DE69736588D1 - Subtilisin Dimer mit verbesserter Aktivität und verfahren zur Herstellung davon. - Google Patents

Subtilisin Dimer mit verbesserter Aktivität und verfahren zur Herstellung davon.

Info

Publication number
DE69736588D1
DE69736588D1 DE69736588T DE69736588T DE69736588D1 DE 69736588 D1 DE69736588 D1 DE 69736588D1 DE 69736588 T DE69736588 T DE 69736588T DE 69736588 T DE69736588 T DE 69736588T DE 69736588 D1 DE69736588 D1 DE 69736588D1
Authority
DE
Germany
Prior art keywords
monomer unit
subtilisin
dimer
preparation
improved activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69736588T
Other languages
English (en)
Other versions
DE69736588T2 (de
Inventor
R Bott
Christian Paech
Shan Wu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Publication of DE69736588D1 publication Critical patent/DE69736588D1/de
Application granted granted Critical
Publication of DE69736588T2 publication Critical patent/DE69736588T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Textile Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Paper (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
DE69736588T 1996-03-29 1997-02-17 Subtilisin Dimer mit verbesserter Aktivität und verfahren zur Herstellung davon. Expired - Lifetime DE69736588T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/625,487 US6946280B1 (en) 1996-03-29 1996-03-29 Enzyme multimer and process of producing same
US625487 1996-03-29
PCT/US1997/002526 WO1997037007A1 (en) 1996-03-29 1997-02-17 Enzyme multimer and process of producing same

Publications (2)

Publication Number Publication Date
DE69736588D1 true DE69736588D1 (de) 2006-10-12
DE69736588T2 DE69736588T2 (de) 2007-09-27

Family

ID=24506318

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69736588T Expired - Lifetime DE69736588T2 (de) 1996-03-29 1997-02-17 Subtilisin Dimer mit verbesserter Aktivität und verfahren zur Herstellung davon.

Country Status (13)

Country Link
US (3) US6946280B1 (de)
EP (1) EP0889957B1 (de)
JP (1) JP2000507452A (de)
CN (1) CN1215429A (de)
AT (1) ATE338112T1 (de)
AU (1) AU719263B2 (de)
BR (1) BR9708453A (de)
CA (1) CA2248649C (de)
DE (1) DE69736588T2 (de)
DK (1) DK0889957T3 (de)
ES (1) ES2267132T3 (de)
NZ (1) NZ332236A (de)
WO (1) WO1997037007A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6946280B1 (en) * 1996-03-29 2005-09-20 Genencor International, Inc. Enzyme multimer and process of producing same
JP2002507426A (ja) 1998-03-26 2002-03-12 ザ、プロクター、エンド、ギャンブル、カンパニー アミノ酸置換を有するセリンプロテアーゼ変異体
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
DE69939538D1 (de) 1998-07-02 2008-10-23 Univ Toronto Mit einer kohlenhydratgruppe chemisch modifizierte proteine
JP4932990B2 (ja) 1998-11-10 2012-05-16 ダニスコ・ユーエス・インコーポレイテッド 化学修飾突然変異体セリンヒドロラーゼは改良された触媒活性およびキラル選択性を示す
US6379942B1 (en) 1998-12-21 2002-04-30 Genencor International, Inc. Chemically modified enzymes with multiple charged variants
AU781451B2 (en) 1999-04-28 2005-05-26 Genencor International, Inc. Specifically targeted catalytic antagonists and uses thereof
US6627744B2 (en) 1999-07-02 2003-09-30 Genencor International, Inc. Synthesis of glycodendrimer reagents
AU6085800A (en) 1999-07-22 2001-02-13 Procter & Gamble Company, The Subtilisin protease variants having amino acid substitutions in defined epitope regions
KR20020021397A (ko) 1999-07-22 2002-03-20 데이비드 엠 모이어 규정된 에피토프 영역에 아미노산 결실 및 치환을 갖는서브틸리신 프로테아제 변이체
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
MXPA02000837A (es) * 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen regiones epitopes protegidas estericamente.
TW593841B (en) * 2003-03-06 2004-06-21 Yuen Foong Yu Paper Mfg Co Ltd A plant nutrition formulated by recovery filtrate from plant fiber biopulping and method thereof

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0155832A3 (de) * 1984-03-16 1987-08-19 Genentech, Inc. Stabile Proteine, Verfahren zu deren Herstellung, dieselben kodierende Plasmide und andere DNS, diese Plasmide enthaltende Zellkulturen
US5185258A (en) * 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
US4844897A (en) * 1985-09-13 1989-07-04 Hiroshi Maeda Anti-tumor protease preparations
US4908773A (en) * 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
US4853871A (en) * 1987-04-06 1989-08-01 Genex Corporation Computer-based method for designing stablized proteins
EP0309746A1 (de) * 1987-09-08 1989-04-05 F. Hoffmann-La Roche Ag Antimalaria-Vakzine
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
JPH06207941A (ja) * 1992-05-29 1994-07-26 Toray Ind Inc 非ａ非ｂ型肝炎の検査方法
DK132892D0 (da) * 1992-10-30 1992-10-30 Novo Nordisk As Proteiner
US5795761A (en) * 1996-01-11 1998-08-18 Rutgers, The State University Of New Jersey Mutants of 2,5-diketo-D-gluconic acid (2,5-DKG) reductase A
US6946280B1 (en) * 1996-03-29 2005-09-20 Genencor International, Inc. Enzyme multimer and process of producing same

Also Published As

Publication number Publication date
BR9708453A (pt) 1999-08-03
ES2267132T3 (es) 2007-03-01
US7329528B2 (en) 2008-02-12
JP2000507452A (ja) 2000-06-20
NZ332236A (en) 2000-03-27
CA2248649A1 (en) 1997-10-09
US6946280B1 (en) 2005-09-20
ATE338112T1 (de) 2006-09-15
DE69736588T2 (de) 2007-09-27
AU2276997A (en) 1997-10-22
CA2248649C (en) 2010-10-12
US20050170471A1 (en) 2005-08-04
EP0889957A1 (de) 1999-01-13
US20060084160A1 (en) 2006-04-20
DK0889957T3 (da) 2007-01-08
WO1997037007A1 (en) 1997-10-09
CN1215429A (zh) 1999-04-28
EP0889957B1 (de) 2006-08-30
AU719263B2 (en) 2000-05-04

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Legal Events

Date Code Title Description
8381 Inventor (new situation)

Inventor name: BOTT, RICHARD, PALO ALTO, CA 94304-1013, US

Inventor name: PAECH, CHRISTIAN GENENCOR INTERNATIONAL, PALO , US

Inventor name: WU, SHAN GENENCOR INTERNATIONAL, PALO ALTO, CA, US

8364 No opposition during term of opposition