 1998-10-30 1999-10-29 Ein screeningsystem auf zellbasis Expired - Lifetime DE69903337T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10630898P 1998-10-30 1998-10-30
US13607899P 1999-05-26 1999-05-26
PCT/US1999/025431 WO2000026408A2 (en) 1998-10-30 1999-10-29 A system for cell-based screening

Publications (2)

Publication Number Publication Date
DE69903337D1 true DE69903337D1 (de) 2002-11-07
DE69903337T2 DE69903337T2 (de) 2003-08-21

Family

ID=26803536

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69903337T Expired - Lifetime DE69903337T2 (de) 1998-10-30 1999-10-29 Ein screeningsystem auf zellbasis

Country Status (10)

Country Link
US (1) US20060094868A1 (de)
EP (1) EP1131471B1 (de)
JP (1) JP3863372B2 (de)
AT (1) ATE225406T1 (de)
AU (1) AU759180B2 (de)
CA (1) CA2348132C (de)
DE (1) DE69903337T2 (de)
DK (1) DK1131471T3 (de)
IL (1) IL142636A0 (de)
WO (1) WO2000026408A2 (de)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5989835A (en) 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US7853411B2 (en) 1997-02-27 2010-12-14 Cellomics, Inc. System for cell-based screening
US6759206B1 (en) 1997-02-27 2004-07-06 Cellomics, Inc. System for cell-based screening
US6416959B1 (en) 1997-02-27 2002-07-09 Kenneth Giuliano System for cell-based screening
US6743576B1 (en) 1999-05-14 2004-06-01 Cytokinetics, Inc. Database system for predictive cellular bioinformatics
US6651008B1 (en) 1999-05-14 2003-11-18 Cytokinetics, Inc. Database system including computer code for predictive cellular bioinformatics
US7151847B2 (en) 2001-02-20 2006-12-19 Cytokinetics, Inc. Image analysis of the golgi complex
AU7131900A (en) * 1999-06-21 2001-01-09 Cellomics, Inc. A system for cell-based screening
US6986993B1 (en) 1999-08-05 2006-01-17 Cellomics, Inc. System for cell-based screening
EP1305412A2 (de) 1999-10-14 2003-05-02 Clontech Laboratories Inc. Chromo/fluoroproteine aus anthozoa und verwendung der gleichen
AU2073801A (en) 1999-12-09 2001-06-18 Cellomics, Inc. A system for cell-based screening
FR2811681A1 (fr) * 2000-07-17 2002-01-18 Commissariat Energie Atomique Procede de criblage de la translocation nucleo-cytoplasmique de proteines sous l'effet d'un stimulus, d'elements y etant impliques et kits de mise en oeuvre
AU2002230415A1 (en) 2000-11-17 2002-05-27 Universal Imaging Corporation Rapidly changing dichroic beamsplitter
US7218764B2 (en) 2000-12-04 2007-05-15 Cytokinetics, Inc. Ploidy classification method
US6599694B2 (en) 2000-12-18 2003-07-29 Cytokinetics, Inc. Method of characterizing potential therapeutics by determining cell-cell interactions
US6956961B2 (en) 2001-02-20 2005-10-18 Cytokinetics, Inc. Extracting shape information contained in cell images
EP1379855A1 (de) 2001-02-20 2004-01-14 Cytokinetics Verfahren und gerät zur automatisierten zellbioinformatik
US7016787B2 (en) 2001-02-20 2006-03-21 Cytokinetics, Inc. Characterizing biological stimuli by response curves
WO2002073200A1 (en) 2001-03-12 2002-09-19 Cellomics, Inc. Methods to increase the capacity of high content cell-based screening assays
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
DE60234131D1 (de) 2001-08-01 2009-12-03 Cellomics Inc Neue fusionsproteine und molekulare bindungsassays
ES2329666T3 (es) 2001-12-19 2009-11-30 The University Of Chicago Proteinas fluorescentes de maduracion rapida y procedimientos de uso de las mismas.
US6942987B2 (en) * 2002-05-15 2005-09-13 Pharmacopeia Drug Discovery, Inc. Methods for measuring kinase activity
US7183066B2 (en) 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
DK1565559T3 (da) 2002-11-12 2011-08-29 Zakrytoe Aktsionernoe Obschestvo Evrogen Fluorescerende proteiner og chromoproteiner fra ikke aequorea hydrozoa arter samt fremgangsmåder til anvendelse deraf
EP1576157B1 (de) 2002-12-26 2010-07-21 Zakrytoe Aktsionernoe Obschestvo " Evrogen" Fluoreszenzproteine aus copepoda-spezies und verfahren zur verwendung davon
EP1646926A2 (de) 2003-07-18 2006-04-19 Cytokinetics, Inc. Charakterisierung biologischer stimuli durch ansprechkurven
US7235353B2 (en) 2003-07-18 2007-06-26 Cytokinetics, Inc. Predicting hepatotoxicity using cell based assays
US20050014217A1 (en) 2003-07-18 2005-01-20 Cytokinetics, Inc. Predicting hepatotoxicity using cell based assays
JP4755174B2 (ja) 2004-04-07 2011-08-24 ザ ユニヴァーシティー オヴ シカゴ 単量体赤色蛍光タンパク質
US7323318B2 (en) 2004-07-15 2008-01-29 Cytokinetics, Inc. Assay for distinguishing live and dead cells
EP1954713B1 (de) 2005-11-04 2012-11-14 Evrogen, JSC Modifizierte grünfluoreszierende proteine und verfahren zu deren anwendung
WO2008018905A2 (en) * 2006-01-17 2008-02-14 Cellumen, Inc. Method for predicting biological systems responses
US8563703B2 (en) 2006-01-25 2013-10-22 Evrogen IP Joint Stock Company Fluorescent proteins and methods for using same
ES2353081T3 (es) 2006-01-25 2011-02-25 Evrogen Ip Nuevas proteínas fluorescentes y métodos para usarlas.
JP5406019B2 (ja) 2006-05-17 2014-02-05 セルーメン、インコーポレイテッド 自動化組織分析のための方法
US20080032321A1 (en) * 2006-08-07 2008-02-07 General Electric Company System and methods for analyzing images of tissue samples
US8060348B2 (en) * 2006-08-07 2011-11-15 General Electric Company Systems for analyzing tissue samples
US8131476B2 (en) * 2006-08-07 2012-03-06 General Electric Company System and method for co-registering multi-channel images of a tissue micro array
US8680235B2 (en) 2006-09-22 2014-03-25 Stowers Institute For Medical Research Branchiostoma derived fluorescent proteins
EP2095119A2 (de) * 2006-11-10 2009-09-02 Cellumen, Inc. Biosensoren für protein-protein-interaktion und verfahren zu ihrer verwendung
US9557217B2 (en) 2007-02-13 2017-01-31 Bti Holdings, Inc. Universal multidetection system for microplates
US8679749B2 (en) 2007-11-01 2014-03-25 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
US10246492B2 (en) 2009-10-16 2019-04-02 Biomadison, Inc. Botulinum assay with synaptobrevin substrate moiety
EP2686688B1 (de) 2011-03-17 2019-05-08 Cernostics, Inc. Systeme und zusammensetzungen zur diagnose von barrett-ösophagen und verwendungsverfahren dafür
CH706326A2 (de) * 2012-03-14 2013-09-30 Tecan Trading Ag Verfahren und Mikroplatten-Reader zum Untersuchung von biologischen Zellen oder Zellkulturen.
EP2856117A4 (de) * 2012-05-29 2016-02-17 Univ Macquarie Bidirektionale abtastung für die lumineszenzmikroskopie
US10443089B2 (en) * 2013-02-26 2019-10-15 National Centre For Biological Sciences (Ncbs-Tifr) Methods of multiplexing DNA sensors and localizing DNA sensor
LT3404112T (lt) * 2013-10-25 2020-12-28 Biomadison, Inc. Fermento analizė su dublikuotais fluoroforais
KR102369298B1 (ko) 2014-04-29 2022-03-03 삼성디스플레이 주식회사 플렉서블 디스플레이 장치 및 그 제조방법
JP6802267B2 (ja) * 2015-05-29 2020-12-16 エコラボ ユーエスエー インコーポレイティド 飛翔昆虫を誘引し捕獲するための装置及び方法
WO2017091658A1 (en) 2015-11-25 2017-06-01 Cernostics, Inc. Methods of predicting progression of barrett's esophagus
JP6231709B1 (ja) * 2016-05-31 2017-11-15 シスメックス株式会社 蛍光画像分析装置および分析方法
US11428693B2 (en) 2017-03-16 2022-08-30 Baylor College Of Medicine Method of high throughput screening of chemical compounds suppressing nucleolar hypertrophy
GB2566516A (en) 2017-09-15 2019-03-20 Univ Oxford Innovation Ltd Electrochemical recognition and quantification of cytochrome c oxidase expression in bacteria
EP3467563A1 (de) * 2017-10-06 2019-04-10 Siemens Healthcare GmbH Mikroskopiervorrichtung
US20210181181A1 (en) * 2017-12-15 2021-06-17 The Regents Of The University Of Colorado, A Body Corporate Synthetic Fluorescent Protein Biosensors and Use Thereof in Drug Screening Methods
DE102019208833A1 (de) * 2019-06-18 2020-12-24 Robert Bosch Gmbh Verfahren zum Kennzeichnen von Pflanzenbildinformationen, insbesondere für landwirtschaftliche Zwecke
US20230054407A1 (en) * 2020-01-13 2023-02-23 Arizona Board Of Regents On Behalf Of The University Of Arizona Optical biopsy stain panels and methods of use

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4281061A (en) * 1979-07-27 1981-07-28 Syva Company Double antibody for enhanced sensitivity in immunoassay
NZ242739A (en) * 1991-05-24 1994-12-22 Arch Dev Corp Identification and purification of herpes protease nucleic acid segments and their use in the production of this protease
EP0541830A1 (de) * 1991-11-09 1993-05-19 ISP Van Dyk Inc. Absorptionsgrundlage enthaltend einen Sucroseester
US5997861A (en) * 1994-10-31 1999-12-07 Burstein Laboratories, Inc. Antiviral supramolecules containing target-binding molecules and therapeutic molecules bound to spectrin

Also Published As

Publication number Publication date
CA2348132A1 (en) 2000-05-11
DE69903337T2 (de) 2003-08-21
JP3863372B2 (ja) 2006-12-27
EP1131471A2 (de) 2001-09-12
AU759180B2 (en) 2003-04-10
DK1131471T3 (da) 2002-12-30
US20060094868A1 (en) 2006-05-04
IL142636A0 (en) 2002-03-10
WO2000026408A3 (en) 2000-09-14
ATE225406T1 (de) 2002-10-15
WO2000026408A2 (en) 2000-05-11
CA2348132C (en) 2003-08-12
JP2002528136A (ja) 2002-09-03
AU1240400A (en) 2000-05-22
EP1131471B1 (de) 2002-10-02

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