DE69719497T2 - Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung - Google Patents
Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösungInfo
- Publication number
- DE69719497T2 DE69719497T2 DE69719497T DE69719497T DE69719497T2 DE 69719497 T2 DE69719497 T2 DE 69719497T2 DE 69719497 T DE69719497 T DE 69719497T DE 69719497 T DE69719497 T DE 69719497T DE 69719497 T2 DE69719497 T2 DE 69719497T2
- Authority
- DE
- Germany
- Prior art keywords
- protein
- crystallization
- solution
- containing solution
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- RFAZFSACZIVZDV-UHFFFAOYSA-N butan-2-one Chemical compound CCC(C)=O.CCC(C)=O RFAZFSACZIVZDV-UHFFFAOYSA-N 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- FYGDTMLNYKFZSV-ZWSAEMDYSA-N cellotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](OC(O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-ZWSAEMDYSA-N 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 150000002009 diols Chemical class 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 230000001516 effect on protein Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- CAPAZTWTGPAFQE-UHFFFAOYSA-N ethane-1,2-diol Chemical compound OCCO.OCCO CAPAZTWTGPAFQE-UHFFFAOYSA-N 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000002334 glycols Chemical class 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229940035429 isobutyl alcohol Drugs 0.000 description 1
- 230000002366 lipolytic effect Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 108010003855 mesentericopeptidase Proteins 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- AHLBNYSZXLDEJQ-FWEHEUNISA-N orlistat Chemical compound CCCCCCCCCCC[C@H](OC(=O)[C@H](CC(C)C)NC=O)C[C@@H]1OC(=O)[C@H]1CCCCCC AHLBNYSZXLDEJQ-FWEHEUNISA-N 0.000 description 1
- QBHZMTFQTRUFIN-UHFFFAOYSA-N pentan-3-one Chemical compound CCC(=O)CC.CCC(=O)CC QBHZMTFQTRUFIN-UHFFFAOYSA-N 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 229940068886 polyethylene glycol 300 Drugs 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 229920001451 polypropylene glycol Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- YGSFNCRAZOCNDJ-UHFFFAOYSA-N propan-2-one Chemical compound CC(C)=O.CC(C)=O YGSFNCRAZOCNDJ-UHFFFAOYSA-N 0.000 description 1
- OJTDGPLHRSZIAV-UHFFFAOYSA-N propane-1,2-diol Chemical compound CC(O)CO.CC(O)CO OJTDGPLHRSZIAV-UHFFFAOYSA-N 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000001223 reverse osmosis Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000011877 solvent mixture Substances 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/816—Enzyme separation or purification by solubility
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK30596 | 1996-03-15 | ||
| PCT/DK1997/000113 WO1997034919A1 (en) | 1996-03-15 | 1997-03-14 | Method for purification of a protein from a protein containing solution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69719497D1 DE69719497D1 (de) | 2003-04-10 |
| DE69719497T2 true DE69719497T2 (de) | 2003-10-30 |
Family
ID=8092063
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69719497T Expired - Lifetime DE69719497T2 (de) | 1996-03-15 | 1997-03-14 | Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6087148A (https=) |
| EP (1) | EP0894091B1 (https=) |
| JP (1) | JP2000506534A (https=) |
| CN (1) | CN1216899C (https=) |
| AT (1) | ATE233784T1 (https=) |
| AU (1) | AU2151997A (https=) |
| DE (1) | DE69719497T2 (https=) |
| DK (1) | DK0894091T3 (https=) |
| IN (1) | IN190120B (https=) |
| WO (1) | WO1997034919A1 (https=) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997034919A1 (en) * | 1996-03-15 | 1997-09-25 | Novo Nordisk A/S | Method for purification of a protein from a protein containing solution |
| AU757991B2 (en) * | 1998-04-27 | 2003-03-13 | Altus Pharmaceuticals, Inc. | Stabilized protein crystals, formulations containing them and methods of making them |
| DE19859876A1 (de) * | 1998-12-23 | 2000-06-29 | Fraunhofer Ges Forschung | Verfahren zur Kristallisation aus Lösungen |
| GB9910975D0 (en) * | 1999-05-13 | 1999-07-14 | Univ Strathclyde | Rapid dehydration of proteins |
| US6663602B2 (en) | 2000-06-16 | 2003-12-16 | Novo Nordisk A/S | Injection device |
| JP4401775B2 (ja) | 2001-10-25 | 2010-01-20 | 協和発酵バイオ株式会社 | 酸化型グルタチオンの結晶およびその製造方法 |
| CA2504134C (en) * | 2002-11-01 | 2012-03-06 | Bayer Healthcare Llc | Process for concentration of macromolecules |
| US7686786B2 (en) | 2004-10-21 | 2010-03-30 | Novo Nordiks A/S | Dial-down mechanism for wind-up pen |
| EP2004671B1 (en) * | 2006-03-30 | 2015-12-09 | Novo Nordisk A/S | Two-phase precipitation of proteins |
| ATE458517T1 (de) | 2006-05-16 | 2010-03-15 | Novo Nordisk As | Getriebemechanismus für ein injektionsgerät |
| PL2023982T3 (pl) | 2006-05-18 | 2012-11-30 | Novo Nordisk As | Urządzenie wstrzykujące ze środkami blokującymi |
| DE102008029401A1 (de) * | 2008-06-23 | 2009-12-24 | Bayer Technology Services Gmbh | Verfahren zur Kristallisation von Peptiden und Proteinen |
| FR2943686B1 (fr) * | 2009-03-30 | 2013-11-01 | Roquette Freres | Procede d'obtention d'une preparation de beta-amylases a partir des fractions solubles de plantes amidonnieres |
| CN103998077B (zh) | 2011-12-29 | 2017-05-31 | 诺沃—诺迪斯克有限公司 | 具有向上拨动/向下拨动定量机构的基于扭力弹簧的卷紧自动注射器笔 |
| WO2023200284A1 (ko) * | 2022-04-15 | 2023-10-19 | 재단법인대구경북과학기술원 | 생체 시료 내 지질 연관 단백질의 질량 분석을 위한 지질 연관 단백질의 정제 및 농축 방법 |
| EP4568983A1 (en) * | 2022-08-10 | 2025-06-18 | Arla Foods amba | Crystallisation of a protein capable of crystallizing under salting-in conditions using multiple protein feeds |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3664926A (en) * | 1970-02-26 | 1972-05-23 | Merck & Co Inc | Crystallization of l-asparaginase |
| DE4001238A1 (de) * | 1990-01-18 | 1991-07-25 | Basf Ag | Verfahren zur anreicherung bzw. reinigung von biomolekuelen |
| DK115890D0 (da) * | 1990-05-09 | 1990-05-09 | Novo Nordisk As | Enzym |
| DK83093D0 (da) * | 1993-07-09 | 1993-07-09 | Novo Nordisk As | Fremgangsmaade |
| WO1997034919A1 (en) * | 1996-03-15 | 1997-09-25 | Novo Nordisk A/S | Method for purification of a protein from a protein containing solution |
-
1997
- 1997-03-14 WO PCT/DK1997/000113 patent/WO1997034919A1/en not_active Ceased
- 1997-03-14 JP JP9533055A patent/JP2000506534A/ja active Pending
- 1997-03-14 EP EP97914167A patent/EP0894091B1/en not_active Expired - Lifetime
- 1997-03-14 AU AU21519/97A patent/AU2151997A/en not_active Abandoned
- 1997-03-14 AT AT97914167T patent/ATE233784T1/de not_active IP Right Cessation
- 1997-03-14 DK DK97914167T patent/DK0894091T3/da active
- 1997-03-14 DE DE69719497T patent/DE69719497T2/de not_active Expired - Lifetime
- 1997-03-14 CN CN971930430A patent/CN1216899C/zh not_active Expired - Lifetime
- 1997-03-17 IN IN552MA1997 patent/IN190120B/en unknown
-
1998
- 1998-08-28 US US09/143,206 patent/US6087148A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CN1213377A (zh) | 1999-04-07 |
| ATE233784T1 (de) | 2003-03-15 |
| DE69719497D1 (de) | 2003-04-10 |
| EP0894091B1 (en) | 2003-03-05 |
| WO1997034919A1 (en) | 1997-09-25 |
| DK0894091T3 (da) | 2003-06-10 |
| AU2151997A (en) | 1997-10-10 |
| CN1216899C (zh) | 2005-08-31 |
| EP0894091A1 (en) | 1999-02-03 |
| US6087148A (en) | 2000-07-11 |
| JP2000506534A (ja) | 2000-05-30 |
| IN190120B (https=) | 2003-06-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |