DE69831083D1 - Reprimiertes trans-aktivatorsystem zur charakterisierung von protein-protein interaktionen - Google Patents

Reprimiertes trans-aktivatorsystem zur charakterisierung von protein-protein interaktionen

Info

Publication number
DE69831083D1
DE69831083D1 DE69831083T DE69831083T DE69831083D1 DE 69831083 D1 DE69831083 D1 DE 69831083D1 DE 69831083 T DE69831083 T DE 69831083T DE 69831083 T DE69831083 T DE 69831083T DE 69831083 D1 DE69831083 D1 DE 69831083D1
Authority
DE
Germany
Prior art keywords
bait
protein
prey
fusion proteins
fusion protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69831083T
Other languages
English (en)
Other versions
DE69831083T2 (de
Inventor
Ivan Sadowski
Martin Hirst
John Rohde
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Interomex Biopharmaceuticals Inc
Original Assignee
Interomex Biopharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Interomex Biopharmaceuticals Inc filed Critical Interomex Biopharmaceuticals Inc
Publication of DE69831083D1 publication Critical patent/DE69831083D1/de
Application granted granted Critical
Publication of DE69831083T2 publication Critical patent/DE69831083T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69831083T 1997-09-09 1998-09-08 Reprimiertes trans-aktivatorsystem zur charakterisierung von protein-protein interaktionen Expired - Fee Related DE69831083T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US926623 1997-09-09
US08/926,623 US5885779A (en) 1997-09-09 1997-09-09 Repressed trans-activator system for characterization of protein-protein interactions
PCT/CA1998/000847 WO1999013069A1 (en) 1997-09-09 1998-09-08 Repressed trans-activator system for characterization of protein-protein interactions

Publications (2)

Publication Number Publication Date
DE69831083D1 true DE69831083D1 (de) 2005-09-08
DE69831083T2 DE69831083T2 (de) 2006-06-22

Family

ID=25453469

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69831083T Expired - Fee Related DE69831083T2 (de) 1997-09-09 1998-09-08 Reprimiertes trans-aktivatorsystem zur charakterisierung von protein-protein interaktionen

Country Status (8)

Country Link
US (1) US5885779A (de)
EP (1) EP1012265B1 (de)
JP (1) JP2001515718A (de)
AT (1) ATE301188T1 (de)
AU (1) AU748706B2 (de)
CA (1) CA2303416C (de)
DE (1) DE69831083T2 (de)
WO (1) WO1999013069A1 (de)

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US7393682B1 (en) 1993-03-19 2008-07-01 The Johns Hopkins University School Of Medicine Polynucleotides encoding promyostatin polypeptides
US5830751A (en) * 1996-05-01 1998-11-03 The Johns Hopkins University Genetic assays and strains using human TP53
US5948620A (en) * 1996-08-05 1999-09-07 Amersham International Plc Reverse two-hybrid system employing post-translation signal modulation
US6891082B2 (en) 1997-08-01 2005-05-10 The Johns Hopkins University School Of Medicine Transgenic non-human animals expressing a truncated activintype II receptor
US7745142B2 (en) * 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7632651B2 (en) * 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US20050227294A1 (en) * 1997-09-15 2005-10-13 Molecular Devices Corporation Molecular modification assays involving lipids
US6297018B1 (en) 1998-04-17 2001-10-02 Ljl Biosystems, Inc. Methods and apparatus for detecting nucleic acid polymorphisms
US6982431B2 (en) * 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US5985575A (en) * 1998-05-20 1999-11-16 Wisconsin Alumni Research Foundation Tethered function assay for protein function
CA2256121A1 (en) * 1998-06-08 1999-12-08 Hong Wang Cyclin-dependent kinase inhibitors as plant growth regulators
WO2000005417A1 (en) * 1998-07-23 2000-02-03 Small Molecule Therapeutics, Inc. Methods and compositions for the determination of protein function and identification of modulators thereof
AU756617B2 (en) * 1998-07-28 2003-01-16 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Leptin-mediated gene-induction
US8030546B2 (en) * 1998-09-22 2011-10-04 Mendel Biotechnology, Inc. Biotic and abiotic stress tolerance in plants
US20070226839A1 (en) * 1999-11-17 2007-09-27 Mendel Biotechnology Biotic and abiotic stress tolerance in plants
US6890726B1 (en) 1999-04-06 2005-05-10 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
US6287813B1 (en) * 1999-04-23 2001-09-11 Cistronics Cell Technology Gmbh Antibiotic-based gene regulation system
US6864355B1 (en) 2000-05-02 2005-03-08 Yale University Inhibition of NF-κB activation by blockade of IKKβ-NEMO interactions at the NEMO binding domain
US7186505B1 (en) * 1999-11-29 2007-03-06 Stil Biotechnologies Ltd. Expression systems and methods for detecting and isolating polypeptides regulating signal transduction pathways
FR2801902B1 (fr) * 1999-12-01 2005-03-04 Centre Nat Rech Scient Procedes de criblages fonctionnels d'agents aptes a moduler l'activite des complexes ubiquitine-ligases scf et leurs applications
US20030044787A1 (en) * 2000-05-16 2003-03-06 Joung J. Keith Methods and compositions for interaction trap assays
DE60112282T2 (de) * 2000-05-22 2006-04-20 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw. Auf rezeptorgrundlage arbeitende screening-verfahren für protein wechselwirkungen
US20030027286A1 (en) * 2000-09-06 2003-02-06 Robert Haselbeck Bacterial promoters and methods of use
US6787321B1 (en) * 2000-10-13 2004-09-07 The Regents Of The University Of California Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products
AU2002227153B2 (en) 2000-12-08 2008-01-24 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6551786B2 (en) * 2001-01-04 2003-04-22 Myriad Genetics, Inc. Screen assay for selecting protein-protein interaction modulators
WO2003004643A2 (en) * 2001-07-03 2003-01-16 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Reversed mammalian protein-protein interaction trap
ES2328796T3 (es) 2001-03-14 2009-11-18 Myriad Genetics, Inc. Interaccion tsg101-gag y uso de la misma.
US6841352B2 (en) * 2001-06-29 2005-01-11 Myriad Genetics, Inc. Mating-based method for detecting protein—protein interaction
US20050032197A1 (en) * 2001-11-06 2005-02-10 Pelletier Marc F. Membrane protein interaction assays
US20040067512A1 (en) * 2001-11-09 2004-04-08 Neurogenetics, Inc. Single nucleotide polymorphisms and mutations on Alpha-2-Macroglobulin
US20030162202A1 (en) * 2001-11-09 2003-08-28 Becker Kenneth David Single nucleotide polymorphisms and mutations on Alpha-2-Macroglobulin
DE10211063A1 (de) * 2002-03-13 2003-10-09 Axaron Bioscience Ag Neue Verfahren zur Detektion und Analyse von Protein-Interaktionen in vivo
DE10211653A1 (de) * 2002-03-15 2003-10-02 Klaus Pfizenmaier Filament-Rekrutierung fluoreszierender Proteine zur Analyse und Identifikation von Protein-Protein-Interaktionen: FIT (Filament-based Interaction Trap) analysis
US7786287B2 (en) * 2002-04-16 2010-08-31 Merck & Co., Inc. Hepatitis C virus assay systems
US7229762B2 (en) * 2002-10-15 2007-06-12 Duke University Medical Center Proteomic screening for redox state dependent protein—protein interactions
WO2005085467A1 (ja) * 2004-03-05 2005-09-15 Japan Science And Technology Agency タンパク質複合体検出方法、およびタンパク質複合体検出キット
EP1582590A1 (de) * 2004-04-02 2005-10-05 Aptanomics Sequentielles intrazelluläres Screening-Verfahren
FR2877010B1 (fr) * 2004-10-25 2007-03-16 Aventis Pharma Sa Systeme double-hybride base sur la mise sous silence de genes par interference transcriptionnelle
CA2585549A1 (en) * 2004-11-18 2006-05-26 Vib Vzw Novel type leptin receptor antagonist
JP2009514529A (ja) * 2005-10-31 2009-04-09 ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ Ｔｒｐｍ８およびカルモジュリンのポリペプチド複合体ならびにそれらのその用途
US20080301836A1 (en) * 2007-05-17 2008-12-04 Mendel Biotechnology, Inc. Selection of transcription factor variants
US8383589B2 (en) 2007-07-09 2013-02-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Aegyptin and uses thereof
KR20100048224A (ko) * 2008-10-30 2010-05-11 삼성전자주식회사 갈락토오스의 대사 이용율을 증대시키는 유전자, 이를 포함하는 재조합 벡터 및 재조합 미생물
WO2012170742A2 (en) 2011-06-07 2012-12-13 University Of Hawaii Treatment and prevention of cancer with hmgb1 antagonists
WO2012170740A2 (en) 2011-06-07 2012-12-13 University Of Hawaii Biomarker of asbestos exposure and mesothelioma
GB201820863D0 (en) 2018-12-20 2019-02-06 Univ Bath Screening assay

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
JP3537141B2 (ja) * 1992-10-30 2004-06-14 ザ ゼネラル ホスピタル コーポレーション 新種蛋白質分離のための相互作用を用いる補捉システム
US5866755A (en) * 1993-06-14 1999-02-02 Basf Aktiengellschaft Animals transgenic for a tetracycline-regulated transcriptional inhibitor
DE19502584C2 (de) * 1994-01-28 1999-08-12 Medigene Ag Ges Fuer Molekular Verfahren zur Bestimmung der Aktivität eines regulatorischen Faktors sowie Verwendung dieses Verfahrens
US5525490A (en) * 1994-03-29 1996-06-11 Onyx Pharmaceuticals, Inc. Reverse two-hybrid method
ATE352638T1 (de) * 1995-04-11 2007-02-15 Gen Hospital Corp REVERSE ßTWO-HYBRIDß-SYSTEME
EP0790304A1 (de) * 1995-12-21 1997-08-20 AMERSHAM INTERNATIONAL plc Auf Nachweismethoden bezogene Verbesserungen

Also Published As

Publication number Publication date
CA2303416C (en) 2008-11-25
EP1012265B1 (de) 2005-08-03
US5885779A (en) 1999-03-23
JP2001515718A (ja) 2001-09-25
AU748706B2 (en) 2002-06-13
CA2303416A1 (en) 1999-03-18
AU9057398A (en) 1999-03-29
EP1012265A1 (de) 2000-06-28
WO1999013069A1 (en) 1999-03-18
ATE301188T1 (de) 2005-08-15
DE69831083T2 (de) 2006-06-22

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee