DE69612575D1 - Hocheffiziente Übersetzung von mRNA Molukülen - Google Patents

Hocheffiziente Übersetzung von mRNA Molukülen

Info

Publication number
DE69612575D1
DE69612575D1 DE69612575T DE69612575T DE69612575D1 DE 69612575 D1 DE69612575 D1 DE 69612575D1 DE 69612575 T DE69612575 T DE 69612575T DE 69612575 T DE69612575 T DE 69612575T DE 69612575 D1 DE69612575 D1 DE 69612575D1
Authority
DE
Germany
Prior art keywords
highly efficient
mrna molecules
efficient translation
translation
mrna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69612575T
Other languages
English (en)
Other versions
DE69612575T2 (de
Inventor
David W Andrews
Akaterini Vassilakos
Martin John Glenton Hughes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
McMaster University
Original Assignee
McMaster University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by McMaster University filed Critical McMaster University
Application granted granted Critical
Publication of DE69612575D1 publication Critical patent/DE69612575D1/de
Publication of DE69612575T2 publication Critical patent/DE69612575T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69612575T 1995-02-10 1996-02-12 Hocheffiziente Übersetzung von mRNA Molukülen Expired - Fee Related DE69612575T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/386,921 US5824497A (en) 1995-02-10 1995-02-10 High efficiency translation of mRNA molecules

Publications (2)

Publication Number Publication Date
DE69612575D1 true DE69612575D1 (de) 2001-05-31
DE69612575T2 DE69612575T2 (de) 2001-10-18

Family

ID=23527635

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69612575T Expired - Fee Related DE69612575T2 (de) 1995-02-10 1996-02-12 Hocheffiziente Übersetzung von mRNA Molukülen

Country Status (4)

Country Link
US (2) US5824497A (de)
EP (1) EP0726319B1 (de)
CA (1) CA2169317A1 (de)
DE (1) DE69612575T2 (de)

Families Citing this family (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1892299A3 (de) * 1997-09-19 2011-07-20 Life Technologies Corporation Sense mRNA therapie
WO1999014346A2 (en) * 1997-09-19 1999-03-25 Sequitur, Inc. SENSE mRNA THERAPY
CA2396058C (en) * 1999-12-28 2009-09-15 Ribonomics, Inc. Methods for isolating and characterizing endogenous mrna-protein (mrnp) complexes
US8815517B2 (en) * 1999-12-28 2014-08-26 Ribonomics, Inc. Methods for identifying functionally related genes and drug targets
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
EP1470148B1 (de) 2002-02-01 2012-07-18 Life Technologies Corporation Doppelsträngige oligonukleotide
EP1572902B1 (de) 2002-02-01 2014-06-11 Life Technologies Corporation HOCHWIRKSAME siRNAS ZUR REDUZIERUNG DER EXPRESSION VON ZIELGENEN
AU2002953015A0 (en) * 2002-12-02 2002-12-12 Women's And Children's Hospital Modified lacz gene
WO2004111243A1 (en) * 2003-06-19 2004-12-23 Carbozyme Oy Stabilisation of mrna transcripts
PL2578685T3 (pl) 2005-08-23 2020-01-31 The Trustees Of The University Of Pennsylvania Rna zawierający zmodyfikowane nukleozydy i sposoby jego zastosowania
US9012219B2 (en) 2005-08-23 2015-04-21 The Trustees Of The University Of Pennsylvania RNA preparations comprising purified modified RNA for reprogramming cells
DE102005046490A1 (de) 2005-09-28 2007-03-29 Johannes-Gutenberg-Universität Mainz Modifikationen von RNA, die zu einer erhöhten Transkriptstabilität und Translationseffizienz führen
WO2008097926A2 (en) * 2007-02-02 2008-08-14 Yale University Transient transfection with rna
US10155038B2 (en) 2007-02-02 2018-12-18 Yale University Cells prepared by transient transfection and methods of use thereof
US9249423B2 (en) 2007-02-02 2016-02-02 Yale University Method of de-differentiating and re-differentiating somatic cells using RNA
US10837020B2 (en) 2009-04-22 2020-11-17 Massachusetts Institute Of Technology Innate immune suppression enables repeated delivery of long RNA molecules
US20100273220A1 (en) * 2009-04-22 2010-10-28 Massachusetts Institute Of Technology Innate immune suppression enables repeated delivery of long rna molecules
KR102171849B1 (ko) * 2009-12-07 2020-10-30 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 세포 리프로그래밍을 위한 정제된 변형 ｒｎａ를 포함하는 ｒｎａ 제제
ES2696825T3 (es) 2009-12-10 2019-01-18 Univ Minnesota Modificación del ADN inducida por el efector TAL
CA2793959C (en) 2010-03-25 2019-06-04 Oregon Health & Science University Cmv glycoproteins and recombinant vectors
US8822663B2 (en) 2010-08-06 2014-09-02 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
EP2625189B1 (de) 2010-10-01 2018-06-27 ModernaTX, Inc. Manipulierte nukleinsäuren und anwendungsverfahren dafür
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
PL2691530T3 (pl) 2011-06-10 2019-02-28 Oregon Health & Science University Glikoproteiny i rekombinowane wektory CMV
EP2568289A3 (de) 2011-09-12 2013-04-03 International AIDS Vaccine Initiative Immunselektion von rekombinantem vesikulärem Stomatitisvirus mit Expression von HIV-1-Proteinen durch Breitbandneutralisierungs-Antikörper
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
HUE057725T2 (hu) * 2011-10-03 2022-06-28 Modernatx Inc Módosított nukleozidok, nukleotidok és nukleinsavak és ezek felhasználása
EP2586461A1 (de) 2011-10-27 2013-05-01 Christopher L. Parks Von einem eingehüllten Virus abgeleitete Virenpartikel
HRP20220717T1 (hr) 2011-12-16 2022-07-22 Modernatx, Inc. Modificirani pripravci mrna
SI3421601T1 (sl) 2011-12-30 2020-03-31 Cellscript, Llc Izdelava in uporaba in vitro-sintetizirane ssRNA za uvajanje v celice sesalca, da se inducira biološki ali biokemijski učinek
US10501512B2 (en) 2012-04-02 2019-12-10 Modernatx, Inc. Modified polynucleotides
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
AU2013243947A1 (en) 2012-04-02 2014-10-30 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
EP2679596B1 (de) 2012-06-27 2017-04-12 International Aids Vaccine Initiative HIV-1 Env-proteinvariante
MX2015004853A (es) * 2012-10-16 2015-12-09 Massachusetts Inst Technology Produccion de acidos ribonucleicos no poliadenilados estables.
ES2921623T3 (es) 2012-11-26 2022-08-30 Modernatx Inc ARN modificado terminalmente
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
AU2014265608B2 (en) 2013-05-15 2018-12-20 Robert Kruse Intracellular translation of circular RNA
US20150065381A1 (en) 2013-09-05 2015-03-05 International Aids Vaccine Initiative Methods of identifying novel hiv-1 immunogens
EP3052106A4 (de) 2013-09-30 2017-07-19 ModernaTX, Inc. Polynukleotide zur codierung immunmodulierender polypeptide
EP3052521A1 (de) 2013-10-03 2016-08-10 Moderna Therapeutics, Inc. Polynukleotide zur codierung von low-density-lipoprotein-rezeptor
US10058604B2 (en) 2013-10-07 2018-08-28 International Aids Vaccine Initiative Soluble HIV-1 envelope glycoprotein trimers
HRP20220070T1 (hr) 2014-04-23 2022-04-01 Modernatx, Inc. Cjepiva nukleinske kiseline
EP3069730A3 (de) 2015-03-20 2017-03-15 International Aids Vaccine Initiative Lösliche hiv-1-hüllglykoproteintrimere
EP3072901A1 (de) 2015-03-23 2016-09-28 International Aids Vaccine Initiative Lösliche hiv-1-hüllglykoproteintrimere
WO2017123242A1 (en) * 2016-01-15 2017-07-20 Ding Enyu A universal nucleic acid drug vector enhancing mrna stability and translatability
CN110430894A (zh) 2017-02-01 2019-11-08 莫得纳特斯公司 编码活化致癌基因突变肽的免疫调节治疗性mrna组合物
US11485972B2 (en) 2017-05-18 2022-11-01 Modernatx, Inc. Modified messenger RNA comprising functional RNA elements
US20200268666A1 (en) 2017-06-14 2020-08-27 Modernatx, Inc. Polynucleotides encoding coagulation factor viii
WO2019104160A2 (en) 2017-11-22 2019-05-31 Modernatx, Inc. Polynucleotides encoding phenylalanine hydroxylase for the treatment of phenylketonuria
CA3079543A1 (en) 2017-11-22 2019-05-31 Modernatx, Inc. Polynucleotides encoding propionyl-coa carboxylase alpha and beta subunits for the treatment of propionic acidemia
WO2019104152A1 (en) 2017-11-22 2019-05-31 Modernatx, Inc. Polynucleotides encoding ornithine transcarbamylase for the treatment of urea cycle disorders
EP3773745A1 (de) 2018-04-11 2021-02-17 ModernaTX, Inc. Messenger-rna mit funktionalen rna-elementen
WO2020023390A1 (en) 2018-07-25 2020-01-30 Modernatx, Inc. Mrna based enzyme replacement therapy combined with a pharmacological chaperone for the treatment of lysosomal storage disorders
MA53545A (fr) 2018-09-02 2021-07-14 Modernatx Inc Polynucléotides codant pour l'acyl-coa déshydrogénase à très longue chaîne pour le traitement de l'insuffisance en acyl-coa déshydrogénase à très longue chaîne
MA53609A (fr) 2018-09-13 2021-07-21 Modernatx Inc Polynucléotides codant la glucose-6-phosphatase pour le traitement de la glycogénose
WO2020056155A2 (en) 2018-09-13 2020-03-19 Modernatx, Inc. Polynucleotides encoding branched-chain alpha-ketoacid dehydrogenase complex e1-alpha, e1-beta, and e2 subunits for the treatment of maple syrup urine disease
CA3112398A1 (en) 2018-09-14 2020-03-19 Modernatx, Inc. Polynucleotides encoding uridine diphosphate glycosyltransferase 1 family, polypeptide a1 for the treatment of crigler-najjar syndrome
EP3856233A1 (de) 2018-09-27 2021-08-04 Modernatx, Inc. Arginase-1-codierende polynukleotide zur behandlung von arginasemangel
US20220226438A1 (en) 2019-05-08 2022-07-21 Astrazeneca Ab Compositions for skin and wounds and methods of use thereof
US20220387628A1 (en) 2019-06-24 2022-12-08 Modernatx, Inc. Messenger rna comprising functional rna elements and uses thereof
US20240123034A1 (en) 2019-10-15 2024-04-18 Modernatx, Inc. Mrnas encoding granulocyte-macrophage colony stimulating factor for treating parkinson's disease
EP4045527A1 (de) 2019-10-15 2022-08-24 ModernaTX, Inc. Für mrnas codierende immunmodulierende polynukleotide und verwendungen davon
WO2021107647A1 (ko) * 2019-11-26 2021-06-03 한국생명공학연구원 단백질 발현용 mrna 구조체 및 이의 용도
EP4087544A1 (de) 2020-01-10 2022-11-16 ModernaTX, Inc. Verfahren zur herstellung tolerogener dendritischer zellen
US20230130155A1 (en) 2020-01-30 2023-04-27 Modernatx, Inc. Mrnas encoding metabolic reprogramming polypeptides and uses thereof
CA3182920A1 (en) 2020-05-14 2021-11-18 Modernatx, Inc. Lnp compositions comprising an mrna therapeutic and an effector molecule
CA3184474A1 (en) 2020-06-01 2021-12-09 Modernatx, Inc. Phenylalanine hydroxylase variants and uses thereof
US20230242908A1 (en) 2020-06-23 2023-08-03 Modernatx, Inc. Lnp compositions comprising mrna therapeutics with extended half-life
WO2022204371A1 (en) 2021-03-24 2022-09-29 Modernatx, Inc. Lipid nanoparticles containing polynucleotides encoding glucose-6-phosphatase and uses thereof
WO2022204370A1 (en) 2021-03-24 2022-09-29 Modernatx, Inc. Lipid nanoparticles and polynucleotides encoding ornithine transcarbamylase for the treatment of ornithine transcarbamylase deficiency
WO2022204390A1 (en) 2021-03-24 2022-09-29 Modernatx, Inc. Lipid nanoparticles containing polynucleotides encoding phenylalanine hydroxylase and uses thereof
WO2022204369A1 (en) 2021-03-24 2022-09-29 Modernatx, Inc. Polynucleotides encoding methylmalonyl-coa mutase for the treatment of methylmalonic acidemia
WO2022204380A1 (en) 2021-03-24 2022-09-29 Modernatx, Inc. Lipid nanoparticles containing polynucleotides encoding propionyl-coa carboxylase alpha and beta subunits and uses thereof
KR20220159909A (ko) * 2021-05-25 2022-12-05 한국생명공학연구원 단백질 발현용 mRNA 제조방법 및 이에 의해 제조된 mRNA의 용도
WO2022271776A1 (en) 2021-06-22 2022-12-29 Modernatx, Inc. Polynucleotides encoding uridine diphosphate glycosyltransferase 1 family, polypeptide a1 for the treatment of crigler-najjar syndrome
WO2023056044A1 (en) 2021-10-01 2023-04-06 Modernatx, Inc. Polynucleotides encoding relaxin for the treatment of fibrosis and/or cardiovascular disease
WO2023196399A1 (en) 2022-04-06 2023-10-12 Modernatx, Inc. Lipid nanoparticles and polynucleotides encoding argininosuccinate lyase for the treatment of argininosuccinic aciduria
CN117165611A (zh) * 2022-05-27 2023-12-05 深圳市三源生生物科技有限公司 一种构建mRNA体外转录模板的骨架

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1209501A (en) * 1982-09-16 1986-08-12 Nikos Panayotatos Expression vector
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
US5079159A (en) * 1983-12-27 1992-01-07 Genetics Institute, Inc. Method for making tissue plasminogen activator
US5149635A (en) * 1986-09-12 1992-09-22 Abbott Biotech, Inc. Messenger RNA stabilization in animal cells
US5026639A (en) * 1988-01-14 1991-06-25 Nippon Mining Company, Limited Method to improve mRNA translation and use thereof for production of platelet factor-4
DK0426641T3 (da) * 1989-10-31 2000-10-23 Monsanto Co Promotor til transgene planter
US5593874A (en) * 1992-03-19 1997-01-14 Monsanto Company Enhanced expression in plants
US5362865A (en) * 1993-09-02 1994-11-08 Monsanto Company Enhanced expression in plants using non-translated leader sequences

Also Published As

Publication number Publication date
EP0726319B1 (de) 2001-04-25
EP0726319A2 (de) 1996-08-14
DE69612575T2 (de) 2001-10-18
EP0726319A3 (de) 1997-10-22
US5824497A (en) 1998-10-20
US5807707A (en) 1998-09-15
CA2169317A1 (en) 1996-08-11

Similar Documents

Publication Publication Date Title
DE69612575D1 (de) Hocheffiziente Übersetzung von mRNA Molukülen
DE69533361D1 (de) Umsetzung von kohlenwasserstoffen
DE69608448T2 (de) Reduktion des biobewuchses
PT988265E (pt) Processamento de aromaticos pesados
DE19882126T1 (de) Hydrierung von Kohlenwasserstoffen
DE69626729D1 (de) Kombination von Datenwerten
DE69628195D1 (de) Verwendung von xyloglucanendotransglycosylase
DE69632258D1 (de) Verminderung von schlechten geruchen
DE69519381T2 (de) Modifizierung von ölen
ATA136995A (de) Abreicherung von 7-adca in 3-vinyl-aca
DE59705708D1 (de) Lagebestimmung von peripherieeinheiten
DE69709159T2 (de) Kreuz-dimierisierung von olefinen
DE59607408D1 (de) Mischungen von Monoazopyridonfarbstoffen
DE69605708D1 (de) Reinigung von Pentafluorethan
DE29509987U1 (de) Bausatz von Steckverbindern
DE69515021D1 (de) Ausrichtung von Interferometern
DE69611224D1 (de) Verringerung von videomoire
DE59501871D1 (de) Monocarbonsäureamide von Polyaminen
DE29622383U1 (de) Verbindungsanordnung von Bauelementen
DE69804782D1 (de) Isomerisierung von fluorkohlenwasserstoffen
DE69713192T2 (de) Kombination von Enzymen
DE862581T1 (de) Heparinchromatographie von erythropoietinen
SE9502596D0 (sv) New DNA molecules II
KR960027486U (ko) 조명등의 회전구조
IS4339A (is) Hömlun ljóssundurliðunar á 3-setnum-2-oxindólum

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee