DE69114353D1 - Vorbereitung und Isolierung von einzel-strängigen Nukleinsäuren mit Avidin-Biotintrennung. - Google Patents

Vorbereitung und Isolierung von einzel-strängigen Nukleinsäuren mit Avidin-Biotintrennung.

Info

Publication number
DE69114353D1
DE69114353D1 DE69114353T DE69114353T DE69114353D1 DE 69114353 D1 DE69114353 D1 DE 69114353D1 DE 69114353 T DE69114353 T DE 69114353T DE 69114353 T DE69114353 T DE 69114353T DE 69114353 D1 DE69114353 D1 DE 69114353D1
Authority
DE
Germany
Prior art keywords
avidin
nucleic acid
nucleic acids
isolation
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69114353T
Other languages
English (en)
Other versions
DE69114353T2 (de
Inventor
Annie Liu Wu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Johnson and Johnson Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson and Johnson Clinical Diagnostics Inc filed Critical Johnson and Johnson Clinical Diagnostics Inc
Publication of DE69114353D1 publication Critical patent/DE69114353D1/de
Application granted granted Critical
Publication of DE69114353T2 publication Critical patent/DE69114353T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
DE69114353T 1990-05-04 1991-04-27 Vorbereitung und Isolierung von einzel-strängigen Nukleinsäuren mit Avidin-Biotintrennung. Expired - Fee Related DE69114353T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/519,533 US5387505A (en) 1990-05-04 1990-05-04 Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage

Publications (2)

Publication Number Publication Date
DE69114353D1 true DE69114353D1 (de) 1995-12-14
DE69114353T2 DE69114353T2 (de) 1996-04-18

Family

ID=24068718

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69114353T Expired - Fee Related DE69114353T2 (de) 1990-05-04 1991-04-27 Vorbereitung und Isolierung von einzel-strängigen Nukleinsäuren mit Avidin-Biotintrennung.

Country Status (10)

Country Link
US (1) US5387505A (de)
EP (1) EP0456304B1 (de)
JP (1) JPH04228076A (de)
KR (1) KR910020180A (de)
AT (1) ATE130048T1 (de)
CA (1) CA2039222A1 (de)
DE (1) DE69114353T2 (de)
FI (1) FI912141A (de)
HK (1) HK38296A (de)
IE (1) IE911508A1 (de)

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EP2109669B1 (de) * 2006-11-15 2015-01-07 Life Technologies AS Verfahren zur reversiblen bindung einer biotin-verbindung an einen träger
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JP5437639B2 (ja) * 2006-12-28 2014-03-12 日本たばこ産業株式会社 耐熱性ビオチン結合性タンパク質の利用法、および当該タンパク質が結合した固体担体
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Also Published As

Publication number Publication date
CA2039222A1 (en) 1991-11-05
ATE130048T1 (de) 1995-11-15
FI912141A (fi) 1991-11-05
EP0456304A1 (de) 1991-11-13
KR910020180A (ko) 1991-12-19
HK38296A (en) 1996-03-15
JPH04228076A (ja) 1992-08-18
IE911508A1 (en) 1991-11-06
DE69114353T2 (de) 1996-04-18
US5387505A (en) 1995-02-07
EP0456304B1 (de) 1995-11-08
FI912141A0 (fi) 1991-05-03

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee