DE69424151T2 - Automatisierte synthese von oligonukleotiden - Google Patents

Automatisierte synthese von oligonukleotiden

Info

Publication number
DE69424151T2
DE69424151T2 DE69424151T DE69424151T DE69424151T2 DE 69424151 T2 DE69424151 T2 DE 69424151T2 DE 69424151 T DE69424151 T DE 69424151T DE 69424151 T DE69424151 T DE 69424151T DE 69424151 T2 DE69424151 T2 DE 69424151T2
Authority
DE
Germany
Prior art keywords
oligonucleotides
automated synthesis
automated
synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69424151T
Other languages
English (en)
Other versions
DE69424151D1 (de
Inventor
A Mcgraw
Michael Grosse
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Georgia Research Foundation Inc UGARF
Original Assignee
University of Georgia Research Foundation Inc UGARF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Georgia Research Foundation Inc UGARF filed Critical University of Georgia Research Foundation Inc UGARF
Publication of DE69424151D1 publication Critical patent/DE69424151D1/de
Application granted granted Critical
Publication of DE69424151T2 publication Critical patent/DE69424151T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00286—Reactor vessels with top and bottom openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00308—Reactor vessels in a multiple arrangement interchangeably mounted in racks or blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00414—Means for dispensing and evacuation of reagents using suction
    • B01J2219/00416—Vacuum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00418—Means for dispensing and evacuation of reagents using pressure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00423—Means for dispensing and evacuation of reagents using filtration, e.g. through porous frits
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00689—Automatic using computers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00695—Synthesis control routines, e.g. using computer programs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE69424151T 1993-02-11 1994-02-04 Automatisierte synthese von oligonukleotiden Expired - Lifetime DE69424151T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/016,739 US5368823A (en) 1993-02-11 1993-02-11 Automated synthesis of oligonucleotides
PCT/US1994/001271 WO1994018226A1 (en) 1993-02-11 1994-02-04 Automated synthesis of oligonucleotides

Publications (2)

Publication Number Publication Date
DE69424151D1 DE69424151D1 (de) 2000-05-31
DE69424151T2 true DE69424151T2 (de) 2000-09-21

Family

ID=21778699

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69424151T Expired - Lifetime DE69424151T2 (de) 1993-02-11 1994-02-04 Automatisierte synthese von oligonukleotiden

Country Status (5)

Country Link
US (2) US5368823A (de)
EP (1) EP0683790B1 (de)
JP (1) JPH08506813A (de)
DE (1) DE69424151T2 (de)
WO (1) WO1994018226A1 (de)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1382386A3 (de) * 1992-02-19 2004-12-01 The Public Health Research Institute Of The City Of New York, Inc. Neue Ordnungen für Oligonukleotide und ihr Nutzen zum Sortieren, Isolieren, Sequenzierung und Manipulieren von Nukleinsäuren
US5472672A (en) * 1993-10-22 1995-12-05 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and method for polymer synthesis using arrays
US6558633B1 (en) * 1994-09-21 2003-05-06 Isis Pharmaceuticals, Inc. Chemical reaction apparatus and methods
US5585069A (en) * 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
US5614608A (en) * 1995-01-20 1997-03-25 Selectide Corporation Apparatus and method for multiple synthesis of organic compounds on polymer support
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
US5641459A (en) * 1995-08-17 1997-06-24 Pharmacia Biotech Ab Machine for synthesizing oligonucleotides
US5888830A (en) * 1995-09-22 1999-03-30 Berlex Laboratories, Inc. Apparatus and process for multiple chemical reactions
AU723605B2 (en) * 1995-09-22 2000-08-31 Berlex Laboratories, Inc. Apparatus and process for multiple chemical reactions
CA2198433A1 (en) * 1996-03-22 1997-09-23 Sheila Helen Hobbs Dewitt Information management system for automated multiple simultaneous synthesis
US6706875B1 (en) * 1996-04-17 2004-03-16 Affyemtrix, Inc. Substrate preparation process
US5733509A (en) * 1996-04-17 1998-03-31 Motorola, Inc. Method and system for synthesizing oligonucleotides using nucleotide-specific dispensing bars
US5792430A (en) * 1996-08-12 1998-08-11 Monsanto Company Solid phase organic synthesis device with pressure-regulated manifold
WO1998006490A1 (en) * 1996-08-12 1998-02-19 Monsanto Company Solid phase organic synthesis device with pressure-regulated manifold
US6274094B1 (en) 1997-01-13 2001-08-14 Weller, Iii Harold Norris Nestable, modular apparatus for synthesis of multiple organic compounds
US6518067B1 (en) * 1997-02-17 2003-02-11 Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf) Automated chemical synthesis apparatus
US6126904A (en) * 1997-03-07 2000-10-03 Argonaut Technologies, Inc. Apparatus and methods for the preparation of chemical compounds
US5961925A (en) * 1997-09-22 1999-10-05 Bristol-Myers Squibb Company Apparatus for synthesis of multiple organic compounds with pinch valve block
CA2253164A1 (en) * 1997-11-14 1999-05-14 Edward Michael Sioma Apparatus and method used in multiple, simultaneous synthesis of general compounds
US6270730B1 (en) * 1998-06-16 2001-08-07 Northwest Engineering Inc. Multi-well rotary synthesizer
US6306658B1 (en) * 1998-08-13 2001-10-23 Symyx Technologies Parallel reactor with internal sensing
US6548026B1 (en) 1998-08-13 2003-04-15 Symyx Technologies, Inc. Parallel reactor with internal sensing and method of using same
US6890492B1 (en) 1998-08-13 2005-05-10 Symyx Technologies, Inc. Parallel reactor with internal sensing and method of using same
US6528026B2 (en) 1998-08-13 2003-03-04 Symyx Technologies, Inc. Multi-temperature modular reactor and method of using same
US6455316B1 (en) 1998-08-13 2002-09-24 Symyx Technologies, Inc. Parallel reactor with internal sensing and method of using same
DE19940751A1 (de) 1998-08-28 2000-03-02 Febit Ferrarius Biotech Gmbh Lichtemissions-Detektionseinrichtung
JP4569850B2 (ja) * 1998-09-24 2010-10-27 株式会社島津製作所 自動合成機
US6258323B1 (en) 1998-11-16 2001-07-10 Rohm And Haas Company Apparatus and method used in multiple, simultaneous synthesis of general compounds
US6309828B1 (en) 1998-11-18 2001-10-30 Agilent Technologies, Inc. Method and apparatus for fabricating replicate arrays of nucleic acid molecules
US6264891B1 (en) * 1998-12-22 2001-07-24 Eos Biotechnology, Inc. Apparatus and method for concurrent chemical synthesis
JP2002536158A (ja) * 1999-02-05 2002-10-29 インヴィトロゲン・コーポレーション 流体またはガスの自動送達のためのシステム、方法およびコンピュータプログラム製品
EP1153127B1 (de) 1999-02-19 2006-07-26 febit biotech GmbH Verfahren zur herstellung von polymeren
US6800250B1 (en) 1999-03-19 2004-10-05 Scott P. Hunicke-Smith Polymer synthesizer
AU4011800A (en) * 1999-03-19 2000-10-09 Genomic Instrumentation Services, Inc. Polymer synthesizer
US6143252A (en) * 1999-04-12 2000-11-07 The Perkin-Elmer Corporation Pipetting device with pipette tip for solid phase reactions
DE19919607A1 (de) * 1999-04-29 2000-11-16 Mets Ion Gmbh Ges Fuer Angewan Verfahren und Vorrichtung zur parallelen Herstellung von wenigstens 4n Oligonukleotiden
EP1296756B1 (de) * 2000-03-28 2006-09-27 Laser- und Medizin-Technologie GGmbH Berlin Verfahren und vorrichtung zur untersuchung der molekularen wechselwirkungen von löslichen oder suspendierbaren wirkstoffen mit festphasen-gebundenen peptidischen oder peptoiden zielmolekülen durch kapillarplatten mit grosser innerer oberfläche
US6994827B2 (en) * 2000-06-03 2006-02-07 Symyx Technologies, Inc. Parallel semicontinuous or continuous reactors
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20030113236A1 (en) * 2001-08-15 2003-06-19 Third Wave Technologies, Inc. Polymer synthesizer
US20030072689A1 (en) * 2001-08-15 2003-04-17 Third Wave Technologies, Inc. Polymer synthesizer
US7435390B2 (en) * 2001-01-26 2008-10-14 Third Wave Technologies, Inc. Nucleic acid synthesizers
US6932943B1 (en) 2001-01-26 2005-08-23 Third Wave Technologies Nucleic acid synthesizers
US20080261220A1 (en) * 2000-11-30 2008-10-23 Third Wave Technologies, Inc. Nucleic Acid Detection Assays
US6867050B2 (en) * 2001-05-16 2005-03-15 Academia Sinica Apparatus and methods for chemical synthesis
US20030086829A1 (en) * 2001-10-24 2003-05-08 Livesay Eric A. High throughput chemical synthesizer
US20030098271A1 (en) * 2001-11-26 2003-05-29 Ralph Somack Capsule and tray systems for combined sample collection, archiving, purification, and PCR
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US20040253620A1 (en) * 2003-04-30 2004-12-16 Invitrogen Corporation Use of site specific recombination to prepare molecular markers
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
WO2005058471A2 (en) * 2003-12-15 2005-06-30 Applera Corporation Automated oligomer synthesis
US20070050092A1 (en) * 2005-08-12 2007-03-01 Symyx Technologies, Inc. Event-based library process design
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US8211370B2 (en) 2007-12-07 2012-07-03 Thomas Downing Polymer synthesizer
WO2009092564A2 (en) * 2008-01-23 2009-07-30 Roche Diagnostics Gmbh Integrated instrument performing synthesis and amplification
KR101418355B1 (ko) * 2009-10-23 2014-07-11 (주)바이오니아 고밀도 유전자 합성기
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
KR20110092046A (ko) * 2010-02-08 2011-08-17 삼성전자주식회사 모세관 노즐을 포함하는 마이크로어레이 프로브 합성 장치 및 이를 이용한 마이크로어레이 프로브 합성 방법
US8580201B2 (en) * 2010-02-22 2013-11-12 Oligoco, Inc. Wicking-based reagent-solution draining in an automated system
EP3360963B1 (de) 2010-11-12 2019-11-06 Gen9, Inc. Verfahren und vorrichtungen für nukleinsäuresynthese
EP2637780B1 (de) 2010-11-12 2022-02-09 Gen9, Inc. Proteinanordnungen und verfahren zu ihrer herstellung und verwendung
LT3594340T (lt) 2011-08-26 2021-10-25 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP2841601B1 (de) 2012-04-24 2019-03-06 Gen9, Inc. Verfahren zum sortieren von nukleinsäuren und zur multiplexierten, vorbereitenden in-vitro-klonung
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
SG11201500724QA (en) * 2012-08-16 2015-02-27 Synthetic Genomics Inc Digital to biological converter
US9069358B2 (en) 2013-06-24 2015-06-30 Biolytic Lab Performance, Inc. System for controlling and optimizing reactions in solid phase synthesis of small molecules
TWI646230B (zh) 2013-08-05 2019-01-01 扭轉生物科技有限公司 重新合成之基因庫
US10040048B1 (en) 2014-09-25 2018-08-07 Synthego Corporation Automated modular system and method for production of biopolymers
CN105733936B (zh) * 2014-12-12 2017-11-17 深圳华大基因研究院 基因测序仪
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
CA2998169A1 (en) 2015-09-18 2017-03-23 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
RU2593943C1 (ru) * 2015-09-24 2016-08-10 Общество с ограниченной ответственностью "БИОССЕТ" Аппарат для синтеза олиго(поли)нуклеотидов
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
US11550939B2 (en) 2017-02-22 2023-01-10 Twist Bioscience Corporation Nucleic acid based data storage using enzymatic bioencryption
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
EP3638782A4 (de) 2017-06-12 2021-03-17 Twist Bioscience Corporation Verfahren für nahtlose nukleinsäureanordnung
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
KR20240024357A (ko) 2017-10-20 2024-02-23 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
EP3814497A4 (de) 2018-05-18 2022-03-02 Twist Bioscience Corporation Polynukleotide, reagenzien und verfahren zur nukleinsäurehybridisierung
SG11202109283UA (en) 2019-02-26 2021-09-29 Twist Bioscience Corp Variant nucleic acid libraries for antibody optimization
US11724261B1 (en) * 2019-05-13 2023-08-15 Integrated Dna Technologies, Inc. Synthesizer system with inflatable seal and valve arrangement
EP3987019A4 (de) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-basierte nukleinsäuresequenzanordnung
WO2021254934A1 (en) * 2020-06-16 2021-12-23 Dna Script Systems, apparatus and kits for enzymatic polynucleotide synthesis
US11666882B2 (en) 2020-06-26 2023-06-06 Biolytic Lab Performance, Inc. Bidirectional flow reaction system for solid phase synthesis
US11473699B2 (en) 2020-06-26 2022-10-18 Biolytic Lab Performance, Inc Tubing support system
US11970697B2 (en) 2020-10-19 2024-04-30 Twist Bioscience Corporation Methods of synthesizing oligonucleotides using tethered nucleotides

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3531258A (en) * 1967-11-16 1970-09-29 Us Health Education & Welfare Apparatus for the automated synthesis of peptides
GB2118189B (en) * 1982-02-26 1985-05-15 Shimadzu Corp An automatic synthesizer for dna
US4517338A (en) * 1983-06-20 1985-05-14 Chiron Corporation Multiple reactor system and method for polynucleotide synthesis
US4746490A (en) * 1983-09-22 1988-05-24 Saneii Hossain H Solid phase peptide synthesizer
JPS60105691A (ja) * 1983-11-14 1985-06-11 Nippon Zeon Co Ltd ポリヌクレオチド合成装置
US4690165A (en) * 1984-11-29 1987-09-01 Beckman Instruments, Inc. Mixing tee check valve and method
US4701304A (en) * 1985-04-19 1987-10-20 Applied Protein Technologies, Inc. Apparatus for automated synthesis of peptides
FR2582655B1 (fr) * 1985-06-03 1988-12-23 Centre Nat Rech Scient Multi-synthetiseur de peptides semi-automatique en phase solide
AU596987B2 (en) * 1985-08-30 1990-05-24 Tosoh Corporation Automated immunoassay analyser
US5104621A (en) * 1986-03-26 1992-04-14 Beckman Instruments, Inc. Automated multi-purpose analytical chemistry processing center and laboratory work station
US4811218A (en) * 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
US5175209A (en) * 1987-01-06 1992-12-29 Baylor College Of Medicine Porous wafer for segmented synthesis of biopolymers
US4861866A (en) * 1987-01-21 1989-08-29 Eldex Laboratories, Inc. Continuous flow peptide synthesizer
JPH0790163B2 (ja) * 1987-08-31 1995-10-04 株式会社島津製作所 圧送式液体供給装置
DK492788D0 (da) * 1988-09-02 1988-09-05 Morten Meldal Peptidsynteseapparat
JPH03502737A (ja) * 1988-11-08 1991-06-20 アプライド・バイオシステムズ，インコーポレーテッド アッセイオメイト
US5112575A (en) * 1988-12-16 1992-05-12 Eastman Kodak Company Polynucleotide synthesizer
US5053454A (en) * 1989-02-15 1991-10-01 Sri International Multiple polymer synthesizer
US5213761A (en) * 1989-04-12 1993-05-25 Olympus Optical Co., Ltd. Automatic chemical analyzer having an improved delivery mechanism

Also Published As

Publication number Publication date
EP0683790A4 (de) 1996-03-13
US5368823A (en) 1994-11-29
DE69424151D1 (de) 2000-05-31
US5541314A (en) 1996-07-30
WO1994018226A1 (en) 1994-08-18
EP0683790B1 (de) 2000-04-26
JPH08506813A (ja) 1996-07-23
EP0683790A1 (de) 1995-11-29

Similar Documents

Publication Publication Date Title
DE69424151D1 (de) Automatisierte synthese von oligonukleotiden
DE69605282T2 (de) Synthese von cyclopropylacetylen
DE69924930D1 (de) Synthese von Oligonukleotiden
ATE196463T1 (de) Assymetrische synthese von (-) 6-chloro-4-
DE69330129T2 (de) Synthese von 1,4,7-thiazacyclononan-derivaten
ATE123037T1 (de) Synthese von carbonylverbindungen.
DE69505642D1 (de) Synthese von cyclopentadienyl - metall - koordinationskomplexen aus metallhydrocarbyloxiden
DE69501534D1 (de) Synthese von 1,1,1,3,3-Pentafluorpropan
DE68908440T2 (de) Synthese von Trichostatin.
DE69310372T2 (de) Synthesereaktionssäule
ATE231507T1 (de) Synthese von azyklischen nukleosiden
DE69801344T2 (de) Synthese von Bis(Phthalocyanylalumino)tetraphenyldisiloxanen
DE69602937D1 (de) Verbesserte synthese von racemischen metallocenen
DE69722585D1 (de) Synthese von wellenformen
DE69627349D1 (de) Einrichtung zur synthese von oligonukleotiden
DE69226706T2 (de) Synthese von smektit-tonmineralen
DE69410814D1 (de) Synthese von sphingosinen
DE69624759D1 (de) Synthese von Difluormethan
DE69422357D1 (de) Schmelzverfahren zur Synthese von Zinn(II)-terephthalat
DE69603578T2 (de) Synthese von tetrafluorethylen
DE69310997D1 (de) Prillverfahren
DE69201245D1 (de) Verfahren zur Synthese von Hydrogenoperfluoralkanen.
DE69707656T2 (de) Synthese von cycloalkyldiarylphosphin
DE69318188T3 (de) Synthese von vinylestern
DE69708273T2 (de) Synthese von cycloalkyldiarylphosphin

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: VONNEMANN, KLOIBER & KOLLEGEN, 80796 MUENCHEN