DE60027735D1 - Genetische screeningverfahren - Google Patents

Genetische screeningverfahren

Info

Publication number
DE60027735D1
DE60027735D1 DE60027735T DE60027735T DE60027735D1 DE 60027735 D1 DE60027735 D1 DE 60027735D1 DE 60027735 T DE60027735 T DE 60027735T DE 60027735 T DE60027735 T DE 60027735T DE 60027735 D1 DE60027735 D1 DE 60027735D1
Authority
DE
Germany
Prior art keywords
gene
cells
interest
identify
screen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60027735T
Other languages
English (en)
Inventor
Dan Canaani
Yoram Oron
Arnold Simons
Naomi Dafni
Iris Dotan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ramot at Tel Aviv University Ltd
Original Assignee
Ramot at Tel Aviv University Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ramot at Tel Aviv University Ltd filed Critical Ramot at Tel Aviv University Ltd
Application granted granted Critical
Publication of DE60027735D1 publication Critical patent/DE60027735D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
DE60027735T 1999-09-08 2000-09-07 Genetische screeningverfahren Expired - Lifetime DE60027735D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/391,444 US6569623B1 (en) 1999-09-08 1999-09-08 Genetic screening methods
PCT/IL2000/000535 WO2001018197A1 (en) 1999-09-08 2000-09-07 Genetic screening methods

Publications (1)

Publication Number Publication Date
DE60027735D1 true DE60027735D1 (de) 2006-06-08

Family

ID=23546623

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60027735T Expired - Lifetime DE60027735D1 (de) 1999-09-08 2000-09-07 Genetische screeningverfahren

Country Status (6)

Country Link
US (1) US6569623B1 (de)
EP (1) EP1214405B1 (de)
AT (1) ATE325190T1 (de)
AU (1) AU6862900A (de)
DE (1) DE60027735D1 (de)
WO (1) WO2001018197A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19956568A1 (de) 1999-01-30 2000-08-17 Roland Kreutzer Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
US7829693B2 (en) 1999-11-24 2010-11-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
DE10100586C1 (de) 2001-01-09 2002-04-11 Ribopharma Ag Verfahren zur Hemmung der Expression eines Ziegens
US8546143B2 (en) 2001-01-09 2013-10-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US7767802B2 (en) 2001-01-09 2010-08-03 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
US7423142B2 (en) 2001-01-09 2008-09-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
US7745418B2 (en) 2001-10-12 2010-06-29 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting viral replication
DE10163098B4 (de) 2001-10-12 2005-06-02 Alnylam Europe Ag Verfahren zur Hemmung der Replikation von Viren
DE10202419A1 (de) 2002-01-22 2003-08-07 Ribopharma Ag Verfahren zur Hemmung der Expression eines durch eine Chromosomen-Aberration entstandenen Zielgens
JP2010504507A (ja) * 2006-09-20 2010-02-12 インスティチュート・パスツール・コリア 細胞におけるターゲットタンパク質候補の発現を検出および／または定量する方法、ならびに小分子モデュレーターのターゲットタンパク質を同定する方法
EP1905827A1 (de) * 2006-09-20 2008-04-02 Institut Pasteur Korea Methode zur Detektion und/oder Quantifizierung der Expression von Zielproteinen in einer Zelle und Methode zur Identifizierung von Zielproteinen von Modulatoren kleiner Moleküle

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5882880A (en) * 1994-10-11 1999-03-16 Ramot University Authority For Applied And Industrial Development Ltd. Human checkpoint gene and gene for antisense RNA thereof
AU5442198A (en) * 1996-11-13 1998-06-03 Q.B.I. Enterprises Ltd. Gene identification method
EP1029072A4 (de) 1997-11-06 2004-04-07 Hutchinson Fred Cancer Res Verfahren zum erkennen von angriffszielen von medikamenten

Also Published As

Publication number Publication date
EP1214405A1 (de) 2002-06-19
ATE325190T1 (de) 2006-06-15
AU6862900A (en) 2001-04-10
EP1214405B1 (de) 2006-05-03
US6569623B1 (en) 2003-05-27
WO2001018197A1 (en) 2001-03-15

Similar Documents

Publication Publication Date Title
Gielen et al. Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)
Li et al. Gradient microfluidics enables rapid bacterial growth inhibition testing
CA2362117A1 (en) A system for cell-based screening
DK1131471T3 (da) Et system til cellebaseret screening
DE60140120D1 (de) Toxizitätstypisierung unter verwendung von leberstammzellen
WO2001009301A3 (en) Nucleic acids and polypeptides of invertebrate twik channels and methods of use
DE60235005D1 (de) Nachweis der instabilität von mikrosatelliten und
DE60027735D1 (de) Genetische screeningverfahren
DE60031997D1 (de) Umkehrtransfektionsverfahren
IL147793A0 (en) Optical system analysis of cells
DE69942459D1 (de) Isolierte nukleinsäuremoleküle die t-zellen induzierbare faktoren kodieren (tif), kodierte proteine und ihre anwendung
BRPI0412845A (pt) herpes-vìrus, uso de um vìrus, composição farmacêutica, e, método de tratamento de um tumor em um indivìduo em necessidade do mesmo
ATE408824T1 (de) Miniaturisierte zellenanordnung und verfahren und vorrichtung zum screening mittels zellen
AU2001245945A1 (en) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
WO2001011341A3 (en) A system for cell-based screening
DK0921857T3 (da) Flerbröndsplader med lav baggrund til fluorescensmålinger af biologiske og biokemiske pröver
DE60210485D1 (de) Verfahren zur bestimmung des vorhandenseins von bakterien, die gegen zell-lysierende antibiotika resistent sind
DE69533144D1 (de) Methode zur zellyse
ATE321138T1 (de) Polyketide, ihre herstellung, und darin benutzte materialien
GB0509611D0 (en) Method and device for imaging a sample
WO2006039630A3 (en) Materials and methods for identifying compounds that modulate the cell cycle
WO2004092413A3 (en) High throughput functional genomic screening methods for osteoarthritis
WO2005052182A3 (en) A method of analyzing plasma membrane protein content of cells
CA2222909A1 (en) Detection and quantification of one or more nucleotide sequence target analytes in a sample using spatially localized target analyte replication
WO2001038359A3 (en) Drosophila nicotinic acetylcholine receptor

Legal Events

Date Code Title Description
8332 No legal effect for de