DE69901432T2 - Selektive konzentration und anreicherungssortierung von flusszytometertropfen, basierend auf der analyse von tröpfchenvorläuferregionen - Google Patents
Selektive konzentration und anreicherungssortierung von flusszytometertropfen, basierend auf der analyse von tröpfchenvorläuferregionenInfo
- Publication number
- DE69901432T2 DE69901432T2 DE69901432T DE69901432T DE69901432T2 DE 69901432 T2 DE69901432 T2 DE 69901432T2 DE 69901432 T DE69901432 T DE 69901432T DE 69901432 T DE69901432 T DE 69901432T DE 69901432 T2 DE69901432 T2 DE 69901432T2
- Authority
- DE
- Germany
- Prior art keywords
- droplet
- precursor regions
- adjacent
- regions
- sorting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000002243 precursor Substances 0.000 claims description 105
- 239000012530 fluid Substances 0.000 claims description 83
- 238000000034 method Methods 0.000 claims description 18
- 238000011144 upstream manufacturing Methods 0.000 claims description 12
- 210000000601 blood cell Anatomy 0.000 claims description 11
- 230000003287 optical effect Effects 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 137
- 239000002245 particle Substances 0.000 description 40
- 230000008929 regeneration Effects 0.000 description 24
- 238000011069 regeneration method Methods 0.000 description 24
- 238000003306 harvesting Methods 0.000 description 12
- 239000002699 waste material Substances 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 7
- 239000011324 bead Substances 0.000 description 6
- 230000009977 dual effect Effects 0.000 description 6
- 238000002512 chemotherapy Methods 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 230000007717 exclusion Effects 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000012545 processing Methods 0.000 description 3
- 238000007600 charging Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000012958 reprocessing Methods 0.000 description 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000007786 electrostatic charging Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/031,484 US6281018B1 (en) | 1998-02-26 | 1998-02-26 | Selective purification and enrichment sorting of flow cytometer droplets based upon analysis of droplet precursor regions |
| PCT/US1999/002810 WO1999044035A1 (en) | 1998-02-26 | 1999-02-09 | Selective purification and enrichment sorting of flow cytometer droplets based upon analysis of droplet precursor regions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69901432D1 DE69901432D1 (de) | 2002-06-13 |
| DE69901432T2 true DE69901432T2 (de) | 2002-11-28 |
Family
ID=21859713
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69901432T Expired - Lifetime DE69901432T2 (de) | 1998-02-26 | 1999-02-09 | Selektive konzentration und anreicherungssortierung von flusszytometertropfen, basierend auf der analyse von tröpfchenvorläuferregionen |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US6281018B1 (enExample) |
| EP (1) | EP1058831B1 (enExample) |
| JP (1) | JP4091255B2 (enExample) |
| DE (1) | DE69901432T2 (enExample) |
| WO (1) | WO1999044035A1 (enExample) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2264428B1 (en) | 1997-01-31 | 2017-05-03 | Xy, Llc | Optical apparatus with focussing reflector for converging radiation onto a flow of particles |
| US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| US6071689A (en) | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
| AUPQ629100A0 (en) * | 2000-03-16 | 2000-04-15 | Btf Pty Ltd | Process for preparing controlled samples of particles such as microorganisms and cells |
| AU2002237689B2 (en) | 2000-11-29 | 2008-01-10 | Xy, Llc. | System to separate frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations |
| US6793642B2 (en) | 2001-05-07 | 2004-09-21 | Biomed Solutions, Llc | Flow cytometer |
| WO2002092161A1 (en) * | 2001-05-10 | 2002-11-21 | Biophan, Llc | Miniaturized particle analyzer |
| US6880414B2 (en) * | 2002-08-08 | 2005-04-19 | Becton Dickinson And Company | Sort block and liquid collection device for sorting flow cytometer |
| US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
| US7201875B2 (en) * | 2002-09-27 | 2007-04-10 | Becton Dickinson And Company | Fixed mounted sorting cuvette with user replaceable nozzle |
| EP2305171B1 (en) | 2003-03-28 | 2021-12-29 | Inguran, LLC | Apparatus and methods for providing sex-sorted animal sperm |
| US20100065734A1 (en) * | 2006-12-04 | 2010-03-18 | The University Of Queensland | Particle sorting apparatus and method |
| US7678883B2 (en) * | 2007-07-25 | 2010-03-16 | E.I. Du Pont De Nemours And Company | Solubility tags for the expression and purification of bioactive peptides |
| JP4572973B2 (ja) * | 2008-06-16 | 2010-11-04 | ソニー株式会社 | マイクロチップ及びマイクロチップにおける送流方法 |
| EP2157599A1 (en) * | 2008-08-21 | 2010-02-24 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | Method and apparatus for identification of biological material |
| JP5487638B2 (ja) * | 2009-02-17 | 2014-05-07 | ソニー株式会社 | 微小粒子分取のための装置及びマイクロチップ |
| US20110114190A1 (en) * | 2009-11-16 | 2011-05-19 | The Hong Kong University Of Science And Technology | Microfluidic droplet generation and/or manipulation with electrorheological fluid |
| EP3508860A1 (en) * | 2010-02-09 | 2019-07-10 | Microjet Corporation | Discharge device for liquid material including particle-like bodies |
| US9347933B2 (en) | 2011-12-20 | 2016-05-24 | Becton, Dickinson And Company | System and method to improve yield of sorted particles |
| US9108196B1 (en) | 2012-01-24 | 2015-08-18 | Stratedigm, Inc. | Method and apparatus for control of fluid flow or fluid suspended particle flow in a microfluidic channel |
| US10190959B2 (en) | 2012-02-09 | 2019-01-29 | Beckman Coulter, Inc. | Sorting flow cytometer |
| CN104508461B (zh) * | 2012-06-22 | 2018-12-25 | 生物辐射实验室股份有限公司 | 流式细胞仪中具有减少污染的多向分类 |
| WO2014047206A1 (en) | 2012-09-18 | 2014-03-27 | Cytonome/St, Llc | Flow cell for particle sorting |
| JP6186812B2 (ja) * | 2013-04-04 | 2017-08-30 | ソニー株式会社 | 粒子分取装置及び粒子分取方法 |
| FR3050269B1 (fr) * | 2016-04-15 | 2018-05-11 | Ecole Superieure De Physique Et De Chimie Industrielles De La Ville De Paris (Espci) | Procede de selection et de recuperation de produits et systeme associe |
| JP6922901B2 (ja) * | 2016-05-17 | 2021-08-18 | ソニーグループ株式会社 | 粒子分取装置及び粒子分取方法 |
| WO2018213643A1 (en) | 2017-05-18 | 2018-11-22 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| US10610865B2 (en) | 2017-08-22 | 2020-04-07 | 10X Genomics, Inc. | Droplet forming devices and system with differential surface properties |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| US10914671B2 (en) * | 2018-04-27 | 2021-02-09 | Becton, Dickinson And Company | Flow cytometers having enclosed droplet sorters with controlled aerosol content and methods of using the same |
| US11085868B2 (en) * | 2019-02-08 | 2021-08-10 | Becton, Dickinson And Company | Droplet sorting decision modules, systems and methods of use thereof |
| JP2021076455A (ja) * | 2019-11-07 | 2021-05-20 | ソニー株式会社 | 分取制御装置、該分取制御装置を用いた粒子分取装置及び粒子分取システム、並びに分取制御方法、及び制御プログラム |
| JP2022017705A (ja) * | 2020-07-14 | 2022-01-26 | ソニーグループ株式会社 | 微小粒子分取装置、微小粒子分取方法、プログラム、及び微小粒子分取システム |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3675768A (en) | 1969-03-17 | 1972-07-11 | Gildardo Legorreta Sanchez | Method and apparatus for classifying and segregating particles with electrical and optical means |
| US3826364A (en) | 1972-05-22 | 1974-07-30 | Univ Leland Stanford Junior | Particle sorting method and apparatus |
| JPS5319891A (en) * | 1976-06-10 | 1978-02-23 | Coulter Electronics | Method and apparatus for folling drop formation and separation |
| US4097373A (en) * | 1977-03-23 | 1978-06-27 | John Caldwell Allred | High speed particle sorter using a field emission electrode |
| US4347935A (en) * | 1979-05-16 | 1982-09-07 | The United States Of America As Represented By The United States Department Of Energy | Method and apparatus for electrostatically sorting biological cells |
| US4691829A (en) * | 1980-11-03 | 1987-09-08 | Coulter Corporation | Method of and apparatus for detecting change in the breakoff point in a droplet generation system |
| US4447883A (en) | 1981-05-26 | 1984-05-08 | Technicon Instruments Corporation | Coincidence-error correcting apparatus and method |
| US4667830A (en) | 1981-06-15 | 1987-05-26 | The Board Of Trustees Of The Leland Stanford Junior University | Method and means for sorting individual particles into containers for culturing, cloning, analysis, or the like |
| US4499052A (en) * | 1982-08-30 | 1985-02-12 | Becton, Dickinson And Company | Apparatus for distinguishing multiple subpopulations of cells |
| JPS59174742A (ja) | 1983-03-25 | 1984-10-03 | Agency Of Ind Science & Technol | 微小粒子を区分又は選別する方法及びその装置 |
| US4538733A (en) * | 1983-10-14 | 1985-09-03 | Becton, Dickinson And Company | Particle sorter with neutralized collection wells and method of using same |
| US4981580A (en) | 1989-05-01 | 1991-01-01 | Coulter Corporation | Coincidence arbitration in a flow cytomery sorting system |
| US5204884A (en) * | 1991-03-18 | 1993-04-20 | University Of Rochester | System for high-speed measurement and sorting of particles |
| US5199576A (en) * | 1991-04-05 | 1993-04-06 | University Of Rochester | System for flexibly sorting particles |
-
1998
- 1998-02-26 US US09/031,484 patent/US6281018B1/en not_active Expired - Lifetime
-
1999
- 1999-02-09 JP JP2000533735A patent/JP4091255B2/ja not_active Expired - Lifetime
- 1999-02-09 DE DE69901432T patent/DE69901432T2/de not_active Expired - Lifetime
- 1999-02-09 WO PCT/US1999/002810 patent/WO1999044035A1/en not_active Ceased
- 1999-02-09 EP EP99906853A patent/EP1058831B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002505422A (ja) | 2002-02-19 |
| WO1999044035A1 (en) | 1999-09-02 |
| JP4091255B2 (ja) | 2008-05-28 |
| EP1058831B1 (en) | 2002-05-08 |
| DE69901432D1 (de) | 2002-06-13 |
| EP1058831A1 (en) | 2000-12-13 |
| US6281018B1 (en) | 2001-08-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |