WO2005052182A3 - A method of analyzing plasma membrane protein content of cells - Google Patents

A method of analyzing plasma membrane protein content of cells Download PDF

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Publication number
WO2005052182A3
WO2005052182A3 PCT/IL2004/001085 IL2004001085W WO2005052182A3 WO 2005052182 A3 WO2005052182 A3 WO 2005052182A3 IL 2004001085 W IL2004001085 W IL 2004001085W WO 2005052182 A3 WO2005052182 A3 WO 2005052182A3
Authority
WO
WIPO (PCT)
Prior art keywords
plasma membrane
cells
protein content
membrane protein
present
Prior art date
Application number
PCT/IL2004/001085
Other languages
French (fr)
Other versions
WO2005052182A2 (en
Inventor
Michal Linial
Alex Inberg
Yaniv Bledi
Original Assignee
Yissum Res Dev Co
Michal Linial
Alex Inberg
Yaniv Bledi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Res Dev Co, Michal Linial, Alex Inberg, Yaniv Bledi filed Critical Yissum Res Dev Co
Publication of WO2005052182A2 publication Critical patent/WO2005052182A2/en
Publication of WO2005052182A3 publication Critical patent/WO2005052182A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

The present invention is of a method of characterizing proteins present on the plasma membrane of live cells which can be used in the identification of diagnostic markers and potentials drugs. Specifically, the present invention can be used to identify drug targets useful in treating disorders, such as cancer, which are associated with abnormal representation of cell surface proteins.
PCT/IL2004/001085 2003-11-26 2004-11-25 A method of analyzing plasma membrane protein content of cells WO2005052182A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52488503P 2003-11-26 2003-11-26
US60/524,885 2003-11-26

Publications (2)

Publication Number Publication Date
WO2005052182A2 WO2005052182A2 (en) 2005-06-09
WO2005052182A3 true WO2005052182A3 (en) 2005-07-07

Family

ID=34632938

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2004/001085 WO2005052182A2 (en) 2003-11-26 2004-11-25 A method of analyzing plasma membrane protein content of cells

Country Status (1)

Country Link
WO (1) WO2005052182A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112480235B (en) * 2020-12-14 2022-07-15 上海交通大学 Bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL, and preparation method and application thereof

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10254601A1 (en) 2002-11-22 2004-06-03 Ganymed Pharmaceuticals Ag Gene products differentially expressed in tumors and their use
DE10341812A1 (en) * 2003-09-10 2005-04-07 Ganymed Pharmaceuticals Ag Differentially expressed in tumors gene products and their use
DE102004024617A1 (en) 2004-05-18 2005-12-29 Ganymed Pharmaceuticals Ag Differentially expressed in tumors gene products and their use
EP1790664A1 (en) 2005-11-24 2007-05-30 Ganymed Pharmaceuticals AG Monoclonal antibodies against claudin-18 for treatment of cancer
US7879799B2 (en) 2006-08-10 2011-02-01 Institute For Systems Biology Methods for characterizing glycoproteins and generating antibodies for same
WO2013167153A1 (en) 2012-05-09 2013-11-14 Ganymed Pharmaceuticals Ag Antibodies useful in cancer diagnosis
WO2023049789A2 (en) * 2021-09-24 2023-03-30 Nitto Denko Corporation Yeast cells with reduced propensity to degrade acrylic acid

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002100892A1 (en) * 2001-05-29 2002-12-19 Regents Of The University Of Michigan Systems and methods for the analysis of proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002100892A1 (en) * 2001-05-29 2002-12-19 Regents Of The University Of Michigan Systems and methods for the analysis of proteins

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BLEDI YANIV ET AL: "PROCEED: A proteomic method for analysing plasma membrane proteins in living mammalian cells.", BRIEFINGS IN FUNCTIONAL GENOMICS & PROTEOMICS, vol. 2, no. 3, October 2003 (2003-10-01), pages 254 - 265, XP002327557, ISSN: 1473-9550 *
BLONDER JOSIP ET AL: "Enrichment of integral membrane proteins for proteomic analysis using liquid chromatography-tandem mass spectrometry", JOURNAL OF PROTEOME RESEARCH, vol. 1, no. 4, July 2002 (2002-07-01), pages 351 - 360, XP002327558, ISSN: 1535-3893 *
WASHBURN MICHAEL P ET AL: "Large-scale analysis of the yeast proteome by multidimensional protein identification technology", NATURE BIOTECHNOLOGY, vol. 19, no. 3, March 2001 (2001-03-01), pages 242 - 247, XP002327559, ISSN: 1087-0156 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112480235B (en) * 2020-12-14 2022-07-15 上海交通大学 Bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL, and preparation method and application thereof

Also Published As

Publication number Publication date
WO2005052182A2 (en) 2005-06-09

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