DE69511693D1 - Bestimmung der tetanus- und botulinum toxine - Google Patents

Bestimmung der tetanus- und botulinum toxine

Info

Publication number
DE69511693D1
DE69511693D1 DE69511693T DE69511693T DE69511693D1 DE 69511693 D1 DE69511693 D1 DE 69511693D1 DE 69511693 T DE69511693 T DE 69511693T DE 69511693 T DE69511693 T DE 69511693T DE 69511693 D1 DE69511693 D1 DE 69511693D1
Authority
DE
Germany
Prior art keywords
tetanus
toxin
determination
substrate
botulinum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69511693T
Other languages
English (en)
Other versions
DE69511693T2 (de
Inventor
Clifford Charles Shone
Bassam Hallis
Benjamin Arthur Frederic James
Conrad Padraig Quinn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syntaxin Ltd
Original Assignee
Microbiological Research Authority
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microbiological Research Authority filed Critical Microbiological Research Authority
Publication of DE69511693D1 publication Critical patent/DE69511693D1/de
Application granted granted Critical
Publication of DE69511693T2 publication Critical patent/DE69511693T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69511693T 1994-06-03 1995-06-02 Bestimmung der tetanus- und botulinum toxine Expired - Lifetime DE69511693T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9411138A GB9411138D0 (en) 1994-06-03 1994-06-03 Toxin assay
PCT/GB1995/001279 WO1995033850A1 (en) 1994-06-03 1995-06-02 Toxin assay

Publications (2)

Publication Number Publication Date
DE69511693D1 true DE69511693D1 (de) 1999-09-30
DE69511693T2 DE69511693T2 (de) 2000-03-09

Family

ID=10756143

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69511693T Expired - Lifetime DE69511693T2 (de) 1994-06-03 1995-06-02 Bestimmung der tetanus- und botulinum toxine

Country Status (9)

Country Link
EP (1) EP0763131B1 (de)
JP (1) JP4246259B2 (de)
AT (1) ATE183779T1 (de)
AU (1) AU687564B2 (de)
CA (1) CA2191895C (de)
DE (1) DE69511693T2 (de)
DK (1) DK0763131T3 (de)
GB (1) GB9411138D0 (de)
WO (1) WO1995033850A1 (de)

Families Citing this family (43)

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Publication number Priority date Publication date Assignee Title
WO1997034620A1 (en) * 1996-03-18 1997-09-25 The Regents Of The University Of California Peptide inhibitors of neurotransmitter secretion by neuronal cells
US7192596B2 (en) 1996-08-23 2007-03-20 The Health Protection Agency Ipsen Limited Recombinant toxin fragments
US5965699A (en) * 1996-11-06 1999-10-12 The United States Of America As Represented By The Secretary Of The Army Assay for the proteolytic activity of serotype a from clostridium botulinum
GB9721189D0 (en) 1997-10-08 1997-12-03 Speywood Lab The Limited Analgesic conjugates
US20040071736A1 (en) 1998-08-25 2004-04-15 Health Protection Agency Methods and compounds for the treatment of mucus hypersecretion
US8790897B2 (en) 1998-08-25 2014-07-29 Syntaxin Ltd. Treatment of mucus hypersecretion
GB9818548D0 (en) 1998-08-25 1998-10-21 Microbiological Res Authority Treatment of mucas hypersecretion
AU781608B2 (en) * 1999-05-14 2005-06-02 U.S. Army Medical Research And Materiel Command Buforin I as a specific inhibitor and therapeutic agent for botulinum toxin B and tetanus neurotoxins
US6143513A (en) * 1999-06-23 2000-11-07 Biacore Ab Method and kit for detecting betalactam-containing compounds
GB9922554D0 (en) * 1999-09-23 1999-11-24 Microbiological Res Authority Inhibition of secretion from non-neuronal cells
FR2809733B1 (fr) 2000-06-02 2004-04-30 Inst Nat Sante Rech Med Substrat peptidique reconnu par la toxine botulique de type bont/b et son utilisation pour doser et/ou detecter ladite toxine ou des inhibiteurs correspondants
WO2002025284A2 (en) * 2000-09-25 2002-03-28 U.S. Medical Research Institute Of Infectious Diseases High-throughput assays for the proteolytic activities of clostridial neurotoxins
US7208285B2 (en) 2001-08-28 2007-04-24 Allergan, Inc. Fret protease assays for botulinum serotype A/E toxins
US8022172B2 (en) 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US7374896B2 (en) 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
US7332567B2 (en) 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
US7183066B2 (en) 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
GB0311961D0 (en) 2003-05-23 2003-06-25 Health Prot Agency Mass-based toxin assay and substrates therefor
US7399607B2 (en) 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
GB0426394D0 (en) 2004-12-01 2005-01-05 Health Prot Agency Fusion proteins
DK2271670T3 (en) 2008-03-14 2014-12-01 Allergan Inc IMMUNE BASED ACTIVITY ASSAYS WITH BOTULINUM TOXIN SEROTYPE A
KR101604515B1 (ko) 2008-03-14 2016-03-17 알러간, 인코포레이티드 면역-기반 보툴리눔 독소 세로타입 ａ 활성 검정
FR2930447B1 (fr) 2008-04-25 2010-07-30 Sod Conseils Rech Applic Utilisation therapeutique d'au moins une neurotoxine botulique dans le traitement de la douleur dans le cas de la neuropathie diabetique
WO2010085473A1 (en) 2009-01-20 2010-07-29 Trustees Of Tufts College Methods for the delivery of toxins or enzymatically active portions thereof
AU2010223910B2 (en) 2009-03-13 2015-03-12 Allergan, Inc. Cells useful for immuno-based Botulinum toxin serotype A activity assays
PT3281953T (pt) * 2009-03-13 2020-01-15 Allergan Inc Ensaios de base imunológica da actividade de endopeptidase redireccionada
RU2545783C9 (ru) * 2009-04-27 2016-02-27 Мерц Фарма Гмбх Унд Ко. Кгаа Средства и способы определения количества полипептида нейротоксина и его каталитической и протеолитической активностей
US8129139B2 (en) 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
CA2780932A1 (en) * 2009-11-18 2011-05-26 Merz Pharma Gmbh & Co. Kgaa Assay for quantifying clostridial neurotoxin
MX348188B (es) 2011-09-29 2017-06-01 Cellsnap Llc Composiciones y metodos para pruebas de toxigenicidad.
EP2798077A2 (de) 2011-12-31 2014-11-05 Allergan, Inc. Hochempfindlicher zellbasierter test für den nachweis der anwesenheit von aktivem botulinum-neurotoxin-serotyp-a
PT3372239T (pt) 2012-05-30 2021-02-02 Harvard College Neurotoxina botulínica geneticamente modificada
GB201219024D0 (en) 2012-10-23 2012-12-05 Syntaxin Ltd Assay
CA2885294A1 (en) 2012-11-21 2014-05-30 Merz Pharma Gmbh & Co. Kgaa Means and methods for determination of botulinum neurotoxin biological activity
AU2014301116B2 (en) 2013-06-28 2019-11-14 Merz Pharma Gmbh & Co. Kgaa Means and methods for the determination of the biological activity of Neurotoxin polypeptides in cells
KR20170026624A (ko) 2014-07-07 2017-03-08 알러간, 인코포레이티드 조직 샘플에서 절단된 snap25를 검출하는 방법
EP3233911B1 (de) 2014-12-19 2020-10-14 Merz Pharma GmbH & Co. KGaA Mittel und verfahren zur bestimmung der biologischen aktivität von bont/e in zellen
PL3274364T3 (pl) 2015-03-26 2022-01-10 President And Fellows Of Harvard College Neurotoksyna botulinowa uzyskana technikami inżynierii
CN109069596A (zh) 2016-03-25 2018-12-21 益普生生物制药有限公司 收集增强肉毒杆菌毒素的治疗效果的物理疗法信息
UA127310C2 (uk) 2016-05-16 2023-07-19 Презідент Енд Феллоуз Оф Гарвард Колледж Спосіб очищення і активації ботулінічного нейротоксину
WO2018039506A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Engineered botulinum neurotoxin
JP7217700B2 (ja) 2016-09-13 2023-02-03 アラーガン、インコーポレイテッド 安定化非タンパク質クロストリジウム毒素組成物
EP3312290A1 (de) 2016-10-18 2018-04-25 Ipsen Biopharm Limited Zellulärer vamp-spaltungstest

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5171662A (en) * 1990-09-13 1992-12-15 The Upjohn Company Method of detecting HIV protease activity
EP0529719A1 (de) * 1991-08-26 1993-03-03 Merck & Co. Inc. Test zur Bestimmung der Hemmung der PMN-Elastase durch N-substituierte Azetidinone

Also Published As

Publication number Publication date
AU687564B2 (en) 1998-02-26
EP0763131B1 (de) 1999-08-25
JP4246259B2 (ja) 2009-04-02
CA2191895C (en) 2009-03-17
DK0763131T3 (da) 1999-12-13
CA2191895A1 (en) 1995-12-14
DE69511693T2 (de) 2000-03-09
JPH10504801A (ja) 1998-05-12
ATE183779T1 (de) 1999-09-15
EP0763131A1 (de) 1997-03-19
WO1995033850A1 (en) 1995-12-14
GB9411138D0 (en) 1994-07-27
AU2624095A (en) 1996-01-04

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: HEALTH PROTECTION AGENCY, SALISBURY, WILTSHIRE, GB

8327 Change in the person/name/address of the patent owner

Owner name: SYNTAXIN LTD., ABINGDON, OXFORDSHIRE, GB