DE69014723T2 - Zellzüchtungsverfahren. - Google Patents
Zellzüchtungsverfahren.Info
- Publication number
- DE69014723T2 DE69014723T2 DE69014723T DE69014723T DE69014723T2 DE 69014723 T2 DE69014723 T2 DE 69014723T2 DE 69014723 T DE69014723 T DE 69014723T DE 69014723 T DE69014723 T DE 69014723T DE 69014723 T2 DE69014723 T2 DE 69014723T2
- Authority
- DE
- Germany
- Prior art keywords
- cells
- sheaths
- culture
- cell
- microcarriers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000010261 cell growth Effects 0.000 title description 3
- 239000001963 growth medium Substances 0.000 claims abstract description 8
- 238000012258 culturing Methods 0.000 claims abstract description 3
- 229920002678 cellulose Polymers 0.000 claims description 12
- 239000001913 cellulose Substances 0.000 claims description 12
- 238000004113 cell culture Methods 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 3
- 238000010924 continuous production Methods 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 58
- 239000000463 material Substances 0.000 description 12
- 210000004962 mammalian cell Anatomy 0.000 description 5
- 239000012528 membrane Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 description 3
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 229940028334 follicle stimulating hormone Drugs 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 239000012876 carrier material Substances 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 230000008021 deposition Effects 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000012510 hollow fiber Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000007966 viscous suspension Substances 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 102000001938 Plasminogen Activators Human genes 0.000 description 1
- 108010001014 Plasminogen Activators Proteins 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000004931 aggregating effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004621 biodegradable polymer Substances 0.000 description 1
- 229920002988 biodegradable polymer Polymers 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 210000004754 hybrid cell Anatomy 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 229940127126 plasminogen activator Drugs 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920000747 poly(lactic acid) Polymers 0.000 description 1
- 229920000548 poly(silane) polymer Polymers 0.000 description 1
- 239000004626 polylactic acid Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 239000011232 storage material Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/10—Hollow fibers or tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
Landscapes
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Photovoltaic Devices (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL8901520 | 1989-06-16 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69014723D1 DE69014723D1 (de) | 1995-01-19 |
| DE69014723T2 true DE69014723T2 (de) | 1995-04-27 |
Family
ID=19854842
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69014723T Expired - Fee Related DE69014723T2 (de) | 1989-06-16 | 1990-06-12 | Zellzüchtungsverfahren. |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0403015B1 (fr) |
| JP (1) | JP2938937B2 (fr) |
| AT (1) | ATE115183T1 (fr) |
| AU (1) | AU631651B2 (fr) |
| CA (1) | CA2018992A1 (fr) |
| DE (1) | DE69014723T2 (fr) |
| DK (1) | DK0403015T3 (fr) |
| ES (1) | ES2067650T3 (fr) |
| GR (1) | GR3015318T3 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0541984A (ja) * | 1990-09-07 | 1993-02-23 | Dow Chem Co:The | 攪拌容器における中空繊維においての細胞の増殖 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0021257B1 (fr) * | 1979-06-28 | 1983-10-26 | Dr. Karl Thomae GmbH | Procédé pour la culture en surface de cellules à noyau et de production de produits dépendants de culture de cellules |
| US4748121A (en) * | 1984-11-30 | 1988-05-31 | Ppg Industries, Inc. | Porous glass fibers with immobilized biochemically active material |
| EP0289666B1 (fr) * | 1987-04-03 | 1992-06-17 | Yeda Research And Development Company Limited | Supports pour cultures de cellules |
| DE3733636C1 (de) * | 1987-10-05 | 1989-04-20 | Schott Glaswerke | Verfahren zum Vermehren von Zellen,insbesondere bei der Pruefung von Behandlungssubstanzen auf Wirksamkeit |
-
1990
- 1990-06-12 AT AT90201504T patent/ATE115183T1/de active
- 1990-06-12 DE DE69014723T patent/DE69014723T2/de not_active Expired - Fee Related
- 1990-06-12 EP EP90201504A patent/EP0403015B1/fr not_active Expired - Lifetime
- 1990-06-12 ES ES90201504T patent/ES2067650T3/es not_active Expired - Lifetime
- 1990-06-12 DK DK90201504.9T patent/DK0403015T3/da active
- 1990-06-14 CA CA002018992A patent/CA2018992A1/fr not_active Abandoned
- 1990-06-15 AU AU57523/90A patent/AU631651B2/en not_active Ceased
- 1990-06-15 JP JP2157392A patent/JP2938937B2/ja not_active Expired - Lifetime
-
1995
- 1995-03-07 GR GR950400496T patent/GR3015318T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU5752390A (en) | 1990-12-20 |
| DK0403015T3 (da) | 1995-05-15 |
| ES2067650T3 (es) | 1995-04-01 |
| EP0403015B1 (fr) | 1994-12-07 |
| GR3015318T3 (en) | 1995-06-30 |
| JPH0343072A (ja) | 1991-02-25 |
| DE69014723D1 (de) | 1995-01-19 |
| JP2938937B2 (ja) | 1999-08-25 |
| ATE115183T1 (de) | 1994-12-15 |
| AU631651B2 (en) | 1992-12-03 |
| CA2018992A1 (fr) | 1990-12-16 |
| EP0403015A1 (fr) | 1990-12-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0052252B1 (fr) | Méthode et appareil pour la culture submergée de cultures de cellules | |
| DE60026299T2 (de) | Vorrichtung zur züchtung von zellkulturen | |
| DE2319120C2 (de) | Züchten von Zellkulturen in vitro | |
| DE69731080T2 (de) | Kompartimentalisierungsverfahren zum screenen von mikroorganismen | |
| DE3504715C2 (fr) | ||
| DE2726313B2 (de) | Verfahren zur in-vitro-Biosynthese von Hormonen, insbesondere von Insulin | |
| DE68914412T2 (de) | Methode, System und Apparat zur Züchtung von Zellen. | |
| AT394576B (de) | Reaktor zur durchfuehrung biologischer reaktionen mittels biokatalysatoren | |
| DE69825002T2 (de) | Verfahren zur Verbesserung der Stabilität und/oder Haltbarkeit von verschiedenen Substraten | |
| DE69432932T2 (de) | Kontinuierlich arbeitende absetzvorrichtung | |
| EP2543719B1 (fr) | Bioréacteur avec meandre et procédé d'expansion, de différenciation et de récolte dynamiques de cellules hématopoïétiques | |
| DE3689400T2 (de) | Gefüllter mikroschwamm zur immobilisierung bioaktiver materialien. | |
| EP0224800B1 (fr) | Méthode et appareil pour la culture de cellules | |
| DE69014723T2 (de) | Zellzüchtungsverfahren. | |
| EP0263371A2 (fr) | Procédé et appareil pour la culture de cellules animales | |
| WO1987005322A1 (fr) | Procede et dispositif pour le traitement d'un melange liquide contenant un biocatalyseur ou des particules comparables | |
| DE102020102420A1 (de) | Gas-Flüssig-Reaktor zur blasenfreien Begasung einer Prozessflüssigkeit | |
| EP2342315A2 (fr) | Procédé de réduction de dépôts dans la culture d'organismes | |
| DE3504748C2 (fr) | ||
| EP1472336B1 (fr) | Procede et dispositif de culture de cellules en densites elevees et de preparation de produits a partir de ces cellules | |
| DE3786698T2 (de) | Immobilisierung von nichtverankerungsabhängigen Zellen. | |
| DE4123660A1 (de) | Neue traegermaterialien fuer die zellkultur | |
| DE102010005415B4 (de) | Verfahren und Vorrichtung zur dynamischen Expansion und/oder Differenzierung von suspendierten primären Zellen oder Stammzellen humanen und tierischen Ursprungs | |
| DE19756499C2 (de) | Mikrokapseln | |
| EP1981965B1 (fr) | Procédé de culture de cellules biologiques |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |