DE69738587T2 - Einkapselung von polypeptiden in die stärkematrix - Google Patents
Einkapselung von polypeptiden in die stärkematrix Download PDFInfo
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- DE69738587T2 DE69738587T2 DE69738587T DE69738587T DE69738587T2 DE 69738587 T2 DE69738587 T2 DE 69738587T2 DE 69738587 T DE69738587 T DE 69738587T DE 69738587 T DE69738587 T DE 69738587T DE 69738587 T2 DE69738587 T2 DE 69738587T2
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- starch
- polypeptide
- hybrid polypeptide
- plant
- hybrid
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- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920000724 poly(L-arginine) polymer Polymers 0.000 description 1
- 108010011110 polyarginine Proteins 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000013930 proline Nutrition 0.000 description 1
- 125000001500 prolyl group Chemical class [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 210000003370 receptor cell Anatomy 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 229940102127 rubidium chloride Drugs 0.000 description 1
- 239000012146 running buffer Substances 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 239000006163 transport media Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 238000010626 work up procedure Methods 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Fodder In General (AREA)
- Heterocyclic Compounds That Contain Two Or More Ring Oxygen Atoms (AREA)
- Polyoxymethylene Polymers And Polymers With Carbon-To-Carbon Bonds (AREA)
- Details Of Resistors (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2685596P | 1996-09-30 | 1996-09-30 | |
| US26855P | 1996-09-30 | ||
| PCT/US1997/017555 WO1998014601A1 (en) | 1996-09-30 | 1997-09-30 | Encapsulation of polypeptides within the starch matrix |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69738587D1 DE69738587D1 (de) | 2008-04-30 |
| DE69738587T2 true DE69738587T2 (de) | 2009-04-30 |
Family
ID=21834174
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69738587T Expired - Lifetime DE69738587T2 (de) | 1996-09-30 | 1997-09-30 | Einkapselung von polypeptiden in die stärkematrix |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US6107060A (enExample) |
| EP (1) | EP0935665B1 (enExample) |
| JP (1) | JP2001505412A (enExample) |
| KR (1) | KR20000048782A (enExample) |
| CN (1) | CN1195861C (enExample) |
| AT (1) | ATE389725T1 (enExample) |
| AU (1) | AU730427B2 (enExample) |
| BR (1) | BR9713242A (enExample) |
| CA (1) | CA2265514A1 (enExample) |
| DE (1) | DE69738587T2 (enExample) |
| IL (1) | IL129158A0 (enExample) |
| NZ (1) | NZ334637A (enExample) |
| RU (1) | RU2268301C9 (enExample) |
| WO (1) | WO1998014601A1 (enExample) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6960457B1 (en) * | 1997-09-04 | 2005-11-01 | Stanford University | Reversible immobilization of arginine-tagged moieties on a silicate surface |
| DE19820607A1 (de) * | 1998-05-08 | 1999-11-11 | Hoechst Schering Agrevo Gmbh | Nucleinsäuremoleküle codierend Enzyme aus Weizen, die an der Stärkesynthese beteiligt sind |
| US6392120B1 (en) * | 1998-07-28 | 2002-05-21 | E. I. Du Pont De Nemours And Company | Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops |
| AUPQ005299A0 (en) * | 1999-04-29 | 1999-05-27 | Commonwealth Scientific And Industrial Research Organisation | Novel genes encoding wheat starch synthases and uses therefor |
| FR2793806B1 (fr) * | 1999-05-21 | 2003-04-25 | Centre Nat Rech Scient | Grains d'amidon contenant un polypeptide recombinant d'interet, leur procede d'obtention, et leurs utilisations |
| US7135619B1 (en) * | 1999-06-11 | 2006-11-14 | Wageningen Universiteit | Expression in plants of starch binding domains and/or of protein-fusions containing starch binding domains |
| AU1122701A (en) * | 1999-10-29 | 2001-05-14 | National Research Council Of Canada | Starch branching enzymes |
| US7041484B1 (en) | 1999-10-29 | 2006-05-09 | National Research Council Of Canada | Starch branching enzymes |
| US20040107461A1 (en) * | 2001-03-30 | 2004-06-03 | Padma Commuri | Glucan chain length domains |
| EP2216405A1 (en) | 2002-05-03 | 2010-08-11 | Monsanto Technology LLC | Speed specific USP promoters for expressing genes in plants |
| EP1648996B1 (en) | 2003-06-25 | 2012-03-14 | Novozymes A/S | Enzymes for starch processing |
| ES2517245T3 (es) | 2003-06-25 | 2014-11-03 | Novozymes A/S | Enzimas para el tratamiento de almidón |
| JP4923171B2 (ja) | 2003-06-30 | 2012-04-25 | コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション | 分枝酵素の活性を改変した小麦、ならびにそれから得たデンプンおよびデンプン含有品 |
| EP1692289B1 (en) | 2003-10-27 | 2012-01-25 | Commonwealth Scientific And Industrial Research Organisation | Rice and products thereof having starch with an increased proportion of amylose |
| US9756798B2 (en) | 2004-11-19 | 2017-09-12 | Patti D. Rubin | Burrow filling compressed growing medium |
| US20060107589A1 (en) | 2004-11-19 | 2006-05-25 | Rubin Patti D | Compressed growing medium |
| US8841091B2 (en) | 2004-12-22 | 2014-09-23 | Novozymes Als | Enzymes for starch processing |
| DK1831384T3 (en) | 2004-12-22 | 2015-11-23 | Novozymes As | Polypeptides with glucoamylase activity and polynucleotides encoding them. |
| FR2911608B1 (fr) * | 2007-01-23 | 2009-04-03 | Centre Nat Rech Scient | Nouvelles compositions vaccinales anti paludique et ses utilisations. |
| US20090113791A1 (en) | 2007-10-29 | 2009-05-07 | Oms Investments, Inc. | Compressed Coconut Coir Pith Granules and Methods for the Production and use Thereof |
| ES2377617B1 (es) * | 2010-08-31 | 2012-11-23 | Iden Biotechnology, S.L. | Procedimiento para la producción y purificación de proteínas recombinantes en plantas. |
| GB201311272D0 (en) | 2013-06-25 | 2013-08-14 | Ucl Business Plc | Anti-microbial agents and uses thereof |
| KR101949009B1 (ko) * | 2016-02-18 | 2019-02-18 | 경희대학교 산학협력단 | 융합단백질-다당 복합체의 제조방법 및 이의 용도 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4859377A (en) * | 1987-07-10 | 1989-08-22 | The United States Of America, As Represented By The Secretary Of Agriculture | Starch encapsulation of entomopathogens |
| US5137819A (en) * | 1988-07-08 | 1992-08-11 | University Of British Columbia | Cellulose binding fusion proteins for immobilization and purification of polypeptides |
| US5302523A (en) * | 1989-06-21 | 1994-04-12 | Zeneca Limited | Transformation of plant cells |
| US5349123A (en) * | 1990-12-21 | 1994-09-20 | Calgene, Inc. | Glycogen biosynthetic enzymes in plants |
| US5643756A (en) * | 1992-08-28 | 1997-07-01 | The Public Health Research Institute Of The City Of New York, Inc. | Fusion glycoproteins |
| GB9307408D0 (en) * | 1993-04-08 | 1993-06-02 | Danisco | Transgenic plants |
| US5512459A (en) * | 1993-07-20 | 1996-04-30 | Bionebraska, Inc. | Enzymatic method for modification or recombinant polypeptides |
| US5648244A (en) * | 1993-09-27 | 1997-07-15 | President And Fellows Of Harvard College | Production, purification, cleavage and use of fusion peptides |
| US5635599A (en) * | 1994-04-08 | 1997-06-03 | The United States Of America As Represented By The Department Of Health And Human Services | Fusion proteins comprising circularly permuted ligands |
| JP2001503964A (ja) * | 1996-05-29 | 2001-03-27 | ヘキスト シェリング アグレボ ゲーエムベーハー | デンプン合成に関与するコムギ由来酵素をコードする核酸分子 |
-
1997
- 1997-09-30 RU RU99108730/13A patent/RU2268301C9/ru not_active IP Right Cessation
- 1997-09-30 JP JP51677798A patent/JP2001505412A/ja not_active Ceased
- 1997-09-30 EP EP97910730A patent/EP0935665B1/en not_active Expired - Lifetime
- 1997-09-30 DE DE69738587T patent/DE69738587T2/de not_active Expired - Lifetime
- 1997-09-30 NZ NZ334637A patent/NZ334637A/en unknown
- 1997-09-30 WO PCT/US1997/017555 patent/WO1998014601A1/en not_active Ceased
- 1997-09-30 KR KR1019990702770A patent/KR20000048782A/ko not_active Ceased
- 1997-09-30 CN CNB971802327A patent/CN1195861C/zh not_active Expired - Fee Related
- 1997-09-30 AU AU48030/97A patent/AU730427B2/en not_active Ceased
- 1997-09-30 US US08/941,445 patent/US6107060A/en not_active Expired - Lifetime
- 1997-09-30 CA CA002265514A patent/CA2265514A1/en not_active Abandoned
- 1997-09-30 AT AT97910730T patent/ATE389725T1/de active
- 1997-09-30 BR BR9713242-0A patent/BR9713242A/pt not_active Application Discontinuation
- 1997-09-30 IL IL12915897A patent/IL129158A0/xx unknown
-
2003
- 2003-07-28 US US10/628,525 patent/US7141659B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2001505412A (ja) | 2001-04-24 |
| US6107060A (en) | 2000-08-22 |
| CA2265514A1 (en) | 1998-04-09 |
| KR20000048782A (ko) | 2000-07-25 |
| DE69738587D1 (de) | 2008-04-30 |
| US7141659B2 (en) | 2006-11-28 |
| EP0935665A1 (en) | 1999-08-18 |
| CN1195861C (zh) | 2005-04-06 |
| WO1998014601A1 (en) | 1998-04-09 |
| RU2268301C9 (ru) | 2006-07-20 |
| CN1239514A (zh) | 1999-12-22 |
| AU730427B2 (en) | 2001-03-08 |
| EP0935665B1 (en) | 2008-03-19 |
| US20040185114A1 (en) | 2004-09-23 |
| NZ334637A (en) | 2001-02-23 |
| RU2268301C2 (ru) | 2006-01-20 |
| IL129158A0 (en) | 2000-02-17 |
| AU4803097A (en) | 1998-04-24 |
| BR9713242A (pt) | 2000-01-18 |
| ATE389725T1 (de) | 2008-04-15 |
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