DE69328459D1 - Vorbereitung von Nukleinsäure aus mononuklearen Zellen - Google Patents

Vorbereitung von Nukleinsäure aus mononuklearen Zellen

Info

Publication number
DE69328459D1
DE69328459D1 DE69328459T DE69328459T DE69328459D1 DE 69328459 D1 DE69328459 D1 DE 69328459D1 DE 69328459 T DE69328459 T DE 69328459T DE 69328459 T DE69328459 T DE 69328459T DE 69328459 D1 DE69328459 D1 DE 69328459D1
Authority
DE
Germany
Prior art keywords
nucleic acid
cells
preparation
mononuclear cells
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69328459T
Other languages
English (en)
Other versions
DE69328459T2 (de
Inventor
Thomas B Ryder
Daniel Louis Kacian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of DE69328459D1 publication Critical patent/DE69328459D1/de
Publication of DE69328459T2 publication Critical patent/DE69328459T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE69328459T 1992-06-08 1993-06-08 Vorbereitung von Nukleinsäure aus mononuklearen Zellen Expired - Fee Related DE69328459T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US89558792A 1992-06-08 1992-06-08

Publications (2)

Publication Number Publication Date
DE69328459D1 true DE69328459D1 (de) 2000-05-31
DE69328459T2 DE69328459T2 (de) 2000-09-07

Family

ID=25404721

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69328459T Expired - Fee Related DE69328459T2 (de) 1992-06-08 1993-06-08 Vorbereitung von Nukleinsäure aus mononuklearen Zellen

Country Status (11)

Country Link
US (1) US5639599A (de)
EP (1) EP0574227B1 (de)
JP (1) JP3494646B2 (de)
KR (1) KR950701979A (de)
AT (1) ATE192196T1 (de)
AU (2) AU687352B2 (de)
CA (1) CA2137563C (de)
DE (1) DE69328459T2 (de)
DK (1) DK0574227T3 (de)
ES (1) ES2145030T3 (de)
WO (1) WO1993025710A1 (de)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
US5789148A (en) * 1994-08-31 1998-08-04 Dendreon Corporation Cell separation composition
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5571673A (en) * 1994-11-23 1996-11-05 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
CA2239729C (en) * 1995-12-11 2006-10-31 Dendreon Corporation Cell separation composition, kit and method
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US20050287583A1 (en) * 1997-01-21 2005-12-29 Promega Corporation Methods and kits for isolating biological target materials using silica magnetic particles
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
ATE423622T1 (de) 1998-05-01 2009-03-15 Gen Probe Inc Automatisches isolierungs- und amplifizierungsverfahren für eine zielnukleinsäuresequenz
US6268127B1 (en) 1999-02-03 2001-07-31 Ortho-Clinical Diagnostics, Inc. Method for preparing DNA from serum and plasma
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) * 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US8043834B2 (en) * 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US7465562B2 (en) * 2003-05-19 2008-12-16 Brandeis University Nucleic acid processing methods, kits and devices
US20050130177A1 (en) 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US7939249B2 (en) * 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
AU2005280162B2 (en) 2004-08-27 2012-04-26 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
EP2322940B1 (de) 2005-03-10 2014-10-29 Gen-Probe Incorporated Systeme und Verfahren zur Durchführung von Tests zum Nachweis oder zur Quantifizierung von Analyten in Proben
EP1909108B1 (de) 2005-03-10 2019-05-29 Gen-Probe Incorporated Systeme und Verfahren zur Durchführung von Tests zum Nachweis oder zur Quantifizierung von Analyten
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
EP1978111B1 (de) 2007-04-02 2013-03-27 Gen-Probe Incorporated Zusammensetzungen, Kits und zugehörige Verfahren zur Erkennung und/oder Überwachung von Pseudomonas aeruginosa
US8703492B2 (en) * 2007-04-06 2014-04-22 Qiagen Gaithersburg, Inc. Open platform hybrid manual-automated sample processing system
US8357538B2 (en) * 2007-04-06 2013-01-22 Qiagen Gaithersburg, Inc. Automated assay and system
US8703421B2 (en) 2008-05-30 2014-04-22 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Salmonella
WO2010078374A1 (en) 2008-12-30 2010-07-08 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of listeria
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
WO2011063139A1 (en) 2009-11-18 2011-05-26 Qiagen Laboratory central control unit method and system
EP2752671A3 (de) 2010-07-23 2016-08-24 Beckman Coulter, Inc. System oder Verfahren zur Aufnahme analytischer Einheiten
CN104040352B (zh) 2011-11-07 2016-09-07 贝克曼考尔特公司 机械臂
EP2776844B1 (de) 2011-11-07 2020-09-30 Beckman Coulter, Inc. Probenbehälterdetektion
EP2776843B1 (de) 2011-11-07 2019-03-27 Beckman Coulter, Inc. Zentrifugensystem und arbeitsablauf
JP2014532881A (ja) 2011-11-07 2014-12-08 ベックマン コールター， インコーポレイテッド 標本輸送システムのための磁気制動
BR112014010955A2 (pt) 2011-11-07 2017-06-06 Beckman Coulter Inc sistema e método para processar amostras
JP6190380B2 (ja) 2011-11-07 2017-08-30 ベックマン コールター， インコーポレイテッド 等分機システムおよびワークフロー
GB201618106D0 (en) * 2016-10-26 2016-12-07 Lift Biosciences Ltd Cancer-killing cells
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3852424A (en) * 1957-07-26 1974-12-03 Ciba Geigy Corp Purified ferrimycin and process for obtaining same
US3957435A (en) * 1974-12-12 1976-05-18 Miles Laboratories, Inc. Passive hemagglutination test method and composition for use therein
US4767708A (en) 1984-08-07 1988-08-30 Carnegie Mellon University Enzyme amplification and purification
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4863964A (en) * 1985-07-02 1989-09-05 Biomedical Frontiers, Inc. Method for the stabilization of deferoxamine to chelate free ions in physiological fluid
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
ES2074051T3 (es) * 1987-09-21 1995-09-01 Gen Probe Inc Ensayo de proteccion homogeneo.
JP2791367B2 (ja) * 1988-04-21 1998-08-27 マイクロプローブ・コーポレーション 核酸抽出方法
CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5480784A (en) * 1989-07-11 1996-01-02 Gen-Probe Incorporated Nucleic acid sequence amplification methods
US5284940A (en) * 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
JPH07504801A (ja) * 1991-08-06 1995-06-01 ザ　ワールド　ヘルス　オーガニゼイション Ｄｎａの単離方法

Also Published As

Publication number Publication date
CA2137563A1 (en) 1993-12-23
AU5207498A (en) 1998-03-12
EP0574227B1 (de) 2000-04-26
EP0574227A2 (de) 1993-12-15
ATE192196T1 (de) 2000-05-15
DE69328459T2 (de) 2000-09-07
WO1993025710A1 (en) 1993-12-23
AU706083B2 (en) 1999-06-10
JP3494646B2 (ja) 2004-02-09
JPH07507458A (ja) 1995-08-24
AU5207698A (en) 1998-04-23
KR950701979A (ko) 1995-05-17
DK0574227T3 (da) 2000-08-07
AU4530893A (en) 1994-01-04
AU687352B2 (en) 1998-02-26
AU713753B2 (en) 1999-12-09
CA2137563C (en) 2005-06-28
US5639599A (en) 1997-06-17
EP0574227A3 (en) 1994-08-24
ES2145030T3 (es) 2000-07-01

Similar Documents

Publication Publication Date Title
DE69328459D1 (de) Vorbereitung von Nukleinsäure aus mononuklearen Zellen
CA2451985A1 (en) Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection
Guengerich et al. Rat and human liver microsomal epoxide hydratase (EC 4.2. 1.63): Purification and evidence for the existence of multiple forms
EP0188450A4 (de) Verfahren zum in vitro-nachweis und bestimmung unbekannter pathogene oder genetischer einheiten.
DE69802191D1 (de) Festphase nukleinsäure-isolierung
CA2084533A1 (en) Process for lysing mycobacteria
AU9058091A (en) Preparation of nucleic acid samples
DE68917736D1 (de) System zur magnetischen affinitäts-separation von zellen aus zellkonzentraten.
DE60129000D1 (de) Verfahren und mittel zur Isolierung von Mikroorganismen
ATE242780T1 (de) Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür
DE69632466D1 (de) Lösung die chaotropische mittel enthält und verwendung dieser lösung in verfahren zur isolierung von dna, rna und proteinen
GB0013658D0 (en) Nucleic acid isolation
WO2003020877A3 (en) Staining and sorting genomes and chromosomes using sequence-specific polyamides
WO2010003493A1 (de) Schnelles analyseverfahren biologischer mischproben
DK0402997T3 (da) Fremgangsmåde til bestemmelse af nukleinsyre
CA2199835A1 (en) Method for preparing nucleic acids for analysis and kits useful therefor
SE8401125L (sv) Sett vid diagnostisering in vitro och diagnostiskt medel
CA2533440A1 (en) Amplifying atp with adp-free polyphosphate-adenylate kinase fusion protein
Fulton et al. Methemoglobin reductase in three species of Bovidae
WO2000036420A3 (en) Differential expression in primary breast cancer
JP2016524921A (ja) 核の保存方法
Isobe et al. An assay for ribonuclease activity, based on ultraviolet absorption of RNA hydrolysate, using phosphotungstic acid
EP0089826A3 (de) Verfahren zur Spezifizierung serologisch ausgewählter Zell-Populationen
Khesin et al. Ribonuclease activity in preparations of human leukocytic interferon
WO1997037040A3 (en) Isolation and/or amplification of hepatitis c virus (hcv) nucleic acids from samples suspected to contain hcv

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee