DE69114353D1 - Vorbereitung und Isolierung von einzel-strängigen Nukleinsäuren mit Avidin-Biotintrennung. - Google Patents

Vorbereitung und Isolierung von einzel-strängigen Nukleinsäuren mit Avidin-Biotintrennung.

Info

Publication number
DE69114353D1
DE69114353D1 DE69114353T DE69114353T DE69114353D1 DE 69114353 D1 DE69114353 D1 DE 69114353D1 DE 69114353 T DE69114353 T DE 69114353T DE 69114353 T DE69114353 T DE 69114353T DE 69114353 D1 DE69114353 D1 DE 69114353D1
Authority
DE
Germany
Prior art keywords
avidin
nucleic acid
nucleic acids
isolation
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69114353T
Other languages
English (en)
Other versions
DE69114353T2 (de
Inventor
Annie Liu Wu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Johnson and Johnson Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson and Johnson Clinical Diagnostics Inc filed Critical Johnson and Johnson Clinical Diagnostics Inc
Publication of DE69114353D1 publication Critical patent/DE69114353D1/de
Application granted granted Critical
Publication of DE69114353T2 publication Critical patent/DE69114353T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Peptides Or Proteins (AREA)
DE69114353T 1990-05-04 1991-04-27 Vorbereitung und Isolierung von einzel-strängigen Nukleinsäuren mit Avidin-Biotintrennung. Expired - Fee Related DE69114353T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/519,533 US5387505A (en) 1990-05-04 1990-05-04 Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage

Publications (2)

Publication Number Publication Date
DE69114353D1 true DE69114353D1 (de) 1995-12-14
DE69114353T2 DE69114353T2 (de) 1996-04-18

Family

ID=24068718

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69114353T Expired - Fee Related DE69114353T2 (de) 1990-05-04 1991-04-27 Vorbereitung und Isolierung von einzel-strängigen Nukleinsäuren mit Avidin-Biotintrennung.

Country Status (10)

Country Link
US (1) US5387505A (de)
EP (1) EP0456304B1 (de)
JP (1) JPH04228076A (de)
KR (1) KR910020180A (de)
AT (1) ATE130048T1 (de)
CA (1) CA2039222A1 (de)
DE (1) DE69114353T2 (de)
FI (1) FI912141A (de)
HK (1) HK38296A (de)
IE (1) IE911508A1 (de)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6190864B1 (en) * 1991-05-08 2001-02-20 Chiron Corporation HCV genomic sequences for diagnostics and therapeutics
FR2679252B1 (fr) * 1991-07-17 1993-12-10 Bio Merieux Systeme de sondes permettant d'effectuer le typage hla dr, et procede de typage utilisant lesdites sondes.
US5976789A (en) * 1991-07-17 1999-11-02 Bio Merieux System of probes enabling HLA-DR typing to be performed, and typing method using said probes
US5401632A (en) * 1992-07-16 1995-03-28 Wisconsin Alumni Research Foundation Triple helix purification and sequencing
US6194144B1 (en) 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
US7803529B1 (en) 1995-04-11 2010-09-28 Sequenom, Inc. Solid phase sequencing of biopolymers
US5616465A (en) 1995-08-09 1997-04-01 The Regents Of The University Of California Detection and isolation of nucleic acid sequences using competitive hybridization probes
US6027879A (en) * 1995-08-09 2000-02-22 The Regents Of The University Of California Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe
ATE346165T1 (de) * 1995-11-29 2006-12-15 Anthony Nolan Bone Marrow Trus Verfahren zur trennung und/oder erkennung von dns-molekülen
US6022688A (en) * 1996-05-13 2000-02-08 Sequenom, Inc. Method for dissociating biotin complexes
EP1460083B1 (de) 1996-11-06 2006-01-18 Sequenom, Inc. Verfahren zur Analyse und Vorrichtung
US6133436A (en) * 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6046005A (en) * 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
IL120337A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Method for joining DNA fragments
US6268131B1 (en) 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
US6723564B2 (en) 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
EP1990428B1 (de) * 2000-02-07 2010-12-22 Illumina, Inc. Nukleinsäuredetektionsverfahren mit universellem Priming
US8076063B2 (en) * 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) * 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US20030082549A1 (en) * 2000-08-30 2003-05-01 Xiangjun Liu Method for determining alleles
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
GB0102568D0 (en) 2001-02-01 2001-03-21 Magnetic Biosolutions Sweden A Method
JP2002333446A (ja) * 2001-05-09 2002-11-22 Fuji Photo Film Co Ltd 生物学的素材チップ
WO2003062444A2 (en) * 2001-11-13 2003-07-31 Emory University Array systems and methods
CA2467814A1 (en) * 2001-12-03 2003-06-12 Illumina, Inc. Multiplexed methylation detection methods
US7108976B2 (en) * 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
US9388459B2 (en) * 2002-06-17 2016-07-12 Affymetrix, Inc. Methods for genotyping
US20040259105A1 (en) * 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
US7459273B2 (en) * 2002-10-04 2008-12-02 Affymetrix, Inc. Methods for genotyping selected polymorphism
US8114978B2 (en) 2003-08-05 2012-02-14 Affymetrix, Inc. Methods for genotyping selected polymorphism
US20060134612A1 (en) * 2004-02-10 2006-06-22 Frederic Bushman Methods and compositions for retroviral preintegration complex-based assays
US20070248958A1 (en) * 2004-09-15 2007-10-25 Microchip Biotechnologies, Inc. Microfluidic devices
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
JP2006219399A (ja) * 2005-02-09 2006-08-24 Noguchi Inst 水溶性糖鎖プローブ
JP2006230342A (ja) * 2005-02-28 2006-09-07 Tokyo Univ Of Agriculture & Technology 一本鎖核酸の分離方法および装置並びにマイクロアレイおよびｄｎａチップ。
US7452671B2 (en) * 2005-04-29 2008-11-18 Affymetrix, Inc. Methods for genotyping with selective adaptor ligation
US11306351B2 (en) 2005-12-21 2022-04-19 Affymetrix, Inc. Methods for genotyping
CA2641271A1 (en) * 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
CN101622340A (zh) * 2006-11-15 2010-01-06 茵维特罗根戴纳股份公司 生物素化合物与支持物可逆结合的方法
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
CA2673968A1 (en) 2006-12-28 2008-07-10 Japan Tobacco, Inc. Use of heat-resistant biotin-binding protein, and solid support having the protein attached thereto
JP5437639B2 (ja) * 2006-12-28 2014-03-12 日本たばこ産業株式会社 耐熱性ビオチン結合性タンパク質の利用法、および当該タンパク質が結合した固体担体
KR20100028526A (ko) 2007-02-05 2010-03-12 마이크로칩 바이오테크놀로지스, 인크. 마이크로유체 및 나노유체 장치, 시스템 및 응용
CA2680588A1 (en) 2007-03-26 2008-10-02 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US20080293589A1 (en) * 2007-05-24 2008-11-27 Affymetrix, Inc. Multiplex locus specific amplification
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
WO2009032779A2 (en) * 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for the size-specific seperation of nucleic acid from a sample
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
WO2009108260A2 (en) 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
US9074244B2 (en) * 2008-03-11 2015-07-07 Affymetrix, Inc. Array-based translocation and rearrangement assays
WO2009120808A2 (en) 2008-03-26 2009-10-01 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
EP2384429A1 (de) * 2008-12-31 2011-11-09 Integenx Inc. Instrument mit mikrofluidischem chip
EP2408936A4 (de) * 2009-03-18 2013-01-30 Sequenom Inc Verwendung wärmestabiler endonukleasen zur herstellung von reportermolekülen
CN102459565A (zh) * 2009-06-02 2012-05-16 尹特根埃克斯有限公司 具有隔膜阀的流控设备
RU2559541C2 (ru) 2009-06-05 2015-08-10 Интедженкс Инк. Универсальная система подготовки образцов и применение в интегрированной системе анализа
US8584703B2 (en) * 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
EP2606154B1 (de) 2010-08-20 2019-09-25 Integenx Inc. Integriertes analysesystem
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
WO2015073999A1 (en) 2013-11-18 2015-05-21 Integenx Inc. Cartridges and instruments for sample analysis
US10208332B2 (en) 2014-05-21 2019-02-19 Integenx Inc. Fluidic cartridge with valve mechanism
CN113092563B (zh) 2014-10-22 2024-06-07 尹特根埃克斯有限公司 用于样品制备、处理和分析的系统和方法
JP2023512372A (ja) * 2020-02-03 2023-03-27 イルミナ インコーポレイテッド ビオチン－ストレプトアビジン切断組成物及びライブラリ断片切断

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4772691A (en) * 1985-06-05 1988-09-20 The Medical College Of Wisconsin, Inc. Chemically cleavable nucleotides
AU6834187A (en) * 1985-12-17 1987-07-15 Genetics Institute Inc. Displacement polynucleotide method and reagent complex
US4818680A (en) * 1985-12-18 1989-04-04 Mary Collins Method and kit involving displacement and rehybridization of labeled polynucleotide
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
JPH07107537B2 (ja) * 1987-08-03 1995-11-15 イーストマン コダック カンパニー アビジン−及びビオチン−固定試薬，分析要素並びにその使用法
US4914210A (en) * 1987-10-02 1990-04-03 Cetus Corporation Oligonucleotide functionalizing reagents
EP0322311B1 (de) * 1987-12-21 1994-03-23 Applied Biosystems, Inc. Verfahren und Testsatz zum Nachweis einer Nukleinsäure-Sequenz
SE8801070D0 (sv) * 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
WO1990002205A1 (en) * 1988-08-25 1990-03-08 Angenics, Inc. Detection of nucleic acid sequences using particle agglutination
CA2002076A1 (en) * 1988-11-21 1990-05-21 Brent A. Burdick Diagnostic kit and method using a solid phase capture means for detecting nucleic acids

Also Published As

Publication number Publication date
US5387505A (en) 1995-02-07
ATE130048T1 (de) 1995-11-15
KR910020180A (ko) 1991-12-19
HK38296A (en) 1996-03-15
JPH04228076A (ja) 1992-08-18
EP0456304B1 (de) 1995-11-08
CA2039222A1 (en) 1991-11-05
FI912141A (fi) 1991-11-05
EP0456304A1 (de) 1991-11-13
IE911508A1 (en) 1991-11-06
DE69114353T2 (de) 1996-04-18
FI912141A0 (fi) 1991-05-03

Similar Documents

Publication Publication Date Title
DE69114353D1 (de) Vorbereitung und Isolierung von einzel-strängigen Nukleinsäuren mit Avidin-Biotintrennung.
DK0543942T3 (da) Homogent assaysystem
FI911152A0 (fi) Amplifikationsfoerfaranden av nukleinsyrasekvens.
CA2020958A1 (en) Nucleic acid sequence amplification methods
ATE156830T1 (de) Verfahren zur reinigung von nukleinsäuren
FI901917A0 (fi) Foerfarande foer extrahering, amplifering och detektering av en nukleinsyra ur helblod eller pbmc-fraktionen.
FR2650840B1 (fr) Procede rapide de detection et/ou d'identification d'une seule base sur une sequence d'acide nucleique, et ses applications
FI893526A0 (fi) Material och metod foer oekning av nukleinsyrahybridisering.
DE3750774D1 (de) Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe.
FI904494A0 (fi) Diagnos- och amplifieringsfoerfaranden foer eliminering av ett primer-maol-opar i primerns 3'-aenda.
ATE146529T1 (de) Hybridisierungsverfahren und reagenzsatz dafür
EP1113082A3 (de) Amplifikations festnahme Test
ATE123315T1 (de) Verfahren zur reinigung, vervielfachung und zum nachweis einer nukleinsäure.
ES2110916A1 (es) Procedimiento de preparacion de dianas para la reaccion en cadena de la polimerasa (pcr).
Wu Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage
EP1302547A3 (de) Verfahren zum Nachweis und Unterscheiden von Nukleinsäuresequenzen
NO875058D0 (no) Oligonukleotidprober og fremgangsmaate for detektering av nukleinsyrene i bakterier og andre levende organismer ved hybridisering.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee