DE69402863D1 - Regulierung der stellen-spezifischen rekombination durch stellen-spezifische fusionsproteine, die aus rekombinase und nuklearem rezeptor bestehen - Google Patents

Regulierung der stellen-spezifischen rekombination durch stellen-spezifische fusionsproteine, die aus rekombinase und nuklearem rezeptor bestehen

Info

Publication number
DE69402863D1
DE69402863D1 DE69402863T DE69402863T DE69402863D1 DE 69402863 D1 DE69402863 D1 DE 69402863D1 DE 69402863 T DE69402863 T DE 69402863T DE 69402863 T DE69402863 T DE 69402863T DE 69402863 D1 DE69402863 D1 DE 69402863D1
Authority
DE
Germany
Prior art keywords
job
recombinase
recombination
fusion proteins
regulation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69402863T
Other languages
English (en)
Other versions
DE69402863T2 (de
Inventor
Francis Stewart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Europaisches Laboratorium fuer Molekularbiologie EMBL
Original Assignee
Europaisches Laboratorium fuer Molekularbiologie EMBL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europaisches Laboratorium fuer Molekularbiologie EMBL filed Critical Europaisches Laboratorium fuer Molekularbiologie EMBL
Publication of DE69402863D1 publication Critical patent/DE69402863D1/de
Application granted granted Critical
Publication of DE69402863T2 publication Critical patent/DE69402863T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/721—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69402863T 1993-06-28 1994-06-28 Regulierung der stellen-spezifischen rekombination durch stellen-spezifische fusionsproteine, die aus rekombinase und nuklearem rezeptor bestehen Expired - Lifetime DE69402863T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP93110298A EP0632054A1 (de) 1993-06-28 1993-06-28 Regulation stellenspezifischer Rekombination mittels stellungsspezifischer Rekombinase/Kernrezeptor-Fusionsproteine
PCT/EP1994/002088 WO1995000555A1 (en) 1993-06-28 1994-06-28 Regulation of site-specific recombination by site-specific recombinase/nuclear receptor fusion proteins

Publications (2)

Publication Number Publication Date
DE69402863D1 true DE69402863D1 (de) 1997-05-28
DE69402863T2 DE69402863T2 (de) 1997-07-31

Family

ID=8213015

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69402863T Expired - Lifetime DE69402863T2 (de) 1993-06-28 1994-06-28 Regulierung der stellen-spezifischen rekombination durch stellen-spezifische fusionsproteine, die aus rekombinase und nuklearem rezeptor bestehen

Country Status (9)

Country Link
US (1) US6040430A (de)
EP (2) EP0632054A1 (de)
JP (1) JP2986915B2 (de)
AT (1) ATE152123T1 (de)
AU (1) AU7227994A (de)
CA (1) CA2166198C (de)
DE (1) DE69402863T2 (de)
ES (1) ES2101552T3 (de)
WO (1) WO1995000555A1 (de)

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US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6964861B1 (en) * 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
PT937098E (pt) * 1995-06-07 2002-12-31 Invitrogen Corp Clonagem recombinatoria in vitro utilizando locais de recombinacao modificados
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
FR2749857B1 (fr) * 1996-06-12 1998-08-14 Centre Nat Rech Scient Generation de molecules replicatives in vivo
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
CN100342008C (zh) * 1997-10-24 2007-10-10 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
BR9814669A (pt) 1997-11-18 2001-11-20 Pioneer Hi Bred Int Método para a integração de dna estranho emgenomas eucarióticos
CA2306184C (en) 1997-11-18 2007-05-15 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
US6720475B1 (en) 1997-11-18 2004-04-13 Pioneer Hi-Bred International, Inc. Modified nucleic acid sequence encoding FLP recombinase
US7102055B1 (en) 1997-11-18 2006-09-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant
US5929301A (en) 1997-11-18 1999-07-27 Pioneer Hi-Bred International Nucleic acid sequence encoding FLP recombinase
NZ504510A (en) 1997-11-18 2002-10-25 Pioneer Hi Bred Int Methods and compositions for increasing efficiency of excision of a viral replicon from T-DNA that is transferred to a plant by agroinfection
CA2363924A1 (en) 1999-03-02 2000-09-08 Invitrogen Corporation Compositions and methods for use in recombinational cloning of nucleic acids
GB9924604D0 (en) * 1999-10-18 1999-12-22 European Molecular Biology Lab Embl Genetic switch
DE19956272B4 (de) * 1999-11-23 2004-09-16 Mpb Cologne Gmbh Molecular Plant & Protein Biotechnology Verfahren zur gesteuerten Nach-Ernte-Produktion von Proteinen in Wirtsorganismen
AU785483B2 (en) 1999-12-10 2007-09-20 Invitrogen Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
EP1118668A1 (de) * 2000-01-07 2001-07-25 ARTEMIS Pharmaceuticals GmbH Übertragung von Rekombinasen zur induzierbaren Genveränderung
DE10024740A1 (de) * 2000-05-19 2001-11-29 Mpb Cologne Gmbh Molecular Pla Verfahren zur gesteuerten Eliminierung gewünschter DNA-Sequenzen in Wirtsorganismen
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
DE10038573A1 (de) * 2000-08-03 2002-02-21 Mpb Cologne Gmbh Molecular Pla Verfahren zur Selektion auf Wirtszellen mit eliminierten DNA-Sequenzen
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
FR2814642B1 (fr) * 2000-10-03 2005-07-01 Ass Pour Le Dev De La Rech En Souris transgenique pour la recombinaison ciblee mediee par la cre-er modifiee
EP1322152A2 (de) * 2000-10-03 2003-07-02 ADEREGEM (Association pour le Développement de la Recherche en Génétique Moléculaire Transgene maus zur gezielten rekombination vermittelt durch verändertes cre-er
US7560622B2 (en) 2000-10-06 2009-07-14 Pioneer Hi-Bred International, Inc. Methods and compositions relating to the generation of partially transgenic organisms
EP1390394A4 (de) * 2001-04-19 2004-05-26 Invitrogen Corp Zusammensetzungen und verfahren zur rekombinationsklonierung von nukleinsäuremolekülen
WO2002095055A2 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
EP1264891A1 (de) * 2001-05-31 2002-12-11 Plant Research International B.V. Veränderung des Pflanzen Genoms durch induzierbaren, ortsspezifischen Rekombination von Transgenen
US7351877B2 (en) 2002-03-29 2008-04-01 Syngenta Participations Ag Lambda integrase mediated recombination in plants
WO2004009768A2 (en) * 2002-07-18 2004-01-29 Invitrogen Corporation Viral vectors containing recombination sites
US20050095615A1 (en) * 2003-06-26 2005-05-05 Welch Peter J. Methods and compositions for detecting promoter activity and expressing fusion proteins
DE602004027538D1 (de) * 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US7935862B2 (en) 2003-12-02 2011-05-03 Syngenta Participations Ag Targeted integration and stacking of DNA through homologous recombination
JP4485475B2 (ja) * 2004-02-12 2010-06-23 独立行政法人科学技術振興機構 核内レセプターのアゴニスト・アンタゴニスト検出用プローブとそれを用いた核内レセプターに対するアゴニストおよびアンタゴニストのスクリーニング方法
US9206397B2 (en) * 2006-07-07 2015-12-08 The Fred Hutchinson Cancer Research Center High efficiency FLP site-specific recombination in mammalian cells using an optimized FLP gene
US9234193B2 (en) 2011-05-31 2016-01-12 Keygene N.V. Pest resistant plants
US20150059018A1 (en) 2011-10-19 2015-02-26 Keygene N.V. Methods and compositions for producing drimenol
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
US11236346B2 (en) 2015-09-04 2022-02-01 Keygene N.V. Diplospory gene
CN113874388A (zh) 2019-05-29 2021-12-31 主基因有限公司 孤雌生殖基因
KR20230088741A (ko) 2020-10-13 2023-06-20 키진 엔.브이. 단위생식 유전자의 변형된 프로모터

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0146050B1 (de) * 1983-12-16 1990-07-04 Denis M. Callewaert Stellenselektiver Plasminogenaktivator und Verfahren zu seiner Herstellung und Verwendung
WO1993015191A1 (en) * 1992-01-24 1993-08-05 Life Technologies, Inc. Modulation of enzyme activities in the in vivo cloning of dna
US5589362A (en) * 1993-06-14 1996-12-31 Basf Aktiengesellschaft Tetracycline regulated transcriptional modulators with altered DNA binding specificities

Also Published As

Publication number Publication date
ES2101552T3 (es) 1997-07-01
US6040430A (en) 2000-03-21
ATE152123T1 (de) 1997-05-15
EP0707599A1 (de) 1996-04-24
JPH08511681A (ja) 1996-12-10
CA2166198C (en) 2002-01-08
JP2986915B2 (ja) 1999-12-06
EP0707599B1 (de) 1997-04-23
WO1995000555A1 (en) 1995-01-05
CA2166198A1 (en) 1995-01-05
EP0632054A1 (de) 1995-01-04
DE69402863T2 (de) 1997-07-31
AU7227994A (en) 1995-01-17

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